pMIR-Report-JUN 3’UTR-△483-734
$99.00
In stock
SKU
PL74781
100μg liquid plasmid(Instantly):$120
| name | pMIR-Report-JUN 3’UTR-△483-734 | another name | apMIR-Report-JUN 3’UTR-△483-734 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Signal report | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pMIR-Report | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC TATGATATCA CGCACGCGTA ACCTCAGCAA CTAAAAATAA AAACTTCAGT ATATAACTTG GGAACAAAAA AGTAGTAAAG GGAAGCAATT CAAGATCTGT TATTGGTATT TGAATACATT TATTGTGACA GAATGCTGTT ATAAATATTC ATAAGACAAA GGCCATCTTT TTATCTAGGA ATTGTCAAAG AGAAGATTCC AAATTGGAAG GATACATCTT TTGTAAAATC TGCCACCAAT TCCTGCTTTG AGAATAAGCA CCTATTGTAA AATTTCTAAC ATTATAAATG GTCACAGCAC ATGCCACTTG ATGCAATCCA AACTTTGAAA TGTTTGACTT CTCAGTGGGC TGTCCCTCTC CACTGCAACC CCCCTTCCTC CAGCCTCCTG AAACATCGCA CTAGCCTTTG GTAAGCAATT CCATATAGAT ACGCTGGGGG AGCGTGGAGT ATGAAGCCTG ACATAGCATC AGGTACATCA GGTACATTTA TTTCTAAGTC TAATAGAGAA CAGTTTTTAC TGCTTAATAG TAAGAAGCAC TGAGAGTGAT TTTATCGAGC ATTCTTAACT CTTTCAACTC CACCTAGATA GACTAGGATC TACAGATGAT GCAGAAAAGA GGTTAGGGGA GTACTTTCCA ATAGTTTATT GTATTTTCTT AAATATCCTT TCTGGAATTT TCAGAAACAA AATCATCTGT AGGCTCAGCC TCGCTCTCAC AAACCTCCCT CCTGCCGCCC CTCCCCAACC CTCCCCCCGC TTTGTGTTCT TAAGGAGTAC TACAGAAGCA ATCTACAGTC TCTATTGCAG TTTGTAACCC CCTCCCCCTC CCCCCTTTAA TACTGAATGA GATCGAATGT TAGGTCCATG CAGTTCTTGG TCAATGTTAA CGAAAGTCCA ACGTTCCGTT CGCGCGGGGA CAGCCCGTCC GCAAAGCGGG GCAGCCCGCA GGCGGCCGCT CCCTGGCTCC ACGCCAAGGG AGGGCGCGAC CAAGTCCTTC CCACTCGTGC ACACTGGGGG CGCCGTCAGA CCGAACCCAG TCCAACTTGG ATACCCTTGG CTTTAGTTCT CGGACACTTC TTTTTTCTCT CCGTCGCAAC TTGTCAAGTT CTCAAGTCTG TCTCTCTGTG TTATTTTTTT TCTTCGTTGC CCCTCAGCCC CCGACGGTCT CTCTTCACTC GAGCAATTTG GACTTTCCGC CCTTCTTGGC CTTTATGAGG ATCTCTCTGA TTTTTCTTGC GTCGAGTTTT CCGGTAAGAC CTTTCGGTAC TTCGTCCACA AACACAACTC CTCCGCGCAA CTTTTTCGCG GTTGTTACTT GACTGGCGAC GTAATCCACG ATCTCTTTTT CCGTCATCGT CTTTCCGTGC TCCAAAACAA CAACGGCGGC GGGAAGTTCA CCGGCGTCAT CGTCGGGAAG ACCTGCCACG CCCGCGTCGA AGATGTTGGG GTGTTGTAAC AATATCGATT CCAATTCAGC GGGGGCCACC TGATATCCTT TGTATTTAAT TAAAGACTTC AAGCGGTCAA CTATGAAGAA GTGTTCGTCT TCGTCCCAGT AAGCTATGTC TCCAGAATGT AGCCATCCAT CCTTGTCAAT CAAGGCGTTG GTCGCTTCCG GATTGTTTAC ATAACCGGAC ATAATCATAG GTCCTCTGAC ACATAATTCG CCTCTCTGAT TAACGCCCAG CGTTTTCCCG GTATCCAGAT CCACAACCTT CGCTTCAAAA AATGGAACAA CTTTACCGAC CGCGCCCGGT TTATCATCCC CCTCGGGTGT AATCAGAATA GCTGATGTAG TCTCAGTGAG CCCATATCCT TGTCGTATCC CTGGAAGATG GAAGCGTTTT GCAACCGCTT CCCCGACTTC TTTCGAAAGA GGTGCGCCCC CAGAAGCAAT TTCGTGTAAA TTAGATAAAT CGTATTTGTC AATCAGAGTG CTTTTGGCGA AGAATGAAAA TAGGGTTGGT ACTAGCAACG CACTTTGAAT TTTGTAATCC TGAAGGGATC GTAAAAACAG CTCTTCTTCA AATCTATACA TTAAGACGAC TCGAAATCCA CATATCAAAT ATCCGAGTGT AGTAAACATT CCAAAACCGT GATGGAATGG GACAACACTT AAAATCGCAG TATCCGGAAC GATTTGATTG CCAAAAATAG GATCTCTGGC ATGCGAGAAT CTGACGCAGG CAGTTCTATG CGGAAGGGCC ACACCCTTAG