pCW57 MCS_P2A_EBF1(RFP)
$84.00
In stock
SKU
PL76030
NM_001290360.2;COE1;EBF;O/E-1;OLF1
100μg liquid plasmid(Instantly):$105
| name | pCW57 MCS_P2A_EBF1(RFP) | another name | apCW57 MCS_P2A_EBF1(RFP) |
| Insert Size (bp) | 1779bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TRE | Replicon | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCTCGAC CTCGAGACAA ATGGCAGTAT TCATCCACAA TTTTAAAAGA AAAGGGGGGA TTGGGGGGTA CAGTGCAGGG GAAAGAATAG TAGACATAAT AGCAACAGAC ATACAAACTA AAGAATTACA AAAACAAATT ACAAAAATTC AAAATTTTCG GGTTTATTAC AGGGACAGCA GAGATCCACT TTGGCCGCGA ATCGATATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GTATGTCGAG TTTACTCCCT ATCAGTGATA GAGAACGATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ACTCCCTATC AGTGATAGAG AACGTATGTC GAGTTTATCC CTATCAGTGA TAGAGAACGT ATGTCGAGTT TACTCCCTAT CAGTGATAGA GAACGTATGT CGAGGTAGGC GTGTACGGTG GGAGGCCTAT ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GAATTGGCTA GCGAATTCGT CGACACCGGT TCCGGAGCCA CGAACTTCTC TCTGTTAAAG CAAGCAGGAG ACGTGGAAGA AAACCCCGGT CCTAGGCTGC AGACGCGTAT GTTTGGGATT CAGGAAAGCA TCCAACGGAG TGGAAGCAGC ATGAAGGAAG AGCCGCTGGG CAGCGGCATG AACGCGGTGC GGACGTGGAT GCAGGGCGCC GGGGTGCTGG ACGCCAACAC GGCGGCGCAG AGCGGGGTGG GTCTGGCCCG GGCTCACTTT GAGAAGCAGC CGCCTTCCAA TCTGCGGAAA TCCAACTTCT TCCACTTCGT CCTGGCCCTC TACGACAGAC AGGGCCAGCC CGTGGAGATC GAGAGGACAG CGTTTGTGGG GTTCGTGGAG AAGGAAAAAG AAGCCAACAG CGAAAAGACC AATAACGGAA TTCACTACCG GCTTCAGCTT CTCTACAGCA ATGGGATAAG GACGGAGCAG GATTTCTACG TGCGCCTCAT TGACTCCATG ACAAAACAAG CCATAGTGTA TGAAGGCCAA GACAAGAACC CAGAAATGTG CCGAGTCTTG CTCACACATG AGATCATGTG CAGCCGCTGT TGTGACAAGA AAAGCTGTGG CAACCGAAAT GAGACTCCCT CAGATCCAGT GATAATTGAC AGGTTCTTCT TGAAATTTTT CCTCAAATGT AACCAAAATT GCCTAAAGAA TGCGGGAAAC CCACGTGACA TGCGGAGATT CCAGGTCGTG GTGTCTACGA CAGTCAATGT GGATGGCCAT GTCCTGGCAG TCTCTGATAA CATGTTTGTC CATAATAATT CCAAGCATGG GCGGAGGGCT CGGAGGCTTG ACCCCTCGGA AGGTACGCCC TCTTATCTGG AACATGCAGC TACTCCCTGT ATCAAAGCCA TCAGCCCGAG TGAAGGATGG ACGACGGGAG GTGCGACTGT GATCATCATA GGGGACAATT TCTTTGATGG GTTACAGGTC ATATTCGGTA CCATGCTGGT CTGGAGTGAG TTGATCACTC CTCATGCCAT CCGTGTGCAG ACCCCTCCTC GGCACATCCC TGGTGTTGTG GAAGTCACAC TGTCCTACAA ATCTAAGCAG TTCTGCAAAG GAACACCAGG CAGATTCATT TATACAGCGC TCAACGAACC CACCATCGAT TATGGTTTCC AGAGGTTACA GAAGGTCATT