pDEST32
$113.00
In stock
SKU
PL75414
100μg liquid plasmid(Instantly):$134
| name | pDEST32 | another name | apDEST32 |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | hybridization | Fragment type | ORF,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Gen,Chl |
| Selective marker | LEU2 | Fluorescent labeling | |
| Promoter | ADH1 | Replicon | pUC |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TATCTCTATG TAAGCTACGT GGCGTGACTT TTGATGAAGC CGCACAAGAG ATACAGGATT GGCAACTGCA AATAGAATCT GGGGATCCCC CCTCGAGATC CGGGATCGAA GAAATGATGG TAAATGAAAT AGGAAATCAA GGAGCATGAA GGCAAAAGAC AAATATAAGG GTCGAACGAA AAATAAAGTG AAAAGTGTTG ATATGATGTA TTTGGCTTTG CGGCGCCGAA AAAACGAGTT TACGCAATTG CACAATCATG CTGACTCTGT GGCGGACCCG CGCTCTTGCC GGCCCGGCGA TAACGCTGGG CGTGAGGCTG TGCCCGGCGG AGTTTTTTGC GCCTGCATTT TCCAAGGTTT ACCCTGCGCT AAGGGGCGAG ATTGGAGAAG CAATAAGAAT GCCGGTTGGG GTTGCGATGA TGACGACCAC GACAACTGGT GTCATTATTT AAGTTGCCGA AAGAACCTGA GTGCATTTGC AACATGAGTA TACTAGAAGA ATGAGCCAAG ACTTGCGAGA CGCGAGTTTG CCGGTGGTGC GAACAATAGA GCGACCATGA CCTTGAAGGT GAGACGCGCA TAACCGCTAG AGTACTTTGA AGAGGAAACA GCAATAGGGT TGCTACCAGT ATAAATAGAC AGGTACATAC AACACTGGAA ATGGTTGTCT GTTTGAGTAC GCTTTCAATT CATTTGGGTG TGCACTTTAT TATGTTACAA TATGGAAGGG AACTTTACAC TTCTCCTATG CACATATATT AATTAAAGTC CAATGCTAGT AGAGAAGGGG GGTAACACCC CTCCGCGCTC TTTTCCGATT TTTTTCTAAA CCGTGGAATA TTTCGGATAT CCTTTTGTTG TTTCCGGGTG TACAATATGG ACTTCCTCTT TTCTGGCAAC CAAACCCATA CATCGGGATT CCTATAATAC CTTCGTTGGT CTCCCTAACA TGTAGGTGGC GGAGGGGAGA TATACAATAG AACAGATACC AGACAAGACA TAATGGGCTA AACAAGACTA CACCAATTAC ACTGCCTCAT TGATGGTGGT ACATAACGAA CTAATACTGT AGCCCTAGAC TTGATAGCCA TCATCATATC GAAGTTTCAC TACCCTTTTT CCATTTGCCA TCTATTGAAG TAATAATAGG CGCATGCAAC TTCTTTTCTT TTTTTTTCTT TTCTCTCTCC CCCGTTGTTG TCTCACCATA TCCGCAATGA CAAAAAAATG ATGGAAGACA CTAAAGGAAA AAATTAACGA CAAAGACAGC ACCAACAGAT GTCGTTGTTC CAGAGCTGAT GAGGGGTATC TCGAAGCACA CGAAACTTTT TCCTTCCTTC ATTCACGCAC ACTACTCTCT AATGAGCAAC GGTATACGGC CTTCCTTCCA GTTACTTGAA TTTGAAATAA AAAAAAGTTT GCTGTCTTGC TATCAAGTAT AAATAGACCT GCAATTATTA ATCTTTTGTT TCCTCGTCAT TGTTCTCGTT CCCTTTCTTC CTTGTTTCTT TTTCTGCACA ATATTTCAAG CTATACCAAG CATACAATCA ACTCCAAGCT TGAAGCAAGC CTCCTGAAAG ATGAAGCTAC TGTCTTCTAT CGAACAAGCA