GTAACCCAGT AGATCCAGAG GAATTCATTA TCAGTGCAAT TGTTTTGTCA CGATCAAAGG ACTCTGGTAC AAAATCGTAT TCATTAAAAC CGGGAGGTAG ATGAGATGTG ACGAACGTGT ACATCGACTG AAATCCCTGG TAATCCGTTT TAGAATCCAT GATAATAATT TTCTGGATTA TTGGTAATTT TTTTTGCACG TTCAAAATTT TTTGCAACCC CTTTTTGGAA ACAAACACTA CGGTAGGCTG CGAAATGTTC ATACTGTTGA GCAATTCACG TTCATTATAA ATGTCGTTCG CGGGCGCAAC TGCAACTCCG ATAAATAACG CGCCCAACAC CGGCATAAAG AATTGAAGAG AGTTTTCACT GCATACGACG ATTCTGTGAT TTGTATTCAG CCCATATCGT TTCATAGCTT CTGCCAACCG AACGGACATT TCGAAGTATT CCGCGTACGT GATGTTCACC TCGATATGTG CATCTGTAAA AGCAATTGTT CCAGGAACCA GGGCGTATCT CTTCATAGCC TTATGCAGTT GCTCTCCAGC GGTTCCATCC TCTAGAGGAT AGAATGGCGC CGGGCCTTTC TTTATGTTTT TGGCGTCTTC CATGGTGGAT CCACGGTTCA CTAAACCAGC TCTGCTTATA TAGACCTCCC ACCGTACACG CCTACCGCCC ATTTGCGTCA ATGGGGCGGA GTTGTTACGA CATTTTGGAA AGTCCCGTTG ATTTTGGTGC CAAAACAAAC TCCCATTGAC GTCAATGGGG TGGAGACTTG GAAATCCCCG TGAGTCAAAC CGCTATCCAC GCCCATTGAT GTACTGCCAA AACCGCATCA CCATGGTAAT AGCGATGACT AATACGTAGA TGTACTGCCA AGTAGGAAAG TCCCATAAGG TCATGTACTG GGCATAATGC CAGGCGGGCC ATTTACCGTC ATTGACGTCA ATAGGGGGCG TACTTGGCAT ATGATACACT TGATGTACTG CCAAGTGGGC AGTTTACCGT AAATACTCCA CCCATTGACG TCAATGGAAA GTCCCTATTG GCGTTACTAT GGGAACATAC GTCATTATTG ACGTCAATGG GCGGGGGTCG TTGGGCGGTC AGCCAGGCGG GCCATTTACC GTAAGTTATG TAACGCGGAA CTCCATATAT GGGCTATGAA CTAATGACCC CGTAATTGAT TACTATTAAT AACTAAGATC TGGTACCTTG AATTCATGCT TCTCCTCCCT TTAGTGAGGG TAATTCTCTC TCTCTCCCTA TAGTGAGTCG TATTAATTCC TTCTCTTCTA TAGTGTCACC TAAATCGTTG CAATTCGTAA TCATGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAAAAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA CCTATAAAAA TAGGCGTATC ACGAGATTGC AGTGAAAAAA ATGCTTTATT TGTGAAATTT GTGATGCTAT TGCTTTATTT GTAACCATTA TAAGCTGCAA TAAACAAGTT AACAACAACA ATTGCATTCA TTTTATGTTT CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCCTCT AGAGTCGGTG GGCCTCGGGG GCGGGTGCGG GGTCGGCGGG GCCGCCCCGG GTGGCTTCGG TCGGAGCCAT GGGGTCGTGC GCTCCTTTCG GTCGGGCGCT GCGGGTCGTG GGGCGGGCGT CAGGCACCGG GCTTGCGGGT CATGCACCAG GTGCGCGGTC CTTCGGGCAC CTCGACGTCG GCGGTGACGG TGAAGCCGAG CCGCTCGTAG AAGGGGAGGT TGCGGGGCGC GGAGGTCTCC AGGAAGGCGG GCACCCCGGC GCGCTCGGCC GCCTCCACTC CGGGGAGCAC GACGGCGCTG CCCAGACCCT TGCCCTGGTG GTCGGGCGAG ACGCCGACGG TGGCCAGGAA CCACGCGGGC TCCTTGGGCC GGTGCGGCGC CAGGAGGCCT TCCATCTGTT GCTGCGCGGC CAGCCGGGAA CCGCTCAACT CGGCCATGCG CGGGCCGATC TCGGCGAACA CCGCCCCCGC TTCGACGCTC TCCGGCGTGG TCCAGACCGC CACCGCGGCG CCGTCGTCCG CGACCCACAC CTTGCCGATG TCGAGCCCGA CGCGCGTGAG GAAGAGTTCT TGCAGCTCGG TGACCCGCTC GATGTGGCGG TCCGGGTCGA CGGTGTGGCG CGTGGCGGGG TAGTCGGCGA ACGCGGCGGC GAGGGTGCGT ACGGCCCGGG GGACGTCGTC GCGGGTGGCG AGGCGCACCG TGGGCTTGTA CTCGGTCATG GAAGGTCGTC TCCTTGTGAG GGGTCAGGGG CGTGGGTCAG GGGATGGTGG CGGCACCGGT CGTGGCGGCC GACCTGCAGG CATGCAAGCT AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCAATCTTTC GTC
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