pGWB453
$99.00
In stock
SKU
PL75313
100μg liquid plasmid(Instantly):$120
| name | pGWB453 | another name | apGWB453 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Promoter detection | Fragment type | Promoter,GateWay |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Neo/G418 | Fluorescent labeling | Red |
| Promoter | Replicon | pVS1 | |
| Competence | DB3.1 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCCAAGCTCA AGCTGCTCTA GCATTCGCCA TTCAGGCTGC GCAACTGTTG GGAAGGGCGA TCGGTGCGGG CCTCTTCGCT ATTACGCCAG CTGGCGAAAG GGGGATGTGC TGCAAGGCGA TTAAGTTGGG TAACGCCAGG GTTTTCCCAG TCACGACGTT GTAAAACGAC GGCCAGTGCC AAGCTTGCAT GCCTGCAGGT CGACTCTAGA GTTATCAACA AGTTTGTACA AAAAAGCTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCCC TCGAGGGGCC GCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATAATGT GTGGATTTTG AGTTAGGATC CGGCGAGATT TTCAGGAGCT AAGGAAGCTA AAATGGAGAA AAAAATCACT GGATATACCA CCGTTGATAT ATCCCAATGG CATCGTAAAG AACATTTTGA GGCATTTCAG TCAGTTGCTC AATGTACCTA TAACCAGACC GTTCAGCTGG ATATTACGGC CTTTTTAAAG ACCGTAAAGA AAAATAAGCA CAAGTTTTAT CCGGCCTTTA TTCACATTCT TGCCCGCCTG ATGAATGCTC ATCCGGAATT CCGTATGGCA ATGAAAGACG GTGAGCTGGT GATATGGGAT AGTGTTCACC CTTGTTACAC CGTTTTCCAT GAGCAAACTG AAACGTTTTC ATCGCTCTGG AGTGAATACC ACGACGATTT CCGGCAGTTT CTACACATAT ATTCGCAAGA TGTGGCGTGT TACGGTGAAA ACCTGGCCTA TTTCCCTAAA GGGTTTATTG AGAATATGTT TTTCGTCTCA GCCAATCCCT GGGTGAGTTT CACCAGTTTT GATTTAAACG TGGCCAATAT GGACAACTTC TTCGCCCCCG TTTTCACCAT GGGCAAATAT TATACGCAAG GCGACAAGGT GCTGATGCCG CTGGCGATTC AGGTTCATCA TGCCGTCTGT GATGGCTTCC ATGTCGGCAG AATGCTTAAT GAATTACAAC AGTACTGCGA TGAGTGGCAG GGCGGGGCGT AAAGATCTGG ATCCGGCTTA CTAAAAGCCA GATAACAGTA TGCGTATTTG CGCGCTGATT TTTGCGGTAT AAGAATATAT ACTGATATGT ATACCCGAAG TATGTCAAAA AGAGGTGTGC TATGAAGCAG CGTATTACAG TGACAGTTGA CAGCGACAGC TATCAGTTGC TCAAGGCATA TATGATGTCA ATATCTCCGG TCTGGTAAGC ACAACCATGC AGAATGAAGC CCGTCGTCTG CGTGCCGAAC GCTGGAAAGC GGAAAATCAG GAAGGGATGG CTGAGGTCGC CCGGTTTATT GAAATGAACG GCTCTTTTGC TGACGAGAAC AGGGACTGGT GAAATGCAGT TTAAGGTTTA CACCTATAAA AGAGAGAGCC GTTATCGTCT GTTTGTGGAT GTACAGAGTG ATATTATTGA CACGCCCGGG CGACGGATGG TGATCCCCCT GGCCAGTGCA CGTCTGCTGT CAGATAAAGT