pDS132
$128.00
In stock
SKU
PL76753
100μg liquid plasmid(Instantly):$163
| name | pDS132 | another name | apDS132 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | |
| Fragment species | Prokaryotic resistance | Chl | |
| Selective marker | SacB | Fluorescent labeling | |
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCATGTCAGC CGTTAAGTGT TCCTGTGTCA CTCAAAATTG CTTTGAGAGG CTCTAAGGGC TTCTCAGTGC GTTACATCCC TGGCTTGTTG TCCACAACCG TTAAACCTTA AAAGCTTTAA AAGCCTTATA TATTCTTTTT TTTCTTATAA AACTTAAAAC CTTAGAGGCT ATTTAAGTTG CTGATTTATA TTAATTTTAT TGTTCAAACA TGAGAGCTTA GTACGTGAAA CATGAGAGCT TAGTACGTTA GCCATGAGAG CTTAGTACGT TAGCCATGAG GGTTTAGTTC GTTAAACATG AGAGCTTAGT ACGTTAAACA TGAGAGCTTA GTACGTGAAA CATGAGAGCT TAGTACGTAC TATCAACAGG TTGAACTGCT GATCTTCAGA TCCTCTACGC CGGACGCATC GTGGCCGGAT CCAGCCGACC AGGCTTTCCA CGCCCGCGTG CCGCTCCATG TCGTTCGCGC GGTTCTCGGA AACGCGCTGC CGCGTTTCGT GATTGTCACG CTCAAGCCCG TAGTCCCGTT CGAGCGTCGC GCAGAGGTCA GCGAGGGCGC GGTAGGCCCG ATACGGCTCA TGGATGGTGT TTCGGGTCGG GTGAATCTTG TTGATGGCGA TATGGATGTG CAGGTTGTCG GTGTCGTGAT GCACGGCACT GACGCGCTGA TGCTCGGCGA AGCCAAGCCC AGCGCAGATG CGGTCCTCAA TCGCGCGCAA CGTCTCCGCG TCGGGCTTCT CTCCCGCGCG GAAGCTAACC AGCAGGTGAT AGGTCTTGTC GGCCTCGGAA CGGGTGTTGC CGTGCTGGGT CGCCATCACC TCGGCCATGA CAGCGGGCAG GGTGTTTGCC TCGCAGTTCG TGACGCGCAC GTGACCCAGG CGCTCGGTCT TGCCTTGCTC GTCGGTGATG TACTTCACCA GCTCCGCGAA GTCGCTCTTC TTGATGGAGC GCATGGGGAC GTGCTTGGCA ATCACGCGCA CCCCCCGGCC GTTTTAGCGG CTAAAAAAGT CATGGCTCTG CCCTCGGGCG GACCACGCCC ATCATGACCT TGCCAAGCTC GTCCTGCTTC TCTTCGATCT TCGCCAGCAG GGCGAGGATC GTGGCATCAC CGAACCGCGC CGTGCGCGGG TCGTCGGTGA GCCAGAGTTT CAGCAGGCCG CCCAGGCGGC CCAGGTCGCC ATTGATGCGG GCCAGCTCGC GGACGTGCTC ATAGTCCACG ACGCCCGTGA TTTTGTAGCC CTGGCCGACG GCCAGCAGGT AGGCCGACAG GCTCATGCCG GCCGCCGCCG CCTTTTCCTC AATCGCTCTT CGTTCGTCTG GAAGGCAGTA CACCTTGATA GGTGGGCTGC CCTTCCTGGT TGGCTTGGTT TCATCAGCCA TCCGCTTGCC CTCATCTGTT ACGCCGGCGG TAGCCGGCCA GCCTCGCAGA GCAGGATTCC CGTTGAGCAC CGCCAGGTGC GAATAAGGGA CAGTGAAGAA GGAACACCCG CTCGCGGGTG GGCCTACTTC ACCTATCCTG CCCGGCTGAC GCCGTTGGAT ACACCAAGGA AAGTCTACAC GAACCCTTTG GCAAAATCCT GTATATCGTG CGAAAAAGGA TGGATATACC GAAAAAATCG CTATAATGAC CCCGAAGCAG GGTTATGCAG CGGAAAAGCG CTGCTTCCCT GCTGTTTTGT GGAATATCTA CCGACTGGAA ACAGGCAAAT GCAGGAAATT ACTGAACTGA GGGGACAGGC GAGAGACGAT GCCAAAGAGC TACACCGACG AGCTGGCCGA GTGGGTTGAA TCCCGCGCGG CCAAGAAGCG CCGGCGTGAT GAGGCTGCGG TTGCGTTCCT GGCGGTGAGG GCGGATGTCG AGGCGGCGTT AGCGTCCGGC TATGCGCTCG TCACCATTTG GGAGCACATG CGGGAAACGG GGAAGGTCAA GTTCTCCTAC GAGACGTTCC GCTCGCACGC CAGGCGGCAC ATCAAGGCCA AGCCCGCCGA TGTGCCCGCA CCGCAGGCCA AGGCTGCGGA ACCCGCGCCG GCACCCAAGA CGCCGGAGCC ACGGCGGCCG AAGCAGGGGG GCAAGGCTGA AAAGCCGGCC CCCGCTGCGG CCCCGACCGG CTTCACCTTC AACCCAACAC CGGACAAAAA GGATCCTCTA