pCDNA3.1-Ogt-m-myc
$113.00
In stock
SKU
PL75258
NM_139144.4;1110038P24Rik;4831420N21Rik
100μg liquid plasmid(Instantly):$134
| name | pCDNA3.1-Ogt-m-myc | another name | apCDNA3.1-Ogt-m-myc |
| Insert Size (bp) | 3138bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG CCACATGGCG TCTTCCGTGG GCAACGTGGC CGACAGTACA GAACCAACGA AACGTATGCT TTCCTTCCAA GGGTTAGCTG AGTTGGCACA TCGAGAATAT CAGGCAGGAG ATTTTGAGGC AGCTGAGAGA CACTGCATGC AGCTCTGGAG ACAAGAGCCT GACAATACTG GTGTTCTTTT ATTACTTTCA TCTATACACT TCCAGTGTCG AAGGCTGGAC AGATCTGCTC ATTTTAGCAC CTTGGCAATT AAACAGAATC CCCTTCTAGC AGAAGCCTAT TCGAATTTGG GAAATGTGTA CAAGGAAAGA GGGCAGTTGC AGGAAGCAAT CGAGCATTAT CGACATGCCT TGCGGCTGAA GCCTGATTTC ATTGATGGTT ATATTAACCT GGCAGCAGCC TTGGTAGCAG CAGGTGACAT GGAAGGAGCA GTACAAGCCT ATGTCTCTGC TCTTCAGTAC AATCCTGATT TGTACTGTGT TCGCAGTGAC CTGGGGAACC TGCTCAAAGC CCTGGGTCGC TTGGAAGAAG CCAAGGCATG TTATTTGAAA GCAATTGAGA CGCAACCAAA CTTTGCAGTA GCCTGGAGTA ATCTCGGCTG TGTTTTCAAT GCACAAGGGG AGATTTGGCT GGCTATTCAT CACTTTGAAA AGGCTGTCAC CCTTGACCCA AATTTTCTGG ATGCTTATAT CAATTTAGGA AATGTCTTGA AAGAGGCACG CATTTTTGAC AGAGCTGTCG CAGCTTATCT TCGTGCCTTA AGTTTGAGCC CAAATCATGC GGTGGTGCAC GGCAACCTGG CTTGTGTGTA CTACGAGCAA GGCCTAATAG ACCTGGCCAT TGATACCTAC AGGAGAGCTA TCGAACTGCA ACCCCATTTC CCCGATGCTT ACTGCAACCT AGCAAATGCT CTCAAAGAGA AGGGCAGTGT TGCTGAAGCA GAAGATTGTT ATAACACAGC TCTTCGTCTG TGTCCTACTC ATGCAGACTC TTTGAATAAC CTTGCCAACA TCAAACGGGA ACAGGGCAAC ATTGAAGAGG CAGTTCGCCT GTATCGCAAA GCATTAGAAG TCTTCCCAGA GTTTGCTGCT GCACATTCCA ATTTAGCAAG TGTACTGCAA CAGCAGGGCA AGCTGCAGGA AGCACTGATG CACTATAAAG AAGCCATACG AATTAGTCCT ACATTTGCTG ATGCTTATTC CAATATGGGA AACACTCTAA AGGAGATGCA GGATGTGCAG GGCGCTTTGC AGTGTTATAC TCGTGCCATC CAGATTAATC CTGCCTTTGC TGATGCACAC AGCAATCTGG CCTCCATTCA CAAGGATTCA GGGAATATCC CAGAAGCAAT AGCTTCTTAC CGCACAGCTC TGAAACTTAA GCCTGACTTT CCTGATGCTT ATTGTAACTT GGCTCATTGC CTACAGATTG TCTGTGATTG GACAGACTAT GATGAGCGGA TGAAGAAATT GGTTAGTATT GTAGCTGAGC AGCTAGAGAA GAATAGACTG CCTTCTGTCC ATCCTCACCA TAGCATGCTG TACCCTCTTT