pCDNA3.1-SCN2A-3×FLAG
$142.00
In stock
SKU
PL73595
NM_021007.3;NM_001371247.1;BFIC3;BFIS3;BFNIS
100μg liquid plasmid(Instantly):$178
| name | pCDNA3.1-SCN2A-3×FLAG | another name | apCDNA3.1-SCN2A-3×FLAG |
| Insert Size (bp) | 6015bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | pCDNA3.1 | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG |
| 3′ sequencing primer | BGH：TAGAAGGCACAGTCGAGG | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGT TAAGCTTGGT ACCGAGCTCG GATCCGCCAC CATGGCACAG TCAGTGCTGG TACCGCCAGG ACCTGACAGC TTCCGCTTCT TTACCAGGGA ATCCCTTGCT GCTATTGAAC AACGCATTGC AGAAGAGAAA GCTAAGAGAC CCAAACAGGA ACGCAAGGAT GAGGATGATG AAAATGGCCC AAAGCCAAAC AGTGACTTGG AAGCAGGAAA ATCTCTTCCA TTTATTTATG GAGACATTCC TCCAGAGATG GTGTCAGTGC CCCTGGAGGA TCTGGACCCC TACTATATCA ATAAGAAAAC GTTTATAGTA TTGAATAAAG GGAAAGCAAT CTCTCGATTC AGTGCCACCC CTGCCCTTTA CATTTTAACT CCCTTCAACC CTATTAGAAA ATTAGCTATT AAGATTTTGG TACATTCTTT ATTCAATATG CTCATTATGT GCACGATTCT TACCAACTGT GTATTTATGA CCATGAGTAA CCCTCCAGAC TGGACAAAGA ATGTGGAGTA TACCTTTACA GGAATTTATA CTTTTGAATC ACTTATTAAA ATACTTGCAA GGGGCTTTTG TTTAGAAGAT TTCACATTTT TACGGGATCC ATGGAATTGG TTGGATTTCA CAGTCATTAC TTTTGCATAT GTGACAGAGT TTGTGGACCT GGGCAATGTC TCAGCGTTGA GAACATTCAG AGTTCTCCGA GCATTGAAAA CAATTTCAGT CATTCCAGGC CTGAAGACCA TTGTGGGGGC CCTGATCCAG TCAGTGAAGA AGCTTTCTGA TGTCATGATC TTGACTGTGT TCTGTCTAAG CGTGTTTGCG CTAATAGGAT TGCAGTTGTT CATGGGCAAC CTACGAAATA AATGTTTGCA ATGGCCTCCA GATAATTCTT CCTTTGAAAT AAATATCACT TCCTTCTTTA ACAATTCATT GGATGGGAAT GGTACTACTT TCAATAGGAC AGTGAGCATA TTTAACTGGG ATGAATATAT TGAGGATAAA AGTCACTTTT ATTTTTTAGA GGGGCAAAAT GATGCTCTGC TTTGTGGCAA CAGCTCAGAT GCAGGCCAGT GTCCTGAAGG ATACATCTGT GTGAAGGCTG GTAGAAACCC CAACTATGGC TACACGAGCT TTGACACCTT TAGTTGGGCC TTTTTGTCCT TATTTCGTCT CATGACTCAA GACTTCTGGG AAAACCTTTA TCAACTGACA CTACGTGCTG CTGGGAAAAC GTACATGATA TTTTTTGTGC TGGTCATTTT CTTGGGCTCA TTCTATCTAA TAAATTTGAT CTTGGCTGTG GTGGCCATGG CCTATGAGGA ACAGAATCAG GCCACATTGG AAGAGGCTGA ACAGAAGGAA GCTGAATTTC AGCAGATGCT