CGCCGGACGC ATCGTGGCCG GCATCACCGG CGCCACAAGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA CCCGGGAATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC ACCAGCTCAC CGTCTTTCAT TGCCATACGG AATTCCGGAT GAGCATTCAT CAGGCGGGCA AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT TTCTCCATTT TAGCTTCCTT AGCTCCTGCC CTATGGGATT CACCTTTATG TTGATAAGAA ATAAAAGAAA ATGCCAATAG GATATCGGCA TTTTCTTTTG CGTTTTTATT TGTTAACTGT TAATTGTCCT TGTTCAAGGA TGCTGTCTTT GACAACAGAT GTTTTCTTGC CTTTGATGTT CAGCAGGAAG CTTGGCGCAA ACGTTGATTG TTTGTCTGCG TAGAATCCTC TGTTTGTCAT ATAGCTTGTA ATCACGACAT TGTTTCCTTT CGCTTGAGGT ACAGCGAAGT GTGAGTAAGT AAAGGTTACA TCGTTAGGAT CAAGATCCAT TTTTAACACA AGGCCAGTTT TGTTCAGCGG CTTGTATGGG CCAGTTAAAG AATTAGAAAC ATAACCAAGC ATGTAAATAT CGTTAGACGT AATGCCGTCA ATCGTCATTT TTGATCCGCG GGAGTCAGTG AACAGGTACC ATTTGCCGTT CATTTTAAAG ACGTTCGCGC GTTCAATTTC ATCTGTTACT GTGTTAGATG CAATCAGCGG TTTCATCACT TTTTTCAGTG TGTAATCATC GTTTAGCTCA ATCATACCGA GAGCGCCGTT TGCTAACTCA GCCGTGCGTT TTTTATCGCT TTGCAGAAGT TTTTGACTTT CTTGACGGAA GAATGATGTG CTTTTGCCAT AGTATGCTTT GTTAAATAAA GATTCTTCGC CTTGGTAGCC ATCTTCAGTT CCAGTGTTTG CTTCAAATAC TAAGTATTTG TGGCCTTTAT CTTCTACGTA GTGAGGATCT CTCAGCGTAT GGTTGTCGCC TGAGCTGTAG TTGCCTTCAT CGATGAACTG CTGTACATTT TGATACGTTT TTCCGTCACC GTCAAAGATT GATTTATAAT CCTCTACACC GTTGATGTTC AAAGAGCTGT CTGATGCTGA TACGTTAACT TGTGCAGTTG TCAGTGTTTG TTTGCCGTAA TGTTTACCGG AGAAATCAGT GTAGAATAAA CGGATTTTTC CGTCAGATGT AAATGTGGCT GAACCTGACC ATTCTTGTGT TTGGTCTTTT AGGATAGAAT CATTTGCATC GAATTTGTCG CTGTCTTTAA AGACGCGGCC AGCGTTTTTC CAGCTGTCAA TAGAAGTTTC GCCGACTTTT TGATAGAACA TGTAAATCGA TGTGTCATCC GCATTTTTAG GATCTCCGGC TAATGCAAAG ACGATGTGGT AGCCGTGATA GTTTGCGACA GTGCCGTCAG CGTTTTGTAA TGGCCAGCTG TCCCAAACGT CCAGGCCTTT TGCAGAAGAG ATATTTTTAA TTGTGGACGA ATCGAATTCA GGAACTTGAT ATTTTTCATT TTTTTGCTGT TCAGGGATTT GCAGCATATC ATGGCGTGTA ATATGGGAAA TGCCGTATGT TTCCTTATAT GGCTTTTGGT TCGTTTCTTT CGCAAACGCT TGAGTTGCGC CTCCTGCCAG CAGTGCGGTA GTAAAGGTTA ATACTGTTGC TTGTTTTGCA AACTTTTTGA TGTTCATCGT TCATGTCTCC TTTTTTATGT ACTGTGTTAG CGGTCTGCTT CTTCCAGCCC TCCTGTTTGA AGATGGCAAG TTAGTTACGC ACAATAAAAA AAGACCTAAA ATATGTAAGG GGTGACGCCA AAGTATACAC TTTGCCCTTT ACACATTTTA GGTCTTGCCT GCTTTATCAG TAACAAACCC GCGCGATTTA CTTTTCGACC TCATTCTATT AGACTCTCGT TTGGATTGCA ACTGGTCTAT TTTCCTCTTT TGTTTGATAG AAAATCATAA AAGGATTTGC AGACTACGGG CCTAAAGAAC TAAAAAATCT ATCTGTTTCT TTTCATTCTC TGTATTTTTT ATAGTTTCTG TTGCATGGGC ATAAAGTTGC CTTTTTAATC ACAATTCAGA AAATATCATA ATATCTCATT TCACTAAATA ATAGTGAACG GCAGGTATAT GTGATGGGTT AAAAAGGATC GATCCTCTAG AGTCGACCTG CAGGTCGACG GATCTGCAGG TCGACGGATC CCAAGCTTCT TCTAGAGGTA CCGCATGCGA TATCGAGCTC TCCCGGGAAT TCCACAAATT GTTATCCGCT CACAATTCCA CATGTGGAAT TCCACATGTG GAATTC
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