pGL4.36[luc2P MMTV Hygro]
$84.00
In stock
SKU
PL75768
100μg liquid plasmid(Instantly):$105
| name | pGL4.36[luc2P MMTV Hygro] | another name | apGL4.36[luc2P MMTV Hygro] |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | Fluc |
| Promoter | MMTV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | RVP3：CTAGCAAAATAGGCTGTCCC | |
| 3′ sequencing primer | GLP4：GGGTAGAATGGCGCTGGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGCCTAACTG GCCGCAGAAA TGGTTGAACT CCCGAGAGTG TCCTACACCT AGGGGAGAAG CAGCCAAGGG GTTGTTTCCC ACCAAGGACG ACCCGTCTGC GCACAAACGG ATGAGCCCAT CAGACAAAGA CATATTCATT CTCTGCTGCA AACTTGGCAT AGCTCTGCTT TGCCTGGGGC TATTGGGGGA AGTTGCGGTT CGTGCTCGCA GGGCTCTCAC CCTTGACTCT TTCAATAATA ACTCTTCTGT GCAAGATTAC AATCTAAACA ATTCGGAGAA CTCGACCTTC CTCCTGAGGC AAGGACCACA GCCAACTTCC TCTTACAAGC CGCATCGATT TTGTCCTTCA GAAATAGAAA TAAGAATGCT TGCTAAAAAT TATATTTTTA CCAATAAGAC CAATCCAATA GGTAGATTAT TAGTTACTAT GTTAAGAAAT GAATCATTAT CTTTTAGTAC TATTTTTACT CAAATTCAGA AGTTAGAAAT GGGAATAGAA AATAGAAAGA GACGCTCAAC CTCAATTGAA GAACAGGTGC AAGGACTATT GACCACAGGC CTAGAAGTAA AAAAGGGAAA AAAGAGTGTT TTTGTCAAAA TAGGAGACAG GTGGTGGCAA CCAGGGACTT ATAGGGGACC TTACATCTAC AGACCAACAG ATGCCCCCTT ACCATATACA GGAAGATATG ACTTAAATTG GGATAGGTGG GTTACAGTCA ATGGCTATAA AGTGTTATAT AGATCCCTCC CCTTTCGTGA AAGACTCGCC AGAGCTAGAC CTCCTTGGTG TATGTTGTCT CAAGAAAAGA AAGACGACAT GAAACAACAG GTACATGATT ATATTTATCT AGGAACAGGA ATGCACTTTT GGGGAAAGAT TTTCCATACC AAGGAGGGGA CAGTGGCTGG ACTAATAGAA CATTATTCTG CAAAAACTTA TGGCATGAGT TATTATGATT AGCCTTGATT TGCCCAACCT TGCGGTTCCC AAGGCTTAAG TAAGTTTTTG GTTACAAACT GTTCTTAAAA CAAGGATGTG AGACAAGTGG TTTCCTGACT TGGTTTGGTA TCAAAGGTTC TGATCTGAGC TCTGAGTGTT CTATTTTCCT ATGTTCTTTT GGAATTTATC CAAATCTTAT GTAAATGCTT ATGTAAACCA AGATATAAAA GAGTGCTGAT TTTTTGAGTA AACTTGCAAC AGTCCTAACA TTCACCTCTT GTGTGTTTGT GTCTGTTCGC CATCCCGTCT CCGCTCGTCA CTTATCCTTC ACTTTCCAGA GGGTCCCCCC GCAGACCCCG GCGACCCTCA GGTCGGCCGA CTGCGGCAGG CCTCGGCGGC CAAGCTTGGC AATCCGGTAC TGTTGGTAAA GCCACCATGG AAGATGCCAA AAACATTAAG AAGGGCCCAG CGCCATTCTA CCCACTCGAA GACGGGACCG CCGGCGAGCA GCTGCACAAA GCCATGAAGC GCTACGCCCT GGTGCCCGGC ACCATCGCCT TTACCGACGC ACATATCGAG GTGGACATTA CCTACGCCGA GTACTTCGAG ATGAGCGTTC GGCTGGCAGA AGCTATGAAG CGCTATGGGC TGAATACAAA CCATCGGATC GTGGTGTGCA GCGAGAATAG CTTGCAGTTC TTCATGCCCG TGTTGGGTGC CCTGTTCATC GGTGTGGCTG TGGCCCCAGC TAACGACATC