CGAACAGTTG AAAAAGCAAC AAGAAGAAGC TCAGGCGGCA GCTGCAGCCG CATCTGCTGA ATCAAGAGAC TTCAGTGGTG CTGGTGGGAT AGGAGTTTTT TCAGAGAGTT CTTCAGTAGC ATCTAAGTTG AGCTCCAAAA GTGAAAAAGA GCTGAAAAAC AGAAGAAAGA AAAAGAAACA GAAAGAACAG TCTGGAGAAG AAGAGAAAAA TGACAGAGTC CGAAAATCGG AATCTGAAGA CAGCATAAGA AGAAAAGGTT TCCGTTTTTC CTTGGAAGGA AGTAGGCTGA CATATGAAAA GAGATTTTCT TCTCCACACC AGTCCTTACT GAGCATCCGT GGCTCCCTTT TCTCTCCAAG ACGCAACAGT AGGGCGAGCC TTTTCAGCTT CAGAGGTCGA GCAAAGGACA TTGGCTCTGA GAATGACTTT GCTGATGATG AGCACAGCAC CTTTGAGGAC AATGACAGCC GAAGAGACTC TCTGTTCGTG CCGCACAGAC ATGGAGAACG GCGCCACAGC AATGTCAGCC AGGCCAGCCG TGCCTCCAGG GTGCTCCCCA TCCTGCCCAT GAATGGGAAG ATGCATAGCG CTGTGGACTG CAATGGTGTG GTCTCCCTGG TCGGGGGCCC TTCTACCCTC ACATCTGCTG GGCAGCTCCT ACCAGAGGGC ACAACTACTG AAACAGAAAT AAGAAAGAGA CGGTCCAGTT CTTATCATGT TTCCATGGAT TTATTGGAAG ATCCTACATC AAGGCAAAGA GCAATGAGTA TAGCCAGTAT TTTGACCAAC ACCATGGAAG AACTTGAAGA ATCCAGACAG AAATGCCCAC CATGCTGGTA TAAATTTGCT AATATGTGTT TGATTTGGGA CTGTTGTAAA CCATGGTTAA AGGTGAAACA CCTTGTCAAC CTGGTTGTAA TGGACCCATT TGTTGACCTG GCCATCACCA TCTGCATTGT CTTAAATACA CTCTTCATGG CTATGGAGCA CTATCCCATG ACGGAGCAGT TCAGCAGTGT ACTGTCTGTT GGAAACCTGG TCTTCACAGG GATCTTCACA GCAGAAATGT TTCTCAAGAT AATTGCCATG GATCCATATT ATTACTTTCA AGAAGGCTGG AATATTTTTG ATGGTTTTAT TGTGAGCCTT AGTTTAATGG AACTTGGTTT GGCAAATGTG GAAGGATTGT CAGTTCTCCG ATCATTCCGG CTGCTCCGAG TTTTCAAGTT GGCAAAATCT TGGCCAACTC TAAATATGCT AATTAAGATC ATTGGCAATT CTGTGGGGGC TCTAGGAAAC CTCACCTTGG TATTGGCCAT CATCGTCTTC ATTTTTGCTG TGGTCGGCAT GCAGCTCTTT GGTAAGAGCT ACAAAGAATG TGTCTGCAAG ATTTCCAATG ATTGTGAACT CCCACGCTGG CACATGCATG ACTTTTTCCA CTCCTTCCTG ATCGTGTTCC GCGTGCTGTG TGGAGAGTGG ATAGAGACCA TGTGGGACTG TATGGAGGTC GCTGGCCAAA CCATGTGCCT TACTGTCTTC ATGATGGTCA TGGTGATTGG AAATCTAGTG GTTCTGAACC TCTTCTTGGC CTTGCTTTTG AGTTCCTTCA GTTCTGACAA TCTTGCTGCC ACTGATGATG ATAACGAAAT GAATAATCTC CAGATTGCTG TGGGAAGGAT GCAGAAAGGA ATCGATTTTG TTAAAAGAAA AATACGTGAA TTTATTCAGA AAGCCTTTGT TAGGAAGCAG AAAGCTTTAG ATGAAATTAA ACCGCTTGAA GATCTAAATA ATAAAAAAGA CAGCTGTATT