pX458-dCas9-DNMT3A
$113.00
In stock
SKU
PL72903
100μg liquid plasmid(Instantly):$134
| name | pX458-dCas9-DNMT3A | another name | apX458-dCas9-DNMT3A |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | U6 | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGGCCTAT TTCCCATGAT TCCTTCATAT TTGCATATAC GATACAAGGC TGTTAGAGAG ATAATTGGAA TTAATTTGAC TGTAAACACA AAGATATTAG TACAAAATAC GTGACGTAGA AAGTAATAAT TTCTTGGGTA GTTTGCAGTT TTAAAATTAT GTTTTAAAAT GGACTATCAT ATGCTTACCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAACACCG GGTCTTCGAG AAGACCTGTT TTAGAGCTAG AAATAGCAAG TTAAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG TGCTTTTTTG TTTTAGAGCT AGAAATAGCA AGTTAAAATA AGGCTAGTCC GTTTTTAGCG CGTGCGCCAA TTCTGCAGAC AAATGGCTCT AGAGGTACCC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAG TAACGCCAAT AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT TGTGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG GCGGGGGGGG GGGGGGGGCG CGCGCCAGGC GGGGCGGGGC GGGGCGAGGG GCGGGGCGGG GCGAGGCGGA GAGGTGCGGC GGCAGCCAAT CAGAGCGGCG CGCTCCGAAA GTTTCCTTTT ATGGCGAGGC GGCGGCGGCG GCGGCCCTAT AAAAAGCGAA GCGCGCGGCG GGCGGGAGTC GCTGCGACGC TGCCTTCGCC CCGTGCCCCG CTCCGCCGCC GCCTCGCGCC GCCCGCCCCG GCTCTGACTG ACCGCGTTAC TCCCACAGGT GAGCGGGCGG GACGGCCCTT CTCCTCCGGG CTGTAATTAG CTGAGCAAGA GGTAAGGGTT TAAGGGATGG TTGGTTGGTG GGGTATTAAT GTTTAATTAC CTGGAGCACC TGCCTGAAAT CACTTTTTTT CAGGTTGGAC CGGTGCCACC ATGGACAAGA AGTATTCTAT CGGACTGGCC ATCGGGACTA ATAGCGTCGG GTGGGCCGTG ATCACTGACG AGTACAAGGT GCCCTCTAAG AAGTTCAAGG TGCTCGGGAA CACCGACCGG CATTCCATCA AGAAAAATCT GATCGGAGCT CTCCTCTTTG ATTCAGGGGA GACCGCTGAA GCAACCCGCC TCAAGCGGAC TGCTAGACGG CGGTACACCA GGAGGAAGAA CCGGATTTGT TACCTTCAAG AGATATTCTC CAACGAAATG GCAAAGGTCG ACGACAGCTT CTTCCATAGG CTGGAAGAAT CATTCCTCGT GGAAGAGGAT AAGAAGCATG AACGGCATCC CATCTTCGGT AATATCGTCG ACGAGGTGGC CTATCACGAG AAATACCCAA CCATCTACCA TCTTCGCAAA AAGCTGGTGG ACTCAACCGA CAAGGCAGAC CTCCGGCTTA TCTACCTGGC CCTGGCCCAC ATGATCAAGT TCAGAGGCCA CTTCCTGATC GAGGGCGACC TCAATCCTGA CAATAGCGAT GTGGATAAAC TGTTCATCCA GCTGGTGCAG ACTTACAACC AGCTCTTTGA AGAGAACCCC ATCAATGCAA GCGGAGTCGA TGCCAAGGCC ATTCTGTCAG CCCGGCTGTC AAAGAGCCGC AGACTTGAGA ATCTTATCGC TCAGCTGCCG GGTGAAAAGA AAAATGGACT GTTCGGGAAC CTGATTGCTC TTTCACTTGG GCTGACTCCC AATTTCAAGT CTAATTTCGA CCTGGCAGAG GATGCCAAGC TGCAACTGTC CAAGGACACC TATGATGACG ATCTCGACAA CCTCCTGGCC CAGATCGGTG ACCAATACGC CGACCTTTTC CTTGCTGCTA AGAATCTTTC TGACGCCATC CTGCTGTCTG ACATTCTCCG