pLV3-U6-SLC27A2(human)-shRNA1-CopGFP-Puro
$55.00
In stock
SKU
PL75739
100μg liquid plasmid(Instantly):$76
| name | pLV3-U6-SLC27A2(human)-shRNA1-CopGFP-Puro | another name | apLV3-U6-SLC27A2(human)-shRNA1-CopGFP-Puro |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Interfere with silence | Fragment type | shRNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | U6-F：GAGGGCCTATTTCCCATGA | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGCGTTAGAT ACTCAAGCTA TGCATCAAGC TTGGTACCGA GCTCGGATCC ACTAGTAACG GCCGCCAGTG TGCTGGAATT CGCCCTTCCG GGAGATCACA GCTTGGGGGT TTGAATGAAC CCTGTACCAA GGAGTTGAGA CAACTCCTTC TTTGGGTCAC ATACTGGAAC CCTTAGCATT CAAAAGCTGC TCAGCAAAGA TAATTTCAGC ATTTCAGTGG CATCTTTCTC TGCTGTCGTA TTTCATTCAG AAGCCATTTT AGCATGTCCC CCATATATGG CTTTTCATGA CAAATCACTG TGATGCCACA CAAAACTGCT TTAAGTGTTT TTTCAGTATA AAAGGGTTTT GCATTTAGAG CTCAATATCA GAATTAAGCA TGTTCTGTAA AAGCTTAATT TAACCCATTT AATATACTTG TTTTTAAACG AGTCTATCCT GATTTTCCAT CCTAGATAAA ATTTTAGCCC AATTGCAGTA CTTTCCAATC ACCTTGGGCC GGGAGGGAAA ATTTTTAGGT ATAGAACCCA ACACAAAGAT GCTAAATGTA TTCTTCGTGA CTTAATTTTC AATCCATATT GTATATGGAT TCTACCGAGG GAAAATATTC TCATTTGAAT AGGGAGAAAT GATCTTTTTC AGGTAATTCT TTTCTTGGCA TTTACGAGTG CTGACTGTCA TGCTGAACAT GGATATCCAA CAGGAATCAA GGGGAAAAGC TTAATTTATA TTGTTTTGAA AATAGATGTT TCTATGATCA TAAAAATCTT TTTATATATT CCTTAAATAT TTTGGATGGT GATTTAATAC ATAAACATAT TTGTACAGGT TTTTTTAAAA ACACTTTGTA ATTTTGCTTA CTCAACAAAA ATGAGTAATT ATGTAAAATA TTTTTTGTCA TTTTATAAAA TATGTCTTTT TGGTATTGTT TTTGGATAAT GCATGACCTG TCATTTTCCA AGTAATGAAT CCCTGATGGA TTACATAGTT ACATGACTAT GTCATGAATT ATACATTTCT TGTCACATAT TTGAGGCAAA CCTGAGGATA GATTATTGAA TAATTGTTAT TATTTTCTTT CGTAAAATAT TTCATTTCTC CATTTTCTTT TTCAGTGGTC TTTTGAAGAA TCCAATCTTC CACAGTGCTA AAATGAGCAG CATTAACAGC AACAATCCGG CAAAAGCACT CAGCAGGATG ACCCATAATG GCACTCTGCC CGGTAGCCCA TCTTTGGATA TTTGAATAGC AAGCTCTCTT TTTTGATTGC TGCTACTTAA AACCAGAGAT GCATTTTCAC TCCGAAGTTC TCCCCTTATA GTAAGATTTA AGCTGGAAAA ATATGATTTT ATAAAAGTTG GTTTCCACAA GATAAGCGAA ACATTGACTT GGCTGATGTC AGAAGAAGTG AGATTACATG TGATGGTAGC AAATTTACAT GTATTGCAGT CCAGAATTGT GCCTCGTTTG AGATGGTCAG TTGATGTAGT CATTTTCTTT CCAGAGTTGA TACTGAAAGG ATCCTCAAAG ATATGGGGTC TGCAGTTTGC ATTCTCAGAA GATGACAATC CAGTTGGGTA CAGCACAGGG TAACCATTTG ATGTCATATT GGGGAATGAA ATTGACAGCT TAAGCTCTGG CATTGGAAAA GATCCACTTT TTCTAATCAA GTAGAAGATA TTAATTTCAT TTCCAATGTC CTCAGTAGAA TTAATAACTT CAGGGACTGT CTCATTGGCA GCAATTGAAA TGTGGTATTC ACTTGCAGAG CTGTAAAACT GTAGTCCAAC TTCATATTTT ACCGGGATAG AAATGTTTAC TACATTATCA GAAAGGGTTT CAGGAGGTTC TTCGCTGTCA CTTGTTGCAC TTAAATAAAT GGTCACATTT TCCATGAGAT AGGATGTGTT AAACTGAAAC AATATTTTGA AAGTTACCAT CTCTCCTCTT CTCAGGAAGG GATATCCAAC TTTACATGTG ATATTATGAT TAGATTCACA ACTGTCTTTT TGGATAGCCT CAATTCCTGA AAAAACTAGA TTTGGAGAAT AATGCACTAT TGTCCTGGTG TTATAGGCAC TGTCCTTTGT ATTTTTGACT GTGAGGCTAA CGTTGAACTT ATCATTCTGG GATCGGACAA TCAGCAGGTC CTTTTCAGTG GTGGCGACAT GCAGGCTGAG GTCTGAGATA