TGCGATATTT GCCGACTTAA AAAGCTCAAG TGCTCCAAAG AAAAACCGAA GTGCGCCAAG TGTCTGAAGA ACAACTGGGA GTGTCGCTAC TCTCCCAAAA CCAAAAGGTC TCCGCTGACT AGGGCACATC TGACAGAAGT GGAATCAAGG CTAGAAAGAC TGGAACAGCT ATTTCTACTG ATTTTTCCTC GAGAAGACCT TGACATGATT TTGAAAATGG ATTCTTTACA GGATATAAAA GCATTGTTAA CAGGATTATT TGTACAAGAT AATGTGAATA AAGATGCCGT CACAGATAGA TTGGCTTCAG TGGAGACTGA TATGCCTCTA ACATTGAGAC AGCATAGAAT AAGTGCGACA TCATCATCGG AAGAGAGTAG TAACAAAGGT CAAAGACAGT TGACTGTATC GTCGAGGTCG AATCAAACAA GTTTGTACAA AAAAGCTGAA CGAGAAACGT AAAATGATAT AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC ACAACATATC CAGTCACTAT GGCGGCCGCG GGTGAGTCAG CATCACCCGA CGCACTTTGC GCCGAATAAA TACCTGTGAC GGAAGATCAC TTCGCAGAAT AAATAAATCC TGGTGTCCCT GTTGATACCG GGAAGCCCTG GGCCAACTTT TGGCGAAAAT GAGACGTTGA TCGGCACGTA AGAGGTTCCA ACTTTCACCA TAATGAAATA AGATCACTAC CGGGCGTATT TTTTGAGTCA TCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG GTTCATCATG CCGTTTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG TACTGCGATG AGTGGCAGGC GGGGCGTAAT CTAGAGGATC CGGCTTACTA AAAGCCAGAT AACAGTATGC GTATTTGCGC GCTGATTTTT GCGGTATAAG AATATATACT GATATGTATA CCCGAAGTAT GTCAAAAAGA GGTATGCTAT GAAGCAGCGT ATTACAGTGA CAGTTGACAG CGACAGCTAT CAGTTGCTCA AGGCATATAT GATGTCAATA TCTCCGGTCT GGTAAGCACA ACCATGCAGA ATGAAGCCCG TCGTCTGCGT GCCGAACGCT GGAAAGCGGA AAATCAGGAA GGGATGGCTG AGGTCGCCCG GTTTATTGAA ATGAACGGCT CTTTTGCTGA CGAGAACAGG GGCTGGTGAA ATGCAGTTTA AGGTTTACAC CTATAAAAGA GAGAGCCGTT ATCGTCTGTT TGTGGATGTA CAGAGTGATA TTATTGACAC GCCCGGGCGA CGGATGGTGA TCCCCCTGGC CAGTGCACGT CTGCTGTCAG ATAAAGTCCC CCGTGAACTT TACCCGGTGG TGCATATCGG GGATGAAAGC TGGCGCATGA TGACCACCGA TATGGCCAGT GTGCCGGTCT CCGTTATCGG GGAAGAAGTG GCTGATCTCA GCCACCGCGA AAATGACATC AAAAACGCCA TTAACCTGAT GTTCTGGGGA ATATAAATGT CAGGCTCCCT TATACACAGC CAGTCTGCAG GTCGACCATA GTGACTGGAT ATGTTGTGTT TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA TCATTTTACG TTTCTCGTTC AGCTTTCTTG TACAAAGTGG TTTGATGGCC GCTAAGTAAG TAAGACGTCG AGCTCTAAGT AAGTAACGGC CGCCACCGCG GTGGAGCTTT GGACTTCTTC GCCAGAGGTT TGGTCAAGTC TCCAATCAAG GTTGTCGGCT TGTCTACCTT GCCAGAAATT TACGAAAAGA TGGAAAAGGG TCAAATCGTT GGTAGATACG TTGTTGACAC TTCTAAATAA GCGAATTTCT TATGATTTAT GATTTTTATT ATTAAATAAG TTATAAAAAA AATAAGTGTA TACAAATTTT AAAGTGACTC TTAGGTTTTA