pCMV-SPORT6-UPF1
$99.00
In stock
SKU
PL75789
NM_002911.4;HUPF1;NORF1;pNORF1;RENT1;smg-2
100μg liquid plasmid(Instantly):$120
| name | pCMV-SPORT6-UPF1 | another name | apCMV-SPORT6-UPF1 |
| Insert Size (bp) | 3357bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-SPORT6 | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CCAGTGCCTA GCTTATAATA CGACTCACTA TAGGGACCAC TTTGTACAAG AAAGCTGGGT ACGCGTAAGC TTGGGCCCCT CGAGGGATAC TCTAGAGCGG CCGCCCTTTT TTTTTTTTTT TTGAAGTCAA AACTGTTTAT TAATATTTGG TGACAAAAGA ACTTAAATTG ACCGAAAATC AAAAGTTACA TTGCCTTGGT ACAGTGCGCC TGGGTTTGTC AAGGCTGCTG CTACAAAGCT GGAGAACACA GCACAGGGCG AAGCAGAGGC AAAGCGGCCC AGCCCAACAG CCGGGATGGG GCAGGGAGCG TCCCTCAGGC CGCCGCGGGG TCACCGCGGA AACCTACCCG GTGCATGCCT CTACGCGGCC TACCGGAAGA AGGGGAAACC GAAAGCCCTA GAGCAATGGG AATAAATAAG AGTCCCTGCC AGAAGGAAGG TGGCCTGTGC GTCCCCGTCT GTCCCATGTA GAAGCCGGAA TCTGGAGGTG TTCGGATCAG GGCCAGTGAC AGCCCCAGGC CCGGGGCAGG GGAAGCTGGA GGCCTGGCCG CCAGGCAGAA TGACACCCCA AGCCAGGCGT TCGAGGGGCT GGGGCAGCTC TCCTAAAATG ACCCTCTGCC AATGACACCA CACCCTCAGT CCCTTCACAC AGAACGCGGT AATTTCTGTA ACTTGTTTCC TTACCCCCCA GAAGCAGCAA TCACCCCATC AGCAAAGTTG CCGGCAGGCA GAGCTCCCAA TTTGGGCTTT CGGTGTGCAG CGCTCCGTGT CTAACCAGCA CAGGGACTGT CGGTACCTCC TGGGGACGGG GACAGGGACA GGCCCTCTTG TCCTTCCACC AAATCTTCCA AAGGCCCCCG GGAAAAAGCC GTCAATCCGG GAACAGCCAC CTTCTCGCCT CCTGGCCACA GGGCCAGTCC TGGCCAAGGC CCCTCGCAAT GGGGCCAAAA GCACCTTCCT CACCGGTGCG GGGCCCTGCG CCGAGTCTAG GCGGGAAGCA GCTTGGCTTC TGAGTCCAGC GGAGAACGAG CAGCTTCCAG GCTCGGCGGG CGGCTACCCC AGCAGCCTGA TGCCCCGCAG AAAGAAGCCA CTGGAAGCTA AAACCAACAA CAAAACTCCT CCGGCCGCGG CACAGGTGTT GCTCCTCTTC TCCAGTAGCG CTGGCTTGTT TAAACTGTTT CTGGAAGCTT CTCAAATCTC CCAGGTCCAC CGCTAAGATT TTAGAGACAA ACTTAGGAAA GTATTCTTCA GTACTCCTTT AAAAGAATCT TGAAAAAAAC AAAGAGAAAA ACCCAAAACA CCCTTATTTT CTTTAAATGA AGGCCCTCGG AGAACACAGG CAGCGCGACC TCGGCGCGTC CGGTAGGCGT GGACAGACGC GCCCTCCCGG GGCTCTGCCC CGCACGCTGC TGTCTTTGCA AGGAGCCTGC TCGGGCACAG AAATGCGAAG TTTTTTTTCC TGCTAAGACG TAGTCCCCTT TCAATCAAAG AGAAAAAGAA