pGH-SP-IAic-3L
$70.00
In stock
SKU
PL74296
U17696.1
100μg liquid plasmid(Instantly):$91
| name | pGH-SP-IAic-3L | another name | apGH-SP-IAic-3L |
| Insert Size (bp) | 1911bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | lactic acid bacteria | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pGH | 5′ sequencing primer | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTAAATTGTA AGCGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA GGGCGCGTCC CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT TGGGTACGGC CGTCAAGGCC AAGCTTCCCG GGATAGATCT AGTCTTATAA CTATACTGAC AATAGAAACA TTAACAAATC TAAAACAGTC TTAATTCTAT CTTGAGAAAG TATTGGTAAT AATATTATTG TCGATAACGC GAGCATAATA AACGGCTCTG ATTAAATTCT GAAGTTTGTT AGATACAATG ATTTCGTTCG AAGGAACTAC AAAATAAATT ATAAGGAGGC ACTCACCATG AAAAAAAAGA TTATCTCAGC TATTTTAATG TCTACAGTGA TACTTTCTGC TGCAGCCCCG TTGTCAGGTG TTTACGCTGC ATGCGCTCGT CAACAAGACA AGGAACGTCT TGCTGCTCTT GGTCCAGAAG GTGCTCACGG TGACACTACT TTCGAATACC AAGACCTTTG TCGTCAACAC ATGGCTACTA AGTCACTTTT CAACCGTGCT GAAGGTCCAC CAGAACCATC ACGTGTTTCA TCAGTTTCAT CACAATTCTC AGACGCTGCT CAAGCTTCAC CATCATCACA CTCATCAACT CCATCATGGT GTGAAGAACC AGCTCAAGCT AACATGGACA TTTCAACTGG TCACATGATT CTTGCTTACA TGGAAGACCA CCTTCGTAAC CGTGACCGTC TTGCTAAGGA ATGGCAAGCT CTTTGTGCTT ACCAAGCTGA ACCAAACACT TGTGCTACTG CTCAAGGTGA AGGTAACATT AAGAAGAACC GTCACCCAGA CTTCCTTCCA TACGACCACG CTCGTATTAA GCTTAAGGTT GAATCATCAC CATCACGTTC AGACTACATT AACGCTTCAC CAATTATTGA ACACGACCCA CGTATGCCAG CTTACATTGC TACTCAAGGT CCACTTTCAC ACACTATTGC TGACTTCTGG CAAATGGTTT GGGAATCAGG TTGTACTGTT ATTGTTATGC TTACTCCACT TGTTGAAGAC GGTGTTAAGC AATGTGACCG TTACTGGCCA GACGAAGGTG CTTCACTTTA CCACGTTTAC GAAGTTAACC TTGTTTCAGA ACACATTTGG TGTGAAGACT TCCTTGTTCG TTCATTCTAC CTTAAGAACG TTCAAACTCA AGAAACTCGT ACTCTTACTC AATTCCACTT CCTTTCATGG CCAGCTGAAG GTACTCCAGC TTCAACTCGT CCACTTCTTG ACTTCCGTCG TAAGGTTAAC AAGTGTTACC GTGGTCGTTC ATGTCCAATT ATTGTTCACT GTTCAGACGG TGCTGGTCGT ACTGGTACTT ACATTCTTAT TGACATGGTT CTTAACCGTA TGGCTAAGGG TGTTAAGGAA ATTGACATTG CTGCTACTCT TGAACACGTT CGTGACCAAC GTCCAGGTCT TGTTCGTTCA AAGGACCAAT TCGAATTTGC TCTTACTGCT GTTGCTGAAG AAGTTAACGC TATTCTTAAG GCTCTTCCAC AAGGTACCGG AGCTTCTTCA GCTGGAAATA CTAATTCTGG TGGCTCGACA ACCACAATTA CGAATAATAA TTCTGGAACC AATAGCAGTT CAACTACTTA TACCGTCAAA TCTGGTGATA CTCTTTGGGG AATCTCACAA AGATATGGAA TTAGTGTCGC TCAAATTCAA AGTGCGAATA ATCTTAAAAG TACCATTATC TACATTGGTC AAAAACTTGT ACTGACAGGT TCAGCTTCTT CTACAAATTC AGGTGGTTCA AACAATTCCG CAAGCACTAC TCCAACCACT TCTGTGACAC CTGCAAAACC AACTTCACAA ACAACTGTTA AGGTTAAATC CGGAGATACC CTTTGGGCGC TATCAGTAAA ATATAAAACT AGTATTGCTC AATTGAAAAG TTGGAATCAT TTAAGTTCAG ATACCATTTA TATTGGTCAA AATCTTATTG TTTCACAATC TGCTGCTGCT TCAAATCCTT CGACAGGTTC AGGCTCAACT GCTACCAATA ACTCAAACTC GACTTCTTCT AACTCAAATG CCTCAATTCA TAAGGTCGTT AAAGGAGATA CTCTCTGGGG ACTTTCGCAA AAATCTGGCA GCCCAATTGC TTCAATCAAG GCTTGGAATC ATTTATCTAG CGATACTATT TTAATTGGTC AGTATCTACG AATAAAACAT CATCACCATC ACCATTAGTA ATCTAGAATC ACGTGAAGCT TGCAAGCTCC AGCTTTTGTT CCCTTTAGTG AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGATCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
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