pBE2R-mCitrine
$84.00
In stock
SKU
PL75809
100μg liquid plasmid(Instantly):$120
| name | pBE2R-mCitrine | another name | apBE2R-mCitrine |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Kan | Fluorescent labeling | Yellow |
| Promoter | p43 | Replicon | repB |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATTAATAA TAGATTTTAG CTTTTTATTT GTTGAAAAAA GCTAATCAAA TTGTTGTCGG GATCAATTAC TGCAAAGTCT CGTTCATCCC ACCACTGATC TTTTAATGAT GTATTGGGGT GCAAAATGCC CAAAGGCTTA ATATGTTGAT ATAATTCATC AATTCCCTCT ACTTCAATGC GGCAACTAGC AGTACCAGCA ATAAACGACT CCGCACCTGT ACAAACCGGT GAATCATTAC TACGAGAGCG CCAGCTTCAT CACTTGCCTC CCATAGATGA ATCCGAACCT CATTACACAT TAGAACTGCG AATCCATCTT CATGGTGAAC CAAAGTGAAA CCTAGTTTAT CGCAATAAAA ACCTATACTC TTTTTAATAT CCCCGACTGG CAATGCCGGG ATAGACTGTA ACATTCTCAC GCATAAAATC CCCTTTCATT TTCTAATGTA AATCTATTAC CTTATTATTA ATTCAATTCG CTCATAATTA ATCCTTTTTC TTATTACGCA AAATGGCCCG ATTTAAGCAC ACCCTTTATT CCGTTAATGC GCCATGACAG CCATGATAAT TACTAATACT AGGAGAAGTT AATAAATACG TAACCAACAT GATTAACAAT TATTAGAGGT CATCGTTCAA AATGGTATGC GTTTTGACAC ATCCACTATA TATCCGTGTC GTTCTGTCCA CTCCTGAATC CCATTCCAGA AATTCTCTAG CGATTCCAGA AGTTTCTCAG AGTCGGAAAG TTGACCAGAC ATTACGAACT GGCACAGATG GTCATAACCT GAAGGAAGAT CTGATTGCTT AACTGCTTCA GTTAAGACCG AAGCGCTCGT CGTATAACAG ATGCGATGAT GCAGACCAAT CAACATGGCA CCTGCCATTG CTACCTGTAC AGTCAAGGAT GGTAGAAATG TTGTCGGTCC TTGCACACGA ATATTACGCC ATTTGCCTGC ATATTCAAAC AGCTCTTCTA CGATAAGGGC ACAAATCGCA TCGTGGAACG TTTGGGCTTC TACCGATTTA GCAGTTTGAT ACACTTTCTC TAAGTATCCA CCTGAATCAT AAATCGGCAA AATAGAGAAA AATTGACCAT GTGTAAGCGG CCAATCTGAT TCCACCTGAG ATGCATAATC TAGTAGAATC TCTTCGCTAT CAAAATTCAC TTCCACCTTC CACTCACCGG TTGTCCATTC ATGGCTGAAC TCTGCTTCCT CTGTTGACAT GACACACATC ATCTCAATAT CCGAATAGGG CCCATCAGTC TGACGACCAA GAGAGCCATA AACACCAATA GCCTTAACAT CATCCCCATA TTTATCCAAT ATTCGTTCCT TAATTTCATG AACAATCTTC ATTCTTTCTT CTCTAGTCAT TATTATTGGT CCATTCACTA TTCTCATTCC CTTTTCAGAT AATTTTAGAT TTGCTTTTCT AAATAAGAAT ATTTGGAGAG CACCGTTCTT ATTCAGCTAT TAATAACTCG TCTTCCTAAG CATCCTTCAA TCCTTTTAAT AACAATTATA GCATCTAATC TTCAACAAAC TGGCCCGTTT GTTGAACTAC TCTTTAATAA AATAATTTTT CCGTTCCCAA TTCCACATTG CAATAATAGA AAATCCATCT TCATCGGCTT TTTCGTCATC ATCTGTATGA ATCAAATCGC CTTCTTCTGT GTCATCAAGG TTTAATTTTT TATGTATTTC TTTTAACAAA CCACCATAGG AGATTAACCT TTTACGGTGT AAACCTTCCT CCAAATCAGA CAAACGTTTC AAATTCTTTT CTTCATCATC GGTCATAAAA TCCGTATCCT TTACAGGATA TTTTGCAGTT TCGTCAATTG CCGATTGTAT ATCCGATTTA TATTTATTTT TCGGTCGAAT CATTTGAACT TTTACATTTG GATCATAGTC TAATTTCATT GCCTTTTTCC AAAATTGAAT CCATTGTTTT TGATTCACGT AGTTTTCTGT ATTCTTAAAA TAAGTTGGTT CCACACATAC CAATACATGC ATGTGCTGAT TATAAGAATT ATCTTTATTA TTTATTGTCA CTTCCGTTGC ACGCATAAAA CCAACAAGAT TTTTATTAAT TTTTTTATAT TGCATCATTC GGCGAAATCC TTGAGCCATA TCTGACAAAC TCTTATTTAA TTCTTCGCCA TCATAAACAT TTTTAACTGT TAATGTGAGA AACAACCAAC GAACTGTTGG CTTTTGTTTA ATAACTTCAG CAACAACCTT TTGTGACTGA ATGCCATGTT TCATTGCTCT CCTCCAGTTG CACATTGGAC AAAGCCTGGA TTTACAAAAC CACACTCGAT ACAACTTTCT TTCGCCTGTT TCACGATTTT GTTTATACTC TAATATTTCA