pMD18-SCN9A
$142.00
In stock
SKU
PL74852
NM_002977.3
100μg liquid plasmid(Instantly):$163
| name | pMD18-SCN9A | another name | apMD18-SCN9A |
| Insert Size (bp) | 5934bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | pMD18-T | 5′ sequencing primer | M13F： |
| 3′ sequencing primer | M13R： | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA AGAAGCATGA CGGCAAGTGG ACGATCTTGG TACCGAGCTC GGATCCGCCA CCATGGCAAT GTTGCCTCCC CCAGGACCTC AGAGCTTTGT CCATTTCACA AAACAGTCTC TTGCCCTCAT TGAACAACGC ATTGCTGAAA GAAAATCAAA GGAACCCAAA GAAGAAAAGA AAGATGATGA TGAAGAAGCC CCAAAGCCAA GCAGTGACTT GGAAGCTGGC AAACAGCTGC CCTTCATCTA TGGGGACATT CCTCCCGGCA TGGTGTCAGA GCCCCTGGAG GACTTGGACC CCTACTATGC AGACAAAAAG ACTTTCATAG TATTGAACAA AGGGAAAACA ATCTTCCGTT TCAATGCCAC ACCTGCTTTA TATATGCTTT CTCCTTTCAG TCCTCTAAGA AGAATATCTA TTAAGATTTT AGTACACTCC TTATTCAGCA TGCTCATCAT GTGCACTATT CTGACAAACT GCATATTTAT GACCATGAAT AACCCACCGG ACTGGACCAA AAATGTCGAG TACACTTTTA CTGGAATATA TACTTTTGAA TCACTTGTAA AAATCCTTGC AAGAGGCTTC TGTGTAGGAG AATTCACTTT TCTTCGTGAC CCGTGGAACT GGCTGGATTT TGTCGTCATT GTTTTTGCGT ATTTAACAGA ATTTGTAAAC CTAGGCAATG TTTCAGCTCT TCGAACTTTC AGAGTATTGA GAGCTTTGAA AACTATTTCT GTAATCCCAG GCCTGAAGAC AATTGTAGGG GCTTTGATCC AGTCAGTGAA GAAGCTTTCT GATGTCATGA TCCTGACTGT GTTCTGTCTG AGTGTGTTTG CACTAATTGG ACTACAGCTG TTCATGGGAA ACCTGAAGCA TAAATGTTTT CGAAATTCAC TTGAAAATAA TGAAACATTA GAAAGCATAA TGAATACCCT AGAGAGTGAA GAAGACTTTA GAAAATATTT TTATTACTTG GAAGGATCCA AAGATGCTCT CCTTTGTGGT TTCAGCACAG ATTCAGGTCA GTGTCCAGAG GGGTACACCT GTGTGAAAAT TGGCAGAAAC CCTGATTATG GCTACACGAG CTTTGACACT TTCAGCTGGG CCTTCTTAGC CTTGTTTAGG CTAATGACCC AAGATTACTG GGAAAACCTT TACCAACAGA CGCTGCGTGC TGCTGGCAAA ACCTACATGA TCTTCTTTGT CGTAGTGATT TTCCTGGGCT CCTTTTATCT AATAAACTTG ATCCTGGCTG TGGTTGCCAT GGCATATGAA GAACAGAACC AGGCAAACAT TGAAGAAGCT AAACAGAAAG AATTAGAATT TCAACAGATG TTAGACCGTC TTAAAAAAGA GCAAGAAGAA GCTGAGGCAA TTGCAGCGGC AGCGGCTGAA TATACAAGTA TTAGGAGAAG CAGAATTATG GGCCTCTCAG AGAGTTCTTC TGAAACATCC AAACTGAGCT CTAAAAGTGC TAAAGAAAGA AGAAACAGAA GAAAGAAAAA GAATCAAAAG AAGCTCTCCA GTGGAGAGGA AAAGGGAGAT GCTGAGAAAT TGTCGAAATC AGAATCAGAG GACAGCATCA GAAGAAAAAG TTTCCACCTT GGTGTCGAAG GGCATAGGCG AGCACATGAA AAGAGGTTGT CTACCCCCAA TCAGTCACCA CTCAGCATTC GTGGCTCCTT GTTTTCTGCA AGGCGAAGCA GCAGAACAAG TCTTTTTAGT TTCAAAGGCA GAGGAAGAGA TATAGGATCT GAGACTGAAT TTGCCGATGA TGAGCACAGC ATTTTTGGAG ACAATGAGAG CAGAAGGGGC TCACTGTTTG TGCCCCACAG ACCCCAGGAG CGACGCAGCA GTAACATCAG