pCMV-ITGA2B-T2A-ITGB3
$142.00
In stock
SKU
PL75260
NM_000419.4;NM_000212.2
100μg liquid plasmid(Instantly):$163
| name | pCMV-ITGA2B-T2A-ITGB3 | another name | apCMV-ITGA2B-T2A-ITGB3 |
| Insert Size (bp) | 5484bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CGATATCGCC ACCATGGCCA GAGCTTTGTG TCCACTGCAA GCCCTCTGGC TTCTGGAGTG GGTGCTGCTG CTCTTGGGAC CTTGTGCTGC CCCTCCAGCC TGGGCCTTGA ACCTGGACCC AGTGCAGCTC ACCTTCTATG CAGGCCCCAA TGGCAGCCAG TTTGGATTTT CACTGGACTT CCACAAGGAC AGCCATGGGA GAGTGGCCAT CGTGGTGGGC GCCCCGCGGA CCCTGGGCCC CAGCCAGGAG GAGACGGGCG GCGTGTTCCT GTGCCCCTGG AGGGCCGAGG GCGGCCAGTG CCCCTCGCTG CTCTTTGACC TCCGTGATGA GACCCGAAAT GTAGGCTCCC AAACTTTACA AACCTTCAAG GCCCGCCAAG GACTGGGGGC GTCGGTCGTC AGCTGGAGCG ACGTCATTGT GGCCTGCGCC CCCTGGCAGC ACTGGAACGT CCTAGAAAAG ACTGAGGAGG CTGAGAAGAC GCCCGTAGGT AGCTGCTTTT TGGCTCAGCC AGAGAGCGGC CGCCGCGCCG AGTACTCCCC CTGTCGCGGG AACACCCTGA GCCGCATTTA CGTGGAAAAT GATTTTAGCT GGGACAAGCG TTACTGTGAA GCGGGCTTCA GCTCCGTGGT CACTCAGGCC GGAGAGCTGG TGCTTGGGGC TCCTGGCGGC TATTATTTCT TAGGTCTCCT GGCCCAGGCT CCAGTTGCGG ATATTTTCTC GAGTTACCGC CCAGGCATCC TTTTGTGGCA CGTGTCCTCC CAGAGCCTCT CCTTTGACTC CAGCAACCCA GAGTACTTCG ACGGCTACTG GGGGTACTCG GTGGCCGTGG GCGAGTTCGA CGGGGATCTC AACACTACAG AATATGTCGT CGGTGCCCCC ACTTGGAGCT GGACCCTGGG AGCGGTGGAA ATTTTGGATT CCTACTACCA GAGGCTGCAT CGGCTGCGCG GAGAGCAGAT GGCGTCGTAT TTTGGGCATT CAGTGGCTGT CACTGACGTC AACGGGGATG GGAGGCATGA TCTGCTGGTG GGCGCTCCAC TGTATATGGA GAGCCGGGCA GACCGAAAAC TGGCCGAAGT GGGGCGTGTG TATTTGTTCC TGCAGCCGCG AGGCCCCCAC GCGCTGGGTG CCCCCAGCCT CCTGCTGACT GGCACACAGC TCTATGGGCG ATTCGGCTCT GCCATCGCAC CCCTGGGCGA CCTCGACCGG GATGGCTACA ATGACATTGC AGTGGCTGCC CCCTACGGGG GTCCCAGTGG CCGGGGCCAA GTGCTGGTGT TCCTGGGTCA GAGTGAGGGG CTGAGGTCAC GTCCCTCCCA GGTCCTGGAC AGCCCCTTCC CCACAGGCTC TGCCTTTGGC TTCTCCCTTC GAGGTGCCGT AGACATCGAT GACAACGGAT ACCCAGACCT GATCGTGGGA GCTTACGGGG CCAACCAGGT GGCTGTGTAC AGAGCTCAGC CAGTGGTGAA GGCCTCTGTC CAGCTACTGG TGCAAGATTC ACTGAATCCT GCTGTGAAGA GCTGTGTCCT ACCTCAGACC AAGACACCCG