AAACGAAAAT TCTTATTCTT GAGTAACTCT TTCCTGTAGG TCAGGTTGCT TTCTCAGGTA TAGCATGAGG TCGCTCTTAT TGACCACACC TCTACCGGCA TGCCGAGCAA ATGCCTGCAA ATCGCTCCCC ATTTCACCCA ATTGTAGATA TGCTAACTCC AGCAATGAGT TGATGAATCT CGGTGTGTAT TTTATGTCCT CAGAGGACAA CACCTGTTGT AATCGTTCTT CCACACGGAT CCCAATTCGC CCTATAGTGA GTCGTATTAC AATTCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TCGACCGGTC GAGGAGAACT TCTAGTATAT CTACATACCT AATATTATTG CCTTATTAAA AATGGAATCC CAACAATTAC ATCAAAATCC ACATTCTCTT CAAAATCAAT TGTCCTGTAC TTCCTTGTTC ATGTGTGTTC AAAAACGTTA TATTTATAGG ATAATTATAC TCTATTTCTC AACAAGTAAT TGGTTGTTTG GCCGAGCGGT CTAAGGCGCC TGATTCAAGA AATATCTTGA CCGCAGTTAA CTGTGGGAAT ACTCAGGTAT CGTAAGATGC AAGAGTTCGA ATCTCTTAGC AACCATTATT TTTTTCCTCA ACATAACGAG AACACACAGG GGCGCTATCG CACAGAATCA AATTCGATGA CTGGAAATTT TTTGTTAATT TCAGAGGTCG CCTGACGCAT ATACCTTTTT CAACTGAAAA ATTGGGAGAA AAAGGAAAGG TGAGAGCGCC GGAACCGGCT TTTCATATAG AATAGAGAAG CGTTCATGAC TAAATGCTTG CATCACAATA CTTGAAGTTG ACAATATTAT TTAAGGACCT ATTGTTTTTT CCAATAGGTG GTTAGCAATC GTCTTACTTT CTAACTTTTC TTACCTTTTA CATTTCAGCA ATATATATAT ATATATTTCA AGGATATACC ATTCTAATGT CTGCCCCTAA GAAGATCGTC GTTTTGCCAG GTGACCACGT TGGTCAAGAA ATCACAGCCG AAGCCATTAA GGTTCTTAAA GCTATTTCTG ATGTTCGTTC CAATGTCAAG TTCGATTTCG AAAATCATTT AATTGGTGGT GCTGCTATCG ATGCTACAGG TGTTCCACTT CCAGATGAGG CGCTGGAAGC CTCCAAGAAG GCTGATGCCG TTTTGTTAGG TGCTGTGGGT GGTCCTAAAT GGGGTACCGG TAGTGTTAGA CCTGAACAAG GTTTACTAAA AATCCGTAAA GAACTTCAAT TGTACGCCAA CTTAAGACCA TGTAACTTTG CATCCGACTC TCTTTTAGAC TTATCTCCAA TCAAGCCACA ATTTGCTAAA GGTACTGACT TCGTTGTTGT CAGAGAATTA GTGGGAGGTA TTTACTTTGG TAAGAGAAAG GAAGACGATG GTGATGGTGT CGCTTGGGAT AGTGAACAAT ACACCGTTCC AGAAGTGCAA AGAATCACAA GAATGGCCGC TTTCATGGCC CTACAACATG AGCCACCATT GCCTATTTGG TCCTTGGATA AAGCTAATGT TTTGGCCTCT TCAAGATTAT GGAGAAAAAC TGTGGAGGAA ACCATCAAGA ACGAATTCCC TACATTGAAG GTTCAACATC AATTGATTGA TTCTGCCGCC ATGATCCTAG TTAAGAACCC AACCCACCTA AATGGTATTA TAATCACCAG CAACATGTTT GGTGATATCA TCTCCGATGA AGCCTCCGTT ATCCCAGGTT CCTTGGGTTT GTTGCCATCT GCGTCCTTGG CCTCTTTGCC AGACAAGAAC ACCGCATTTG GTTTGTACGA ACCATGCCAC GGTTCTGCTC CAGATTTGCC AAAGAATAAG GTCAACCCTA TCGCCACTAT CTTGTCTGCT GCAATGATGT TGAAATTGTC ATTGAACTTG CCTGAAGAAG GTAAGGCCAT TGAAGATGCA GTTAAAAAGG TTTTGGATGC AGGTATCAGA