CCAAGCCAGT AGGTCCCCAC CAATGCTGCC GGTGAACGGG AAAATGCACA GTGCTGTGGA CTGCAACGGT GTGGTCTCCC TGGTTGATGG ACGCTCAGCC CTCATGCTCC CCAATGGACA GCTTCTGCCA GAGGGCACGA CCAATCAAAT ACACAAGAAA AGGCGTTGTA GTTCCTATCT CCTTTCAGAG GATATGCTGA ATGATCCCAA CCTCAGACAG AGAGCAATGA GTAGAGCAAG CATATTAACA AACACTGTGG AAGAACTTGA AGAGTCCAGA CAAAAATGTC CACCTTGGTG GTACAGATTT GCACACAAAT TCTTGATCTG GAATTGCTCT CCATATTGGA TAAAATTCAA AAAGTGTATC TATTTTATTG TAATGGATCC TTTTGTAGAT CTTGCAATTA CCATTTGCAT AGTTTTAAAC ACATTATTTA TGGCTATGGA ACACCACCCA ATGACTGAGG AATTCAAAAA TGTACTTGCT ATAGGAAATT TGGTCTTTAC TGGAATCTTT GCAGCTGAAA TGGTATTAAA ACTGATTGCC ATGGATCCAT ATGAGTATTT CCAAGTAGGC TGGAATATTT TTGACAGCCT TATTGTGACT TTAAGTTTAG TGGAGCTCTT TCTAGCAGAT GTGGAAGGAT TGTCAGTTCT GCGATCATTC AGACTGCTCC GAGTCTTCAA GTTGGCAAAA TCCTGGCCAA CATTGAACAT GCTGATTAAG ATCATTGGTA ACTCAGTAGG GGCTCTAGGT AACCTCACCT TAGTGTTGGC CATCATCGTC TTCATTTTTG CTGTGGTCGG CATGCAGCTC TTTGGTAAGA GCTACAAAGA ATGTGTCTGC AAGATCAATG ATGACTGTAC GCTCCCACGG TGGCACATGA ACGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGAGAG TGGATAGAGA CCATGTGGGA CTGTATGGAG GTCGCTGGTC AAGCTATGTG CCTTATTGTT TACATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTAA ACCTATTTCT GGCCTTATTA TTGAGCTCAT TTAGTTCAGA CAATCTTACA GCAATTGAAG AAGACCCTGA TGCAAACAAC CTCCAGATTG CAGTGACTAG AATTAAAAAG GGAATAAATT ATGTGAAACA AACCTTACGT GAATTTATTC TAAAAGCATT TTCCAAAAAG CCAAAGATTT CCAGGGAGAT AAGACAAGCA GAAGATCTGA ATACTAAGAA GGAAAACTAT ATTTCTAACC ATACACTTGC TGAAATGAGC AAAGGTCACA ATTTCCTCAA GGAAAAAGAT AAAATCAGTG GTTTTGGAAG CAGCGTGGAC AAACACTTGA TGGAAGACAG TGATGGTCAA TCATTTATTC ACAATCCCAG CCTCACAGTG ACAGTGCCAA TTGCACCTGG GGAATCCGAT TTGGAAAATA TGAATGCTGA GGAACTTAGC AGTGATTCGG ATAGTGAATA CAGCAAAGTG AGATTAAACC GGTCAAGCTC CTCAGAGTGC AGCACAGTTG ATAACCCTTT GCCTGGAGAA GGAGAAGAAG CAGAGGCTGA ACCTATGAAT TCCGATGAGC CAGAGGCCTG TTTCACAGAT GGTTGTGTAC GGAGGTTCTC ATGCTGCCAA GTTAACATAG AGTCAGGGAA AGGAAAAATC TGGTGGAACA TCAGGAAAAC CTGCTACAAG ATTGTTGAAC ACAGTTGGTT TGAAAGCTTC ATTGTCCTCA TGATCCTGCT CAGCAGTGGT GCCCTGGCTT TTGAAGATAT TTATATTGAA AGGAAAAAGA CCATTAAGAT TATCCTGGAG TATGCAGACA AGATCTTCAC TTACATCTTC ATTCTGGAAA TGCTTCTAAA ATGGATAGCA TATGGTTATA AAACATATTT CACCAATGCC TGGTGTTGGC TGGATTTCCT AATTGTTGAT GTTTCTTTGG TTACTTTAGT GGCAAACACT CTTGGCTACT CAGATCTTGG CCCCATTAAA TCCCTTCGGA CACTGAGAGC TTTAAGACCT CTAAGAGCCT TATCTAGATT TGAAGGAATG AGGGTCGTTG TGAATGCACT CATAGGAGCA ATTCCTTCCA TCATGAATGT GCTACTTGTG TGTCTTATAT TCTGGCTGAT