TGAGCTGCTT CAACATCCAG ATGTGTGTTG GAGCCACTGG GCACAACATT CCTCAGAAGC TATCCCTAAA TGCCGAGCTG CAGCTGGACC GGCAGAAGCC CCGCCAGGGC CGGCGGGTGC TGCTGCTGGG CTCTCAACAG GCAGGCACCA CCCTGAACCT GGATCTGGGC GGAAAGCACA GCCCCATCTG CCACACCACC ATGGCCTTCC TTCGAGATGA GGCAGACTTC CGGGACAAGC TGAGCCCCAT TGTGCTCAGC CTCAATGTGT CCCTACCGCC CACGGAGGCT GGAATGGCCC CTGCTGTCGT GCTGCATGGA GACACCCATG TGCAGGAGCA GACACGAATC GTCCTGGACT GTGGGGAAGA TGACGTATGT GTGCCCCAGC TTCAGCTCAC TGCCAGCGTG ACGGGCTCCC CGCTCCTAGT TGGGGCAGAT AATGTCCTGG AGCTGCAGAT GGACGCAGCC AACGAGGGCG AGGGGGCCTA TGAAGCAGAG CTGGCCGTGC ACCTGCCCCA GGGCGCCCAC TACATGCGGG CCCTAAGCAA TGTCGAGGGC TTTGAGAGAC TCATCTGTAA TCAGAAGAAG GAGAATGAGA CCAGGGTGGT GCTGTGTGAG CTGGGCAACC CCATGAAGAA GAACGCCCAG ATAGGAATCG CGATGTTGGT GAGCGTGGGG AATCTGGAAG AGGCTGGGGA GTCTGTGTCC TTCCAGCTGC AGATACGGAG CAAGAACAGC CAGAATCCAA ACAGCAAGAT TGTGCTGCTG GACGTGCCGG TCCGGGCAGA GGCCCAAGTG GAGCTGCGAG GGAACTCCTT TCCAGCCTCC CTGGTGGTGG CAGCAGAAGA AGGTGAGAGG GAGCAGAACA GCTTGGACAG CTGGGGACCC AAAGTGGAGC ACACCTATGA GCTCCACAAC AATGGCCCTG GGACTGTGAA TGGTCTTCAC CTCAGCATCC ACCTTCCGGG ACAGTCCCAG CCCTCCGACC TGCTCTACAT CCTGGATATA CAGCCCCAGG GGGGCCTTCA GTGCTTCCCA CAGCCTCCTG TCAACCCTCT CAAGGTGGAC TGGGGGCTGC CCATCCCCAG CCCCTCCCCC ATTCACCCGG CCCATCACAA GCGGGATCGC AGACAGATCT TCCTGCCAGA GCCCGAGCAG CCCTCGAGGC TTCAGGATCC AGTTCTCGTA AGCTGCGACT CGGCGCCCTG TACTGTGGTG CAGTGTGACC TGCAGGAGAT GGCGCGCGGG CAGCGGGCCA TGGTCACGGT GCTGGCCTTC CTGTGGCTGC CCAGCCTCTA CCAGAGGCCT CTGGATCAGT TTGTGCTGCA GTCGCACGCA TGGTTCAACG TGTCCTCCCT CCCCTATGCG GTGCCCCCGC TCAGCCTGCC CCGAGGGGAA GCTCAGGTGT GGACACAGCT GCTCCGGGCC TTGGAGGAGA GGGCCATTCC AATCTGGTGG GTGCTGGTGG GTGTGCTGGG TGGCCTGCTG CTGCTCACCA TCCTGGTCCT GGCCATGTGG AAGGTCGGCT TCTTCAAGCG GAACCGGCCA CCCCTGGAAG AAGATGATGA AGAGGGGGAG GAGGGCAGAG GAAGTCTTCT AACATGCGGT GACGTGGAGG AGAATCCCGG CCCTGCTAGC GCCACCATGC GAGCGCGGCC GCGGCCCCGG CCGCTCTGGG CGACTGTGCT GGCGCTGGGG GCGCTGGCGG GCGTTGGCGT AGGAGGGCCC AACATCTGTA CCACGCGAGG TGTGAGCTCC TGCCAGCAGT GCCTGGCTGT GAGCCCCATG