ATAAAAGAAC AGAGGAAAGG GCTACTCTGA GATGAGGGTC TTCCTCCCTC CGCCCTGGCC GCAGCCCCGA TGGGACGGAG CGGGGCCTCC TGGTGGCCGC GGCTCCCGCG GGCCGGCCCC TCCTCCGCTC CCTCTGCTCC TCGCCGCCGC GGGCCAGCAG GGGGCGCTCG GCCTCGGCTC CTCTCCCTCG TGCGCTCTCC CTTCCTGTTG CTCACACAGT GGTGCTTCAG TTTTAGCAGT GGAAAACAGG TATCCGTTTA TTTTTATTTT TTATTACCCA GAATAAGATG CTGATGGACT AAGCCACGTT GCTTAGCTCT TCCGCCGCCA CCTTTTAATA CTGGGACAGC CCCGTCACCC CGCCATGCTG GTAAGCCCGC TCTCCCTGGT ACGTGGAGTC CTGTGAGAGC GCCACGTCGA TTTGTGATTT AAACTCGTCA CCAAGGTAAC TGTCCTGGGA CAGCTCCGGC TGGGAGAGGC CGGGCTGGCT CATCTGGGAA GGCTGGCTCA TGGAGATGTA GCCCTGCGTC AGGGCGCCCT GAGAGAAGGG CTGTGACGCC ACATCCTGGC TGGCTTGGCT GTTGGGGAGG TTAGTCTGGC TGGGTCCAGG AAGCCCAAAG CGGTTCTTCT GGCGTCCCCC ACGACCAGTC TTGCCTTTCG GGGTGCCTCG CCCTGCAGCA GGCCCGTTGG CTTGTCCAAA ATAGCCAGGC GGTGGCATGG GTGGCATGAC CAGGTTGAAG GGGATGGGAA TGTTCATTGG CAGCCACGTG GCTAGGGCCG GCACTGATCA TGCCAATCTG GTCATGGGTC TGGAAGTACA TGCTGGAAGG CCGGCCCTGG CTGCTCCGAT CATAGACGGA GCCTGGGATG ATGGCCTCCC GGGCATCATA CATGGCTGTG GTCATGAAGC GGGCTCCCGG GTTGATAGTG TTGACCAGCT TCCGTGGCTT GCTGAACTGC ATGAGGCTCT CACGCAGGTT GTTGAGCGGC CCCTCCACCA GCACCTTCTG CTCCTTATAG TAGTTCAGCA GGTGGTTCCA GAGCGGCTGC TTTGATAGTG CCTTCGGGTT GCCCACAATG ATGACGCCAT ACCTTGCTCT GGTCAGGGCC ACGTTCAGAC GCCTGGGGTC ATTTAAAAAG CCAATGCCTT GGTGCTCGTT GGCCCGCACA CAGGACAGGA TGATGAAGTC CTTCTCGCGT CCCTGAAAGG CGTCCACACT GGCGATCTCC ACCTCCTGGT AGAGCTTGGT GTGCAGGGAG CCGCTGAACT GCATGTACTG CACCAGGTAG GAGCGCTGGC CCTCGTAGGG CGTGATGATG CCAATCTGGT CCGGCTTGGC GCCTGCCTTC AGCAACTTCG TGGTGATCTT CTCCACGTTC GCAGCCTCGG TCCTGTTCAG GTAGGAGGTG CCCGAGCTGG CAATCTCCTC TTGGCCCTGG GTCACGTAGA AGAACATCGG TTTATCGGGT TGGGGCCACT GGAAGTCAAA TCCCTTCTTC ACACGATCCG CTGCAGTGAC ACCATTCTGG AGGGAGCCCT CGTAGAAGAT GTTGGATGGG AAGGCGCTGA GTGCAGGGTG CATCCGGTAC TGGACCTGCA GGCGGATGGG CCGGATGCCC AGCACCACCA GGCGCTCGAA GAGCGACTGT GACAGCCCGG CCTTGGCCGC CTTCTTGCAC ATCACCACTG GGCCCAGCTG GCAGTGGTCG CCTACAAGGA TCAGCTGCTT GGCCCCGAGG ACCACGGGAA CCATGCACTC CGGCTCGGTG GCCTGGGTGC TTTCGTCGAT