CTCCCGTGAA CTTTACCCGG TGGTGCATAT CGGGGATGAA AGCTGGCGCA TGATGACCAC CGATATGGCC AGTGTGCCGG TCTCCGTTAT CGGGGAAGAA GTGGCTGATC TCAGCCACCG CGAAAATGAC ATCAAAAACG CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC TTGTACAAAG TGGTTGATAA CAGCATGGCC TCCTCCGAGG ACGTCATCAA GGAGTTCATG CGCTTCAAGG TGCGCATGGA GGGCTCCGTG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGCGGC CCCCTGCCCT TCGCCTGGGA CATCCTGTCC CCTCAGTTCC AGTACGGCTC CAAGGCCTAC GTGAAGCACC CCGCCGACAT CCCCGACTAC TTGAAGCTGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT CGAGGACGGC GGCGTGGTGA CCGTGACCCA GGACTCCTCC CTGCAGGACG GCGAGTTCAT CTACAAGGTG AAGCTGCGCG GCACCAACTT CCCCTCCGAC GGCCCCGTAA TGCAGAAGAA GACCATGGGC TGGGAGGCCT CCACCGAGCG GATGTACCCC GAGGACGGCG CCCTGAAGGG CGAGATCAAG ATGAGGCTGA AGCTGAAGGA CGGCGGCCAC TACGACGCCG AGGTCAAGAC CACCTACATG GCCAAGAAGC CCGTGCAGCT GCCCGGCGCC TACAAGACCG ACATCAAGCT GGACATCACC TCCCACAACG AGGACTACAC CATCGTGGAA CAGTACGAGC GCGCCGAGGG CCGCCACTCC ACCGGCGCCT AAGCTTAGAG CTCGAATTTC CCCGATCGTT CAAACATTTG GCAATAAAGT TTCTTAAGAT TGAATCCTGT TGCCGGTCTT GCGATGATTA TCATATAATT TCTGTTGAAT TACGTTAAGC ATGTAATAAT TAACATGTAA TGCATGACGT TATTTATGAG ATGGGTTTTT ATGATTAGAG TCCCGCAATT ATACATTTAA TACGCGATAG AAAACAAAAT ATAGCGCGCA AACTAGGATA AATTATCGCG CGCGGTGTCA TCTATGTTAC TAGATCGGGA ATTGGTTCCG GAACCAATTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAGGCTGA ATTAGGCGCG CCTATTTCTG AATTCCCGAT CTAGTAACAT AGATGACACC GCGCGCGATA ATTTATCCTA GTTTGCGCGC TATATTTTGT TTTCTATCGC GTATTAAATG TATAATTGCG GGACTCTAAT CATAAAAACC CATCTCATAA ATAACGTCAT GCATTACATG TTAATTATTA CATGCTTAAC GTAATTCAAC AGAAATTATA TGATAATCAT CGCAAGACCG GCAACAGGAT TCAATCTTAA GAAACTTTAT TGCCAAATGT TTGAACGATC GGGGATCATC CGGGTCTGTG GCGGGAACTC CACGAAAATA TCCGAACGCA GCAAGATATC GCGGTGCATC TCGGTCTTGC CTGGGCAGTC GCCGCCGACG CCGTTGATGT GGACGCCGGG CCCGATCATA TTGTCGCTCA GGATCGTGGC GTTGTGCTTG TCGGCCGTTG CTGTCGTAAT GATATCGGCA CCTTCGACCG CCTGTTCCGC AGAGATCCCG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCAT CGCCGTCGGG CATGCGCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CCTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCAGAT CCGGTGCAGA TTATTTGGAT TGAGAGTGAA TATGAGACTC TAATTGGATA