pCS2-AHR
$99.00
In stock
SKU
PL73718
NM_001621.5;bHLHe76;RP85
100μg liquid plasmid(Instantly):$120
| name | pCS2-AHR | another name | apCS2-AHR |
| Insert Size (bp) | 2547bp | Plasmid host | Mammary cell,Fish |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCS2+ | 5′ sequencing primer | CMV-F： |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
CAACTGTTGG GAAGGGCGAT CGGTGCGGGC CTCTTCGCTA TTACGCCAGT CGACCATAGC CAATTCAATA TGGCGTATAT GGACTCATGC CAATTCAATA TGGTGGATCT GGACCTGTGC CAATTCAATA TGGCGTATAT GGACTCGTGC CAATTCAATA TGGTGGATCT GGACCCCAGC CAATTCAATA TGGCGGACTT GGCACCATGC CAATTCAATA TGGCGGACTT GGCACTGTGC CAACTGGGGA GGGGTCTACT TGGCACGGTG CCAAGTTTGA GGAGGGGTCT TGGCCCTGTG CCAAGTCCGC CATATTGAAT TGGCATGGTG CCAATAATGG CGGCCATATT GGCTATATGC CAGGATCAAT ATATAGGCAA TATCCAATAT GGCCCTATGC CAATATGGCT ATTGGCCAGG TTCAATACTA TGTATTGGCC CTATGCCATA TAGTATTCCA TATATGGGTT TTCCTATTGA CGTAGATAGC CCCTCCCAAT GGGCGGTCCC ATATACCATA TATGGGGCTT CCTAATACCG CCCATAGCCA CTCCCCCATT GACGTCAATG GTCTCTATAT ATGGTCTTTC CTATTGACGT CATATGGGCG GTCCTATTGA CGTATATGGC GCCTCCCCCA TTGACGTCAA TTACGGTAAA TGGCCCGCCT GGCTCAATGC CCATTGACGT CAATAGGACC ACCCACCATT GACGTCAATG GGATGGCTCA TTGCCCATTC ATATCCGTTC TCACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCAC TTGGCAGTAC ATCAATATCT ATTAATAGTA ACTTGGCAAG TACATTACTA TTGGAAGGAC GCCAGGGTAC ATTGGCAGTA CTCCCATTGA CGTCAATGGC GGTAAATGGC CCGCGATGGC TGCCAAGTAC ATCCCCATTG ACGTCAATGG GGAGGGGCAA TGACGCAAAT GGGCGTTCCA TTGACGTAAA TGGGCGGTAG GCGTGCCTAA TGGGAGGTCT ATATAAGCAA TGCTCGTTTA GGGAACCGCC ATTCTGCCTG GGGACGTCGG AGCAAGCTTG ATTTAGGTGA CACTATAGAA TACAAGCTAC TTGTTCTTTT TGCAGGATCC GCCACCATGA ACAGCAGCAG CGCCAACATC ACCTACGCCA GTCGCAAGCG GCGGAAGCCG GTGCAGAAAA CAGTAAAGCC AATCCCAGCT GAAGGAATCA AGTCAAATCC TTCCAAGCGG CATAGAGACC GACTTAATAC AGAGTTGGAC CGTTTGGCTA GCCTGCTGCC TTTCCCACAA GATGTTATTA ATAAGTTGGA CAAACTTTCA GTTCTTAGGC TCAGCGTCAG TTACCTGAGA GCCAAGAGCT TCTTTGATGT TGCATTAAAA TCCTCCCCTA CTGAAAGAAA CGGAGGCCAG GATAACTGTA GAGCAGCAAA TTTCAGAGAA GGCCTGAACT TACAAGAAGG AGAATTCTTA TTACAGGCTC TGAATGGCTT TGTATTAGTT GTCACTACAG ATGCTTTGGT CTTTTATGCT TCTTCTACTA TACAAGATTA TCTAGGGTTT CAGCAGTCTG ATGTCATACA TCAGAGTGTA TATGAACTTA TCCATACCGA AGACCGAGCT GAATTTCAGC GTCAGCTACA CTGGGCATTA AATCCTTCTC AGTGTACAGA GTCTGGACAA GGAATTGAAG AAGCCACTGG TCTCCCCCAG ACAGTAGTCT GTTATAACCC AGACCAGATT CCTCCAGAAA ACTCTCCTTT AATGGAGAGG TGCTTCATAT GTCGTCTAAG GTGTCTGCTG GATAATTCAT CTGGTTTTCT GGCAATGAAT TTCCAAGGGA AGTTAAAGTA TCTTCATGGA CAGAAAAAGA AAGGGAAAGA TGGATCAATA CTTCCACCTC AGTTGGCTTT GTTTGCGATA GCTACTCCAC TTCAGCCACC ATCCATACTT GAAATCCGGA