TCCAACCATA CCACCATAGA AATAGGCAAA GACCTCAATT ATCTCAAAGA CGGAAATGGA ACTACTAGTG GCATAGGCAG CAGTGTAGAA AAATATGTCG TGGATGAAAG TGATTACATG TCATTTATAA ACAACCCTAG CCTCACTGTG ACAGTACCAA TTGCTGTTGG AGAATCTGAC TTTGAAAATT TAAATACTGA AGAATTCAGC AGCGAGTCAG ATATGGAGGA AAGCAAAGAG AAGCTAAATG CAACTAGTTC ATCTGAAGGC AGCACGGTTG ATATTGGAGC TCCCGCCGAG GGAGAACAGC CTGAGGTTGA ACCTGAGGAA TCCCTTGAAC CTGAAGCCTG TTTTACAGAA GACTGTGTAC GGAAGTTCAA GTGTTGTCAG ATAAGCATAG AAGAAGGCAA AGGGAAACTC TGGTGGAATT TGAGGAAAAC ATGCTATAAG ATAGTGGAGC ACAATTGGTT CGAAACCTTC ATTGTCTTCA TGATTCTGCT GAGCAGTGGG GCTCTGGCCT TTGAAGATAT ATACATTGAG CAGCGAAAAA CCATTAAGAC CATGTTAGAA TATGCTGACA AGGTTTTCAC TTACATATTC ATTCTGGAAA TGCTGCTAAA GTGGGTTGCA TATGGTTTTC AAGTGTATTT TACCAATGCC TGGTGCTGGC TAGACTTCCT GATTGTTGAT GTCTCACTGG TTAGCTTAAC TGCAAATGCC TTGGGTTACT CAGAACTTGG TGCCATCAAA TCCCTCAGAA CACTAAGAGC TCTGAGGCCA CTGAGAGCTT TGTCCCGGTT TGAAGGAATG AGGGTTGTTG TAAATGCTCT TTTAGGAGCC ATTCCATCTA TCATGAATGT ACTTCTGGTT TGTCTGATCT TTTGGCTAAT ATTCAGTATC ATGGGAGTGA ATCTCTTTGC TGGCAAGTTT TACCATTGTA TTAATTACAC CACTGGAGAG ATGTTTGATG TAAGCGTGGT CAACAACTAC AGTGAGTGCA AAGCTCTCAT TGAGAGCAAT CAAACTGCCA GGTGGAAAAA TGTGAAAGTA AACTTTGATA ACGTAGGACT TGGATATCTG TCTCTACTTC AAGTAGCCAC GTTTAAGGGA TGGATGGATA TTATGTATGC AGCTGTTGAT TCACGAAATG TAGAATTACA ACCCAAGTAT GAAGACAACC TGTACATGTA TCTTTATTTT GTCATCTTTA TTATTTTTGG TTCATTCTTT ACCTTGAATC TTTTCATTGG TGTCATCATA GATAACTTCA ACCAACAGAA AAAGAAGTTT GGAGGTCAAG ACATTTTTAT GACAGAAGAA CAGAAGAAAT ACTACAATGC AATGAAAAAA CTGGGTTCAA AGAAACCACA AAAACCCATA CCTCGACCTG CTAACAAATT CCAAGGAATG GTCTTTGATT TTGTAACCAA ACAAGTCTTT GATATCAGCA TCATGATCCT CATCTGCCTT AACATGGTCA CCATGATGGT GGAAACCGAT GACCAGAGTC AAGAAATGAC AAACATTCTG TACTGGATTA ATCTGGTGTT TATTGTTCTG TTCACTGGAG AATGTGTGCT GAAACTGATC TCTCTTCGTT ACTACTATTT CACTATTGGA TGGAATATTT TTGATTTTGT GGTGGTCATT CTCTCCATTG TAGGAATGTT TCTGGCTGAA CTGATAGAAA AGTATTTTGT GTCCCCTACC CTGTTCCGAG TGATCCGTCT TGCCAGGATT GGCCGAATCC TACGTCTGAT CAAAGGAGCA AAGGGGATCC GCACGCTGCT CTTTGCTTTG