CATTTTTCCT TATTTCCACA ATCTTTGGCA AAGGGAATAT ATTCATGTAC TGAGTTTGGT AGAGAATCAT CAAGAACAGG CCCATTTTCT GGATCGGTAA GATTAAAGTC CAACGTTATT CTCACAGAGT CCTGAAAGTC ATGCTTGTCC AACATGTAGA AGGAGTGCTT AGTGCATTCT GATTTTCGAA CTGTGATGTT CCTTTGAACC TTTCTCTCTT GAGTTCCAGA GAAAAAACTT CGTGATATTT GTCTTAGTGA ATCTAGGGTG ACACGGTACT GCAAATCAGC TTCATAAATC GTGTCTTCTT TAGACTTTAA TTTCACATCA AAACACACTG TAGCATTTAT GCATACTGTT TCCTTTCCCT CCATATGGCA GTTTTTCTTT TGAATATTCA CTTTATTTGG CTCAAAATTC ATGGTCACTT TAACTACGGC CACATCTCGG GACCAGAAGA GGGCAGCACC ACCAAGGCCC CCAATAGTCA CATCTGTCAG ACCGTCACCA TTTAAATCCA TTTCTCCGTG GATAGACTGG CCAAAAAATT TCAGTGTCTT ACCATCCCCA CCTGATGGAA TACGTTGTGC ATACTCTTTC CTTATAGTCT TGCCACTTCC ATGATAAATG TACACAGCTC CCCCGTGATC ATCTTCCAGC GGAGCTCCTA TCACGATGTC ATTAAATCCA TCAAGATTGA GGTCTTTTAC AGCAGCAATT GCAGTTCCAA AACGAGCCCC GCATGGCTCA TTTTTGTTTT CTGTTGTGCA TGAATTGTGC TGCCGAGATG AACAGCACGT CTGCTTAATA GGTTCCAGGC TCATTTGATA TTCAAACCTT GTCTGATTGA GAGCATACAC ATACACTTTT CCTTGCTCCT CCTTCTCTGT TCCCATGTAC ATAGGGGCTC CGACTAGAAG AATGTCAGTA TTAGAATCCT TGTCAATGTC AGTTGTTGTT AAAATACTGC CAAAGTAGGA ACCAATCTGT TCTCCACTGA GCGTCTGGAG AATTTTGATG TTTCCATCTT CCATCCTGTA GATAATGACC TGGCCTGTAT GATTGTACCG AGGCTGTCCA GCAATATAGA GCACATCTCC AGAAGAAGCA GTAGCAGAGT TTACAGTGTA ACCTAAATAA GAAGCAAGCG GTTCATTCTT TTTGGTAGAC TCAACATTAA AGGTTGTGTT TCGAGGGATT ATGATTTGAC TAGCCTTCTG CATGACAACT GTTCCATTCC AATCATAGGC TCCTACTGCT CCAAGCATGA CCCAGTCCTG TGAATAATGA GCACTGAAGC CAGTCTGAGA CATTTCCATT TCAAATGAAG CTGCTGACTG GTCAGCTGTG GCTTCCAGGG CAAATATTCT TTCTCCCAGA GTTTTAACAA TGGTGACTAG AGCCAATTCA TCAGAGACAT TGAAGAAATG CTTTTCAGTG GGTTCACTTG CAATTGATTT TATTTCCTCC ACAAATTTTT CAGTGCTTAA ATTTCCTCGG TTATAGCTGC CAAGAATAGC TATGGAAAAC CGTTGAATGT TTTCATCTTC ACAGTCTTGG ATGACCTTCT TCAGTCGATG ATTGTCATGA GACTCTCCAT CTGTCACAAT AACCATGACT TTTTTAACTC CTCTTCGGGC ACCCCGGGCT TCCGTGAATG CCTCCTTTCT TGCTGTGTCT ATTCCAAGAG CTGTCATAGT CTGGCGGCCA CCTCTCTGGA CTATTTTCTT TGCTGCAACA AGTACCTCTT CGGTGGAAGA ATACTTATTG AGGTTGAACT CATGGGTCAC GTTTTCTCCA TACTGTACAA TTCCAACCTG TGTCTGTTTA GGACCAATAT CCATTCTTTC AAGAAGGTCA TTTAAAAAAG CTGTAACACT GTCCCATGGG TAAATACTGT TGGAACCATC CAGCACTATG ACTATGTCCA GTTGAGTGCT GCATTCTTGT ACAGGGGCAA TGGAATTCAC GACTTGAAAT GTGGGGCTGA CGTCAGAACA GATTCCAGTT GTGTAATGCA AATGTCCACA TCTATAGGCA TATAAGGGCC CACAAGCCAG AAATCCTCCA TTTGGGTTGG TGACTAAAGT TGATCCAAAT GTCATGTTCT CCTTTACTTC TGTGACATTG GGAATTGATG TATTAACTGG TAGATCCAAC TTTACGCAAG GTAATGATTC ACCTCTCCCA ACTGGACACT TATAGACATC TCCAGTTCTG TTTTTGGGTT GGCCAACTAA CGGAGAACCA ATAAGCACCC ATTTTCCTTC TTCATTTTCA TATTGTTGAA CAGTATATCC AAACATGTCT TCCACCGGGC CGCTGAAAGT CATTGAATTT TTCACATCAA CATTGAATGA TACGCAGCAG CGTAGAACAA CAGTGAGGAG CCAGCAGCAG GCGACAGCGA CCCCTGGGCG GGCGCGGGGC CGAGGGGCCA TGGCCGGAGC AGCCTCAGCG CCAGGGGGCC GCGCTGGTTC GCAGGGCAGG ACAAGGGCGA ATTCTGCAGA TATCCATCAC ACTGGCGGCC GCTGGAGCAT GCATCTAGAG GCCC
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