pBinGFP2-信号肽-基因-1
$128.00
In stock
SKU
PL75632
100μg liquid plasmid(Instantly):$163
| name | pBinGFP2-信号肽-基因-1 | another name | apBinGFP2-信号肽-基因-1 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | 35s | Replicon | OriV |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTGTGAACCA TCACCCAAAT CAAGTTTTTT GGGGTCGAGG TGCCGTAAAG CACTAAATCG GAACCCTAAA GGGAGCCCCC GATTTAGAGC TTGACGGGGA AAGCCGGCGA ACGTGGCGAG AAAGGAAGGG AAGAAAGCGA AAGGAGCGGG CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGAATTGTT AATTAAGAAT TCGAGCTCCT TGCATGCCTG CAGGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATAACATGG TGGAGCACGA CACACTTGTC TACTCCAAAA ATATCAAAGA TACAGTCTCA GAAGACCAAA GGGCAATTGA GACTTTTCAA CAAAGGGTAA TATCCGGAAA CCTCCTCGGA TTCCATTGCC CAGCTATCTG TCACTTTATT GTGAAGATAG TGGAAAAGGA AGGTGGCTCC TACAAATGCC ATCATTGCGA TAAAGGAAAG GCCATCGTTG AAGATGCCTC TGCCGACAGT GGTCCCAAAG ATGGACCCCC ACCCACGAGG AGCATCGTGG AAAAAGAAGA CGTTCCAACC ACGTCTTCAA AGCAAGTGGA TTGATGTGAT ATCTCCACTG ACGTAAGGGA TGACGCACAA TCCCACTATC CTTCGCAAGA CCCTTCCTCT ATATAAGGAA GTTCATTTCA TTTGGAGAGG ACCTCGAGAA TTCTCAACAC AACATATACA AAACAAACGA ATCTCAAGCA ATCAAGCATT CTACTTCTAT TGCAGCAATT TAAATCATTT CTTTTAAAGC AAAAGCAATT TTCTGAAAAT TTTCACCATT TACGAACGAT AGCCATGAGA CTTTTCGCTG TTGCTGCTGC GGCTGCAGTG GCGAGTGCAG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGGGTACCA TGACTCCGTC GAACAACTGG AACCAGACCG CCGATATTGT TAATGGCACC ATTCGCATCT ACGGAGACGA TGCCTATCCC GTGCAGTACC GCCGTACCAT GCTAAACAAC GCTCCACGTG CCCGCTATCC CGGTTTCAAA CAGGAGACAT TGATTCTCAA GAACGGAACC ATCCGCCGCG AGGGTGCCAA GGTCTTGCCC TGCGACATCC AGTTCGAACG CGACCAACCA ATGACCCTCC GCGACGGCAC CGTCATCTAC ACTGACATCT TCCGGCCCGT GGGAAGCGGC GCGAACAACA CCGTCCCAGC CATCGTGGCC CACAGTCCTT ACGGCAAGGA GGTTGGTGGA CAATGGCTGG ATGATGTGCC CGAACGCTCC GGCGTTCCCC TGTCTTTGGT CTCAGAGCTC CAAAAGTTTG AAGGCCCCGA TCCGGCACAC TGGGTCTGTC AGGGATACGC CATTCTCAAT CCCGATGCCC GCGGTGCCTA TTCCTCGGAG GGCAACATCA CCTTCTGGGG TCGTCAGCTT GCTGAGGACG GCTATGACTT CATCGAGTGG GTAGCCGACC AGGCCTGGAG CTCGGGCAAG GTTGGCATGT CGGGCAACTC TTGGCTTTCA GTCTCGCAGT GGTTCATCGC CGCCGAAAAC CCCCCTCATC TGACCGCGAT CGCCCCTTGG GAAGGTCTGA CGGACGTGTA CCGTGACTCG ACCAACCGCG GTGGTTTCCC CGCCCCAGCC TTTCAAGAGG AGATCATGAC CACCTTCGCC GGCGAAAACT ATCTAGAGGA CAATGCCCGC AAGATCTACA GCGAGCCTTT TATCAACATC TACTGGGAGG ATAAGATAGC