pBE2R(cm)
$84.00
In stock
SKU
PL75801
100μg liquid plasmid(Instantly):$120
| name | pBE2R(cm) | another name | apBE2R(cm) |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | p43 | Replicon | repB |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGAT TTCACTTTTT GCATTCTACA AACTGCATAA CTCATATGTA AATCGCTCCT TTTTAGGTGG CACAAATGTG AGGCATTTTC GCTCTTTCCG GCAACCACTT CCAAGTAAAG TATAACACAC TATACTTTAT ATTCATAAAG TGTGTGCTCT GCGAGGCTGT CGGCAGTGCC GACCAAAACC ATAAAACCTT TAAGACCTTT CTTTTTTTTA CGAGAAAAAA GAAACAAAAA AACCTGCCCT CTGCCACCTC AGCAAAGGGG GGTTTTGCTC TCGTGCTCGT TTAAAAATCA GCAAGGGACA GGTAGTATTT TTTGAGAAGA TCACTCAAAA AATCTCCACC TTTAAACCCT TGCCAATTTT TATTTTGTCC GTTTTGTCTA GCTTACCGAA AGCCAGACTC AGCAAGAATA AAATTTTTAT TGTCTTTCGG TTTTCTAGTG TAACGGACAA AACCACTCAA AATAAAAAAG ATACAAGAGA GGTCTCTCGT ATCTTTTATT CAGCAATCGC GCCCGATTGC TGAACAGATT AATAATAGAT TTTAGCTTTT TATTTGTTGA AAAAAGCTAA TCAAATTGTT GTCGGGATCA ATTACTGCAA AGTCTCGTTC ATCCCACCAC TGATCTTTTA ATGATGTATT GGGGTGCAAA ATGCCCAAAG GCTTAATATG TTGATATAAT TCATCAATTC CCTCTACTTC AATGCGGCAA CTAGCAGTAC CAGCAATAAA CGACTCCGCA CCTGTACAAA CCGGTGAATC ATTACTACGA GAGCGCCAGC TTCATCACTT GCCTCCCATA GATGAATCCG AACCTCATTA CACATTAGAA CTGCGAATCC ATCTTCATGG TGAACCAAAG TGAAACCTAG TTTATCGCAA TAAAAACCTA TACTCTTTTT AATATCCCCG ACTGGCAATG CCGGGATAGA CTGTAACATT CTCACGCATA AAATCCCCTT TCATTTTCTA ATGTAAATCT ATTACCTTAT TATTAATTCA ATTCGCTCAT AATTAATCCT TTTTCTTATT ACGCAAAATG GCCCGATTTA AGCACACCCT TTATTCCGTT AATGCGCCAT GACAGCCATG ATAATTACTA ATACTAGGAG AAGTTAATAA ATACGTAACC AACATGATTA ACAATTATTA GAGGTCATCG TTCAAAATGG TATGCGTTTT GACACATCCA CTATATATCC GTGTCGTTCT GTCCACTCCT GAATCCCATT CCAGAAATTC TCTAGCGATT CCAGAAGTTT CTCAGAGTCG GAAAGTTGAC CAGACATTAC GAACTGGCAC AGATGGTCAT AACCTGAAGG AAGATCTGAT TGCTTAACTG CTTCAGTTAA GACCGAAGCG CTCGTCGTAT AACAGATGCG ATGATGCAGA CCAATCAACA TGGCACCTGC CATTGCTACC TGTACAGTCA AGGATGGTAG AAATGTTGTC GGTCCTTGCA CACGAATATT ACGCCATTTG CCTGCATATT CAAACAGCTC TTCTACGATA AGGGCACAAA TCGCATCGTG GAACGTTTGG GCTTCTACCG ATTTAGCAGT TTGATACACT TTCTCTAAGT ATCCACCTGA ATCATAAATC GGCAAAATAG AGAAAAATTG ACCATGTGTA AGCGGCCAAT CTGATTCCAC CTGAGATGCA TAATCTAGTA