CCTCGGCACC CTGGTGACCC TGAGCGTTTG CCAAAGGAAG TAATACTCAA AAGGGCTGCG GATCTGGTAG AAGCACTGTA TGGGATGCCA CACAACAACC AGGAAATCAT TCTGAAGAGA GCGGCCGACA TTGCCGAGGC CCTGTACAGT GTTCCCCGCA ACCACAACCA ACTCCCGGCC CTTGCTAACA CCTCGGTCCA CGCAGGGATG ATGGGCGTGA ATTCGTTCAG TGGACAACTG GCCGTGAATG TCTCCGAGGC ATCACAAGCC ACCAATCAGG GTTTCACCCG CAACTCAAGC AGCGTATCAC CACACGGGTA CGTGCCGAGC ACCACTCCCC AGCAGACCAA CTATAACTCC GTCACCACGA GCATGAACGG ATACGGCTCT GCCGCAATGT CCAATTTGGG CGGCTCCCCC ACCTTCCTCA ACGGCTCAGC TGCCAACTCC CCCTATGCCA TAGTGCCATC CAGCCCCACC ATGGCCTCCT CCACAAGCCT CCCCTCCAAC TGCAGCAGCT CCTCGGGCAT CTTCTCCTTC TCACCAGCCA ACATGGTCTC AGCCGTGAAA CAGAAGAGTG CTTTCGCACC AGTCGTCAGA CCCCAGACCT CCCCACCTCC CACCTGCACC AGCACCAACG GGAACAGCCT GCAAGCGATA TCTGGCATGA TTGTTCCTCC TATGTGAGGA TCCGGGGTTG GGGTTGCGCC TTTTCCAAGG CAGCCCTGGG TTTGCGCAGG GACGCGGCTG CTCTGGGCGT GGTTCCGGGA AACGCAGCGG CGCCGACCCT GGGTCTCGCA CATTCTTCAC GTCCGTTCGC AGCGTCACCC GGATCTTCGC CGCTACCCTT GTGGGCCCCC CGGCGACGCT TCCTGCTCCG CCCCTAAGTC GGGAAGGTTC CTTGCGGTTC GCGGCGTGCC GGACGTGACA AACGGAAGCC GCACGTCTCA CTAGTACCCT CGCAGACGGA CAGCGCCAGG GAGCAATGGC AGCGCGCCGA CCGCGATGGG CTGTGGCCAA TAGCGGCTGC TCAGCAGGGC GCGCCGAGAG CAGCGGCCGG GAAGGGGCGG TGCGGGAGGC GGGGTGTGGG GCGGTAGTGT GGGCCCTGTT CCTGCCCGCG CGGTGTTCCG CATTCTGCAA GCCTCCGGAG CGCACGTCGG CAGTCGGCTC CCTCGTTGAC CGAATCACCG ACCTCTCTCC CCAGCAATTC ACCCGCGCGC GGCCACCATG AGCGAGCTGA TCAAGGAGAA CATGCACATG AAGCTGTACA TGGAGGGCAC CGTGAACAAC CACCACTTCA AGTGCACATC CGAGGGCGAA GGCAAGCCCT ACGAGGGCAC CCAGACCATG AAGATCAAGG TGGTCGAGGG CGGCCCTCTC CCCTTCGCCT TCGACATCCT GGCTACCAGC TTCATGTACG GCAGCAAAGC CTTCATCAAC CACACCCAGG GCATCCCCGA CTTCTTTAAG CAGTCCTTCC CTGAGGGCTT CACATGGGAG AGAATCACCA CATACGAAGA CGGGGGCGTG CTGACCGCTA CCCAGGACAC CAGCTTCCAG AACGGCTGCA TCATCTACAA CGTCAAGATC AACGGGGTGA ACTTCCCATC CAACGGCCCT GTGATGCAGA AGAAAACACG CGGCTGGGAG GCCAACACCG AGATGCTGTA CCCCGCTGAC GGCGGCCTGA GAGGCCACAG CCAGATGGCC CTGAAGCTCG TGGGCGGGGG CTACCTGCAC TGCTCCTTCA AGACCACATA CAGATCCAAG AAACCCGCTA AGAACCTCAA GATGCCCGGC TTCCACTTCG TGGACCACAG ACTGGAAAGA ATCAAGGAGG CCGACAAAGA GACCTACGTC GAGCAGCACG AGATGGCTGT GGCCAAGTAC TGCGACCTCC CTAGCAAACT GGGGCACAGA TTCGAAGGAA GCGGAGAAGG TAGAGGTTCT CTCCTCACTT GTGGTGATGT TGAAGAAAAC CCTGGTCCAA TGTCTAGACT GGACAAGAGC AAAGTCATAA ACGGAGCTCT