CCGAGGGGAA TTTATGGAAC GTCAGTGGAG CATTTTTGAC AAGAAATATT TGCTAGCTGA TAGTGACCTT AGGCGACTTT TGAACGCGCA ATAATGGTTT CTGACGTATG TGCTTAGCTC ATTAAACTCC AGAAACCCGC GGCTGAGTGG CTCCTTCAAC GTTGCGGTTC TGTCAGTTCC AAACGTAAAA CGGCTTGTCC CGCGTCATCG GCGGGGGTCA TAACGTGACT CCCTTAATTC TCCGCTCATG ATGAATTCAG AAATAAATTC AGCCTAATTC GGCGTTAATT CAGTACATTA AAAACGTCCG CAATGTGTTA TTAAGTTGTC TAAGCGTCAA TTTGTTTACA CCACAATATA TCCTGCCACC AGCCAGCCAA CAGCTCCCCG ACCGGCAGCT CGGCACAAAA TCACCACTCG ATACAGGCAG CCCATCAGTC CGGGACGGCG TCAGCGGGAG AGCCGTTGTA AGGCGGCAGA CTTTGCTCAT GTTACCGATG CTATTCGGAA GAACGGCAAC TAAGCTGCCG GGTTTGAAAC ACGGATGATC TCGCGGAGGG TAGCATGTTG ATTGTAACGA TGACAGAGCG TTGCTGCCTG TGATCAATTC GGGCACGAAC CCAGTGGACA TAAGCCTGTT CGGTTCGTAA GCTGTAATGC AAGTAGCGTA TGCGCTCACG CAACTGGTCC AGAACCTTGA CCGAACGCAG CGGTGGTAAC GGCGCAGTGG CGGTTTTCAT GGCTTGTTAT GACTGTTTTT TTGGGGTACA GTCTATGCCT CGGGCATCCA AGCAGCAAGC GCGTTACGCC GTGGGTCGAT GTTTGATGTT ATGGAGCAGC AACGATGTTA CGCAGCAGGG CAGTCGCCCT AAAACAAAGT TAAACATCAT GGGGGAAGCG GTGATCGCCG AAGTATCGAC TCAACTATCA GAGGTAGTTG GCGTCATCGA GCGCCATCTC GAACCGACGT TGCTGGCCGT ACATTTGTAC GGCTCCGCAG TGGATGGCGG CCTGAAGCCA CACAGTGATA TTGATTTGCT GGTTACGGTG ACCGTAAGGC TTGATGAAAC AACGCGGCGA GCTTTGATCA ACGACCTTTT GGAAACTTCG GCTTCCCCTG GAGAGAGCGA GATTCTCCGC GCTGTAGAAG TCACCATTGT TGTGCACGAC GACATCATTC CGTGGCGTTA TCCAGCTAAG CGCGAACTGC AATTTGGAGA ATGGCAGCGC AATGACATTC TTGCAGGTAT CTTCGAGCCA GCCACGATCG ACATTGATCT GGCTATCTTG CTGACAAAAG CAAGAGAACA TAGCGTTGCC TTGGTAGGTC CAGCGGCGGA GGAACTCTTT GATCCGGTTC CTGAACAGGA TCTATTTGAG GCGCTAAATG AAACCTTAAC GCTATGGAAC TCGCCGCCCG ACTGGGCTGG CGATGAGCGA AATGTAGTGC TTACGTTGTC CCGCATTTGG TACAGCGCAG TAACCGGCAA AATCGCGCCG AAGGATGTCG CTGCCGACTG GGCAATGGAG CGCCTGCCGG CCCAGTATCA GCCCGTCATA CTTGAAGCTA GACAGGCTTA TCTTGGACAA GAAGAAGATC GCTTGGCCTC GCGCGCAGAT CAGTTGGAAG AATTTGTCCA CTACGTGAAA GGCGAGATCA CCAAGGTAGT CGGCAAATAA TGTCTAGCTA GAAATTCGTT CAAGCCGACG CCGCTTCGCG GCGCGGCTTA ACTCAAGCGT TAGATGCACT AAGCACATAA TTGCTCACAG CCAAACTATC AGGTCAAGTC TGCTTTTATT ATTTTTAAGC GTGCATAATA AGCCCTACAC AAATTGGGAG ATATATCATG CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GGGTGCCTTG ATGTGGGCGC CGGCGGTCGA