GAATCTCTTC GCTATCAAAA TTCACTTCCA CCTTCCACTC ACCGGTTGTC CATTCATGGC TGAACTCTGC TTCCTCTGTT GACATGACAC ACATCATCTC AATATCCGAA TAGGGCCACA GTCGGCATTA TCTCATATTA TAAAAGCCAG TCATTAGGCC TATCTGACAA TTCCTGAATA GAGTTCATAA ACAATCCTGC ATGATAACCA TCACAAACAG AATGATGTAC CTGTAAAGAT AGCGGTAAAT ATATTGAATT ACCTTTATTA ATGAATTTTC CTGCTGTAAT AATGGGTAGA AGGTAATTAC TATTATTATT GATATTTAAG TTAAACCCAG TAAATGAAGT CCATGGAATA ATAGAAAGAG AAAAAGCATT TTCAGGTATA GGTGTTTTGG GAAACAATTT CCCCGAACCA TTATATTTCT CTACATCAGA AAGGTATAAA TCATAAAACT CTTTGAAGTC ATTCTTTACA GGAGTCCAAA TACCAGAGAA TGTTTTAGAT ACACCATCAA AAATTGTATA AAGTGGCTCT AACTTATCCC AATAACCTAA CTCTCCGTCG CTATTGTAAC CAGTTCTAAA AGCTGTATTT GAGTTTATCA CCCTTGTCAC TAAGAAAATA AATGCAGGGT AAAATTTATA TCCTTCTTGT TTTATGTTTC GGTATAAAAC ACTAATATCA ATTTCTGTGG TTATACTAAA AGTCGTTTGT TGGTTCAAAT AATGATTAAA TATCTCTTTT CTCTTCCAAT TGTCTAAATC AATTTTATTA AAGTTCATTT GATATGCCTC CTGGCCCATC AGTCTGACGA CCAAGAGAGC CATAAACACC AATAGCCTTA ACATCATCCC CATATTTATC CAATATTCGT TCCTTAATTT CATGAACAAT CTTCATTCTT TCTTCTCTAG TCATTATTAT TGGTCCATTC ACTATTCTCA TTCCCTTTTC AGATAATTTT AGATTTGCTT TTCTAAATAA GAATATTTGG AGAGCACCGT TCTTATTCAG CTATTAATAA CTCGTCTTCC TAAGCATCCT TCAATCCTTT TAATAACAAT TATAGCATCT AATCTTCAAC AAACTGGCCC GTTTGTTGAA CTACTCTTTA ATAAAATAAT TTTTCCGTTC CCAATTCCAC ATTGCAATAA TAGAAAATCC ATCTTCATCG GCTTTTTCGT CATCATCTGT ATGAATCAAA TCGCCTTCTT CTGTGTCATC AAGGTTTAAT TTTTTATGTA TTTCTTTTAA CAAACCACCA TAGGAGATTA ACCTTTTACG GTGTAAACCT TCCTCCAAAT CAGACAAACG TTTCAAATTC TTTTCTTCAT CATCGGTCAT AAAATCCGTA TCCTTTACAG GATATTTTGC AGTTTCGTCA ATTGCCGATT GTATATCCGA TTTATATTTA TTTTTCGGTC GAATCATTTG AACTTTTACA TTTGGATCAT AGTCTAATTT CATTGCCTTT TTCCAAAATT GAATCCATTG TTTTTGATTC ACGTAGTTTT CTGTATTCTT AAAATAAGTT GGTTCCACAC ATACCAATAC ATGCATGTGC TGATTATAAG AATTATCTTT ATTATTTATT GTCACTTCCG TTGCACGCAT AAAACCAACA AGATTTTTAT TAATTTTTTT ATATTGCATC ATTCGGCGAA ATCCTTGAGC CATATCTGAC AAACTCTTAT TTAATTCTTC GCCATCATAA ACATTTTTAA CTGTTAATGT GAGAAACAAC CAACGAACTG TTGGCTTTTG TTTAATAACT TCAGCAACAA CCTTTTGTGA CTGAATGCCA TGTTTCATTG CTCTCCTCCA GTTGCACATT GGACAAAGCC TGGATTTACA AAACCACACT CGATACAACT TTCTTTCGCC TGTTTCACGA TTTTGTTTAT