TGTGCCTGGT GCTCTGATGA GGCCCTGCCT CTGGGCTCAC CTCGCTGTGA CCTGAAGGAG AATCTGCTGA AGGATAACTG TGCCCCAGAA TCCATCGAGT TCCCAGTGAG TGAGGCCCGA GTACTAGAGG ACAGGCCCCT CAGCGACAAG GGCTCTGGAG ACAGCTCCCA GGTCACTCAA GTCAGTCCCC AGAGGATTGC ACTCCGGCTC CGGCCAGATG ATTCGAAGAA TTTCTCCATC CAAGTGCGGC AGGTGGAGGA TTACCCTGTG GACATCTACT ACTTGATGGA CCTGTCTTAC TCCATGAAGG ATGATCTGTG GAGCATCCAG AACCTGGGTA CCAAGCTGGC CACCCAGATG CGAAAGCTCA CCAGTAACCT GCGGATTGGC TTCGGGGCAT TTGTGGACAA GCCTGTGTCA CCATACATGT ATATCTCCCC ACCAGAGGCC CTCGAAAACC CCTGCTATGA TATGAAGACC ACCTGCTTGC CCATGTTTGG CTACAAACAC GTGCTGACGC TAACTGACCA GGTGACCCGC TTCAATGAGG AAGTGAAGAA GCAGAGTGTG TCACGGAACC GAGATGCCCC AGAGGGTGGC TTTGATGCCA TCATGCAGGC TACAGTCTGT GATGAAAAGA TTGGCTGGAG GAATGATGCA TCCCACTTGC TGGTGTTTAC CACTGATGCC AAGACTCATA TAGCATTGGA CGGAAGGCTG GCAGGCATTG TCCAGCCTAA TGACGGGCAG TGTCATGTTG GTAGTGACAA TCATTACTCT GCCTCCACTA CCATGGATTA TCCCTCTTTG GGGCTGATGA CTGAGAAGCT ATCCCAGAAA AACATCAATT TGATCTTTGC AGTGACTGAA AATGTAGTCA ATCTCTATCA GAACTATAGT GAGCTCATCC CAGGGACCAC AGTTGGGGTT CTGTCCATGG ATTCCAGCAA TGTCCTCCAG CTCATTGTTG ATGCTTATGG GAAAATCCGT TCTAAAGTAG AGCTGGAAGT GCGTGACCTC CCTGAAGAGT TGTCTCTATC CTTCAATGCC ACCTGCCTCA ACAATGAGGT CATCCCTGGC CTCAAGTCTT GTATGGGACT CAAGATTGGA GACACGGTGA GCTTCAGCAT TGAGGCCAAG GTGCGAGGCT GTCCCCAGGA GAAGGAGAAG TCCTTTACCA TAAAGCCCGT GGGCTTCAAG GACAGCCTGA TCGTCCAGGT CACCTTTGAT TGTGACTGTG CCTGCCAGGC CCAAGCTGAA CCTAATAGCC ATCGCTGCAA CAATGGCAAT GGGACCTTTG AGTGTGGGGT ATGCCGTTGT GGGCCTGGCT GGCTGGGATC CCAGTGTGAG TGCTCAGAGG AGGACTATCG CCCTTCCCAG CAGGACGAAT GCAGCCCCCG GGAGGGTCAG CCCGTCTGCA GCCAGCGGGG CGAGTGCCTC TGTGGTCAAT GTGTCTGCCA CAGCAGTGAC TTTGGCAAGA TCACGGGCAA GTACTGCGAG TGTGACGACT TCTCCTGTGT CCGCTACAAG GGGGAGATGT GCTCAGGCCA TGGCCAGTGC AGCTGTGGGG ACTGCCTGTG TGACTCCGAC TGGACCGGCT ACTACTGCAA CTGTACCACG CGTACTGACA CCTGCATGTC CAGCAATGGG CTGCTGTGCA GCGGCCGCGG CAAGTGTGAA TGTGGCAGCT GTGTCTGTAT CCAGCCGGGC TCCTATGGGG ACACCTGTGA GAAGTGCCCC ACCTGCCCAG ATGCCTGCAC CTTTAAGAAA GAATGTGTGG AGTGTAAGAA