pCDNA3.1-MAGEL2-6×His
$113.00
In stock
SKU
PL74734
NM_019066.5;NDNL1;nM15;PWLS;SHFYNG
100μg liquid plasmid(Instantly):$134
| name | pCDNA3.1-MAGEL2-6×His | another name | apCDNA3.1-MAGEL2-6×His |
| Insert Size (bp) | 3747bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA3.1 | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA TGTCGCAGCT AAGTAAGAAT CTGGGTGACT CGAGTCCTCC GGCGGAGGCC CCGAAGCCGC CTGTCTATAG CCGCCCTACG GTTCTGATGC GGGCCCCGCC CGCTTCCTCC CGGGCTCCGC CAGTCCCTTG GGATCCACCT CCAATTGACT TGCAGGCTTC ATTGGCCGCT TGGCAGGCAC CTCAGCCTGC CTGGGAGGCC CCACAGGGCC AGCTGCCCGC CCCGGTGGTT CCGATGACCC AGCCTCCTGC CCTAGGGGGC CCGATAGTCC CGGCTCCCCC GCTGGGGGGC CCGATGGGTA AGCCTCCGAC TCCCGGGGTC CTGATGGTGC ATCCTCCACC TCCGGGAGCC CCGATGGCCC AGCCTCCGAC CCCGGGAGTC CTGATGGTGC ATCCTTCAGC TCCCGGAGCT CCCATGGCCC ATCCTCCTCC TCCGGGGACC CCAATGTCCC ACCCTCCCCC TCCGGGGACC CCAATGGCCC ATCCTCCTCC TCCGGGGACC CCGATGGCCC ATCCTCCTCC TCCGGGGACC CCGATGGTGC ATCCTCCTCC TCCGGGGACC CCGATGGCTC ATCCTCCCCC TCCGGGGACA CCGATGGCTC ATCCTCCCCC TCCGGGGACA CCGATGGCTC ATCCTCCACC TCCGGGGACA CCGATGGCTC ATCCTCCCCC TCCGGGTACA CCGATGGCCC AGCCTCCAGC TCCGGGAGTC CTGATGGCCC AGCCTCTGAC TCCGGGAGTC CTGATGGTCC AGCCTGCTGC TCCGGGAGCA CCGATGGTCC AGCCGCCTCC AGCAGCCATG ATGACCCAGC CTCAGCCTTC AGGAGCACCG ATGGCCAAGC CTCCAGGTCC AGGAGTCCTG ATGATTCATC CTCCAGGTGC GAGAGCTCCG ATGACCCAGC CTCCAGCTTC AGGAGCACCG ATGGCACAGC CGGCGGCCCC ACCTGCACAG CCGATGGCCC CACCTGCACA GCCGATGGCT TCTTGGGCCC CGCAGGCTCA GCCTCTGATC CTGCAAATCC AGTCTCAAGT TATAAGGGCT CCTCCGCAGG TTCCCCAGGG CCCGCAGGCA CCCCCAGCGC AGCTAGCCAC ACCCCCGGGC TGGCAGGCGA CCTCGCCAGG ATGGCAGGCC ACGCAGCAAG GCTGGCAGGC CACTCCCCTG ACTTGGCAGA CCACGCAGGT CACCTGGCAG GCACCAGCCG TTACCTGGCA GGTGCCGCCG CCCATGCGCC AGGGGCCCCC GCCCATCCGC CCTGGCCCAC CACCCATCCG CCCTGGCCCA CCACCGGTGC GACAGGCCCC ACCGCTGATC CGCCAGGCCC CACCGGTGAT CCGCCAGGCC CCACCCGTGA TCCGCCAGGC CCCACCCGTG ATCCGCCAGG CCCCCGCTGT GATCCGCCAG GCCCCACCTG TGATCCGCCA GGCCCCACCT GTGATCCGCC AGGCTCCACC TGTGATCCGC CAGGCCCCGC CGCTGATCCG CCAGGCGCCG CCGCCCATCC GACCTGCCCC ACAGGTCCTG GCCACCCAGC CACCGCTCTG GCAGGCCCTG CCACCCCCAC CTCCACTGCG GCAGGCCCCG CAGGCTAGGC TGCCGGCCCC GCAGGTGCAG GCGGCGCCGC AGGTGCCTAC GGCCCCACCT GCTACGCAGG TACCCGCGGC GCCGCCCGCT GGCCCGCAGG TGCCCCAGCC TGTGCTGCCG GCCCCGCTGT CTGCCCCACT GTCTGCCCCG CAGGCTGTGC ACTGCCCTTC CATCATCTGG CAGGCCCCCA AAGGTCAGCC CCCGGTGCCA