TACAACGAGC GCGAGCTGCT GAACAGCATG GGCATCAGCC AGCCCACCGT CGTATTCGTG AGCAAGAAAG GGCTGCAAAA GATCCTCAAC GTGCAAAAGA AGCTACCGAT CATACAAAAG ATCATCATCA TGGATAGCAA GACCGACTAC CAGGGCTTCC AAAGCATGTA CACCTTCGTG ACTTCCCATT TGCCACCCGG CTTCAACGAG TACGACTTCG TGCCCGAGAG CTTCGACCGG GACAAAACCA TCGCCCTGAT CATGAACAGT AGTGGCAGTA CCGGATTGCC CAAGGGCGTA GCCCTACCGC ACCGCACCGC TTGTGTCCGA TTCAGTCATG CCCGCGACCC CATCTTCGGC AACCAGATCA TCCCCGACAC CGCTATCCTC AGCGTGGTGC CATTTCACCA CGGCTTCGGC ATGTTCACCA CGCTGGGCTA CTTGATCTGC GGCTTTCGGG TCGTGCTCAT GTACCGCTTC GAGGAGGAGC TATTCTTGCG CAGCTTGCAA GACTATAAGA TTCAATCTGC CCTGCTGGTG CCCACACTAT TTAGCTTCTT CGCTAAGAGC ACTCTCATCG ACAAGTACGA CCTAAGCAAC TTGCACGAGA TCGCCAGCGG CGGGGCGCCG CTCAGCAAGG AGGTAGGTGA GGCCGTGGCC AAACGCTTCC ACCTACCAGG CATCCGCCAG GGCTACGGCC TGACAGAAAC AACCAGCGCC ATTCTGATCA CCCCCGAAGG GGACGACAAG CCTGGCGCAG TAGGCAAGGT GGTGCCCTTC TTCGAGGCTA AGGTGGTGGA CTTGGACACC GGTAAGACAC TGGGTGTGAA CCAGCGCGGC GAGCTGTGCG TCCGTGGCCC CATGATCATG AGCGGCTACG TTAACAACCC CGAGGCTACA AACGCTCTCA TCGACAAGGA CGGCTGGCTG CACAGCGGCG ACATCGCCTA CTGGGACGAG GACGAGCACT TCTTCATCGT GGACCGGCTG AAGAGCCTGA TCAAATACAA GGGCTACCAG GTAGCCCCAG CCGAACTGGA GAGCATCCTG CTGCAACACC CCAACATCTT CGACGCCGGG GTCGCCGGCC TGCCCGACGA CGATGCCGGC GAGCTGCCCG CCGCAGTCGT CGTGCTGGAA CACGGTAAAA CCATGACCGA GAAGGAGATC GTGGACTATG TGGCCAGCCA GGTTACAACC GCCAAGAAGC TGCGCGGTGG TGTTGTGTTC GTGGACGAGG TGCCTAAAGG ACTGACCGGC AAGTTGGACG CCCGCAAGAT CCGCGAGATT CTCATTAAGG CCAAGAAGGG CGGCAAGATC GCCGTGAATT CTCACGGCTT CCCTCCCGAG GTGGAGGAGC AGGCCGCCGG CACCCTGCCC ATGAGCTGCG CCCAGGAGAG CGGCATGGAT AGACACCCTG CTGCTTGCGC CAGCGCCAGG ATCAACGTCT AAGGCCGCGA CTCTAGAGTC GGGGCGGCCG GCCGCTTCGA GCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA CAACTAGAAT GCAGTGAAAA AAATGCTTTA TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGGTG TGGGAGGTTT TTTAAAGCAA GTAAAACCTC TACAAATGTG GTAAAATCGA TAAGGATCCG TTTGCGTATT GGGCGCTCTT CCGCTGATCT GCGCAGCACC ATGGCCTGAA ATAACCTCTG AAAGAGGAAC TTGGTTAGCT ACCTTCTGAG GCGGAAAGAA CCAGCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGAAGA AGCCCGAACT CACCGCTACC AGCGTTGAAA AATTTCTCAT CGAGAAGTTC GACAGTGTGA GCGACCTGAT GCAGTTGTCG GAGGGCGAAG AGAGCCGAGC CTTCAGCTTC GATGTCGGCG GACGCGGCTA TGTACTGCGG GTGAATAGCT GCGCTGATGG CTTCTACAAA GACCGCTACG TGTACCGCCA CTTCGCCAGC GCTGCACTAC CCATCCCCGA AGTGTTGGAC ATCGGCGAGT TCAGCGAGAG