TCGCGTCGAG AACATCACTG TCCCTGCCTA CGTTGTGGCC AGTTATACGA ATGCCGTGCA CACTCGCTAG GGATCCTCTA GATGAACTAG AGTCCGCAAA AATCACCAGT CTCTCTCTAC AAATCTATCT CTCTCTATTT TTCTCCAGAA TAATGTGTGA GTAGTTCCCA GATAAGGGAA TTAGGGTTCT TATAGGGTTT CGCTCATGTG TTGAGCATAT AAGAAACCCT TAGTATGTAT TTGTATTTGT AAAATACTTC TATCAATAAA ATTTCTAATT CCTAAAACCA AAATCCAGTG ACAAGCTTGG CGCGCCAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CCAAAGACAA AAGGGCGACA TTCAACCGAT TGAGGGAGGG AAGGTAAATA TTGACGGAAA TTATTCATTA AAGGTGAATT ATCACCGTCA CCGACTTGAG CCATTTGGGA ATTAGAGCCA GCAAAATCAC CAGTAGCACC ATTACCATTA GCAAGGCCGG AAACGTCACC AATGAAACCA TCGATAGCAG CACCGTAATC AGTAGCGACA GAATCAAGTT TGCCTTTAGC GTCAGACTGT AGCGCGTTTT CATCGGCATT TTCGGTCATA GCCCCCTTAT TAGCGTTTGC CATCTTTTCA TAATCAAAAT CACCGGAACC AGAGCCACCA CCGGAACCGC CTCCCTCAGA GCCGCCACCC TCAGAACCGC CACCCTCAGA GCCACCACCC TCAGAGCCGC CACCAGAACC ACCACCAGAG CCGCCGCCAG CATTGACAGG AGGCCCGATC TAGTAACATA GATGACACCG CGCGCGATAA TTTATCCTAG TTTGCGCGCT ATATTTTGTT TTCTATCGCG TATTAAATGT ATAATTGCGG GACTCTAATC ATAAAAACCC ATCTCATAAA TAACGTCATG CATTACATGT TAATTATTAC ATGCTTAACG TAATTCAACA GAAATTATAT GATAATCATC GCAAGACCGG CAACAGGATT CAATCTTAAG AAACTTTATT GCCAAATGTT TGAACGATCG GGGATCATCC GGGTCTGTGG CGGGAACTCC ACGAAAATAT CCGAACGCAG CAAGATATCG CGGTGCATCT CGGTCTTGCC TGGGCAGTCG CCGCCGACGC CGTTGATGTG GACGCCGGGC CCGATCATAT TGTCGCTCAG GATCGTGGCG TTGTGCTTGT CGGCCGTTGC TGTCGTAATG ATATCGGCAC CTTCGACCGC CTGTTCCGCA GAGATCCCGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGGTCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCATC GCCGTCGGGC ATGCGCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC CTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCAGATC CGGTGCAGAT TATTTGGATT GAGAGTGAAT ATGAGACTCT AATTGGATAC CGAGGGGAAT TTATGGAACG TCAGTGGAGC ATTTTTGACA AGAAATATTT GCTAGCTGAT AGTGACCTTA GGCGACTTTT GAACGCGCAA TAATGGTTTC TGACGTATGT GCTTAGCTCA TTAAACTCCA GAAACCCGCG GCTGAGTGGC TCCTTCAACG TTGCGGTTCT GTCAGTTCCA AACGTAAAAC GGCTTGTCCC GCGTCATCGG CGGGGGTCAT AACGTGACTC CCTTAATTCT CCGCTCATGA TCAGATTGTC GTTTCCCGCC TTCAGTTTGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT CTCACTGGTG AAAAGAAAAA CCACCCCAGT ACATTAAAAA CGTCCGCAAT GTGTTATTAA GTTGTCTAAG CGTCAATTTG TTTACACCAC AATATATCCT GCCACCAGCC AGCCAACAGC TCCCCGACCG GCAGCTCGGC ACAAAATCAC CACTCGATAC