TAAAATGGAG CGGAACTGCA TCTTGGCCAG CCTCGGGTCA CCGGCGCCCA CACATGTGCA GCAGATGACA TCTGCGTTCA TCAGCAGCTC TCTCTCTGCG GTGCGCTTCA AGGCCCGGTA CCGCTTCTCG TCGGCAGACG ACAGCTCCCC AGTCTCGTCT TTCAGCTGCT GCAGCTTCTG CAGCTCAGGC ATGCTGTCCA TGTTCCTGAT CTGGTTGTGC AGGGCCAGAA AAGACACCGG GGAGTCGATG GCCTCACGGC TCTTGGCGCA GAGGCGCACG ACCTTTAGCC CCGTCTGGTG GATCTTCTCC GTTAGCTGGT CCACGGCGAT GTTGCTCGGA GCACACACCA GCACCGGCCC GTTGCCTTGC CGGGCCAGGT GGTAGACGAT GGTGGCCGAC GTCACCGTCT TCCCCGTGCC TGGCGGGCCC TGGATCAGGC TCAGTGGTCT TTGCAGCACA GTCTTCACGG CATAAACCTG GGAGTGGTTG AGGTCGGGGA GGCCCTGCGC CGTGAAGCGC TTGGGCAGCT GGCACTTGAT GATTACGTCC TCCACCTCGT GGCCCAACAG CTTGTGGTAG ATGTAGCCAG ACACCGAGGT CTCATCCACG GCAAACGTTT TCAATGCGCT CTGCATCCTG TCAAAGGAGG TCGACTTCCA CACAAAATCC ACCTGGAAGT TGTGAGTCAC CTCCACAGGT GCACCCACGC TGCTCCGCAG CTCAATGGCG ATCTCATCGC CATAATTATC AGGGACCTTG ATGACGTGGC CGATCCCTTT CCACAGGGGC GCAAGGTCCC CTTTGTACCG CAGGCATATC TCATCCCCCT GCATGAGCCG CATGTCAGAG TCAGTCTTGG GCAAAGTGAA GTAGGCGATT CTCTTCTTGT TAAGGCCCAG GTCCCACCTG ACAGTGATGT TATCTTGAGT CTGGGACTCC TTCAGCTTCT TGTCGTAGTC GGCCTCCAGC TTGACCAGGG GCCCGAATAT GTTCTGGTAC TGGTAGGCGT CCTCGTACCG CAGGAGGACA TGCTGCGGCT CCTCGTCCAC CCCCGGCTTC TCCAGGTCCT CCAGCGTGGC AGAAGGGTTT TCCTTCCACA GCTCCTCCAG CTTGTTGATC TGCTGTGCCG TGATCTGGCG TGCCCGCAGC TGCTCCTGCT CGGAGGGGAT CTTGACCAGC CAGGACAGGA AGCAGCGGTC CTGGATCAGC GGCTGCCACT GCGAGCTGTC CCAGTTGATG TCCTTGAGGC TGCTCTGGCT GGCACAGGGC TGCCTGCACA GCAGCACCAC CACTGAGTCA GCTTTGGCCG GGATGAAGCC GAGGAGGAAG ACGTTGCGAC AGCCGCAGTT GTAGCACTCC AGGACTGTCT CCCCCAGGGG CCCGTCCTTG TGCAGGGTCA CCTCTTTGCA TTTTGCCCTC ACAAGGTGAT TTACAATGTG GCTGCCAGAA GTATTTCCAC GTCCGTTGCA GAACCACTTC TTGCTGGTAT TACAGTAAAC CACGCAGGCA GGATCGTGTA TTCCACAGTA ACTGCAGGCG TGTATGGGGA GGTCCTTCGT GTAATAGGTG TCTTCTTCAT CTTCCTCGAA GTTCAACTCA GCCAACAACT GGCTGGTCTT GGCTACACTG TCGTCCACAG CCCCGTTCTG CAGGATGCCT TCGGGCCCAA CCTGCGCGTC GAGCTGTCCC GCCGCAGCGC CCGCGCCCGC GCCGCCCGGG CCTCCCGCGC CGCCACCGCC CGGGCCGCCG GGGCCGCCGG GGGGCGTCTG CGTCTGGCTA GGAAGAGTAA AGTCGGTGAA