pCMV-SPORT6-AACS(human)
$84.00
In stock
SKU
PL74357
NM_023928.5;ACSF1;SUR-5
100μg liquid plasmid(Instantly):$105
| name | pCMV-SPORT6-AACS(human) | another name | apCMV-SPORT6-AACS(human) |
| Insert Size (bp) | 2019bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCMV-SPORT6 | 5′ sequencing primer | M13R：CAGGAAACAGCTATGACC |
| 3′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ACCGAACGAC TACTATAGGG ACCACTTTGT ACAAGAAAGC TGGGTACGCG TAAGCTTGGG CCCCTCGAGG GATACTCTAG AGCGGCCGCC CTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTGGG TGCATGTGTC ACACATGTAT ACATTTATTG CATAAAACTC ATCATAGCAC TTTCCCCCAT ATTTTTATAA TCCAAAAGGA AAATGATTCA AGAAAGGATT TCATTGTGCT CAGTTTCAAA AAATATAAAA ATGGACATCA GATTAGAGAT ACAAGTTCAT ACGCTGAACT GAATTGTACA TACCAACTGC CTGGCTATGG AAACCCGTGA CTTGACTTAG GGGTGCTGAT GACATGATCT CGACAAGAAC CCCCTAGCAA CTCTCAGGTG GAGGCAGCAC AGGGATGCGG TTCCTGGTGA GGAGGGTCCT CACTCGGTGA CCACACTGCC TGGGCTCACA GCTGGAGGGC TCACCCATGA GGGACACGGG TGGACACCCA CTGCTTCACA TGCCTAATTC ACCATTAGAA ACATGTAAAG CCATTCAGTC TGTGCAATAA AGAGATCCTG TATGAAATCC ACTCATTCCT TGGAGGGAAG CTGGCCCGGA GGCACGCTCT GGTTGACGGG TGACGCACAG TCCTCCAGGG CCTGCATTGC ATCCATGACA CAGACACACG TGAACACCCA GCCCGCCGGT CCTAGCAGCC AGCTGTGAAA GCTGTGTCAA GTCACGGGGG TTCCTTTCTA ACAGCTAAAG CTCAAAGGCA TACTGGACCC TGAGGGAAAA CAGGAGAGCC TGAGATGGGA AGGGAAGAAA GGGATTTGCA CCTTCACAGA GCAAAATGGG ACAACGGGAC GGGGAGGTTG AAGACAAGAC CTTGGCTCAG GAGTGTGTCT GTCTTCAGCA CCAGCCAGCC CCACCGTTCC CACTGCACTG TGTGTGCAAA GACACACACT CTCACCACAG TGCCACACCT GCGGCCCAGA CACTCCCTCC TGGTCTCTGA AGGCCAGAAT CCCACTCGTG TGGAAGATCC AGGTACCCAC CAGATTTAAC AAAACAGAGA AACGATCCCT CCCCAAGCCT ACAGAACACT TGGTGCGAGC ATCCTTTTAC AACAGCTGTA GGTTTCTGTA TAATTTCTTG AGCACACAAA AGTTACAGTG CACACGGGTG CGGCTGAGTG ACACGCCAGC CAGTCTGACT CAGAAGCCCT GCAGCTCAGG GATGTCCCGG TACAGATCCA GGGTCTCGGG GTTCGAGAAA GCACCTCCTT GCTCCACGGC TTTTCCAGCG ATGATCTGTT TGACGGCAAC TTCCACTTTC TTGCCGTTGA GCGTATACGG GATGCCCTTG GTTTCCAGGA TGAGGCTGGG CACGTGTCGC GCAGACAAGC CCATGCGGAT GGCGTCACGG ATCCTCTTAA CCAAGTCAGG CTGGAAGGCG TGCCCGGAGG CCATCTTCAG GAAGAGGATC ACCCTCTCCT CCCTGTACTT GTTATACTGG GGGACACACA GGCTGTCCTC CACCTCCTCG