ACTGGTGATT TAGGTGGTTC CAACAGTACC ACCGAAGTCG GTGATGCTGT CGCCGAAGAA GTTAAGAAAA TCCTTGCTTA AAAAGATTCT CTTTTTTTAT GATATTTGTA CATAAACTTT ATAAATGAAA TTCATAATAG AAACGACACG AAATTACAAA ATGGAATATG TTCATAGGGT AGACGAAACT ATATACGCAA TCTACATACA TTTATCAAGA AGGAGAAAAA GGAGGATGTA AAGGAATACA GGTAAGCAAA TTGATACTAA TGGCTCAACG TGATAAGGAA AAAGAATTGC ACTTTAACAT TAATATTGAC AAGGAGGAGG GCACCACACA AAAAGTTAGG TGTAACAGAA AATCATGAAA CTATGATTCC TAATTTATAT ATTGGAGGAT TTTCTCTAAA AAAAAAAAAA TACAACAAAT AAAAAACACT CAATGACCTG ACCATTTGAT GGAGTTTAAG TCAATACCTT CTTGAACCAT TTCCCATAAT GGTGAAAGTT CCCTCAAGAA TTTTACTCTG TCAGAAACGG CCTTAACGAC GTAGTCGATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGG ACGGATCGCT TGCCTGTAAC TTACACGCGC CTCGTATCTT TTAATGATGG AATAATTTGG GAATTTACTC TGTGTTTATT TATTTTTATG TTTTGTATTT GGATTTTAGA AAGTAAATAA AGAAGGTAGA AGAGTTACGG AATGAAGAAA AAAAAATAAA CAAAGGTTTA AAAAATTTCA ACAAAAAGCG TACTTTACAT ATATATTTAT TAGACAAGAA AAGCAGATTA AATAGATATA CATTCGATTA ACGATAAGTA AAATGTAAAA TCACAGGATT TTCGTGTGTG GTCTTCTACA CAGACAAGAT GAAACAATTC GGCATTAATA CCTGAGAGCA GGAAGAGCAA GATAAAAGGT AGTATTTGTT GGCGATCCCC CTAGAGTCTT TTACATCTTC GGAAAACAAA AACTATTTTT TCTTTAATTT CTTTTTTTAC TTTCTATTTT TAATTTATAT ATTTATATTA AAAAATTTAA ATTATAATTA TTTTTATAGC ACGTGATGAA AAGGACCCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TCTGCAGCTC TGGCCCGTGT CTCAAAATCT CTGATGTTAC ATTGCACAAG ATAAAAATAT ATCATCATGA ACAATAAAAC TGTCTGCTTA CATAAACAGT AATACAAGGG GTGTTATGAG CCATATTCAA CGGGAAACGT CGAGGCCGCG ATTAAATTCC AACATGGATG CTGATTTATA TGGGTATAAA TGGGCTCGGG CCGGGAAGCC GATCTCGGCT TGAACGAATT GTTAGGTGGC GGTACTTGGG TCGATATCAA AGTGCATCAC TTCTTCCCGT ATGCCCAACT TTGTATAGAG AGCCACTGCG GGATCGTCAC CGTAATCTGC TTGCACGTAG ATCACATAAG CACCAAGCGC GTTGGCCTCA TGCTTGAGGA GATTGATGAG CGCGGTGGCA ATGCCCTGCC TCCGGTGCTC GCCGGAGACT GCGAGATCAT AGATATAGAT CTCACTACGC GGCTGCTCAA ACTTGGGCAG AACGTAAGCC GCGAGAGCGC CAACAACCGC TTCTTGGTCG AAGGCAGCAA GCGCGATGAA TGTCTTACTA CGGAGCAAGT TCCCGAGGTA ATCGGAGTCC GGCTGATGTT GGGAGTAGGT GGCTACGTCT CCGAACTCAC GACCGAAAAG ATCAAGAGCA GCCCGCATGG ATTTGACTTG GTCAGGGCCG AGCCTACATG TGCGAATGAT GCCCATACTT GAGCCACCTA ACTTTGTTTT AGGGCGACTG CCCTGCTGCG TAACATCGTT GCTGCTGCGT AACATCGTTG CTGCTCCATA ACATCAAACA TCGACCCACG GCGTAACGCG