pCDNA3.1-USP32-3×FLAG
$128.00
In stock
SKU
PL75269
NM_032582.4BC156264.1;NY-REN-60;USP10
100μg liquid plasmid(Instantly):$149
| name | pCDNA3.1-USP32-3×FLAG | another name | apCDNA3.1-USP32-3×FLAG |
| Insert Size (bp) | 4812bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT TAATCTTGGT ACCGAGCTCG GATCCGCCAC CATGGGTGCC AAGGAGTCAC GGATCGGATT CCTCAGCTAC GAGGAGGCGC TGAGGAGAGT TACAGATGTA GAGCTAAAAC GACTGAAGGA TGCTTTCAAG AGGACCTGTG GACTCTCATA TTACATGGGC CAGCACTGCT TCATCCGGGA AGTGCTTGGG GATGGAGTGC CTCCAAAGGT TGCTGAGGTG ATTTACTGTT CTTTTGGTGG AACATCCAAA GGGCTGCACT TCAATAATTT AATAGTTGGA CTTGTCCTCC TTACAAGAGG CAAAGATGAA GAGAAAGCAA AATACATTTT TAGTCTTTTT TCAAGTGAAT CTGGGAACTA TGTTATACGG GAAGAAATGG AAAGAATGCT CCACGTGGTG GATGGTAAAG TCCCAGATAC ACTCAGGAAG TGTTTCTCAG AGGGTGAAAA GGTAAACTAT GAAAAGTTTA GAAATTGGCT TTTTCTAAAC AAAGATGCTT TTACTTTCTC TCGATGGCTT CTATCTGGAG GTGTGTATGT TACCCTCACT GATGATAGTG ATACTCCTAC TTTCTACCAA ACTCTGGCTG GAGTCACACA TTTGGAGGAA TCAGACATCA TTGATCTTGA GAAACGCTAT TGGTTATTGA AGGCTCAATC CCGGACTGGA CGATTTGATT TAGAGACATT TGGCCCATTG GTTTCACCAC CTATTCGTCC ATCTCTAAGT GAAGGTTTGT TTAATGCTTT TGATGAAAAT CGTGACAATC ACATAGATTT TAAGGAGATA TCCTGTGGGT TATCAGCCTG TTGCAGGGGA CCCCTGGCTG AAAGACAAAA ATTTTGCTTC AAGGTATTTG ATGTTGACCG TGATGGAGTT CTCTCCAGGG TTGAACTGAG AGACATGGTG GTTGCACTTT TAGAAGTCTG GAAGGACAAC CGCACTGATG ATATTCCTGA ATTACATATG GATCTCTCTG ATATTGTAGA AGGCATACTG AATGCACATG ACACCACAAA GATGGGTCAT CTTACTCTGG AAGACTATCA GATCTGGAGT GTGAAAAATG TTCTTGCCAA TGAGTTTTTG AACCTCCTTT TCCAGGTGTG TCACATAGTT CTGGGGTTAA GACCAGCTAC TCCGGAAGAA GAAGGACAAA TTATTAGAGG ATGGTTAGAA CGAGAGAGCA GGTATGGTCT GCAAGCAGGA CACAACTGGT TTATCATCTC CATGCAGTGG TGGCAACAGT GGAAAGAATA TGTCAAATAC GATGCCAACC CTGTGGTAAT TGAGCCATCA TCTGTTTTGA ATGGAGGAAA ATACTCATTT GGAACTGCAG CCCATCCTAT GGAGCAGGTC GAAGATAGAA TTGGAAGCAG CCTCAGTTAC GTGAATACTA CAGAAGAGAA ATTTTCAGAC AACATTTCTA CTGCATCTGA AGCCTCAGAA ACTGCTGGCA GCGGCTTTCT GTATTCTGCC ACACCAGGGG CAGATGTTTG CTTTGCTCGA CAACATAACA CTTCTGACAA TAACAACCAG TGTTTGCTGG GAGCCAATGG GAATATTTTG TTGCACCTTA ACCCTCAGAA ACCAGGGGCT ATTGATAATC AGCCATTAGT AACTCAAGAA CCAGTAAAGG CTACATCATT AACACTAGAA GGAGGACGAT TAAAACGAAC TCCACAGCTG ATTCATGGAA GAGACTATGA AATGGTCCCA GAACCTGTGT GGAGAGCACT TTATCACTGG TATGGAGCAA ACCTGGCCTT ACCTAGACCA GTTATCAAGA ACAGCAAGAC AGACATCCCA GAGCTGGAAT TATTTCCCCG CTATCTTCTC TTCCTGAGAC AGCAGCCTGC CACTCGGACA CAGCAGTCTA ACATCTGGGT GAATATGGGA AATGTACCTT CTCCGAATGC ACCTTTAAAG CGGGTATTAG CCTATACAGG CTGTTTTAGT CGAATGCAGA CCATCAAGGA AATTCACGAA TATCTATCTC AAAGGCTGCG CATTAAAGAG GAAGATATGC GCCTGTGGCT ATACAACAGT GAGAACTACC TTACTCTTCT GGATGATGAG GATCATAAAT TGGAATATTT GAAAATCCAG GATGAACAAC ACCTGGTAAT TGAAGTTCGC AACAAAGATA TGAGTTGGCC TGAGGAGATG TCTTTTATAG CAAATAGTAG TAAAATAGAT AGACACAAGG TTCCCACAGA AAAGGGAGCC ACAGGTCTAA GCAATCTGGG AAACACATGC TTCATGAACT CAAGCATCCA GTGTGTTAGT AACACACAGC CACTGACACA GTATTTTATC TCAGGGAGAC ATCTTTATGA ACTCAACAGG ACAAATCCCA TTGGTATGAA GGGGCATATG