pMV261-6×His
$128.00
In stock
SKU
PL75443
100μg liquid plasmid(Instantly):$163
| name | pMV261-6×His | another name | apMV261-6×His |
| Insert Size (bp) | Plasmid host | Mycobacterium tuberculosis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | HSP60 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCTAGCCAAC AAAGCGACGT TGTGTCTCAA AATCTCTGAT GTTACATTGC ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC AAGGGGTGTT ATGAGCCATA TTCAACGGGA AACGTCTTGC TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CCATGCGCCA GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGGA AAACAGCATT CCAGGTATTA GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA ATCTTTTGCC ATTCTCACCG GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGGCT TTGTTGAATA AATCGAACTT TTGCTGAGTT GAAGGATCAG ATCACGCATC TTCCCGACAA CGCAGACCGT TCCGTGGCAA AGCAAAAGTT CAAAATCACC AACTGGTCCA CCTACAACAA AGCTCTCATC AACCGTGGCT CCCTCACTTT CTGGCTGGAT GATGGGGCGA TTCAGGCCTG GTATGAGTCA GCAACACCTT CTTCACGAGG CAGACCTCAC TAGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCA TTGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA ACGCGTGAGC CCACCAGCTC CGTAAGTTCG GGTGCTGTGT GGCTCGTACC CGCGCATTCA GGCGGCAGGG GGTCTAACGG GTCTAAGGCG GCGTGTACGG CCGCCACAGC GGCTCTTAGC GGCCCGGAAA CGTCCTCGAA ACGACGCATG TGTTCCTCCT GGTTGGTACA GGTGGTTGGG GGTGCTCGGC TGTCGCTGGT GTTTCATCAT CAGGGCTCGA CGGGAGAGCG GGGGAGTGTG CAGTTGTGGG GTGGCCCCTC AGCGAAATAT CTGACTTGGA GCTCGTGTCG GACCATACAC CGGTGATTAA TCGTGGTTTA TTATCAAGCG TGAGCCACGT CGCCGACGAA TTTGAGCAGC TCTGGCTGCC GTACTGGTCC CTGGCAAGCG ACGATCTGCT CGAGGGGATC TACCGCCAAA GCCGCGCGTC GGCCCTAGGC CGCCGGTACA TCGAGGCGAA CCCAACAGCG CTGGCAAACC TGCTGGTCGT GGACGTAGAC CATCCAGACG CAGCGCTCCG AGCGCTCAGC GCCCGGGGGT CCCATCCGCT GCCCAACGCG ATCGTGGGCA ATCGCGCCAA CGGCCACGCA CACGCAGTGT GGGCACTCAA CGCCCCTGTT CCACGCACCG AATACGCGCG GCGTAAGCCG CTCGCATACA TGGCGGCGTG CGCCGAAGGC CTTCGGCGCG CCGTCGATGG CGACCGCAGT TACTCAGGCC TCATGACCAA AAACCCCGGC CACATCGCCT GGGAAACGGA ATGGCTCCAC TCAGATCTCT ACACACTCAG CCACATCGAG GCCGAGCTCG GCGCGAACAT GCCACCGCCG CGCTGGCGTC AGCAGACCAC GTACAAAGCG GCTCCGACGC CGCTAGGGCG GAATTGCGCA CTGTTCGATT CCGTCAGGTT GTGGGCCTAT CTTCCCGCCC TCATGCGGAT CTACCTGCCG ACCCGGAACG TGGACGGACT CGGCCGCGCG ATCTATGCCG AGTGCCACGC GCGAAACGCC GAATTTCCGT GCAACGACGT GTGTCCCGGA CCGCTACCGG ACAGCGAGGT CCGCGCCATC GCCAACAGCA TTTGGCGTTG GATCACAACC AAGTCGCGCA TTTGGGCGGA CGGGATCGTG GTCTACGAGG CCACACTCAG TGCGCGCCAT GCGGCCATCT CGCGGAAGGG CGCAGCAGCG CGCACGGCGG CGAGCACAGT TGCGCGGCGC GCAAAGTCCG CGTCAGCCAT GGAGGCATTG CTATGAGCGA CGGCTACAGC GACGGCTACA GCGACGGCTA CAACTGGCAG CCGACTGTCC GCAAAAAGCG GCGCGTGACC GCCGCCGAAG GCGCTCGAAT CACCGGACTA TCCGAACGCC ACGTCGTCCG GCTCGTGGCG CAGGAACGCA GCGAGTGGTT CGCCGAGCAG GCTGCACGCC GCGAACGCAT CCGCGCCTAT CACGACGACG AGGGCCACTC TTGGCCGCAA ACGGCCAAAC ATTTCGGGCT GCATCTGGAC ACCGTTAAGC GACTCGGCTA TCGGGCGAGG AAAGAGCGTG CGGCAGAACA GGAAGCGGCT CAAAAGGCCC ACAACGAAGC CGACAATCCA CCGCTGTTCT AACGCAATTG GGGAGCGGGT GTCGCGGGGG TTCCGTGGGG GGTTCCGTTG CAACGGGTCG GACAGGTAAA AGTCCTGGTA GACGCTAGTT TTCTGGTTTG GGCCATGCCT GTCTCGTTGC GTGTTTCGTT GCGTCCGTTT TGAATACCAG CCAGACGAGA CGGGGTTCTA CGAATCTTGG TCGATACCAA GCCATTTCCG CTGAATATCG TGGAGCTCAC CGCCAGAATC GGTGGTTGTG GTGATGTACG TGGCGAACTC CGTTGTAGTG CTTGTGGTGG CATCCGTGGC GCGGCCGCGG TACCAGATCT TTAAATCTAG AGGTGACCAC AACGACGCGC CCGCTTTGAT CGGGGACGTC TGCGGCCGAC CATTTACGGG TCTTGTTGTC GTTGGCGGTC ATGGGCCGAA CATACTCACC CGGATCGGAG GGCCGAGGAC AAGGTCGAAC GAGGGGCATG ACCCGGTGCG GGGCTTCTTG CACTCGGCAT AGGCGAGTGC TAAGAATAAC GTTGGCACTC GCGACCGGTG AGTCGTAGGT CGGGACGGTG AGGCCAGGCC CGTCGTCGCA GCGAGTGGCA GCGAGGACAA CTTGAGCCGT CCGTCGCGGG CACTGCGCCC GGCCAGCGTA AGTAGCGGGG TTGCCGTCAC CCGGTGACCC CCGGTTTCAT CCCCGATCCG GAGGAATCAC TTCGCAATGG CCAAGACAAT TGCGGATCCA GCTGCAGAAT TCGAAGCTTA TGCATCATCA CCATCACCAT TGAGTCGACG TAGTTAACTA GCGTACGATC GACTGCCAGG CATCAAATAA AACGAAAGGC TCAGTCGAAA GACTGGGCCT TTCGTTTTAT CTGTTGTTTG TCCGGCCATC ATGGCCGCGG TGATCA
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