GTGGCGACGG CGCGGCTTGT CCGCGCCCTG GTAGATTGCC TGGCCGTAGG CCAGCCATTT TTGAGCGGCC AGCGGCCGCG ATAGGCCGAC GCGAAGCGGC GGGGCGTAGG GAGCGCAGCG ACCGAAGGGT AGGCGCTTTT TGCAGCTCTT CGGCTGTGCG CTGGCCAGAC AGTTATGCAC AGGCCAGGCG GGTTTTAAGA GTTTTAATAA GTTTTAAAGA GTTTTAGGCG GAAAAATCGC CTTTTTTCTC TTTTATATCA GTCACTTACA TGTGTGACCG GTTCCCAATG TACGGCTTTG GGTTCCCAAT GTACGGGTTC CGGTTCCCAA TGTACGGCTT TGGGTTCCCA ATGTACGTGC TATCCACAGG AAAGAGACCT TTTCGACCTT TTTCCCCTGC TAGGGCAATT TGCCCTAGCA TCTGCTCCGT ACATTAGGAA CCGGCGGATG CTTCGCCCTC GATCAGGTTG CGGTAGCGCA TGACTAGGAT CGGGCCAGCC TGCCCCGCCT CCTCCTTCAA ATCGTACTCC GGCAGGTCAT TTGACCCGAT CAGCTTGCGC ACGGTGAAAC AGAACTTCTT GAACTCTCCG GCGCTGCCAC TGCGTTCGTA GATCGTCTTG AACAACCATC TGGCTTCTGC CTTGCCTGCG GCGCGGCGTG CCAGGCGGTA GAGAAAACGG CCGATGCCGG GATCGATCAA AAAGTAATCG GGGTGAACCG TCAGCACGTC CGGGTTCTTG CCTTCTGTGA TCTCGCGGTA CATCCAATCA GCTAGCTCGA TCTCGATGTA CTCCGGCCGC CCGGTTTCGC TCTTTACGAT CTTGTAGCGG CTAATCAAGG CTTCACCCTC GGATACCGTC ACCAGGCGGC CGTTCTTGGC CTTCTTCGTA CGCTGCATGG CAACGTGCGT GGTGTTTAAC CGAATGCAGG TTTCTACCAG GTCGTCTTTC TGCTTTCCGC CATCGGCTCG CCGGCAGAAC TTGAGTACGT CCGCAACGTG TGGACGGAAC ACGCGGCCGG GCTTGTCTCC CTTCCCTTCC CGGTATCGGT TCATGGATTC GGTTAGATGG GAAACCGCCA TCAGTACCAG GTCGTAATCC CACACACTGG CCATGCCGGC CGGCCCTGCG GAAACCTCTA CGTGCCCGTC TGGAAGCTCG TAGCGGATCA CCTCGCCAGC TCGTCGGTCA CGCTTCGACA GACGGAAAAC GGCCACGTCC ATGATGCTGC GACTATCGCG GGTGCCCACG TCATAGAGCA TCGGAACGAA AAAATCTGGT TGCTCGTCGC CCTTGGGCGG CTTCCTAATC GACGGCGCAC CGGCTGCCGG CGGTTGCCGG GATTCTTTGC GGATTCGATC AGCGGCCGCT TGCCACGATT CACCGGGGCG TGCTTCTGCC TCGATGCGTT GCCGCTGGGC GGCCTGCGCG GCCTTCAACT TCTCCACCAG GTCATCACCC AGCGCCGCGC CGATTTGTAC CGGGCCGGAT GGTTTGCGAC CGCTCACGCC GATTCCTCGG GCTTGGGGGT TCCAGTGCCA TTGCAGGGCC GGCAGACAAC CCAGCCGCTT ACGCCTGGCC AACCGCCCGT TCCTCCACAC ATGGGGCATT CCACGGCGTC GGTGCCTGGT TGTTCTTGAT TTTCCATGCC GCCTCCTTTA GCCGCTAAAA TTCATCTACT CATTTATTCA TTTGCTCATT TACTCTGGTA GCTGCGCGAT GTATTCAGAT AGCAGCTCGG TAATGGTCTT GCCTTGGCGT ACCGCGTACA TCTTCAGCTT GGTGTGATCC TCCGCCGGCA ACTGAAAGTT GACCCGCTTC ATGGCTGGCG TGTCTGCCAG GCTGGCCAAC GTTGCAGCCT TGCTGCTGCG TGCGCTCGGA CGGCCGGCAC TTAGCGTGTT TGTGCTTTTG CTCATTTTCT CTTTACCTCA