AGGCAGCCCA TCAGTCCGGG ACGGCGTCAG CGGGAGAGCC GTTGTAAGGC GGCAGACTTT GCTCATGTTA CCGATGCTAT TCGGAAGAAC GGCAACTAAG CTGCCGGGTT TGAAACACGG ATGATCTCGC GGAGGGTAGC ATGTTGATTG TAACGATGAC AGAGCGTTGC TGCCTGTGAT CAAATATCAT CTCCCTCGCA GAGATCCGAA TTATCAGCCT TCTTATTCAT TTCTCGCTTA ACCGTGACAG GCTGTCGATC TTGAGAACTA TGCCGACATA ATAGGAAATC GCTGGATAAA GCCGCTGAGG AAGCTGAGTG GCGCTATTTC TTTAGAAGTG AACGTTGACG ATATCAACTC CCCTATCCAT TGCTCACCGA ATGGTACAGG TCGGGGACCC GAAGTTCCGA CTGTCGGCCT GATGCATCCC CGGCTGATCG ACCCCAGATC TGGGGCTGAG AAAGCCCAGT AAGGAAACAA CTGTAGGTTC GAGTCGCGAG ATCCCCCGGA ACCAAAGGAA GTAGGTTAAA CCCGCTCCGA TCAGGCCGAG CCACGCCAGG CCGAGAACAT TGGTTCCTGT AGGCATCGGG ATTGGCGGAT CAAACACTAA AGCTACTGGA ACGAGCAGAA GTCCTCCGGC CGCCAGTTGC CAGGCGGTAA AGGTGAGCAG AGGCACGGGA GGTTGCCACT TGCGGGTCAG CACGGTTCCG AACGCCATGG AAACCGCCCC CGCCAGGCCC GCTGCGACGC CGACAGGATC TAGCGCTGCG TTTGGTGTCA ACACCAACAG CGCCACGCCC GCAGTTCCGC AAATAGCCCC CAGGACCGCC ATCAATCGTA TCGGGCTACC TAGCAGAGCG GCAGAGATGA ACACGACCAT CAGCGGCTGC ACAGCGCCTA CCGTCGCCGC GACCCCGCCC GGCAGGCGGT AGACCGAAAT AAACAACAAG CTCCAGAATA GCGAAATATT AAGTGCGCCG AGGATGAAGA TGCGCATCCA CCAGATTCCC GTTGGAATCT GTCGGACGAT CATCACGAGC AATAAACCCG CCGGCAACGC CCGCAGCAGC ATACCGGCGA CCCCTCGGCC TCGCTGTTCG GGCTCCACGA AAACGCCGGA CAGATGCGCC TTGTGAGCGT CCTTGGGGCC GTCCTCCTGT TTGAAGACCG ACAGCCCAAT GATCTCGCCG TCGATGTAGG CGCCGAATGC CACGGCATCT CGCAACCGTT CAGCGAACGC CTCCATGGGC TTTTTCTCCT CGTGCTCGTA AACGGACCCG AACATCTCTG GAGCTTTCTT CAGGGCCGAC AATCGGATCT CGCGGAAATC CTGCACGTCG GCCGCTCCAA GCCGTCGAAT CTGAGCCTTA ATCACAATTG TCAATTTTAA TCCTCTGTTT ATCGGCAGTT CGTAGAGCGC GCCGTGCGTC CCGAGCGATA CTGAGCGAAG CAAGTGCGTC GAGCAGTGCC CGCTTGTTCC TGAAATGCCA GTAAAGCGCT GGCTGCTGAA CCCCCAGCCG GAACTGACCC CACAAGGCCC TAGCGTTTGC AATGCACCAG GTCATCATTG ACCCAGGCGT GTTCCACCAG GCCGCTGCCT CGCAACTCTT CGCAGGCTTC GCCGACCTGC TCGCGCCACT TCTTCACGCG GGTGGAATCC GATCCGCACA TGAGGCGGAA GGTTTCCAGC TTGAGCGGGT ACGGCTCCCG GTGCGAGCTG AAATAGTCGA ACATCCGTCG GGCCGTCGGC GACAGCTTGC GGTACTTCTC CCATATGAAT TTCGTGTAGT GGTCGCCAGC AAACAGCACG ACGATTTCCT CGTCGATCAG GACCTGGCAA CGGGACGTTT TCTTGCCACG GTCCAGGACG CGGAAGCGGT GCAGCAGCGA CACCGATTCC AGGTGCCCAA CGCGGTCGGA CGTGAAGCCC ATCGCCGTCG CCTGTAGGCG CGACAGGCAT TCCTCGGCCT TCGTGTAATA CCGGCCATTG ATCGACCAGC CCAGGTCCTG GCAAAGCTCG TAGAACGTGA AGGTGATCGG CTCGCCGATA GGGGTGCGCT