ATTCAGCATC ATGGGAGTAA ATTTGTTTGC TGGCAAGTTC TATGAGTGTA TTAACACCAC AGATGGGTCA CGGTTTCCTG CAAGTCAAGT TCCAAATCGT TCCGAATGTT TTGCCCTTAT GAATGTTAGT CAAAATGTGC GATGGAAAAA CCTGAAAGTG AACTTTGATA ATGTCGGACT TGGTTACCTA TCTCTGCTTC AAGTTGCAAC TTTTAAGGGA TGGACGATTA TTATGTATGC AGCAGTGGAT TCTGTTAATG TAGACAAGCA GCCCAAATAT GAATATAGCC TCTACATGTA TATTTATTTT GTCGTCTTTA TCATCTTTGG GTCATTCTTC ACTTTGAACT TGTTCATTGG TGTCATCATA GATAATTTCA ACCAACAGAA AAAGAAGCTT GGAGGTCAAG ACATCTTTAT GACAGAAGAA CAGAAGAAAT ACTATAATGC AATGAAAAAG CTGGGGTCCA AGAAGCCACA AAAGCCAATT CCTCGACCAG GGAACAAAAT CCAAGGATGT ATATTTGACC TAGTGACAAA TCAAGCCTTT GATATTAGTA TCATGGTTCT TATCTGTCTC AACATGGTAA CCATGATGGT AGAAAAGGAG GGTCAAAGTC AACATATGAC TGAAGTTTTA TATTGGATAA ATGTGGTTTT TATAATCCTT TTCACTGGAG AATGTGTGCT AAAACTGATC TCCCTCAGAC ACTACTACTT CACTGTAGGA TGGAATATTT TTGATTTTGT GGTTGTGATT ATCTCCATTG TAGGTATGTT TCTAGCTGAT TTGATTGAAA CGTATTTTGT GTCCCCTACC CTGTTCCGAG TGATCCGTCT TGCCAGGATT GGCCGAATCC TACGTCTAGT CAAAGGAGCA AAGGGGATCC GCACGCTGCT CTTTGCTTTG ATGATGTCCC TTCCTGCGTT GTTTAACATC GGCCTCCTGC TCTTCCTGGT CATGTTCATC TACGCCATCT TTGGAATGTC CAACTTTGCC TATGTTAAAA AGGAAGATGG AATTAATGAC ATGTTCAATT TTGAGACCTT TGGCAACAGT ATGATTTGCC TGTTCCAAAT TACAACCTCT GCTGGCTGGG ATGGATTGCT AGCACCTATT CTTAACAGTA AGCCACCCGA CTGTGACCCA AAAAAAGTTC ATCCTGGAAG TTCAGTTGAA GGAGACTGTG GTAACCCATC TGTTGGAATA TTCTACTTTG TTAGTTATAT CATCATATCC TTCCTGGTTG TGGTGAACAT GTACATTGCA GTCATACTGG AGAATTTTAG TGTTGCCACT GAAGAAAGTA CTGAACCTCT GAGTGAGGAT GACTTTGAGA TGTTCTATGA GGTTTGGGAG AAGTTTGATC CCGATGCGAC CCAGTTTATA GAGTTCTCTA AACTCTCTGA TTTTGCAGCT GCCCTGGATC CTCCTCTTCT CATAGCAAAA CCCAACAAAG TCCAGCTCAT TGCCATGGAT CTGCCCATGG TTAGTGGTGA CCGGATCCAT TGTCTTGACA TCTTATTTGC TTTTACAAAG CGTGTTTTGG GTGAGAGTGG GGAGATGGAT TCTCTTCGTT CACAGATGGA AGAAAGGTTC ATGTCTGCAA ATCCTTCCAA AGTGTCCTAT GAACCCATCA CAACCACACT AAAACGGAAA CAAGAGGATG TGTCTGCTAC TGTCATTCAG CGTGCTTATA GACGTTACCG CTTAAGGCAA AATGTCAAAA ATATATCAAG TATATACATA AAAGATGGAG ACAGAGATGA TGATTTACTC AATAAAAAAG ATATGGCTTT TGATAATGTT AATGAGAACT CAAGTCCAGA AAAAACAGAT GCCACTTCAT CCACCACCTC TCCACCTTCA TATGATAGTG TAACAAAGCC AGACAAAGAG AAATATGAAC AAGACAGAAC AGAAAAGGAA GACAAAGGGA AAGACAGCAA GGAAAGCAAA AAAGAATTCT GCAGATATCC AGCAATCTCC AGAGGATCGC CGGGAACCGA GGACGAGTTC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCTAA GAAACCATTA TTATCATGAC ATTAACCTAT AAAAATAGGC GTATCACGAG GCCCTTTCGT C
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