pMIR-Report-JUN 3’UTR-△401-453
$99.00
In stock
SKU
PL74778
100μg liquid plasmid(Instantly):$120
| name | pMIR-Report-JUN 3’UTR-△401-453 | another name | apMIR-Report-JUN 3’UTR-△401-453 |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Signal report | Fragment type | UTR |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Fluc |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pMIR-Report | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC ACCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCC ATTCGCCATT CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC ACGACGTTGT AAAACGACGG CCAGTGCCAA GCTAGCGGCC GCATACAAAA AACCAACACA CAGATCCAAT GAAAATAAAA GATCCTTTAT TAAGCTTAAC TGTTTAAACC TATGATATCA CGCACGCGTA ACCTCAGCAA CTAAAAATAA AAACTTCAGT ATATAACTTG GGAACAAAAA AGTAGTAAAG GGAAGCAATT CAAGATCTGT TATTGGTATT TGAATACATT TATTGTGACA GAATGCTGTT ATAAATATTC ATAAGACAAA GGCCATCTTT TTATCTAGGA ATTGTCAAAG AGAAGATTCC AAATTGGAAG GATACATCTT TTGTAAAATC TGCCACCAAT TCCTGCTTTG AGAATAAGCA CCTATTGTAA AATTTCTAAC ATTATAAATG GTCACAGCAC ATGCCACTTG ATGCAATCCA AACTTTGAAA TGTTTGACTT CTCAGTGGGC TGTCCCTCTC CACTGCAACC CCCCTTCCTC CAGCCTCCTG AAACATCGCA CTAGCCTTTG GTAAGCAATT CCATATAGAT ACGCTGGGGG AGCGTGGAGT ATGAAGCCTG ACATAGCATC AGGTACATCA GGTACATTTA TTTCTAAGTC TAATAGAGAA CAGTTTTTAC TGCTTAATAG TAAGAAGCAC TGAGAGTGAT TTTATCGAGC ATTCTTAACT CTTTCAACTC CACCTAGATA GACTAGGATC TACAGATGAT GCAGAAAAGA GGTTAGGGGA GTACTTTCCA ATAGTTTATT GTATTTTCTT AAATATCCTT TCTGGAATTT TCAGAAACAA AACATAAAAA AATTATATAC TTTATTACAA ATGGTAAACT CAGAGTGCTC CAAATCTCTT ATTTACAAAC AACACTGGGC AGGATACCCA AACAAACAAA CAAGAAATAA CTTACAAAGG CATGAAGCTG TTTATTGACA GTAATCAGCT TTAATCAAAT TAAAAAATAT ATATATGTAC ATACACAGTT AACGAAAGCA GGCCAGAAAG AGTTCATCTG TAGGCTCAGC CTCGCTCTCA CATGTTCTTA AGGAGTACTA CAGAAGCAAT CTACAGTCTC TATTGCAGTT TGTAACCCCC TCCCCCTCCC CCCTTTAATA CTGAATGAGA TCGAATGTTA GGTCCATGCA GTTCTTGGTC AATGTTAACG AAAGTCCAAC GTTCCGTTCG CGCGGGGACA GCCCGTCCGC AAAGCGGGGC AGCCCGCAGG CGGCCGCTCC CTGGCTCCAC GCCAAGGGAG GGCGCGACCA AGTCCTTCCC ACTCGTGCAC ACTGGGGGCG CCGTCAGACC GAACCCAGTC CAACTTGGAT ACCCTTGGCT TTAGTTCTCG GACACTTCTT TTTTCTCTCC GTCGCAACTT GTCAAGTTCT CAAGTCTGTC TCTCTGTGTT ATTTTTTTTC TTCGTTGCCC CTCAGCCCCC GACGGTCTCT CTTCACTCGA GCAATTTGGA CTTTCCGCCC TTCTTGGCCT TTATGAGGAT CTCTCTGATT TTTCTTGCGT CGAGTTTTCC GGTAAGACCT TTCGGTACTT CGTCCACAAA CACAACTCCT CCGCGCAACT TTTTCGCGGT TGTTACTTGA CTGGCGACGT AATCCACGAT CTCTTTTTCC GTCATCGTCT TTCCGTGCTC CAAAACAACA ACGGCGGCGG GAAGTTCACC GGCGTCATCG TCGGGAAGAC CTGCCACGCC CGCGTCGAAG ATGTTGGGGT GTTGTAACAA TATCGATTCC AATTCAGCGG GGGCCACCTG ATATCCTTTG TATTTAATTA AAGACTTCAA GCGGTCAACT ATGAAGAAGT GTTCGTCTTC GTCCCAGTAA GCTATGTCTC CAGAATGTAG CCATCCATCC TTGTCAATCA AGGCGTTGGT CGCTTCCGGA TTGTTTACAT AACCGGACAT AATCATAGGT CCTCTGACAC ATAATTCGCC TCTCTGATTA