CCCATGGCTT CAGGAAGGCT ATTGCAGAGA GGCATGGGAA TCTCTGCTTG GATAAGATTA ATGTCCTTCA TAAACCACCA TATGAACATC CAAAAGACTT GAAGCTCAGT GATGGCCGAT TGCGTGTAGG CTATGTGAGT TCTGACTTCG GGAATCACCC TACTTCACAC CTTATGCAGT CTATTCCAGG CATGCATAAT CCTGATAAGT TTGAGGTATT CTGCTATGCC TTGAGCCCGG ATGATGGTAC AAACTTTCGA GTGAAGGTGA TGGCGGAAGC CAATCATTTC ATTGATCTTT CTCAGATTCC TTGTAATGGA AAAGCAGCCG ACCGCATCCA CCAAGATGGA ATTCACATCC TTGTGAATAT GAATGGGTAT ACCAAGGGTG CTCGGAATGA GCTCTTTGCT CTTAGGCCAG CTCCTATTCA GGCCATGTGG CTGGGCTACC CTGGGACTAG TGGTGCACTG TTCATGGATT ACATCATCAC TGATCAGGAA ACTTCCCCAG CTGAAGTTGC AGAGCAGTAT TCTGAGAAAC TGGCTTATAT GCCCCATACT TTCTTTATTG GTGATCATGC TAATATGTTC CCTCACCTGA AGAAAAAAGC AGTCATCGAT TTTAAATCCA ATGGGCACAT TTATGATAAT CGGATAGTTC TGAATGGCAT CGATCTCAAA GCATTTCTCG ATAGCCTACC CGATGTGAAG ATTGTCAAGA TGAAATGTCC TGATGGAGGT GACAATCCAG ACAGCAGTAA CACAGCTCTT AATATGCCCG TTATTCCCAT GAATACGATT GCAGAAGCAG TAATTGAAAT GATTAACAGA GGGCAGATTC AGATAACAAT TAACGGATTC AGTATTAGCA ATGGACTGGC GACTACACAG ATTAATAATA AGGCTGCAAC CGGAGAGGAA GTTCCCCGTA CCATTATTGT AACCACCCGT TCCCAGTATG GGCTACCAGA AGATGCCATT GTGTACTGTA ACTTTAATCA GTTATATAAA ATTGACCCAT CTACCCTGCA GATGTGGGCA AATATTCTGA AACGTGTGCC TAACAGCGTG CTTTGGCTGT TGCGTTTTCC AGCAGTAGGA GAACCCAATA TTCAACAATA TGCACAAAAT ATGGGCCTTC CCCAGAACCG TATCATTTTC TCACCTGTGG CTCCTAAAGA GGAGCATGTC AGGAGAGGTC AGCTGGCTGA TGTCTGCCTG GATACTCCTT TGTGTAATGG ACACACCACA GGGATGGATG TTCTCTGGGC AGGAACACCC ATGGTGACTA TGCCAGGAGA GACTCTTGCC TCTCGAGTTG CAGCTTCTCA GCTTACTTGT CTAGGATGTC TCGAGCTCAT TGCTAAAAGC AGACAGGAAT ATGAAGACAT AGCTGTGAAA CTGGGAACCG ATCTAGAATA CCTGAAGAAA ATTCGTGGCA AAGTCTGGAA ACAGAGAATA TCTAGCCCTC TGTTCAACAC CAAACAATAC ACAATGGAAT TAGAGCGACT TTATCTGCAG ATGTGGGAGC ATTATGCAGC TGGCAACAAA CCTGACCACA TGATTAAGCC TGTTGAAGTC ACCGAGTCAG CCGAACAAAA ACTCATCTCA GAAGAGGATC TGTAAGGATC CACTAGTCCA GTGTGGTGGA ATTCTGCAGA TATCCAGCAC AGTGGCGGCC GCTCGAGTCT AGAGGGCCCG TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGT
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