pBiT1.1-C-TK-LgBiT-ACTN4
$113.00
In stock
SKU
PL75733
NM_004924.6;ACTININ-4;FSGS;FSGS1
100μg liquid plasmid(Instantly):$134
| name | pBiT1.1-C-TK-LgBiT-ACTN4 | another name | apBiT1.1-C-TK-LgBiT-ACTN4 |
| Insert Size (bp) | 2733bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | TK | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CGTTAAGGTC ACTTCGCATA TTAAGGTGAC GCGTGTGGCC TCGAACACCG AGCGACCCTG CAGCGACCCG CTTAAAAGCT TGGCAATCCG GTACTGTTGG TAAAGCCACC AGATCTGCTA GCGATCGCCT AAGTGGGAGC TCAGGGGAAT TCGCCACCAT GGTGGACTAC CACGCGGCGA ACCAGTCGTA CCAGTACGGC CCCAGCAGCG CGGGCAATGG CGCTGGCGGC GGGGGCAGCA TGGGCGACTA CATGGCCCAG GAGGACGACT GGGACCGGGA CCTGCTGCTG GACCCGGCCT GGGAGAAGCA GCAGCGCAAG ACCTTCACGG CATGGTGCAA CTCCCACCTG CGGAAGGCAG GCACACAGAT CGAGAACATT GATGAGGACT TCCGAGACGG GCTCAAGCTC ATGCTGCTCC TGGAGGTCAT ATCAGGGGAG CGGTTACCTA AGCCGGAGCG GGGGAAGATG AGAGTGCACA AAATCAACAA TGTGAACAAA GCGCTGGACT TTATTGCCAG CAAAGGCGTC AAGCTGGTCT CCATCGGGGC AGAAGAGATT GTGGACGGCA ACGCAAAGAT GACCCTGGGA ATGATCTGGA CCATCATCCT TAGGTTCGCC ATCCAGGACA TCTCCGTGGA AGAGACCTCG GCCAAGGAAG GGCTCCTTCT CTGGTGCCAG AGAAAGACAG CCCCGTATAA GAACGTCAAT GTGCAGAACT TCCACATCAG CTGGAAGGAT GGTCTTGCCT TCAATGCCCT GATCCACCGG CACAGACCAG AGCTGATTGA GTATGACAAG CTGAGGAAGG ACGACCCTGT CACCAACCTG AACAATGCCT TCGAAGTGGC TGAGAAATAC CTCGACATCC CCAAGATGCT GGATGCAGAG GACATCGTGA ACACGGCCCG GCCCGACGAG AAGGCCATAA TGACCTATGT GTCCAGCTTC TACCATGCCT TTTCAGGAGC GCAGAAGGCT GAAACTGCCG CCAACCGGAT CTGTAAGGTG CTGGCTGTCA ACCAAGAGAA CGAGCACCTG ATGGAGGACT ACGAGAAGCT GGCCAGCGAC CTCCTGGAGT GGATCCGGCG CACCATCCCC TGGCTGGAGG ACCGTGTGCC CCAAAAGACT ATCCAGGAGA TGCAGCAGAA GCTGGAGGAC TTCCGCGACT ACCGGCGTGT GCACAAGCCG CCCAAGGTGC AGGAGAAGTG CCAGCTGGAG ATCAACTTCA ACACGCTGCA GACCAAGCTG CGCCTCAGCA ACCGGCCCGC CTTCATGCCC TCCGAGGGCA AGATGGTCTC GGACATCAAC AATGGCTGGC AGCACTTGGA GCAGGCTGAG AAGGGCTACG AGGAGTGGCT GCTGAATGAG ATCCGCAGGC TGGAGCGGCT CGACCACCTG GCAGAGAAGT TCCGGCAGAA GGCCTCCATC CACGAGGCCT GGACTGACGG GAAGGAAGCC ATGCTGAAGC ACCGGGACTA CGAGACGGCC ACACTATCGG ACATCAAAGC CCTCATTCGC AAGCACGAGG CCTTCGAGAG CGACCTGGCT GCGCACCAGG ACCGCGTGGA GCAGATCGCC GCCATTGCCC