ACTCTAATAT TTCAGCACAA TCTTTTACTC TTTCAGCCTT TTTAAATTCA AGAATATGCA GAAGTTCAAA GTAATCAACA TTAGCGATTT TCTTTTCTCT CCATGGTCTC ACTTTTCCAC TTTTTGTCTT GTCCACTAAA ACCCTTGATT TTTCATCTGA ATAAATGCTA CTATTAGGAC ACATAATATT AAAAGAAACC CCCATCTATT TAGTTATTTG TTTAGTCACT TATAACTTTA ACAGATGGGG TTTTTCTGTG CAACCAATTT TAAGGGTTTT CAATACTTTA AAACACATAC ATACCAACAC TTCAACGCAC CTTTCAGCAA CTAAAATAAA AATGACGTTA TTTCTATATG TATCAAGATA AGAAAGAACA AGTTCAAAAC CATCAAAAAA AGACACCTTT TCAGGTGCTT TTTTTATTTT ATAAACTCAT TCCCTGATCT CGACTTCGTT CTTTTTTTAC CTCTCGGTTA TGAGTTAGTT CAAATTCGTT CTTTTTAGGT TCTAAATCGT GTTTTTCTTG GAATTGTGCT GTTTTATCCT TTACCTTGTC TACAAACCCC TTAAAAACGT TTTTAAAGGC TTTTAAGCCG TCTGTACGTT CCTTAAGGAA TTCGAGCTCA GCATTATTGA GTGGATGATT ATATTCCTTT TGATAGGTGG TATGTTTTCG CTTGAACTTT TAAATACAGC CATTGAACAT ACGGTTGATT TAATAACTGA CAAACATCAC CCTCTTGCTA AAGCGGCCAA GGACGCTGCC GCCGGGGCTG TTTGCGTTTT TGCCGTGATT TCGTGTATTA TTGGTTTACT TATTTTTTTG CCAAAGCTGT AATGGCTGAA AATTCTTACA TTTATTTTAC ATTTTTAGAA ATGGGCGTGA AAAAAAGCGC GCGATTATGT AAAATATAAA GTGATAGCGG TACCATTATA GGTAAGAGAG GAATGTACAC ATGAAGAAAC CGTTGGGGAA AATTGTCGCA AGCACCGCAC TACTCATTTC TGTTGCTTTT AGTTCATCGA TCGCATCGGC TGCTGAAGAA GCAAAAGAAA AATATTTAAT TGGCTTTAAT GAGCAGGAAG CTGTCAGTGA GTTTGTAGAA CAAGTAGAGG CAAATGACGA GGTCGCCATT CTCTCTGAGG AAGAGGAAGT CGAAATTGAA TTGCTTCATG AATTTGAAAC GATTCCTGTT TTATCCGTTG AGTTAAGCCC AGAAGATGTG GACGCGCTTG AACTCGATCC AGCGATTTCT TATATTGAAG AGGATGCAGA AGTAACGACA ATGGGATCCT CTAGAGTCGA CCTGCAGCTC GAGCACCACC ACCACCACCA CAAGCTTGTA TTCTATAGTG TCACCTAAAT CGTATGTGTA TGATACATAA GGTTATGTAT TAATTGTAGC CGCGTTCTAA CGACAATATG TACAAGCCTA ATTGTGTAGC ATCTGGCTTA CTGAAGCAGA CCCTATCATC TCTCTCGTAA ACTGCCGTCA GAGTCGGTTT GGTTGGACGA ACCTTCTGAG TTTCTGGTAA CGCCGTCCCG CACCCGGAAA TGGTCAGCGA ACCAATCAGC AGGGTCATCG CTAGCCAGAT CCTCTACGCC GGACGCATCG TGGCCGGCAT CACCGGCGCC ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA TCGGGCTCGC CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG GGACTGTTGG GCGCCATCTC CTTGCACCAT TCCTTGCGGC GGCGGTGCTC AACGGCCTCA ACCTACTACT GGGCTGCTTC CTAATGCAGG AGTCGCATAA GGGAGAGCGT CGATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