GGAATTACTC AATGGTGTCG GTATCGAAGG CCTGACGACA AGGAAACTCG CTCAAAAGCT GGGAGTTGAG CAGCCTACCC TGTACTGGCA CGTGAAGAAC AAGCGGGCCC TGCTCGATGC CCTGCCAATC GAGATGCTGG ACAGGCATCA TACCCACTTC TGCCCCCTGG AAGGCGAGTC ATGGCAAGAC TTTCTGCGGA ACAACGCCAA GTCATACCGC TGTGCTCTCC TCTCACATCG CGACGGGGCT AAAGTGCATC TCGGCACCCG CCCAACAGAG AAACAGTACG AAACCCTGGA AAATCAGCTC GCGTTCCTGT GTCAGCAAGG CTTCTCCCTG GAGAACGCAC TGTACGCTCT GTCCGCCGTG GGCCACTTTA CACTGGGCTG CGTATTGGAG GAACAGGAGC ATCAAGTAGC AAAAGAGGAA AGAGAGACAC CTACCACCGA TTCTATGCCC CCACTTCTGA GACAAGCAAT TGAGCTGTTC GACCGGCAGG GAGCCGAACC TGCCTTCCTT TTCGGCCTGG AACTAATCAT ATGTGGCCTG GAGAAACAGC TAAAGTGCGA AAGCGGCGGG CCGACCGACG CCCTTGACGA TTTTGACTTA GACATGCTCC CAGCCGATGC CCTTGACGAC TTTGACCTTG ATATGCTGCC TGCTGACGCT CTTGACGATT TTGACCTTGA CATGCTCCCC GGGTAACTAA GTAAGGATCG ATCCAAGATA TCGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG CCTGTTTCGC CTCGGCGTCC GGACTAGAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT AGCTATCCCG CCCCTAACTC CGCCCATCCC GCCCCTAACT CCGCCCAGTT CCGCCCATTC TCCGCCCCAT GGCTGACTAA TTTTTTTTAT TTATGCAGAG GCCGAGGCCG CCTCGGCCTC TGAGCTATTC CAGAAGTAGT GAGGAGGCTT TTTTGGAGGC CTTTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC GCGCAATTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT GCAAGCTTAA TGTAGTCTTA TGCAATACTC TTGTAGTCTT GCAACATGGT AACGATGAGT TAGCAACATG CCTTACAAGG AGAGAAAAAG CACCGTGCAT GCCGATTGGT GGAAGTAAGG TGGTACGATC GTGCCTTATT AGGAAGGCAA CAGACGGGTC TGACATGGAT TGGACGAACC ACTGAATTGC CGCATTGCAG AGATATTGTA TTTAAGTGCC TAGCTCGATA CATAAACGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT GGCGCCCGAA CAGGGACTTG AAAGCGAAAG GGAAACCAGA GGAGCTCTCT CGACGCAGGA CTCGGCTTGC TGAAGCGCGC ACGGCAAGAG GCGAGGGGCG GCGACTGGTG AGTACGCCAA AAATTTTGAC TAGCGGAGGC TAGAAGGAGA GAGATGGGTG CGAGAGCGTC AGTATTAAGC GGGGGAGAAT TAGATCGCGA TGGGAAAAAA TTCGGTTAAG GCCAGGGGGA AAGAAAAAAT ATAAATTAAA ACATATAGTA TGGGCAAGCA GGGAGCTAGA ACGATTCGCA GTTAATCCTG GCCTGTTAGA AACATCAGAA GGCTGTAGAC AAATACTGGG ACAGCTACAA CCATCCCTTC AGACAGGATC AGAAGAACTT AGATCATTAT ATAATACAGT AGCAACCCTC TATTGTGTGC ATCAAAGGAT AGAGATAAAA GACACCAAGG AAGCTTTAGA CAAGATAGAG GAAGAGCAAA ACAAAAGTAA GACCACCGCA CAGCAAGCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGAC
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