CACGAGATTC CAACGTCAAT GGAATTCCAG GAGGTGCAGC AGACACAGGC GCTGGCCTGG CAGGCCCAGA AGGCCCCCAC TCACATCTGG CAGCCCCTGC CTGCCCAGGA GGCCCAGAGG CAGGCTCCCC CCTTGGTCCA GCTGGAGCAG CCCTTTCAGG GAGCCCCGCC CTCCCAAAAA GCCGTGCAAA TCCAGCTACC CCCCCAGCAG GCCCAGGCAT CGGGTCCGCA AGCGGAGGTG CCCACACTGC CGCTCCAGCC TTCCTGGCAG GCACCGCCTG CAGTCTTGCA GGCCCAGCCC GGACCCCCGG TAGCAGCGGC AAATTTTCCC CTGGGCTCCG CTAAATCATT GATGACTCCA TCAGGAGAAT GCAGGGCCTC TTCTATAGAC CGCAGGGGCT CCTCTAAAGA GCGCAGGACC TCCTCGAAGG AGCGCAGGGC CCCTTCAAAA GACCGCATGA TCTTTGCTGC CACCTTCTGT GCTCCCAAGG CAGTGTCAGC TGCGCGAGCA CACCTGCCAG CTGCCTGGAA AAACCTGCCT GCCACACCGG AGACCTTTGC TCCCTCCTCA AGTGTCTTCC CAGCTACCTC CCAGTTTCAG CCTGCCTCTC TGAATGCCTT TAAAGGCCCC TCTGCTGCCT CAGAGACCCC AAAGTCACTG CCATATGCTC TGCAGGATCC CTTTGCCTGT GTAGAGGCCC TGCCTGCAGT TCCATGGGTC CCACAGCCCA ATATGAATGC CTCAAAGGCA TCGCAGGCAG TGCCCACCTT CCTGATGGCT ACAGCAGCTG CCCCCCAGGC AACTGCCACC ACTCAAGAGG CCTCCAAGAC CTCCGTCGAG CCGCCACGCC GCTCCGGCAA GGCCACCCGG AAGAAGAAGC ATCTGGAAGC CCAAGAGGAC AGCCGTGGCC ACACGCTAGC CTTTCATGAC TGGCAGGGCC CAAGGCCCTG GGAGAATCTA AATCTGAGTG ACTGGGAGGT CCAAAGCCCT ATCCAGGTCT CGGGTGACTG GGAGCACCCA AACACCCCCC GTGGCCTGAG TGGTTGGGAG GGCCCTAGCA CCTCCAGGAT CCTGAGTGGC TGGGAAGGGC CCAGCGCATC CTGGGCCCTG AGTGCCTGGG AGGGCCCGAG CACCTCCAGG GCCCTGGGTC TCTCTGAAAG CCCAGGGAGC TCTCTGCCCG TAGTTGTGTC TGAGGTCGCA AGTGTCTCTC CGGGATCCAG TGCCACCCAG GATAATTCCA AGGTGGAGGC ACAGCCCTTG TCTCCCTTGG ATGAGAGGGC AAATGCGTTG GTGCAGTTCC TCTTAGTCAA GGACCAAGCC AAGGTGCCTG TCCAGCGCTC GGAGATGGTG AAAGTCATCC TCCGAGAGTA TAAAGATGAG TGCTTAGATA TCATCAACCG TGCCAACAAT AAGCTGGAGT GTGCCTTTGG TTATCAATTG AAAGAAATTG ATACCAAAAA CCACGCCTAT ATTATCATCA ACAAGCTGGG CTACCATACA GGGAATTTGG TGGCATCCTA TTTAGACAGG CCCAAGTTTG GCCTTCTGAT GGTGGTCTTG AGCCTCATCT TTATGAAAGG CAACTGTGTC AGGGAGGATC TGATCTTTAA TTTTCTGTTC AAGTTAGGGT TGGATGTCCG GGAGACAAAC GGTCTCTTTG GAAATACTAA GAAGCTCATC ACCGAAGTGT TTGTCAGGCA GAAGTACCTA GAGTACAGGC GAATCCCTTA CACTGAGCCC GCAGAGTATG AGTTCCTCTG GGGCCCTCGA GCATTCCTGG AAACCAGCAA GATGCTTGTC CTGAGGTTTT TGGCCAAGCT CCATAAGAAA GATCCACAGA GCTGGCCATT CCATTACCTT GAAGCGCTCG CAGAGTGTGA GTGGGAAGAC ACAGATGAGG ATGAACCTGA CACCGGTGAC AGTGCCCACG GCCCCACCAG CAGGCCCCCT CCCCGCGCGG CCGCACATCA CCATCATCAC CATTGACTCG AGTCTAGAGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT
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