CGTGAACACT GAAATCACCA AGGCCCCTCT TTCAGCTTCA ATGATTAAGC GGTATGATGA GCACCACCAG GACCTGACCC TGCTTAAGGC ACTCGTCCGG CAGCAGCTTC CGGAGAAGTA CAAGGAAATC TTCTTTGACC AGTCAAAGAA TGGATACGCC GGCTACATCG ACGGAGGTGC CTCCCAAGAG GAATTTTATA AGTTTATCAA ACCTATCCTT GAGAAGATGG ACGGCACCGA AGAGCTCCTC GTGAAACTGA ATCGGGAGGA TCTGCTGCGG AAGCAGCGCA CTTTCGACAA TGGGAGCATT CCCCACCAGA TCCATCTTGG GGAGCTTCAC GCCATCCTTC GGCGCCAAGA GGACTTCTAC CCCTTTCTTA AGGACAACAG GGAGAAGATT GAGAAAATTC TCACTTTCCG CATCCCCTAC TACGTGGGAC CCCTCGCCAG AGGAAATAGC CGGTTTGCTT GGATGACCAG AAAGTCAGAA GAAACTATCA CTCCCTGGAA CTTCGAAGAG GTGGTGGACA AGGGAGCCAG CGCTCAGTCA TTCATCGAAC GGATGACTAA CTTCGATAAG AACCTCCCCA ATGAGAAGGT CCTGCCGAAA CATTCCCTGC TCTACGAGTA CTTTACCGTG TACAACGAGC TGACCAAGGT GAAATATGTC ACCGAAGGGA TGAGGAAGCC CGCATTCCTG TCAGGCGAAC AAAAGAAGGC AATTGTGGAC CTTCTGTTCA AGACCAATAG AAAGGTGACC GTGAAGCAGC TGAAGGAGGA CTATTTCAAG AAAATTGAAT GCTTCGACTC TGTGGAGATT AGCGGGGTCG AAGATCGGTT CAACGCAAGC CTGGGTACCT ACCATGATCT GCTTAAGATC ATCAAGGACA AGGATTTTCT GGACAATGAG GAGAACGAGG ACATCCTTGA GGACATTGTC CTGACTCTCA CTCTGTTCGA GGACCGGGAA ATGATCGAGG AGAGGCTTAA GACCTACGCC CATCTGTTCG ACGATAAAGT GATGAAGCAA CTTAAACGGA GAAGATATAC CGGATGGGGA CGCCTTAGCC GCAAACTCAT CAACGGAATC CGGGACAAAC AGAGCGGAAA GACCATTCTT GATTTCCTTA AGAGCGACGG ATTCGCTAAT CGCAACTTCA TGCAACTTAT CCATGATGAT TCCCTGACCT TTAAGGAGGA CATCCAGAAG GCCCAAGTGT CTGGACAAGG TGACTCACTG CACGAGCATA TCGCAAATCT GGCTGGTTCA CCCGCTATTA AGAAGGGTAT TCTCCAGACC GTGAAAGTCG TGGACGAGCT GGTCAAGGTG ATGGGTCGCC ATAAACCAGA GAACATTGTC ATCGAGATGG CCAGGGAAAA CCAGACTACC CAGAAGGGAC AGAAGAACAG CAGGGAGCGG ATGAAAAGAA TTGAGGAAGG GATTAAGGAG CTCGGGTCAC AGATCCTTAA AGAGCACCCG GTGGAAAACA CCCAGCTTCA GAATGAGAAG CTCTATCTGT ACTACCTTCA AAATGGACGC GATATGTATG TGGACCAAGA GCTTGATATC AACAGGCTCT CAGACTACGA CGTGGACGCC ATCGTCCCTC AGAGCTTCCT CAAAGACGAC TCAATTGACA ATAAGGTGCT GACTCGCTCA GACAAGAACC GGGGAAAGTC AGATAACGTG CCCTCAGAGG AAGTCGTGAA AAAGATGAAG AACTATTGGC GCCAGCTTCT GAACGCAAAG CTGATCACTC AGCGGAAGTT CGACAATCTC ACTAAGGCTG AGAGGGGCGG ACTGAGCGAA CTGGACAAAG CAGGATTCAT TAAACGGCAA CTTGTGGAGA CTCGGCAGAT TACTAAACAT GTCGCCCAAA TCCTTGACTC ACGCATGAAT ACCAAGTACG ACGAAAACGA CAAACTTATC CGCGAGGTGA AGGTGATTAC CCTGAAGTCC AAGCTGGTCA GCGATTTCAG AAAGGACTTT CAATTCTACA AAGTGCGGGA GATCAATAAC TATCATCATG CTCATGACGC ATATCTGAAT GCCGTGGTGG GAACCGCCCT GATCAAGAAG TACCCAAAGC TGGAAAGCGA GTTCGTGTAC GGAGACTACA AGGTCTACGA CGTGCGCAAG ATGATTGCCA AATCTGAGCA GGAGATCGGA