CCTGACATAC TGCATCAGTA GACGCGCCCA AGGCGTTACT CTCCAAGACC TCCCCGAAAC AGAGCTGCCT GCTGTGTTAC AGCCTGTCGC CGAAGCTATG GATGCTATTG CCGCCGCCGA CCTCAGTCAA ACCAGCGGCT TCGGCCCATT CGGGCCCCAA GGCATCGGCC AGTACACAAC CTGGCGGGAT TTCATTTGCG CCATTGCTGA TCCCCATGTC TACCACTGGC AGACCGTGAT GGACGACACC GTGTCCGCCA GCGTAGCTCA AGCCCTGGAC GAACTGATGC TGTGGGCCGA AGACTGTCCC GAGGTGCGCC ACCTCGTCCA TGCCGACTTC GGCAGCAACA ACGTCCTGAC CGACAACGGC CGCATCACCG CCGTAATCGA CTGGTCCGAA GCTATGTTCG GGGACAGTCA GTACGAGGTG GCCAACATCT TCTTCTGGCG GCCCTGGCTG GCTTGCATGG AGCAGCAGAC TCGCTACTTC GAGCGCCGGC ATCCCGAGCT GGCCGGCAGC CCTCGTCTGC GAGCCTACAT GCTGCGCATC GGCCTGGATC AGCTCTACCA GAGCCTCGTG GACGGCAACT TCGACGATGC TGCCTGGGCT CAAGGCCGCT GCGATGCCAT CGTCCGCAGC GGGGCCGGCA CCGTCGGTCG CACACAAATC GCTCGCCGGA GCGCAGCCGT ATGGACCGAC GGCTGCGTCG AGGTGCTGGC CGACAGCGGC AACCGCCGGC CCAGTACACG ACCGCGCGCT AAGGAGGTAG GTCGAGTTTA AACTCTAGAA CCGGTCATGG CCGCAATAAA ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGCG GCCGCAAATG CTAAACCACT GCAGTGGTTA CCAGTGCTTG ATCAGTGAGG CACCGATCTC AGCGATCTGC CTATTTCGTT CGTCCATAGT GGCCTGACTC CCCGTCGTGT AGATCACTAC GATTCGTGAG GGCTTACCAT CAGGCCCCAG CGCAGCAATG ATGCCGCGAG AGCCGCGTTC ACCGGCCCCC GATTTGTCAG CAATGAACCA GCCAGCAGGG AGGGCCGAGC GAAGAAGTGG TCCTGCTACT TTGTCCGCCT CCATCCAGTC TATGAGCTGC TGTCGTGATG CTAGAGTAAG AAGTTCGCCA GTGAGTAGTT TCCGAAGAGT TGTGGCCATT GCTACTGGCA TCGTGGTATC ACGCTCGTCG TTCGGTATGG CTTCGTTCAA CTCTGGTTCC CAGCGGTCAA GCCGGGTCAC ATGATCACCC ATATTATGAA GAAATGCAGT CAGCTCCTTA GGGCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCGGTGT TGTCGCTCAT GGTAATGGCA GCACTACACA ATTCTCTTAC CGTCATGCCA TCCGTAAGAT GCTTTTCCGT GACCGGCGAG TACTCAACCA AGTCGTTTTG TGAGTAGTGT ATACGGCGAC CAAGCTGCTC TTGCCCGGCG TCTATACGGG ACAACACCGC GCCACATAGC AGTACTTTGA AAGTGCTCAT CATCGGGAAT CGTTCTTCGG GGCGGAAAGA CTCAAGGATC TTGCCGCTAT TGAGATCCAG TTCGATATAG CCCACTCTTG CACCCAGTTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCGGGGTGT GCAAAAACAG GCAAGCAAAA TGCCGCAAAG AAGGGAATGA GTGCGACACG AAAATGTTGG ATGCTCATAC TCGTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA CTAGTACGTC TCTCAAGGAT AAGTAAGTAA TATTAAGGTA CGGGAGGTAT TGGACAGGCC GCAATAAAAT ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG TACTAACATA CGCTCTCCAT CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT GCCAGAACAT TTCTCT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