TCGCGTACTC CAACACCTGC TGCCACACCA GTTCGTCATC GTCGGCCCGC AGCTCGACGC CGGTGTAGGT GATCTTCACG TCCTTGTTGA CGTGGAAAAT GACCTTGTTT TGCAGCGCCT CGCGCGGGAT TTTCTTGTTG CGCGTGGTGA ACAGGGCAGA GCGGGCCGTG TCGTTTGGCA TCGCTCGCAT CGTGTCCGGC CACGGCGCAA TATCGAACAA GGAAAGCTGC ATTTCCTTGA TCTGCTGCTT CGTGTGTTTC AGCAACGCGG CCTGCTTGGC CTCGCTGACC TGTTTTGCCA GGTCCTCGCC GGCGGTTTTT CGCTTCTTGG TCGTCATAGT TCCTCGCGTG TCGATGGTCA TCGACTTCGC CAAACCTGCC GCCTCCTGTT CGAGACGACG CGAACGCTCC ACGGCGGCCG ATGGCGCGGG CAGGGCAGGG GGAGCCAGTT GCACGCTGTC GCGCTCGATC TTGGCCGTAG CTTGCTGGAC CATCGAGCCG ACGGACTGGA AGGTTTCGCG GGGCGCACGC ATGACGGTGC GGCTTGCGAT GGTTTCGGCA TCCTCGGCGG AAAACCCCGC GTCGATCAGT TCTTGCCTGT ATGCCTTCCG GTCAAACGTC CGATTCATTC ACCCTCCTTG CGGGATTGCC CCGACTCACG CCGGGGCAAT GTGCCCTTAT TCCTGATTTG ACCCGCCTGG TGCCTTGGTG TCCAGATAAT CCACCTTATC GGCAATGAAG TCGGTCCCGT AGACCGTCTG GCCGTCCTTC TCGTACTTGG TATTCCGAAT CTTGCCCTGC ACGAATACCA GCGACCCCTT GCCCAAATAC TTGCCGTGGG CCTCGGCCTG AGAGCCAAAA CACTTGATGC GGAAGAAGTC GGTGCGCTCC TGCTTGTCGC CGGCATCGTT GCGCCACATC TAGGTACTAA AACAATTCAT CCAGTAAAAT ATAATATTTT ATTTTCTCCC AATCAGGCTT GATCCCCAGT AAGTCAAAAA ATAGCTCGAC ATACTGTTCT TCCCCGATAT CCTCCCTGAT CGACCGGACG CAGAAGGCAA TGTCATACCA CTTGTCCGCC CTGCCGCTTC TCCCAAGATC AATAAAGCCA CTTACTTTGC CATCTTTCAC AAAGATGTTG CTGTCTCCCA GGTCGCCGTG GGAAAAGACA AGTTCCTCTT CGGGCTTTTC CGTCTTTAAA AAATCATACA GCTCGCGCGG ATCTTTAAAT GGAGTGTCTT CTTCCCAGTT TTCGCAATCC ACATCGGCCA GATCGTTATT CAGTAAGTAA TCCAATTCGG CTAAGCGGCT GTCTAAGCTA TTCGTATAGG GACAATCCGA TATGTCGATG GAGTGAAAGA GCCTGATGCA CTCCGCATAC AGCTCGATAA TCTTTTCAGG GCTTTGTTCA TCTTCATACT CTTCCGAGCA AAGGACGCCA TCGGCCTCAC TCATGAGCAG ATTGCTCCAG CCATCATGCC GTTCAAAGTG CAGGACCTTT GGAACAGGCA GCTTTCCTTC CAGCCATAGC ATCATGTCCT TTTCCCGTTC CACATCATAG GTGGTCCCTT TATACCGGCT GTCCGTCATT TTTAAATATA GGTTTTCATT TTCTCCCACC AGCTTATATA CCTTAGCAGG AGACATTCCT TCCGTATCTT TTACGCAGCG GTATTTTTCG ATCAGTTTTT TCAATTCCGG TGATATTCTC ATTTTAGCCA TTTATTATTT CCTTCCTCTT TTCTACAGTA TTTAAAGATA CCCCAAGAAG CTAATTATAA CAAGACGAAC TCCAATTCAC TGTTCCTTGC ATTCTAAAAC CTTAAATACC AGAAAACAGC TTTTTCAAAG TTGTTTTCAA AGTTGGCGTA TAACATAGTA TCGACGGAGC CGATTTTGAA ACCACAATTA TGGGTGATGC TGCCAACTTA CTGATTTAGT GTATGATGGT GTTTTTGAGG TGCTCCAGTG GCTTCTGTGT CTATCAGCTG TCCCTCCTGT TCAGCTACTG ACGGGGTGGT GCGTAACGGC AAAAGCACCG CCGGACATCA