ATGATGTCCC TTCCTGCGTT GTTTAACATC GGCCTCCTTC TTTTCCTGGT CATGTTCATC TACGCCATCT TTGGGATGTC CAATTTTGCC TATGTTAAGA GGGAAGTTGG GATCGATGAC ATGTTCAACT TTGAGACCTT TGGCAACAGC ATGATCTGCC TGTTCCAAAT TACAACCTCT GCTGGCTGGG ATGGATTGCT AGCACCTATT CTTAATAGTG GACCTCCAGA CTGTGACCCT GACAAAGATC ACCCTGGAAG CTCAGTTAAA GGAGACTGTG GGAACCCATC TGTTGGGATT TTCTTTTTTG TCAGTTACAT CATCATATCC TTCCTGGTTG TGGTGAACAT GTACATCGCG GTCATCCTGG AGAACTTCAG TGTTGCTACT GAAGAAAGTG CAGAGCCTCT GAGTGAGGAT GACTTTGAGA TGTTCTATGA GGTTTGGGAG AAGTTTGATC CCGATGCGAC CCAGTTTATA GAGTTTGCCA AACTTTCTGA TTTTGCAGAT GCCCTGGATC CTCCTCTTCT CATAGCAAAA CCCAACAAAG TCCAGCTCAT TGCCATGGAT CTGCCCATGG TGAGTGGTGA CCGGATCCAC TGTCTTGACA TCTTATTTGC TTTTACAAAG CGTGTTTTGG GTGAGAGTGG AGAGATGGAT GCCCTTCGAA TACAGATGGA AGAGCGATTC ATGGCATCAA ACCCCTCCAA AGTCTCTTAT GAGCCCATTA CGACCACGTT GAAACGCAAA CAAGAGGAGG TGTCTGCTAT TATTATCCAG AGGGCTTACA GACGCTACCT CTTGAAGCAA AAAGTTAAAA AGGTATCAAG TATATACAAG AAAGACAAAG GCAAAGAATG TGATGGAACA CCCATCAAAG AAGATACTCT CATTGATAAA CTGAATGAGA ATTCAACTCC AGAGAAAACC GATATGACGC CTTCCACCAC GTCTCCACCC TCGTATGATA GTGTGACCAA ACCAGAAAAA GAAAAATTTG AAAAAGACAA ATCAGAAAAG GAAGACAAAG GGAAAGATAT CAGGGAAAGT AAAAAGCTCG AGTCTAGAGG GCCCTTCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC AAGTGAGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACAAGC ATCACGAAAT CTGACGCTCA AATCAGTGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGCGGC TCCCTCGTGC GCTCTCCTGT TCCTGCCTTT CGGTTTACCG GTGTCATTCC GCTGTTATGG CCGCGTTTGT CTCATTCCAC GCCTGACACT CAGTTCCGGG TAGGCAGTTC GCTCCAAGCT GGACTGTATG CACGAACCCC CCGTTCAGTC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGAAA GACATGCAAA AGCACCACTG GCAGCAGCCA CTGGTAATTG ATTTAGAGGA GTTAGTCTTG AAGTCATGCG CCGGTTAAGG CTAAACTGAA AGGACAAGTT TTGGTGACTG CGCTCCTCCA AGCCAGTTAC CTCGGTTCAA AGAGTTGGTA GCTCAGAGAA CCTTCGAAAA ACCGCCCTGC AAGGCGGTTT TTTCGTTTTC AGAGCAAGAG ATTACGCGCA GACCAAAACG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTC
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