AAGGCCACCG CAAAGTACTT CTTCTACAGC AACATCATGA ATTTCTTCAA GACCGAAATC ACCCTTGCAA ACGGTGAGAT CCGGAAGAGG CCGCTCATCG AGACTAATGG GGAGACTGGC GAAATCGTGT GGGACAAGGG CAGAGATTTC GCTACCGTGC GCAAAGTGCT TTCTATGCCT CAAGTGAACA TCGTGAAGAA AACCGAGGTG CAAACCGGAG GCTTTTCTAA GGAATCAATC CTCCCCAAGC GCAACTCCGA CAAGCTCATT GCAAGGAAGA AGGATTGGGA CCCTAAGAAG TACGGCGGAT TCGATTCACC AACTGTGGCT TATTCTGTCC TGGTCGTGGC TAAGGTGGAA AAAGGAAAGT CTAAGAAGCT CAAGAGCGTG AAGGAACTGC TGGGTATCAC CATTATGGAG CGCAGCTCCT TCGAGAAGAA CCCAATTGAC TTTCTCGAAG CCAAAGGTTA CAAGGAAGTC AAGAAGGACC TTATCATCAA GCTCCCAAAG TATAGCCTGT TCGAACTGGA GAATGGGCGG AAGCGGATGC TCGCCTCCGC TGGCGAACTT CAGAAGGGTA ATGAGCTGGC TCTCCCCTCC AAGTACGTGA ATTTCCTCTA CCTTGCAAGC CATTACGAGA AGCTGAAGGG GAGCCCCGAG GACAACGAGC AAAAGCAACT GTTTGTGGAG CAGCATAAGC ATTATCTGGA CGAGATCATT GAGCAGATTT CCGAGTTTTC TAAACGCGTC ATTCTCGCTG ATGCCAACCT CGATAAAGTC CTTAGCGCAT ACAATAAGCA CAGAGACAAA CCAATTCGGG AGCAGGCTGA GAATATCATC CACCTGTTCA CCCTCACCAA TCTTGGTGCC CCTGCCGCAT TCAAGTACTT CGACACCACC ATCGACCGGA AACGCTATAC CTCCACCAAA GAAGTGCTGG ACGCCACCCT CATCCACCAG AGCATCACCG GACTTTACGA AACTCGGATT GACCTCTCAC AGCTCGGAGG GGATGAGGGA GCTGATCCAA AAAAGAAGAG AAAGGTAGAC CCAAAGAAAA AACGAAAAGT AGATCCGAAA AAGAAGAGGA AGGTGGGATC CATGCCCGCC ATGCCCTCCA GCGGCCCCGG GGACACCAGC AGCTCTGCTG CGGAGCGGGA GGAGGACCGA AAGGACGGAG AGGAGCAGGA GGAGCCGCGT GGCAAGGAGG AGCGCCAAGA GCCCAGCACC ACGGCACGGA AGGTGGGGCG GCCTGGGAGG AAGCGCAAGC ACCCCCCGGT GGAAAGCGGT GACACGCCAA AGGACCCTGC GGTGATCTCC AAGTCCCCAT CCATGGCCCA GGACTCAGGC GCCTCAGAGC TATTACCCAA TGGGGACTTG GAGAAGCGGA GTGAGCCCCA GCCAGAGGAG GGGAGCCCTG CTGGGGGGCA GAAGGGCGGG GCCCCAGCAG AGGGAGAGGG TGCAGCTGAG ACCCTGCCTG AAGCCTCAAG AGCAGTGGAA AATGGCTGCT GCACCCCCAA GGAGGGCCGA GGAGCCCCTG CAGAAGCGGG CAAAGAACAG AAGGAGACCA ACATCGAATC CATGAAAATG GAGGGCTCCC GGGGCCGGCT GCGGGGTGGC TTGGGCTGGG AGTCCAGCCT CCGTCAGCGG CCCATGCCGA GGCTCACCTT CCAGGCGGGG GACCCCTACT ACATCAGCAA GCGCAAGCGG GACGAGTGGC TGGCACGCTG GAAAAGGGAG GCTGAGAAGA AAGCCAAGGT CATTGCAGGA ATGAATGCTG TGGAAGAAAA CCAGGGGCCC GGGGAGTCTC AGAAGGTGGA GGAGGCCAGC CCTCCTGCTG TGCAGCAGCC CACTGACCCC GCATCCCCCA CTGTGGCTAC CACGCCTGAG CCCGTGGGGT CCGATGCTGG GGACAAGAAT GCCACCAAAG CAGGCGATGA CGAGCCAGAG TACGAGGACG GCCGGGGCTT TGGCATTGGG GAGCTGGTGT GGGGGAAACT GCGGGGCTTC TCCTGGTGGC CAGGCCGCAT TGTGTCTTGG TGGATGACGG GCCGGAGCCG AGCAGCTGAA GGCACCCGCT GGGTCATGTG GTTCGGAGAC GGCAAATTCT CAGTGGTGTG TGTTGAGAAG CTGATGCCGC TGAGCTCGTT TTGCAGTGCG TTCCACCAGG