pCDNA3.1-GIT1-FLAG
$84.00
In stock
SKU
PL76855
NM_001085454.1
100μg liquid plasmid(Instantly):$105
| name | pCDNA3.1-GIT1-FLAG | another name | apCDNA3.1-GIT1-FLAG |
| Insert Size (bp) | 2313bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGGGAGTCA ATAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCATGTCCC GAAAGGGGCC GCGAGCGGAG GTGTGTGCGG ACTGCAGCGC CCCGGACCCT GGCTGGGCAT CCATCAGCAG GGGTGTGCTG GTGTGTGACG AGTGCTGCAG CGTGCACCGG AGCCTGGGAC GCCACATCTC CATTGTCAAG CACCTTCGCC ACAGCGCCTG GCCTCCCACG CTGCTGCAGA TGGTGCACAC GCTTGCCAGC AACGGGGCCA ACTCCATCTG GGAGCACTCC CTGCTGGACC CCGCACAAGT GCAGAGCGGC CGGCGTAAAG CCAACCCCCA AGACAAAGTC CACCCCATCA AGTCAGAGTT CATCAGGGCC AAGTACCAGA TGCTGGCATT TGTGCACAAG CTTCCCTGCC GGGACGATGA TGGAGTCACC GCCAAAGACC TCAGCAAGCA ACTACACTCG AGCGTGCGGA CAGGCAACCT GGAGACATGT CTGCGCCTGC TCTCCCTGGG TGCCCAGGCC AACTTCTTCC ACCCAGAGAA GGGCACCACA CCTCTGCACG TGGCTGCCAA GGCAGGACAG ACACTGCAGG CCGAGCTGCT TGTAGTGTAT GGGGCTGACC CTGGCTCCCC TGATGTTAAT GGCCGCACAC CCATTGACTA TGCCAGGCAG GCGGGGCACC ATGAGCTGGC GGAAAGGCTG GTTGAGTGCC AATATGAGCT CACTGACCGG CTGGCCTTCT ACCTCTGTGG ACGCAAGCCG GATCACAAGA ATGGGCATTA CATCATCCCA CAGATGGCTG ACAGATCTCG GCAAAAGTGC ATGTCTCAGA GCCTTGACTT ATCCGAATTG GCCAAAGCTG CTAAGAAGAA GCTGCAGGCG CTCAGCAACC GGCTTTTTGA GGAACTCGCC ATGGACGTGT ATGACGAGGT GGATCGAAGA GAAAATGATG CAGTGTGGCT GGCTACCCAA AACCACAGCA CTCTGGTGAC AGAGCGCAGT GCCGTGCCCT TCCTGCCTGT TAACCCGGAA TACTCAGCCA CGCGGAATCA GGGGCGACAA AAGCTGGCCC GCTTTAATGC CCGAGAGTTT GCCACCTTGA TCATCGACAT TCTCAGTGAG GCCAAGCGGA GACAGCAGGG CAAGAGCCTG AGCAGCCCCA CAGACAACCT CGAGCTGTCT CTGCGGAGCC AGAGTGACCT CGACGACCAA CACGACTACG ACAGCGTGGC CTCTGACGAG GACACAGACC AGGAGCCCCT GCGCAGCACC GGCGCCACTC GGAGCAACCG GGCCCGGAGC ATGGACTCCT CGGACTTGTC TGACGGGGCT GTGACGCTGC AGGAGTACCT GGAGCTGAAG AAGGCCCTGG CTACATCGGA GGCAAAGGTG CAGCAGCTCA TGAAGGTCAA CAGTAGCCTG AGCGACGAGC TCCGGAGGCT GCAGCGAGAG ATCCACAAGC TGCAGGCGGA GAACCTGCAG CTCCGGCAGC CTCCAGGGCC GGTGCCCACA CCTCCACTCC CCAGTGAACG GGCGGAACAC ACACCCATGG CGCCAGGCGG GAGCACACAC CGCAGGGATC GCCAGGCCTT TTCCATGTAT GAACCTGGCT CTGCCCTGAA GCCCTTTGGG GGCCCCCCTG GGGACGAGCT CACTACGCGG CTGCAGCCTT TCCACAGCAC TGAGCTAGAG GACGACGCCA TCTATTCAGT GCACGTCCCT GCTGGCCTTT ACCGGATCCG GAAAGGGGTG TCTGCCTCAG CTGTGCCCTT CACTCCCTCC TCCCCGCTGC TGTCCTGCTC CCAGGAGGGA AGCCGCCACA CGAGCAAGCT TTCCCGCCAC GGCAGTGGAG CCGACAGTGA CTATGAGAAC ACGCAAAGTG GGGACCCACT GCTGGGGCTG GAAGGGAAGA GGTTTCTAGA GCTGGGCAAA GAGGAAGACT TCCACCCAGA GCTGGAAAGC CTGGATGGAG ACCTAGATCC TGGGCTTCCC AGCACAGAGG ATGTCATCTT GAAGACAGAG CAGGTCACCA AGAACATTCA GGAACTGTTG CGGGCAGCCC AGGAGTTCAA GCATGACAGC TTCGTGCCCT GCTCAGAGAA GATCCATTTG GCTGTGACCG AGATGGCCTC CCTCTTCCCA AAGAGGCCAG CCCTGGAGCC AGTGCGGAGC TCACTGCGGC TGCTCAACGC CAGCGCCTAC CGGCTGCAGA GTGAGTGCCG GAAGACAGTG CCCCCAGAGC CCGGCGCCCC AGTGGACTTC CAGCTGCTGA CTCAGCAGGT GATCCAGTGC GCCTATGACA TCGCCAAGGC TGCCAAGCAG CTGGTCACCA TCACCACCCG AGAGAAGAAG CAGGGCGGTG GATCTGGCGA TTACAAGGAT GACGACGATA AGTGAGAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCC
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