AGGAGCTCAA CGAGCTGGAT TACTACGACT CCCACAATGT CAACACCCGG TGCCAGAAGA TCTGTGACCA GTGGGACGCC CTCGGCTCTC TGACACATAG TCGCAGGGAA GCCCTGGAGA AAACAGAGAA GCAGCTGGAG GCCATCGACC AGCTGCACCT GGAATACGCC AAGCGCGCGG CCCCCTTCAA CAACTGGATG GAGAGCGCCA TGGAGGACCT CCAGGACATG TTCATCGTCC ATACCATCGA GGAGATTGAG GGCCTGATCT CAGCCCATGA CCAGTTCAAG TCCACCCTGC CGGACGCCGA TAGGGAGCGC GAGGCCATCC TGGCCATCCA CAAGGAGGCC CAGAGGATCG CTGAGAGCAA CCACATCAAG CTGTCGGGCA GCAACCCCTA CACCACCGTC ACCCCGCAAA TCATCAACTC CAAGTGGGAG AAGGTGCAGC AGCTGGTGCC AAAACGGGAC CATGCCCTCC TGGAGGAGCA GAGCAAGCAG CAGTCCAACG AGCACCTGCG CCGCCAGTTC GCCAGCCAGG CCAATGTTGT GGGGCCCTGG ATCCAGACCA AGATGGAGGA GATCGGGCGC ATCTCCATTG AGATGAACGG GACCCTGGAG GACCAGCTGA GCCACCTGAA GCAGTATGAA CGCAGCATCG TGGACTACAA GCCCAACCTG GACCTGCTGG AGCAGCAGCA CCAGCTCATC CAGGAGGCCC TCATCTTCGA CAACAAGCAC ACCAACTATA CCATGGAGCA CATCCGCGTG GGCTGGGAGC AGCTGCTCAC CACCATTGCC CGCACCATCA ACGAGGTGGA GAACCAGATC CTCACCCGCG ACGCCAAGGG CATCAGCCAG GAGCAGATGC AGGAGTTCCG GGCGTCCTTC AACCACTTCG ACAAGGATCA TGGCGGGGCG CTGGGGCCCG AGGAGTTCAA GGCCTGCCTC ATCAGCCTGG GCTACGACGT GGAGAACGAC CGGCAGGGTG AGGCCGAGTT CAACCGCATC ATGAGCCTGG TCGACCCCAA CCATAGCGGC CTTGTGACCT TCCAAGCCTT CATCGACTTC ATGTCGCGGG AGACCACCGA CACGGACACG GCTGACCAGG TCATCGCTTC CTTCAAGGTC TTAGCAGGGG ACAAGAACTT CATCACAGCT GAGGAGCTGC GGAGAGAGCT GCCCCCCGAC CAGGCCGAGT ACTGCATCGC CCGCATGGCG CCATACCAGG GCCCTGACGC CGTGCCCGGT GCCCTCGACT ACAAGTCCTT CTCCACGGCC TTGTATGGCG AGAGCGACCT GCTCTCGAGC GGTGGTGGCG GGAGCGGAGG TGGAGGGTCG TCAGGTGTCT TCACACTCGA AGATTTCGTT GGGGACTGGG AACAGACAGC CGCCTACAAC CTGGACCAAG TCCTTGAACA GGGAGGTGTG TCCAGTTTGC TGCAGAATCT CGCCGTGTCC GTAACTCCGA TCCAAAGGAT TGTCCGGAGC GGTGAAAATG CCCTGAAGAT CGACATCCAT GTCATCATCC CGTATGAAGG TCTGAGCGCC GACCAAATGG CCCAGATCGA AGAGGTGTTT AAGGTGGTGT ACCCTGTGGA TGATCATCAC TTTAAGGTGA TCCTGCCCTA TGGCACACTG GTAATCGACG GGGTTACGCC GAACATGCTG AACTATTTCG GACGGCCGTA TGAAGGCATC GCCGTGTTCG ACGGCAAAAA GATCACTGTA ACAGGGACCC TGTGGAACGG CAACAAAATT ATCGACGAGC GCCTGATCAC CCCCGACGGC TCCATGCTGT TCCGAGTAAC CATCAACAGC TAATCTAGAG TCGGGCGCCG CCGCTCAGG
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
