pCAGGS-FLAG-DDX58
$84.00
In stock
SKU
PL75990
NM_014314.4;RIG-I;RIG1;RIGI;RLR-1;SGMRT2
100μg liquid plasmid(Instantly):$105
| name | pCAGGS-FLAG-DDX58 | another name | apCAGGS-FLAG-DDX58 |
| Insert Size (bp) | 2778bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CAG | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | pcaggs-F：GTTCGGCTTCTGGCGTGT | |
| 3′ sequencing primer | pcaggs-R：TATGTCCTTCCGAGTGAGAG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGGAT TACAAGGATG ACGACGATAA GGAATTCGAG CTCATCGATG GTACCATGAC CACCGAGCAG CGACGCAGCC TGCAAGCCTT CCAGGATTAT ATCCGGAAGA CCCTGGACCC TACCTACATC CTGAGCTACA TGGCCCCCTG GTTTAGGGAG GAAGAGGTGC AGTATATTCA GGCTGAGAAA AACAACAAGG GCCCAATGGA GGCTGCCACA CTTTTTCTCA AGTTCCTGTT GGAGCTCCAG GAGGAAGGCT GGTTCCGTGG CTTTTTGGAT GCCCTAGACC ATGCAGGTTA TTCTGGACTT TATGAAGCCA TTGAAAGTTG GGATTTCAAA AAAATTGAAA AGTTGGAGGA GTATAGATTA CTTTTAAAAC GTTTACAACC AGAATTTAAA ACCAGAATTA TCCCAACCGA TATCATTTCT GATCTGTCTG AATGTTTAAT TAATCAGGAA TGTGAAGAAA TTCTACAGAT TTGCTCTACT AAGGGGATGA TGGCAGGTGC AGAGAAATTG GTGGAATGCC TTCTCAGATC AGACAAGGAA AACTGGCCCA AAACTTTGAA ACTTGCTTTG GAGAAAGAAA GGAACAAGTT CAGTGAACTG TGGATTGTAG AGAAAGGTAT AAAAGATGTT GAAACAGAAG ATCTTGAGGA TAAGATGGAA ACTTCTGACA TACAGATTTT CTACCAAGAA GATCCAGAAT GCCAGAATCT TAGTGAGAAT TCATGTCCAC CTTCAGAAGT GTCTGATACA AACTTGTACA GCCCATTTAA ACCAAGAAAT TACCAATTAG AGCTTGCTTT GCCTGCTATG AAAGGAAAAA ACACAATAAT ATGTGCTCCT ACAGGTTGTG GAAAAACCTT TGTTTCACTG CTTATATGTG AACATCATCT TAAAAAATTC CCACAAGGAC AAAAGGGGAA AGTTGTCTTT TTTGCGAATC AGATCCCAGT GTATGAACAG CAGAAATCTG TATTCTCAAA ATACTTTGAA AGACATGGGT ATAGAGTTAC AGGCATTTCT GGAGCAACAG CTGAGAATGT CCCAGTGGAA CAGATTGTTG AGAACAATGA CATCATCATT TTAACTCCAC AGATTCTTGT GAACAACCTT AAAAAGGGAA CGATTCCATC ACTATCCATC TTTACTTTGA TGATATTTGA TGAATGCCAC AACACTAGTA AACAACACCC GTACAATATG ATCATGTTTA ATTATCTAGA TCAGAAACTT GGAGGATCTT CAGGCCCACT GCCCCAGGTC ATTGGGCTGA CTGCCTCGGT TGGTGTTGGG GATGCCAAAA ACACAGATGA AGCCTTGGAT TATATCTGCA AGCTGTGTGC TTCTCTTGAT GCGTCAGTGA TAGCAACAGT CAAACACAAT CTGGAGGAAC TGGAGCAAGT TGTTTATAAG CCCCAGAAGT TTTTCAGGAA AGTGGAATCA CGGATTAGCG ACAAATTTAA ATACATCATA GCTCAGCTGA TGAGGGACAC AGAGAGTCTG GCAAAGAGAA TCTGCAAAGA CCTCGAAAAC TTATCTCAAA TTCAAAATAG GGAATTTGGA ACACAGAAAT ATGAACAATG GATTGTTACA GTTCAGAAAG CATGCATGGT GTTCCAGATG CCAGACAAAG ATGAAGAGAG CAGGATTTGT AAAGCCCTGT TTTTATACAC TTCACATTTG CGGAAATATA ATGATGCCCT CATTATCAGT GAGCATGCAC GAATGAAAGA TGCTCTGGAT TACTTGAAAG ACTTCTTCAG CAATGTCCGA GCAGCAGGAT TCGATGAGAT TGAGCAAGAT CTTACTCAGA GATTTGAAGA AAAGCTGCAG GAACTAGAAA GTGTTTCCAG GGATCCCAGC AATGAGAATC CTAAACTTGA AGACCTCTGC TTCATCTTAC AAGAAGAGTA CCACTTAAAC CCAGAGACAA TAACAATTCT CTTTGTGAAA ACCAGAGCAC TTGTGGACGC TTTAAAAAAT TGGATTGAAG GAAATCCTAA ACTCAGTTTT CTAAAACCTG GCATATTGAC TGGACGTGGC AAAACAAATC AGAACACAGG AATGACCCTC CCGGCACAGA AGTGTATATT GGATGCATTC AAAGCCAGTG GAGATCACAA TATTCTGATT GCCACCTCAG TTGCTGATGA AGGCATTGAC ATTGCACAGT GCAATCTTGT CATCCTTTAT GAGTATGTGG GCAATGTCAT CAAAATGATC CAAACCAGAG GCAGAGGAAG AGCAAGAGGT AGCAAGTGCT TCCTTCTGAC TAGTAATGCT GGTGTAATTG AAAAAGAACA AATAAACATG TACAAAGAAA AAATGATGAA TGACTCTATT TTACGCCTTC AGACATGGGA CGAAGCAGTA TTTAGGGAAA AGATTCTGCA TATACAGACT CATGAAAAAT TCATCAGAGA TAGTCAAGAA AAACCAAAAC CTGTACCTGA TAAGGAAAAT AAAAAACTGC TCTGCAGAAA GTGCAAAGCC TTGGCATGTT ACACAGCTGA CGTAAGAGTG ATAGAGGAAT GCCATTACAC TGTGCTTGGA GATGCTTTTA AGGAATGCTT TGTGAGTAGA CCACATCCCA AGCCAAAGCA GTTTTCAAGT TTTGAAAAAA GAGCAAAGAT ATTCTGTGCC CGACAGAACT GCAGCCATGA CTGGGGAATC CATGTGAAGT ACAAGACATT TGAGATTCCA GTTATAAAAA TTGAAAGTTT TGTGGTGGAG GATATTGCAA CTGGAGTTCA GACACTGTAC TCGAAGTGGA AGGACTTTCA TTTTGAGAAG ATACCATTTG ATCCAGCAGA AATGTCCAAA TGACTCGAGC TAGCAGATCT TAATTAATTA AGGATCTTTT TCCCTCTGCC AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA GAGGTCATCA GTATATGAAA CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC TTATGGAGAT CCCTCGACCT GCAGCCCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCGGATCCGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCCGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGG
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