CTTGCTGCTT GGATGCCCGA GGCATAGACT GTACAAAAAA ACAGTCATAA CAAGCCATGA AAACCGCCAC TGCGCCGTTA CCACCGCTGC GTTCGGTCAA GGTTCTGGAC CAGTTGCGTG AGCGCATACG CTACTTGCAT TACAGTTTAC GAACCGAACA GGCTTATGTC AACTGGGTTC GTGCCTTCAT CCGTTTCCAC GGTGTGCGTC ACCCGGCAAC CTTGGGCAGC AGCGAAGTCG AGGCATTTCT GTCCTGGCTG GCGAACGAGC GCAAGGTTTC GGTCTCCACG CATCGTCAGG CATTGGCGGC CTTGCTGTTC TTCTACGGCA AGGTGCTGTG CACGGATCTG CCCTGGCTTC AGGAGATCGG AAGACCTCGG CCGTCGCGGC GCTTGCCGGT GGTGCTGACC CCGGATGAAG TGGTTCGCAT CCTCGGTTTT CTGGAAGGCG AGCATCGTTT GTTCGCCCAG GACTCTAGCT ATAGTTCTAG TGGTTGGCTA CTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCTATGACC ATGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GTACCGATCC CGAGCTTGCA AATTAAAGCC TTCGAGCGTC CCAAAACCTT CTCAAGCAAG GTTTTCAGTA TAATGTTACA TGCGTACACG CGTCTGTACA GAAAAAAAAG AAAAATTTGA AATATAAATA ACGTTCTTAA TACTAACATA ACTATAAAAA AATAAATAGG GACCTAGACT TCAGGTTGTC TAACTCCTTC CTTTTCGGTT AGAGCGGATG TGGGGGGAGG GCGTGAATGT AAGCGTGACA TAACTAATTA CATGATATCG ACAAAGGAAA AGGGGCCTGT TTACTCACAG GCTTTTTTCA AGTAGGTAAT TAAGTCGTTT CTGTCTTTTT CCTTCTTCAA CCCACCAAAG GCCATCTTGG TACTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTGATAGA AATAATACAG AAGTAGATGT TGAATTAGAT TAAACTGAAG ATATATAATT TATTGGAAAA TACATAGAGT TTTTGTTGAT GCGCTTAAGC GATCAATTCA ACAACACCAC CAGCAGCTCT GATTTTTTCT TCAGCCAACT TGGAGACGAA TCTAGCTTTG ACGATAACTG GAACATTTGG AATTCTACCC TTACCCAAGA TCTTACCGTA ACCGGCTGCC AAAGTGTCAA TAACTGGAGC AGTTTCCTTA GAAGCAGATT TCAAGTATTG GTCTCTCTTG TCTTCTGGGA TCAATGTCCA CAATTTGTCC AAGTTCAAGA CTGGCTTCCA GAAATGAGCT TGTTGCTTGT GGAAGTATCT CATACCAACC TTACCGAAAT AACCTGGATG GTATTTATCC ATGTTAATTC TGTGGTGATG TTGACCACCG GCCATACCTC TACCACCGGG GTGCTTTCTG TGCTTACCGA TACGACCTTT ACCGGCTGAG ACGTGACCTC TGTGCTTTCT AGTCTTAGTG AATCTGGAAG GCATTCTTGA TTAGTTGGAT GATTGTTCTG GGATTTAATG CAAAAAAATC ACTAAGAAGG AAAAAAATCA ACGGAGAAAG CAAACGCCAT CTTAAATATA CGGGATACAG ATGAAAGGTT TGAACCTATC TGGGAAAATA CGCATTAAAC AAGCGAAAAA CTGCGAGGAA AATTGTTTGC GTCTCTGCGG GCTATTCACG CGCCAGAGGA AAAATAGGAA AAATAACAGG CATTAGAAAA ATAATTTTGA TTTTGGTAAT GTGTGGGTCC CTGGTGTACA GATGTTACAT TGGTTACAGT ACTCTTGTTT TTGCTGTGTT TTTCGATGAA TCTCCAAAAT GGTTGTTAGC ACATGGAAGA GTCACCGATG CTAAGT
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