AAGGATTCCA CAATGTTATA GATTTCCGAG CTGCCGAACC GCACCCCGTT GGGGTTGAGG GTGCCGTCAC TCCGGCCAAG CATGACGATG CCCCCGGTCT TGGGGTTGAT TCTGCAGTAG TCGCCATGAG CCCAGATACC TGGGAATTTG GAGAAATACG CCTTCCTGTA CTTGTTGCCG TTCTCATCGT TCCAGAAGTG TGTGGGCTGG CAAGGGATCG GCTTAGTACA CACCAGCTCG CCGCTCTCTC CCCAGACCGC CTTTCCTTCC TCGTTCCACG CTTCCACGGC CATGCCCAGG TTCCGGGCCT GAATCTCCCC TTTATACACA GGAAGAGAAA AATTGTGGCC CATGAAGCAG GAGATGATGT CGGTGCCTCC TGAGATGGAG CCCAGGAGGA TGCTGCTCTT GATGCACCTG TAGACATACT CGTAGCTCTG GGCTTTCAGT GGGGAGCCAG TGGACAGGAT CGTGTGGAGC ATCTGGAGAC TGTGGGTTTC CACCGGCTTC ATGGCCTTCT CTTCCAGCAC TGACAGCCAC TTGGCCCCAG TTACCAGGAC AGTGATGCCT ATCCTGTCAA CCAGGTCCCA GAGCACATTG GGCGTGGGCA CCAGGGGGGA GCCATCGTAC AAGACCATGG CCGCTCCTGT GGCCAGAAGG GACACCATCC AGTTCCACAT CATCCAGCCG ACCGTGGTGT AGCACAGGAG GATGTCACTG CTGGTCATGT TGCCGTGCAG CAGGTGCTCC TTCAGATGCT GGATGAGGGT GCCCCCAGCG GAATGCACCA TGCACTTGGG TGCGCCCGTG GTGCCCGATG AGAACATGAT GAACAGTGGG TGGCTGAAGG GCAGCTGCTC GAACTCCAGC TGCGGGGCCT GCTCACTGGT GCCGGTGGCA AGAAAGTCAT CCAGAAACAC ACTGTTTGGA ATCTTTGAAA GGTCTATGTT CTCTCTGGAG GACACATAAG GAATCACCAC CACTTTCTTC AAGTCTGGTA GGCCTTTAAC CACCTGCTGC AGCTTTTCCA TGTGGTTGTG CTCTTTGCCA TTATAGACAA CAGCCTCCAC AGAGAAGATG AGCTTTGGCT GAATTTGAGA AAACCGGTCC AGCACACCAT TCACACCGAA GTCCGGGGAC GTGGAGCTCC AGATGGCACC AATGCTTGCC GCAGCCAGCA TCGCCTCGAC AGCGTGCTCA CTGTTGGGTA AATAACCAAC AACCCGATCT CCTTTCTTCA CACCCATTTT CCTCATTGCT GCTGCAAACA AAGCCACTTC TTGCCTCAGC TCTTCAAAAG TCACCTTCAC AATTTCCTCT TTGCCTTCCC TTGCAATGTA AAGGGCAACT CTGTCATTCT CTTTGTGCCG CAGGAGGTTT TCTGCATAGT TGAGCCGACT GCCTTTGAAC CACTCGGGGA CATCTGCGAT TCCTTTCGAT GTGTCCACAA CCTCATCATA CACACGTGAG AAGACAATTC CACTGAATTT CCAGAACTCT GCCCAGAAGT CTGAATATGA CTCAACGGAC CAATGGTACA AGTCATCATA ACTCTCCAGC GCCAGGCCGC AGGCGGCGCC CACAGCCGCC CGGAAGCGGT CCATCTGCGT GTTCTTCTTA CTGTCAGGCT CCCACATCAC CTGGCACTCC AGGATCTCCT CCCGACCGGG GCGCTCCTCC TTGGACATGG CGGCGGCGGG CGGCCGGGGC TGCGACGAGG GCTGGGGACC AGGGGCCGGG GCTGAGGCGA GGGCCGGGGA CCTGGGACCG GAGGAAGCGA CGGTCAGGAG CGCCTGGCGG GCTGGGTCAG CGATCCCGGG AATTCCGGAC CGGTACCAGC CTGCTTTTTT GTACCAAACT GTCTATAGTC ACCTATGCC
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