GCACAATCTT TTACTCTTTC AGCCTTTTTA AATTCAAGAA TATGCAGAAG TTCAAAGTAA TCAACATTAG CGATTTTCTT TTCTCTCCAT GGTCTCACTT TTCCACTTTT TGTCTTGTCC ACTAAAACCC TTGATTTTTC ATCTGAATAA ATGCTACTAT TAGGACACAT AATATTAAAA GAAACCCCCA TCTATTTAGT TATTTGTTTA GTCACTTATA ACTTTAACAG ATGGGGTTTT TCTGTGCAAC CAATTTTAAG GGTTTTCAAT ACTTTAAAAC ACATACATAC CAACACTTCA ACGCACCTTT CAGCAACTAA AATAAAAATG ACGTTATTTC TATATGTATC AAGATAAGAA AGAACAAGTT CAAAACCATC AAAAAAAGAC ACCTTTTCAG GTGCTTTTTT TATTTTATAA ACTCATTCCC TGATCTCGAC TTCGTTCTTT TTTTACCTCT CGGTTATGAG TTAGTTCAAA TTCGTTCTTT TTAGGTTCTA AATCGTGTTT TTCTTGGAAT TGTGCTGTTT TATCCTTTAC CTTGTCTACA AACCCCTTAA AAACGTTTTT AAAGGCTTTT AAGCCGTCTG TACGTTCCTT AAGGAATTCG AGCTCAGCAT TATTGAGTGG ATGATTATAT TCCTTTTGAT AGGTGGTATG TTTTCGCTTG AACTTTTAAA TACAGCCATT GAACATACGG TTGATTTAAT AACTGACAAA CATCACCCTC TTGCTAAAGC GGCCAAGGAC GCTGCCGCCG GGGCTGTTTG CGTTTTTGCC GTGATTTCGT GTATTATTGG TTTACTTATT TTTTTGCCAA AGCTGTAATG GCTGAAAATT CTTACATTTA TTTTACATTT TTAGAAATGG GCGTGAAAAA AAGCGCGCGA TTATGTAAAA TATAAAGTGA TAGCGGTACA AGAGAGGAAT GTACACATGA GCAAAGGCGA AGAACTGTTT ACCGGTGTTG TTCCGATTCT GGTTGAACTG GATGGTGATG TTAATGGCCA CAAATTTTCA GTTAGCGGTG AAGGCGAAGG TGATGCAACC TATGGTAAAC TGACCCTGAA ATTTATCTGT ACCACCGGCA AACTGCCGGT TCCGTGGCCG ACACTGGTTA CCACCTTTGG TTATGGTCTG ATGTGTTTTG CACGTTATCC GGATCATATG AAACAGCACG ATTTTTTCAA AAGCGCAATG CCGGAAGGTT ATGTTCAAGA ACGTACCATC TTCTTCAAAG ATGACGGCAA CTATAAAACC CGTGCCGAAG TTAAATTTGA AGGTGATACC CTGGTGAATC GCATTGAACT GAAAGGCATC GATTTTAAAG AGGATGGTAA TATCCTGGGC CACAAACTGG AATATAATTA TAATAGCCAC AACGTGTACA TCATGGCCGA CAAACAGAAA AATGGCATCA AAGTGAACTT CAAGATCCGC CATAATATTG AAGATGGTTC AGTTCAGCTG GCCGATCATT ATCAGCAGAA TACCCCGATT GGTGATGGTC CGGTTCTGCT GCCGGATAAT CATTATCTGA GCTATCAGAG CAAACTGAGC AAAGATCCGA ATGAAAAACG TGATCACATG GTGCTGCTGG AATTTGTTAC CGCAGCAGGT ATTACCTTAG GTATGGATGA ACTGTACAAA TAATGTATTC TATAGTGTCA CCTAAATCGT ATGTGTATGA TACATAAGGT TATGTATTAA TTGTAGCCGC GTTCTAACGA CAATATGTAC AAGCCTAATT GTGTAGCATC TGGCTTACTG AAGCAGACCC TATCATCTCT CTCGTAAACT GCCGTCAGAG TCGGTTTGGT TGGACGAACC TTCTGAGTTT CTGGTAACGC CGTCCCGCAC CCGGAAATGG TCAGCGAACC AATCAGCAGG GTCATCGCTA GCCAGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCACCATTCC TTGCGGCGGC GGTGCTCAAC GGCCTCAACC TACTACTGGG CTGCTTCCTA ATGCAGGAGT CGCATAAGGG AGAGCGTCGA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGATTTC ACTTTTTGCA TTCTACAAAC TGCATAACTC ATATGTAAAT CGCTCCTTTT TAGGTGGCAC AAATGTGAGG CATTTTCGCT CTTTCCGGCA ACCACTTCCA AGTAAAGTAT AACACACTAT ACTTTATATT CATAAAGTGT GTGCTCTGCG AGGCTGTCGG CAGTGCCGAC CAAAACCATA AAACCTTTAA GACCTTTCTT TTTTTTACGA GAAAAAAGAA ACAAAAAAAC CTGCCCTCTG CCACCTCAGC AAAGGGGGGT TTTGCTCTCG TGCTCGTTTA AAAATCAGCA AGGGACAGGT AGTATTTTTT GAGAAGATCA CTCAAAAAAT CTCCACCTTT AAACCCTTGC CAATTTTTAT TTTGTCCGTT TTGTCTAGCT TACCGAAAGC CAGACTCAGC AAGAATAAAA TTTTTATTGT CTTTCGGTTT TCTAGTGTAA CGGACAAAAC CACTCAAAAT AAAAAAGATA CAAGAGAGGT CTCTCGTATC TTTTATTCAG CAATCGCGCC CGATTGCTGA AC
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