GCTAAATGCT ATGGTGATTT AGTGCAGGAA CTTTGGAGTG GAACTCAGAA GAATGTTGCC CCATTAAAGC TTCGGTGGAC CATAGCAAAA TATGCTCCCA GGTTTAATGG GTTTCAGCAA CAGGACTCCC AAGAACTTCT GGCTTTTCTC TTGGATGGTC TTCATGAAGA TCTTAATCGA GTCCATGAAA AGCCATATGT GGAACTGAAG GACAGTGATG GGCGACCAGA CTGGGAAGTA GCTGCAGAGG CCTGGGACAA CCATCTAAGA AGAAATAGAT CAATTGTTGT GGATTTGTTC CATGGGCAGC TAAGATCTCA AGTAAAATGC AAGACATGTG GGCATATAAG TGTCCGATTT GACCCTTTCA ATTTTTTGTC TTTGCCACTA CCAATGGACA GTTATATGCA CTTAGAAATA ACAGTGATTA AGTTAGATGG TACTACCCCT GTACGGTATG GACTAAGACT GAATATGGAT GAAAAGTACA CAGGTTTAAA AAAACAGCTG AGTGATCTCT GTGGACTTAA TTCAGAACAA ATCCTTCTAG CAGAAGTACA TGGTTCCAAC ATAAAGAACT TTCCTCAGGA CAACCAAAAA GTACGACTCT CAGTGAGTGG ATTTTTGTGT GCATTTGAAA TTCCTGTCCC TGTGTCTCCA ATTTCAGCTT CTAGTCCAAC ACAGACAGAT TTCTCCTCTT CGCCATCTAC AAATGAAATG TTCACCCTAA CTACCAATGG GGACCTACCC CGACCAATAT TCATCCCCAA TGGAATGCCA AACACTGTTG TGCCATGTGG AACTGAGAAG AACTTCACAA ATGGAATGGT TAATGGTCAC ATGCCATCTC TTCCTGACAG CCCCTTTACA GGTTACATCA TTGCAGTCCA CCGAAAAATG ATGAGGACAG AACTGTATTT CCTGTCATCT CAGAAGAATC GCCCCAGCCT CTTTGGAATG CCATTGATTG TTCCATGTAC TGTGCATACC CGGAAGAAAG ACCTATATGA TGCGGTTTGG ATTCAAGTAT CCCGGTTAGC GAGCCCACTC CCACCTCAGG AAGCTAGTAA TCATGCCCAG GATTGTGACG ACAGTATGGG CTATCAATAT CCATTCACTC TACGAGTTGT GCAGAAAGAT GGGAACTCCT GTGCTTGGTG CCCATGGTAT AGATTTTGCA GAGGCTGTAA AATTGATTGT GGGGAAGACA GAGCTTTCAT TGGAAATGCC TATATCGCTG TGGATTGGGA TCCCACAGCC CTTCACCTTC GCTATCAAAC ATCCCAGGAA AGGGTTGTAG ATGAGCATGA GAGTGTGGAG CAGAGTCGGC GAGCGCAAGC CGAGCCCATC AACCTGGACA GCTGTCTCCG TGCTTTCACC AGTGAGGAAG AGCTAGGGGA AAATGAGATG TACTACTGTT CCAAGTGTAA GACCCACTGC TTAGCAACAA AGAAGCTGGA TCTCTGGAGG CTTCCACCCA TCCTGATTAT TCACCTTAAG CGATTTCAAT TTGTAAATGG TCGGTGGATA AAATCACAGA AAATTGTCAA ATTTCCTCGG GAAAGTTTTG ATCCAAGTGC TTTTTTGGTA CCAAGAGACC CGGCTCTCTG CCAGCATAAA CCACTCACAC CCCAGGGGGA TGAGCTCTCT GAGCCCAGGA TTCTGGCAAG GGAGGTGAAG AAAGTGGATG CGCAGAGTTC GGCTGGGGAA GAGGACGTGC TCCTGAGCAA AAGCCCATCC TCACTCAGCG CTAACATCAT CAGCAGCCCG AAAGGTTCTC CTTCTTCATC AAGAAAAAGT GGAACCAGCT GTCCCTCCAG CAAAAACAGC AGCCCTAATA GCAGCCCACG GACTTTGGGG AGGAGCAAAG GGAGGCTCCG GCTGCCCCAG ATTGGCAGCA AAAATAAACT GTCAAGTAGT AAAGAGAACT TGGATGCCAG CAAAGAAAAT GGGGCTGGGC AGATATGTGA GCTGGCTGAC GCCTTGAGTC GAGGGCATGT GCTGGGGGGC AGCCAACCAG AGTTGGTCAC TCCTCAGGAC CATGAGGTAG CTTTGGCCAA TGGATTCCTT TATGAGCATG AAGCATGTGG CAATGGCTAC AGCAATGGTC AGCTTGGAAA CCACAGTGAA GAAGACAGCA CTGATGACCA AAGAGAAGAT ACTCGTATTA AGCCTATTTA TAATCTATAT GCAATTTCGT GCCATTCAGG AATTCTGGGT GGGGGCCATT ACGTCACTTA TGCCAAAAAC CCAAACTGCA AGTGGTACTG TTACAATGAC AGCAGCTGTA AGGAACTTCA CCCGGATGAA ATTGACACCG ACTCTGCCTA CATTCTTTTC TATGAGCAGC AGGGGATAGA CTATGCACAA TTTCTGCCAA AGACTGATGG CAAAAAGATG GCAGACACAA GCAGTATGGA TGAAGACTTT GAGTCTGATT ACAAAAAGTA CTGTGTGTTA CAGCTCGAGT CTAGAGGGCC CTTCGACTAC AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TGAGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC
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