pHT1469
$128.00
In stock
SKU
PL74014
pHT-1469
100μg liquid plasmid(Instantly):$149
| name | pHT1469 | another name | apHT1469 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Chl | Fluorescent labeling | |
| Promoter | Pgrac | Replicon | pUC,repB |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | pHT-R：agcaagtcatctccaaattctt | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCATGCTT AAGTTATTGG TATGACTGGT TTTAAGCGCA AAAAAAGTTG CTTTTTCGTA CCTATTAATG TATCGTTAGA AAACCGACTG TAAAAAGTAC AGTCGGCATT ATCTCATATT ATAAAAGCCA GTCATTAGGC CTATCTGACA ATTCCTGAAT AGAGTTCATA AACAATCCTG CATGATAACC ATCACAAACA GAATGATGTA CCTGTAAAGA TAGCGGTAAA TATATTGAAT TACCTTTATT AATGAATTTT CCTGCTGTAA TAATGGGTAG AAGGTAATTA CTATTATTAT TGATATTTAA GTTAAACCCA GTAAATGAAG TCCATGGAAT AATAGAAAGA GAAAAAGCAT TTTCAGGTAT AGGTGTTTTG GGAAACAATT TCCCCGAACC ATTATATTTC TCTACATCAG AAAGGTATAA ATCATAAAAC TCTTTGAAGT CATTCTTTAC AGGAGTCCAA ATACCAGAGA ATGTTTTAGA TACACCATCA AAAATTGTAT AAAGTGGCTC TAACTTATCC CAATAACCTA ACTCTCCGTC GCTATTGTAA CCAGTTCTAA AAGCTGTATT TGAGTTTATC ACCCTTGTCA CTAAGAAAAT AAATGCAGGG TAAAATTTAT ATCCTTCTTG TTTTATGTTT CGGTATAAAA CACTAATATC AATTTCTGTG GTTATACTAA AAGTCGTTTG TTGGTTCAAA TAATGATTAA ATATCTCTTT TCTCTTCCAA TTGTCTAAAT CAATTTTATT AAAGTTCATT TGATATGCCT CCTAAATTTT TATCTAAAGT GAATTTAGGA GGCTTACTTG TCTGCTTTCT TCATTAGAAT CAATCCTTTT TTAAAAGTCA ATATTACTGT AACATAAATA TATATTTTAA AAATATCCCA CTTTATCCAA TTTTCGTTTG TTGAACTAAT GGGTGCTTTA GTTGAAGAAT AAAAGACCAC ATTAAAAAAT GTGGTCTTTT GTGTTTTTTT AAAGGATTTG AGCGTAGCGA AAAATCCTTT TCTTTCTTAT CTTGATAATA AGGGTAACTA TTGCCGTCGT CCATTCCGAC AGCATCGCCA GTCACTATGG CGTGCTGCTA GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCCAGT GAATTCGAGC TCAGGCCTTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCCAGGG TGGTTTTTCT TTTCACCAGT GAGACGGGCA ACAGCTGATT GCCCTTCACC GCCTGGCCCT GAGAGAGTTG CAGCAAGCGG TCCACGCTGG TTTGCCCCAG CAGGCGAAAA TCCTGTTTGA TGGTGGTTAA CGGCGGGATA TAACATGAGC TGTCTTCGGT ATCGTCGTAT CCCACTACCG AGATATCCGC ACCAACGCGC AGCCCGGACT CGGTAATGGC GCGCATTGCG CCCAGCGCCA TCTGATCGTT GGCAACCAGC ATCGCAGTGG GAACGATGCC CTCATTCAGC ATTTGCATGG TTTGTTGAAA ACCGGACATG GCACTCCAGT CGCCTTCCCG TTCCGCTATC GGCTGAATTT GATTGCGAGT GAGATATTTA TGCCAGCCAG CCAGACGCAG ACGCGCCGAG ACAGAACTTA ATGGGCCCGC TAACAGCGCG ATTTGCTGGT GACCCAATGC GACCAGATGC TCCACGCCCA GTCGCGTACC GTCTTCATGG GAGAAAATAA TACTGTTGAT GGGTGTCTGG TCAGAGACAT CAAGAAATAA