GCGCTATCTC TGCTCTCACT GCCGTAAAAC ATGGCAACTG CAGTTCACTT ACACCGCTTC TCAACCCGGT ACGCACCAGA AAATCATTGA TATGGCCATG AATGGCGTTG GATGCCGGGC AACAGCCCGC ATTATGGGCG TTGGCCTCAA CACGATTTTA CGTCACTTAA AAAACTCAGG CCGCAGTCGG TAACCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATAGTAACC TCGCGCATAC AGCCGGGCAG TGACGTCATC GTCTGCGCGG AAATGGACGA ACAGTGGGGC TATGTCGGGG CTAAATCGCG CCAGCGCTGG CTGTTTTACG CGTATGACAG TCTCCGGAAG ACGGTTGTTG CGCACGTATT CGGTGAACGC ACTATGGCGA CGCTGGGGCG TCTTATGAGC CTGCTGTCAC CCTTTGACGT GGTGATATGG ATGACGGATG GCTGGCCGCT GTATGAATCC CGCCTGAAGG GAAAGCTGCA CGTAATCAGC AAGCGATATA CGCAGCGAAT TGAGCGGCAT AACCTGAATC TGAGGCAGCA CCTGGCACGG CTGGGACGGA AGTCGCTGTC GTTCTCAAAA TCGGTGGAGC TGCATGACAA AGTCATCGGG CATTATCTGA ACATAAAACA CTATCAATAA GTTGGAGTCA TTACCCAATT ATGATAGAAT TTACAAGCTA TAAGGTTATT GTCCTGGGTT TCAAGCATTA GTCCATGCAA GTTTTTATGC TTTGCCCATT CTATAGATAT ATTGATAAGC GCGCTGCCTA TGCCTTGCCC CCTGAAATCC TTACATACGG CGATATCTTC TATATAAAAG ATATATTATC TTATCAGTAT TGTCAATATA TTCAAGGCAA TCTGCCTCCT CATCCTCTTC ATCCTCTTCG TCTTGGTAGC TTTTTAAATA TGGCGCTTCA TAGAGTAATT CTGTAAAGGT CCAATTCTCG TTTTCATACC TCGGTATAAT CTTACCTATC ACCTCAAATG GTTCGCTGGG TTTATCGCAC CCCCGAACAC GAGCACGGCA CCCGCGACCA CTATGCCAAG AATGCCCAAG GTAAAAATTG CCGGCCCCGC CATGAAGTCC GTGAATGCCC CGACGGCCGA AGTGAAGGGC AGGCCGCCAC CCAGGCCGCC GCCCTCACTG CCCGGCACCT GGTCGCTGAA TGTCGATGCC AGCACCTGCG GCACGTCAAT GCTTCCGGGC GTCGCGCTCG GGCTGATCGC CCATCCCGTT ACTGCCCCGA TCCCGGCAAT GGCAAGGACT GCCAGCGCTG CCATTTTTGG GGTGAGGCCG TTCGCGGCCG AGGGGCGCAG CCCCTGGGGG GATGGGAGGC CCGCGTTAGC GGGCCGGGAG GGTTCGAGAA GGGGGGGCAC CCCCCTTCGG CGTGCGCGGT CACGCGCACA GGGCGCAGCC CTGGTTAAAA ACAAGGTTTA TAAATATTGG TTTAAAAGCA GGTTAAAAGA CAGGTTAGCG GTGGCCGAAA AACGGGCGGA AACCCTTGCA AATGCTGGAT TTTCTGCCTG TGGACAGCCC CTCAAATGTC AATAGGTGCG CCCCTCATCT GTCAGCACTC TGCCCCTCAA GTGTCAAGGA TCGCGCCCCT CATCTGTCAG TAGTCGCGCC CCTCAAGTGT CAATACCGCA GGGCACTTAT CCCCAGGCTT GTCCACATCA TCTGTGGGAA ACTCGCGTAA AATCAGGCGT TTTCGCCGAT TTGCGAGGCT GGCCAGCTCC ACGTCGCCGG CCGAAATCGA GCCTGCCCCT CATCTGTCAA CGCCGCGCCG GGTGAGTCGG CCCCTCAAGT GTCAACGTCC GCCCCTCATC TGTCAGTGAG GGCCAAGTTT TCCGCGAGGT ATCCACAACG CCGGCGGCCG CGGTGTCTCG CACACGGCTT CGACGGCGTT TCTGGCGCGT TTGCAGGGCC ATAGACGGCC GCCAGCCCAG CGGCGAGGGC AACCAGCCCG G
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