CTCGAACTCG GAGCCCTGTG TGTCGGCGCC AAGCAGCTCG GCCTCCTCCG TGTCCAGGAA AGTGAGAGTC TGCGAGCTGG GCCCGTACGC CTCCACGCTC ATGGTGCCTC CGGGTAGGGC CCTCGGGCCG GTTCCGCGCT GCACTCGAGC CCGCCGCCGC GCTGAGGCCT AGGCCGCAGG CCTCGGGTCG CTGCCGCTGT CGCCCCCGCG CGCCCGGAAA GGACCAAACC GCCCGCCCCG GCGCCAGCCG GACCGAGAGG TAACAGTGCC GAGCCGCCGC CGCTGCCGCC GAGCCCCTCC GCGCCTCAGC TCCCCTCGAA GCCGCCAGCC GCTCGCAGCC TAGAGCAGGA ACTGCCGCCA CCGCCGCCGT CGCCCGGACG CGTGGGTCGA CGATATCCCG GGAATTCCGG ACCGGTACCA GCCTGCTTTT TTGTACAAAC TTGTTCTATA GTGTCACCTA AATAGGCCTA ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC CGCGGCCTAG GCTAGAGTCC GGAGGCTGGA TCGGTCCCGG TGTCTTCTAT GGAGGTCAAA ACAGCGTGGA TGGCGTCTCC AGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGACATGCA TCTAATGAGT GAAAGGGCCT CGTACTACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAC GCGCGAATTG CAAGCTCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGCCAT AACTTCGTAT AGCATACATT ATACGAAGTT ATGGCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGCCAG GGGTGGGCAC ACATATTTGA TACCAGCGAT CCCTACACAG CACATAATTC AATGCGACTT CCCTCTATCG CACATCTTAG ACCTTTATTC TCCCTCCAGC ACACATCGAA GCTGCCGAGC AAGCCGTTCT CACCAGTCCA AGACCTGGCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAAATTGTAA ACGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCC AATACGCAAA CCGCCTCTCC CCGCGCGTTG GCCGATTCAT TAATGCAGGA TCGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGT GAAAAAAATG CTTTATTTGT GAAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA GCTGCAATAA ACAAGTTAAC AACAACAATT GCATTCATTT TATGTTTCAG GTTCAGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCATGAACA GACTGTGAGG ACTGAGGGGC CTGAAATGAG CCTTGGGACT GTGAATCTAA AATACACAAA CAATTAGAAT CACTAGCTCC TGTGTATAAT ATTTTCATAA ATCATACTCA GTAAGCAAAA CTCTCAAGCA GCAAGCATAT GCAGCTAGTT TAACACATTA TACACTTAAA AATTTTATAT TTACCTTAGA GCTTTAAATC TCTGTAGGTA GTTTGTCCAA TTATGTCACA CCACAGAAGT AAGGTTCCTT CACAAAGATC CCAAGCTAGC AGTTTTCCCA GTCACGACGT TGTAAAACGA CGG
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