CCACGTACAA CAAGCAGCCC ATGTACCGCA AAGCCATCTA CGAGGTCCTG CAGGTGGCCA GCAGCCGCGC GGGGAAGCTG TTCCCGGTGT GCCACGACAG CGATGAGAGT GACACTGCCA AGGCCGTGGA GGTGCAGAAC AAGCCCATGA TTGAATGGGC CCTGGGGGGC TTCCAGCCTT CTGGCCCTAA GGGCCTGGAG CCACCAGAAG AAGAGAAGAA TCCCTACAAA GAAGTGTACA CGGACATGTG GGTGGAACCT GAGGCAGCTG CCTACGCACC ACCTCCACCA GCCAAAAAGC CCCGGAAGAG CACAGCGGAG AAGCCCAAGG TCAAGGAGAT TATTGATGAG CGCACAAGAG AGCGGCTGGT GTACGAGGTG CGGCAGAAGT GCCGGAACAT TGAGGACATC TGCATCTCCT GTGGGAGCCT CAATGTTACC CTGGAACACC CCCTCTTCGT TGGAGGAATG TGCCAAAACT GCAAGAACTG CTTTCTGGAG TGTGCGTACC AGTACGACGA CGACGGCTAC CAGTCCTACT GCACCATCTG CTGTGGGGGC CGTGAGGTGC TCATGTGCGG AAACAACAAC TGCTGCAGGT GCTTTTGCGT GGAGTGTGTG GACCTCTTGG TGGGGCCGGG GGCTGCCCAG GCAGCCATTA AGGAAGACCC CTGGAACTGC TACATGTGCG GGCACAAGGG TACCTACGGG CTGCTGCGGC GGCGAGAGGA CTGGCCCTCC CGGCTCCAGA TGTTCTTCGC TAATAACCAC GACCAGGAAT TTGACCCTCC AAAGGTTTAC CCACCTGTCC CAGCTGAGAA GAGGAAGCCC ATCCGGGTGC TGTCTCTCTT TGATGGAATC GCTACAGGGC TCCTGGTGCT GAAGGACTTG GGCATTCAGG TGGACCGCTA CATTGCCTCG GAGGTGTGTG AGGACTCCAT CACGGTGGGC ATGGTGCGGC ACCAGGGGAA GATCATGTAC GTCGGGGACG TCCGCAGCGT CACACAGAAG CATATCCAGG AGTGGGGCCC ATTCGATCTG GTGATTGGGG GCAGTCCCTG CAATGACCTC TCCATCGTCA ACCCTGCTCG CAAGGGCCTC TACGAGGGCA CTGGCCGGCT CTTCTTTGAG TTCTACCGCC TCCTGCATGA TGCGCGGCCC AAGGAGGGAG ATGATCGCCC CTTCTTCTGG CTCTTTGAGA ATGTGGTGGC CATGGGCGTT AGTGACAAGA GGGACATCTC GCGATTTCTC GAGTCCAACC CTGTGATGAT TGATGCCAAA GAAGTGTCAG CTGCACACAG GGCCCGCTAC TTCTGGGGTA ACCTTCCCGG TATGAACAGG CCGTTGGCAT CCACTGTGAA TGATAAGCTG GAGCTGCAGG AGTGTCTGGA GCATGGCAGG ATAGCCAAGT TCAGCAAAGT GAGGACCATT ACTACGAGGT CAAACTCCAT AAAGCAGGGC AAAGACCAGC ATTTTCCTGT CTTCATGAAT GAGAAAGAGG ACATCTTATG GTGCACTGAA ATGGAAAGGG TATTTGGTTT CCCAGTCCAC TATACTGACG TCTCCAACAT GAGCCGCTTG GCGAGGCAGA GACTGCTGGG CCGGTCATGG AGCGTGCCAG TCATCCGCCA CCTCTTCGCT CCGCTGAAGG AGTATTTTGC GTGTGTGGAA TTCGGCAGTG GAGAGGGCAG AGGAAGTCTG CTAACATGCG GTGACGTCGA GGAGAATCCT GGCCCAGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG GAATTCTAAC TAGAGCTCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA GAATAGCAGG CATGCTGGGG AGCGGCCGCA GGAACCCCTA GTGATGGAGT TGGCCACTCC CTCTCTGCGC GCTCGCTCGC TCACTGAGGC CGGGCGACCA AAGGTCGCCC GACGCCCGGG CTTTGCCCGG GCGGCCTCAG TGAGCGAGCG AGCGCGCAGC TGCCTGCAGG GGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGAAGCCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGT
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