CCAAAAATTT TATCTTTAGA ACCAAACACA AACTAGACTT CACACCTATT GGTTGTGATG CCAAAGGAAG AATTGTTTTA GGATATACTG AAGCAGAGCT GTGCACGAGA GGCTCAGGTT ATCAGTTTAT TCATGCAGCT GATATGCTTT ATTGTGCCGA GTCCCATATC CGAATGATTA AGACTGGAGA AAGTGGCATG ATAGTTTTCC GGCTTCTTAC AAAAAACAAC CGATGGACTT GGGTCCAGTC TAATGCACGC CTGCTTTATA AAAATGGAAG ACCAGATTAT ATCATTGTAA CTCAGAGACC ACTAACAGAT GAGGAAGGAA CAGAGCATTT ACGAAAACGA AATACGAAGT TGCCTTTTAT GTTTACCACT GGAGAAGCTG TGTTGTATGA GGCAACCAAC CCTTTTCCTG CCATAATGGA TCCCTTACCA CTAAGGACTA AAAATGGCAC TAGTGGAAAA GACTCTGCTA CCACATCCAC TCTAAGCAAG GACTCTCTCA ATCCTAGTTC CCTCCTGGCT GCCATGATGC AACAAGATGA GTCTATTTAT CTCTATCCTG CTTCAAGTAC TTCAAGTACT GCACCTTTTG AAAACAACTT TTTCAACGAA TCTATGAATG AATGCAGAAA TTGGCAAGAT AATACTGCAC CGATGGGAAA TGATACTATC CTGAAACATG AGCAAATTGA CCAGCCTCAG GATGTGAACT CATTTGCTGG AGGTCACCCA GGGCTCTTTC AAGATAGTAA AAACAGTGAC TTGTACAGCA TAATGAAAAA CCTAGGCATT GATTTTGAAG ACATCAGACA CATGCAGAAT GAAAAATTTT TCAGAAATGA TTTTTCTGGT GAGGTTGACT TCAGAGACAT TGACTTAACG GATGAAATCC TGACGTATGT CCAAGATTCT TTAAGTAAGT CTCCCTTCAT ACCTTCAGAT TATCAACAGC AACAGTCCTT GGCTCTGAAC TCAAGCTGTA TGGTACAGGA ACACCTACAT CTAGAACAGC AACAGCAACA TCACCAAAAG CAAGTAGTAG TGGAGCCACA GCAACAGCTG TGTCAGAAGA TGAAGCACAT GCAAGTTAAT GGCATGTTTG AAAATTGGAA CTCTAACCAA TTCGTGCCTT TCAATTGTCC ACAGCAAGAC CCACAACAAT ATAATGTCTT TACAGACTTA CATGGGATCA GTCAAGAGTT CCCCTACAAA TCTGAAATGG ATTCTATGCC TTATACACAG AACTTTATTT CCTGTAATCA GCCTGTATTA CCACAACATT CCAAATGTAC AGAGCTGGAC TACCCTATGG GGAGTTTTGA ACCATCCCCA TACCCCACTA CTTCTAGTTT AGAAGATTTT GTCACTTGTT TACAACTTCC TGAAAACCAA AAGCATGGAT TAAATCCACA GTCAGCCATA ATAACTCCTC AGACATGTTA TGCTGGGGCC GTGTCGATGT ATCAGTGCCA GCCAGAACCT CAGCACACCC ACGTGGGTCA GATGCAGTAC AATCCAGTAC TGCCAGGCCA ACAGGCATTT TTAAACAAGT TTCAGAATGG AGTTTTAAAT GAAACATATC CAGCTGAATT AAATAACATA AATAACACTC AGACTACCAC ACATCTTCAG CCACTTCATC ATCCGTCAGA AGCCAGACCT TTTCCTGATT TGACATCCAG TGGATTCCTG TAACTCGAGC CTCTAGAACT ATAGTGAGTC GTATTACGTA GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAATT CGCGGCCGCG GCGCCAATGC ATTGGGCCCG GTACCCAGCT TTTGTTCCCT TTAGTGAGGG TTAATTGCGC GCTTGGCGTA ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTAA ATTGTAAGCG TTAATATTTT GTTAAAATTC GCGTTAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAAAATC CCTTATAAAT CAAAAGAATA GACCGAGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC GATGGCCCAC TACGTGAACC ATCACCCTAA TCAAGTTTTT TGGGGTCGAG GTGCCGTAAA GCACTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCCGCGC TTAATGCGCC GCTACAGGGC GCGTCCCATT CGCCATTCAG GCTGCG
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