GTTTGACCGG GGAGCCCTAC ATGACGAAAA TACCTGCAAC CGTTACTGCC GTGACGAGAT TGAGTCAGTG AAAGAGCTTA AGGACACTGG CAAGGATGCA GTGAATTGTA CCTATAAGAA TGAGGATGAC TGTGTCGTCA GATTCCAGTA CTATGAAGAT TCTAGTGGAA AGTCCATCCT GTATGTGGTA GAAGAGCCAG AGTGTCCCAA GGGCCCTGAC ATCCTGGTGG TCCTGCTCTC AGTGATGGGG GCCATTCTGC TCATTGGCCT TGCCGCCCTG CTCATCTGGA AACTCCTCAT CACCATCCAC GACCGAAAAG AATTCGCTAA ATTTGAGGAA GAACGCGCCA GAGCAAAATG GGACACAGCC AACAACCCAC TGTATAAAGA GGCCACGTCT ACCTTCACCA ATATCACGTA CCGGGGCACT TAAACCGGTC GTACGTCTCC GGATTCGAAT TCGGATCCGC GGCCGCATAG ATAACTGATC CAGTGTGCTG GAATTAATTC GCTGTCTGCG AGGGCCAGCT GTTGGGGTGA GTACTCCCTC TCAAAAGCGG GCATGACTTC TGCGCTAAGA TTGTCAGTTT CCAAAAACGA GGAGGATTTG ATATTCACCT GGCCCGCGGT GATGCCTTTG AGGGTGGCCG CGTCCATCTG GTCAGAAAAG ACAATCTTTT TGTTGTCAAG CTTGAGGTGT GGCAGGCTTG AGATCTGGCC ATACACTTGA GTGACAATGA CATCCACTTT GCCTTTCTCT CCACAGGTGT CCACTCCCAG GTCCAACTGC AGGTCGAGCA TGCATCTAGG GCGGCCAATT CCGCCCCTCT CCCCCCCCCC CCTCTCCCTC CCCCCCCCCT AACGTTACTG GCCGAAGCCG CTTGGAATAA GGCCGGTGTG CGTTTGTCTA TATGTTATTT TCCACCATAT TGCCGTCTTT TGGCAATGTG AGGGCCCGGA AACCTGGCCC TGTCTTCTTG ACGAGCATTC CTAGGGGTCT TTCCCCTCTC GCCAAAGGAA TGCAAGGTCT GTTGAATGTC GTGAAGGAAG CAGTTCCTCT GGAAGCTTCT TGAAGACAAA CAACGTCTGT AGCGACCCTT TGCAGGCAGC GGAACCCCCC ACCTGGCGAC AGGTGCCTCT GCGGCCAAAA GCCACGTGTA TAAGATACAC CTGCAAAGGC GGCACAACCC CAGTGCCACG TTGTGAGTTG GATAGTTGTG GAAAGAGTCA AATGGCTCTC CTCAAGCGTA TTCAACAAGG GGCTGAAGGA TGCCCAGAAG GTACCCCATT GTATGGGATC TGATCTGGGG CCTCGGTGCA CATGCTTTAC ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA ACACGATGAT AAGCTTGCCA CAACCCACAA GGAGACGACC TTCCATGACC GAGTACAAGC CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGACGCCCG CCCCACGACC CGCAGCGCCC GACCGAAAGG AGCGCACGAC CCCATGGCTC CGACCGAAGC CACCCGGGGC GGCCCCGCCG ACCCCGCACC CGCCCCCGAG GCCCACCGAC TCTAGATAAC TGATCATAAT CAGCCATACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAA CGCTCGAGTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTC
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