ACGCCCAGCG TTTTCCCGGT ATCCAGATCC ACAACCTTCG CTTCAAAAAA TGGAACAACT TTACCGACCG CGCCCGGTTT ATCATCCCCC TCGGGTGTAA TCAGAATAGC TGATGTAGTC TCAGTGAGCC CATATCCTTG TCGTATCCCT GGAAGATGGA AGCGTTTTGC AACCGCTTCC CCGACTTCTT TCGAAAGAGG TGCGCCCCCA GAAGCAATTT CGTGTAAATT AGATAAATCG TATTTGTCAA TCAGAGTGCT TTTGGCGAAG AATGAAAATA GGGTTGGTAC TAGCAACGCA CTTTGAATTT TGTAATCCTG AAGGGATCGT AAAAACAGCT CTTCTTCAAA TCTATACATT AAGACGACTC GAAATCCACA TATCAAATAT CCGAGTGTAG TAAACATTCC AAAACCGTGA TGGAATGGGA CAACACTTAA AATCGCAGTA TCCGGAACGA TTTGATTGCC AAAAATAGGA TCTCTGGCAT GCGAGAATCT GACGCAGGCA GTTCTATGCG GAAGGGCCAC ACCCTTAGGT AACCCAGTAG ATCCAGAGGA ATTCATTATC AGTGCAATTG TTTTGTCACG ATCAAAGGAC TCTGGTACAA AATCGTATTC ATTAAAACCG GGAGGTAGAT GAGATGTGAC GAACGTGTAC ATCGACTGAA ATCCCTGGTA ATCCGTTTTA GAATCCATGA TAATAATTTT CTGGATTATT GGTAATTTTT TTTGCACGTT CAAAATTTTT TGCAACCCCT TTTTGGAAAC AAACACTACG GTAGGCTGCG AAATGTTCAT ACTGTTGAGC AATTCACGTT CATTATAAAT GTCGTTCGCG GGCGCAACTG CAACTCCGAT AAATAACGCG CCCAACACCG GCATAAAGAA TTGAAGAGAG TTTTCACTGC ATACGACGAT TCTGTGATTT GTATTCAGCC CATATCGTTT CATAGCTTCT GCCAACCGAA CGGACATTTC GAAGTATTCC GCGTACGTGA TGTTCACCTC GATATGTGCA TCTGTAAAAG CAATTGTTCC AGGAACCAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG TTCCATCCTC TAGAGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA TGGTGGATCC ACGGTTCACT AAACCAGCTC TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT AATGACCCCG TAATTGATTA CTATTAATAA CTAAGATCTG GTACCTTGAA TTCATGCTTC TCCTCCCTTT AGTGAGGGTA ATTCTCTCTC TCTCCCTATA GTGAGTCGTA TTAATTCCTT CTCTTCTATA GTGTCACCTA AATCGTTGCA ATTCGTAATC ATGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA AAAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGATTGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT GATCCTCTAG AGTCGGTGGG CCTCGGGGGC GGGTGCGGGG TCGGCGGGGC CGCCCCGGGT GGCTTCGGTC GGAGCCATGG GGTCGTGCGC TCCTTTCGGT CGGGCGCTGC GGGTCGTGGG GCGGGCGTCA GGCACCGGGC TTGCGGGTCA TGCACCAGGT GCGCGGTCCT TCGGGCACCT CGACGTCGGC GGTGACGGTG AAGCCGAGCC GCTCGTAGAA GGGGAGGTTG CGGGGCGCGG AGGTCTCCAG GAAGGCGGGC ACCCCGGCGC GCTCGGCCGC CTCCACTCCG GGGAGCACGA CGGCGCTGCC CAGACCCTTG CCCTGGTGGT CGGGCGAGAC GCCGACGGTG GCCAGGAACC ACGCGGGCTC CTTGGGCCGG TGCGGCGCCA GGAGGCCTTC CATCTGTTGC TGCGCGGCCA GCCGGGAACC GCTCAACTCG GCCATGCGCG GGCCGATCTC GGCGAACACC GCCCCCGCTT CGACGCTCTC CGGCGTGGTC CAGACCGCCA CCGCGGCGCC GTCGTCCGCG ACCCACACCT TGCCGATGTC GAGCCCGACG CGCGTGAGGA AGAGTTCTTG CAGCTCGGTG ACCCGCTCGA TGTGGCGGTC CGGGTCGACG GTGTGGCGCG TGGCGGGGTA GTCGGCGAAC GCGGCGGCGA GGGTGCGTAC GGCCCGGGGG ACGTCGTCGC GGGTGGCGAG GCGCACCGTG GGCTTGTACT CGGTCATGGA AGGTCGTCTC CTTGTGAGGG GTCAGGGGCG TGGGTCAGGG GATGGTGGCG GCACCGGTCG TGGCGGCCGA CCTGCAGGCA TGCAAGCTAG CTTTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC AATCTTTCGT C
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