CGCCGGAACA TTAGTGCAGG CAGCTTCCAC AGCAATGGCA TCCTGGTCAT CCAGCGGATA GTTAATGATC AGCCCACTGA CGCGTTGCGC GAGAAGATTG TGCACCGCCG TTTTACAGGC TTCGACGCCG CTTCGTTCTA CCATCGACAC CACCACGCTG GCACCCAGTT GATCGGCGCG AGATTTAATC GCCGCGACAA TTTGCGACGG CGCGTGCAGG GCCAGACTGG AGGTGGCAAC GCCAATCAGC AACGACTGTT TGCCCGCCAG TTGTTGTGCC ACGCGGTTGG GAATGTAATT CAGCTCCGCC ATCGCCGCTT CCACTTTTTC CCGCGTTTTC GCAGAAACGT GGCTGGCCTG GTTCACCACG CGGGAAACGG TCTGATAAGA GACACCGGCA TACTCTGCGA CATCGTATAA CGTTACTGGT TTCATCAAAA TCGTCTCCCT CCGTTTGAAT ATTTGATTGA TCGTAACCAG ATGAAGCACT CTTTCCACTA TCCCTACAGT GTTATGGCTT GAACAATCAC GAAACAATAA TTGGTACGTA CGATCTTTCA GCCGACTCAA ACATCAAATC TTACAAATGT AGTCTTTGAA AGTATTACAT ATGTAAGATT TAAATGCAAC CGTTTTTTCG GAAGGAAATG ATGACCTCGT TTCCACCGGA ATTAGCTTGG TACCAAAGGA GGTAAGGATC ACTAGAAAAT TTTTTAAAAA ATCTCTTGAC ATTGGAAGGG AGATATGTTA TTATAAGAAT TGCGGAATTG TGAGCGGATA ACAATTCCCA TATAAAGGAG GAAGGATCAA TGATTCAAAA ACGAAAGCGG ACAGTTTCGT TCAGACTTGT GCTTATGTGC ACGCTGTTAT TTGTCAGTTT GCCGATTACA AAAGCATCAG CTGCTGGATC CATGTCTAGA GTCGACGTCG CTCATCACCA TCACCATCAC CATCACTAAC GTCCCCGGGG CAGCCCGCCT AATGAGCGGG CTTTTTTCAC GTCACGCGTC CATGGAGATC TTTGTCTGCA ACTGAAAAGT TTATACCTTA CCTGGAACAA ATGGTTGAAA CATACGAGGC TAATATCGGC TTATTAGGAA TAGTCCCTGT ACTAATAAAA TCAGGTGGAT CAGTTGATCA GTATATTTTG GACGAAGCTC GGAAAGAATT TGGAGATGAC TTGCTTAATT CCACAATTAA ATTAAGGGAA AGAATAAAGC GATTTGATGT TCAAGGAATC ACGGAAGAAG ATACTCATGA TAAAGAAGCT CTAAAACTAT TCAATAACCT TACAATGGAA TTGATCGAAA GGGTGGAAGG TTAATGGTAC GAAAATTAGG GGATCTACCT AGAAAGCCAC AAGGCGATAG GTCAAGCTTA AAGAACCCTT ACATGGATCT TACAGATTCT GAAAGTAAAG AAACAACAGA GGTTAAACAA ACAGAACCAA AAAGAAAAAA AGCATTGTTG AAAACAATGA AAGTTGATGT TTCAATCCAT AATAAGATTA AATCGCTGCA CGAAATTCTG GCAGCATCCG AAGGGAATTC ATATTACTTA GAGGATACTA TTGAGAGAGC TATTGATAAG ATGGTTGAGA CATTACCTGA GAGCCAAAAA ACTTTTTATG AATATGAATT AAAAAAAAGA ACCAACAAAG GCTGAGACAG ACTCCAAACG AGTCTGTTTT TTTAAAAAAA ATATTAGGAG CATTGAATAT ATATTAGAGA ATTAAGAAAG ACATGGGAAT AAAAATATTT TAAATCCAGT AAAAATATGA TAAGATTATT TCAGAATATG AAGAACTCTG TTTGTTTTTG ATGAAAAAAC AAACAAAAAA AATCCACCTA ACGGAATCTC AATTTAACTA ACAGCGGCCA AACTGAGAAG TTAAATTTGA GAAGGGGAAA AGGCGGATTT ATACTTGTAT TTAACTATCT CCATTTTAAC ATTTTATTAA ACCCCATACA AGTGAAAATC CTCTTTTACA CTGTTCCTTT AGGTGATCGC GGAGGGACAT TATGAGTGAA GTAAACCTAA AAGGAAATAC AGATGAATTA GTGTATTATC GACAGCAAAC CACTGGAAAT AAAATCGCCA GGAAGAGAAT CAAAAAAGGG