TTAACTCAAA TGAGTTTTGA TTTAATTTCA GCGGCCAGCG CCTGGACCTC GCGGGCAGCG TCGCCCTCGG GTTCTGATTC AAGAACGGTT GTGCCGGCGG CGGCAGTGCC TGGGTAGCTC ACGCGCTGCG TGATACGGGA CTCAAGAATG GGCAGCTCGT ACCCGGCCAG CGCCTCGGCA ACCTCACCGC CGATGCGCGT GCCTTTGATC GCCCGCGACA CGACAAAGGC CGCTTGTAGC CTTCCATCCG TGACCTCAAT GCGCTGCTTA ACCAGCTCCA CCAGGTCGGC GGTGGCCCAT ATGTCGTAAG GGCTTGGCTG CACCGGAATC AGCACGAAGT CGGCTGCCTT GATCGCGGAC ACAGCCAAGT CCGCCGCCTG GGGCGCTCCG TCGATCACTA CGAAGTCGCG CCGGCCGATG GCCTTCACGT CGCGGTCAAT CGTCGGGCGG TCGATGCCGA CAACGGTTAG CGGTTGATCT TCCCGCACGG CCGCCCAATC GCGGGCACTG CCCTGGGGAT CGGAATCGAC TAACAGAACA TCGGCCCCGG CGAGTTGCAG GGCGCGGGCT AGATGGGTTG CGATGGTCGT CTTGCCTGAC CCGCCTTTCT GGTTAAGTAC AGCGATAACC TTCATGCGTT CCCCTTGCGT ATTTGTTTAT TTACTCATCG CATCATATAC GCAGCGACCG CATGACGCAA GCTGTTTTAC TCAAATACAC ATCACCTTTT TAGACGGCGG CGCTCGGTTT CTTCAGCGGC CAAGCTGGCC GGCCAGGCCG CCAGCTTGGC ATCAGACAAA CCGGCCAGGA TTTCATGCAG CCGCACGGTT GAGACGTGCG CGGGCGGCTC GAACACGTAC CCGGCCGCGA TCATCTCCGC CTCGATCTCT TCGGTAATGA AAAACGGTTC GTCCTGGCCG TCCTGGTGCG GTTTCATGCT TGTTCCTCTT GGCGTTCATT CTCGGCGGCC GCCAGGGCGT CGGCCTCGGT CAATGCGTCC TCACGGAAGG CACCGCGCCG CCTGGCCTCG GTGGGCGTCA CTTCCTCGCT GCGCTCAAGT GCGCGGTACA GGGTCGAGCG ATGCACGCCA AGCAGTGCAG CCGCCTCTTT CACGGTGCGG CCTTCCTGGT CGATCAGCTC GCGGGCGTGC GCGATCTGTG CCGGGGTGAG GGTAGGGCGG GGGCCAAACT TCACGCCTCG GGCCTTGGCG GCCTCGCGCC CGCTCCGGGT GCGGTCGATG ATTAGGGAAC GCTCGAACTC GGCAATGCCG GCGAACACGG TCAACACCAT GCGGCCGGCC GGCGTGGTGG TGTCGGCCCA CGGCTCTGCC AGGCTACGCA GGCCCGCGCC GGCCTCCTGG ATGCGCTCGG CAATGTCCAG TAGGTCGCGG GTGCTGCGGG CCAGGCGGTC TAGCCTGGTC ACTGTCACAA CGTCGCCAGG GCGTAGGTGG TCAAGCATCC TGGCCAGCTC CGGGCGGTCG CGCCTGGTGC CGGTGATCTT CTCGGAAAAC AGCTTGGTGC AGCCGGCCGC GTGCAGTTCG GCCCGTTGGT TGGTCAAGTC CTGGTCGTCG GTGCTGACGC GGGCATAGCC CAGCAGGCCA GCGGCGGCGC TCTTGTTCAT GGCGTAATGT CTCCGGTTCT AGTCGCAAGT ATTCTACTTT ATGCGACTAA AACACGCGAC AAGAAAACGC CAGGAAAAGG GCAGGGCGGC AGCCTGTCGC GTAACTTAGG ACTTGTGCGA CATGTCGTTT TCAGAAGACG GCTGCACTGA ACGTCAGAAG CCGACTGCAC TATAGCAGCG GAGGGGTTGG ATCAAAGTAC TTTGATCCCG AGGGGAACCC TGTGGTTGGC ATGCACATAC AAATGGACGA ACGGATAAAC CT
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