AAAGAAGAAG TTTATTATGT TGCTGAAACG GAAGAGAAGA TATGGACAGA AGAGCAAATA AAAAACTTTT CTTTAGACAA ATTTGGTACG CATATACCTT ACATAGAAGG TCATTATACA ATCTTAAATA ATTACTTCTT TGATTTTTGG GGCTATTTTT TAGGTGCTGA AGGAATTGCG CTCTATGCTC ACCTAACTCG TTATGCATAC GGCAGCAAAG ACTTTTGCTT TCCTAGTCTA CAAACAATCG CTAAAAAAAT GGACAAGACT CCTGTTACAG TTAGAGGCTA CTTGAAACTG CTTGAAAGGT ACGGTTTTAT TTGGAAGGTA AACGTCCGTA ATAAAACCAA GGATAACACA GAGGAATCCC CGATTTTTAA GATTAGACGT AAGGTTCCTT TGCTTTCAGA AGAACTTTTA AATGGAAACC CTAATATTGA AATTCCAGAT GACGAGGAAG CACATGTAAA GAAGGCTTTA AAAAAGGAAA AAGAGGGTCT TCCAAAGGTT TTGAAAAAAG AGCACGATGA ATTTGTTAAA AAAATGATGG ATGAGTCAGA AACAATTAAT ATTCCAGAGG CCTTACAATA TGACACAATG TATGAAGATA TACTCAGTAA AGGAGAAATT CGAAAAGAAA TCAAAAAACA AATACCTAAT CCTACAACAT CTTTTGAGAG TATATCAATG ACAACTGAAG AGGAAAAAGT CGACAGTACT TTAAAAAGCG AAATGCAAAA TCGTGTCTCT AAGCCTTCTT TTGATACCTG GTTTAAAAAC ACTAAGATCA AAATTGAAAA TAAAAATTGT TTATTACTTG TACCGAGTGA ATTTGCATTT GAATGGATTA AGAAAAGATA TTTAGAAACA ATTAAAACAG TCCTTGAAGA AGCTGGATAT GTTTTCGAAA AAATCGAACT AAGAAAAGTG CAATAAACTG CTGAAGTATT TCAGCAGTTT TTTTTATTTA GAAATAGTGA AAAAAATATA ATCAGGGAGG TATCAATATT TAATGAGTAC TGATTTAAAT TTATTTAGAC TGGAATTAAT AATTAACACG TAGACTAATT AAAATTTAAT GAGGGATAAA GAGGATACAA AAATATTAAT TTCAATCCCT ATTAAATTTT AACAAGGGGG GGATTAAAAT TTAATTAGAG GTTTATCCAC AAGAAAAGAC CCTAATAAAA TTTTTACTAG GGTTATAACA CTGATTAATT TCTTAATGGG GGAGGGATTA AAATTTAATG ACAAAGAAAA CAATCTTTTA AGAAAAGCTT TTAAAAGATA ATAATAAAAA GAGCTTTGCG ATTAAGCAAA ACTCTTTACT TTTTCATTGA CATTATCAAA TTCATCGATT TCAAATTGTT GTTGTATCAT AAAGTTAATT CTGTTTTGCA CAACCTTTTC AGGAATATAA AACACATCTG AGGCTTGTTT TATAAACTCA GGGTCGCTAA AGTCAATGTA ACGTAGCATA TGATATGGTA TAGCTTCCAC CCAAGTTAGC CTTTCTGCTT CTTCTGAATG TTTTTCATAT ACTTCCATGG GTATCTCTAA ATGATTTTCC TCATGTAGCA AGGTATGAGC AAAAAGTTTA TGGAATTGAT AGTTCCTCTC TTTTTCTTCA ACTTTTTTAT CTAAAACAAA CACTTTAACA TCTGAGTCAA TGTAAGCATA AGATGTTTTT CCAGTCATAA TTTCAATCCC AAATCTTTTA GACAGAAATT CTGGACGTAA ATCTTTTGGT GAAAGAATTT TTTTATGTAG CAATATATCC GATACAGCAC CTTCTAAAAG CGTTGGTGAA TAGGGCATTT TACCTATCTC CTCTCATTTT GTGGAATAAA AATAGTCATA TTCGTCCATC TACCTATCCT ATTATCGAAC AGTTGAACTT TTTAATCAAG GATCAGTCCT TTTTTTCATT ATTCTTAAAC TGTGCTCTTA ACTTTAACAA CTCGATTTGT TTTTCCAGAT CTCGAGGGTA ACTAGCCTCG CCGATCCCGC AAGAGGCCCG GCAGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACG
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