pAtU6-26-sgRNA-35S-EGFP-Cas9
$128.00
In stock
SKU
PL72897
100μg liquid plasmid(Instantly):$149
| name | pAtU6-26-sgRNA-35S-EGFP-Cas9 | another name | apAtU6-26-sgRNA-35S-EGFP-Cas9 |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Gene editing | Fragment type | CRISPR |
| Fragment species | Empty vector | Prokaryotic resistance | Kan |
| Selective marker | Hyg | Fluorescent labeling | Green |
| Promoter | AtU6-26 | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTTACCCGC CAATATATCC TGTCAAACAC TGATAGTTTA AACTGAAGGC GGGAAACGAC AATCTGATCC AAGCTCAAGC TAAGCTCACG TGACGGAATT AAGCTTCGAC TTGCCTTCCG CACAATACAT CATTTCTTCT TAGCTTTTTT TCTTCTTCTT CGTTCATACA GTTTTTTTTT GTTTATCAGC TTACATTTTC TTGAACCGTA GCTTTCGTTT TCTTCTTTTT AACTTTCCAT TCGGAGTTTT TGTATCTTGT TTCATAGTTT GTCCCAGGAT TAGAATGATT AGGCATCGAA CCTTCAAGAA TTTGATTGAA TAAAACATCT TCATTCTTAA GATATGAAGA TAATCTTCAA AAGGCCCCTG GGAATCTGAA AGAAGAGAAG CAGGCCCATT TATATGGGAA AGAACAATAG TATTTCTTAT ATAGGCCCAT TTAAGTTGAA AACAATCTTC AAAAGTCCCA CATCGCTTAG ATAAGAAAAC GAAGCTGAGT TTATATACAG CTAGAGTCGA AGTAGTGATT GGGAGACCAA CCCAGTGGAC ATAAGCCTGT TCGGTTCGTA AGCTGTAATG CAAGTAGCGT ATGCGCTCAC GCAACTGGTC CAGAACCTTG ACCGAACGCA GCGGTGGTAA CGGCGCAGTG GCGGTTTTCA TGGCTTGTTA TGACTGTTTT TTTGGGGTAC AGTCTATGCC TCGGGCATCC AAGCAGCAAG CGCGTTACGC CGTGGGTCGA TGTTTGATGT TATGGAGCAG CAACGATGTT ACGCAGCAGG GCAGTCGCCC TAAAACAAAG TTAAACATCA TGGGGGAAGC GGTGATCGCC GAAGTATCGA CTCAACTATC AGAGGTAGTT GGCGTCATCG AGCGCCATCT CGAACCGACG TTGCTGGCCG TACATTTGTA CGGCTCCGCA GTGGATGGCG GCCTGAAGCC ACACAGTGAT ATTGATTTGC TGGTTACGGT GACCGTAAGG CTTGATGAAA CAACGCGGCG AGCTTTGATC AACGACCTTT TGGAAACTTC GGCTTCCCCT GGAGAGAGCG AGATTCTCCG CGCTGTAGAA GTCACCATTG TTGTGCACGA CGACATCATT CCGTGGCGTT ATCCAGCTAA GCGCGAACTG CAATTTGGAG AATGGCAGCG CAATGACATT CTTGCAGGTA TCTTCGAGCC AGCCACGATC GACATTGATC TGGCTATCTT GCTGACAAAA GCAAGAGAAC ATAGCGTTGC CTTGGTAGGT CCAGCGGCGG AGGAACTCTT TGATCCGGTT CCTGAACAGG ATCTATTTGA GGCGCTAAAT GAAACCTTAA CGCTATGGAA CTCGCCGCCC GACTGGGCTG GCGATGAGCG AAATGTAGTG CTTACGTTGT CCCGCATTTG GTACAGCGCA GTAACCGGCA AAATCGCGCC GAAGGATGTC GCTGCCGACT GGGCAATGGA GCGCCTGCCG GCCCAGTATC AGCCCGTCAT ACTTGAAGCT AGACAGGCTT ATCTTGGACA AGAAGAAGAT CGCTTGGCCT CGCGCGCAGA TCAGTTGGAA GAATTTGTCC ACTACGTGAA AGGCGAGATC ACCAAGGTAG TCGGCAAATA ATGTCTAGCT AGAAATTCGT TCAAGCCGAC GCCGCTTCGC GGCGCGGCTT AACTCAAGCG TTAGATGCAC TAAGCACATA ATTGCTCACA GCCAAACTAT CAGGTCAAGT CTGCTTTTAT TATTTTTAAG CGTGCATAAT AAGCCGGTCT CGGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG TCCGTTATCA ACTTGAAAAA GTGGCACCGA GTCGGTGCTT TTTTTTGCAA AATTTTCCAG ATCGATTTCT TCTTCCTCTG TTCTTCGGCG TTCAATTTCT GGGGTTTTCT CTTCGTTTTC TGTAACTGAA ACCTAAAATT TGACCTAAAA AAAATCTCAA ATAATATGAT TCAGTGGTTT TGTACTTTTC AGTTAGTTGA GTTTTGCAGT TCCGATGAGA TAAACCAATA AGCTTGCATG CCTGCAGGTC AACATGGTGG AGCACGACAC ACTTGTCTAC TCCAAAAATA TCAAAGATAC AGTCTCAGAA GACCAAAGGG CAATTGAGAC TTTTCAACAA AGGGTAATAT CCGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTTATTGTG AAGATAGTGG AAAAGGAAGG TGGCTCCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCC ATCGTTGAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGATAAC ATGGTGGAGC ACGACACACT TGTCTACTCC AAAAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCAA TTGAGACTTT TCAACAAAGG GTAATATCCG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT TATTGTGAAG ATAGTGGAAA AGGAAGGTGG CTCCTACAAA TGCCATCATT GCGATAAAGG AAAGGCCATC GTTGAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCCTTC CTCTATATAA GGAAGTTCAT TTCATTTGGA GAGGACCTCG ACCTCAACAC AACATATACA AAACAAACGA ATCTCAAGCA ATCAAGCATT CTACTTCTAT TGCAGCAATT TAAATCATTT CTTTTAAAGC AAAAGCAATT TTCTGAAAAT TTTCACCATT TACGAACGAT ACTCGAGGGG GATCCCCAAT ACTTGTATGG CCGCGGCCGC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTGC GATTACAAGG ACCACGACGG GGATTACAAG GACCACGACA TTGATTACAA GGATGATGAT GACAAGATGG CTCCGAAGAA GAAGAGGAAG GTTGGCATCC ACGGGGTGCC AGCTGCTGAC AAGAAGTACT CGATCGGCCT CGATATTGGG ACTAACTCTG TTGGCTGGGC CGTGATCACC GACGAGTACA AGGTGCCCTC AAAGAAGTTC AAGGTCCTGG GCAACACCGA TCGGCATTCC ATCAAGAAGA ATCTCATTGG CGCTCTCCTG TTCGACAGCG GCGAGACGGC TGAGGCTACG CGGCTCAAGC GCACCGCCCG CAGGCGGTAC ACGCGCAGGA AGAATCGCAT CTGCTACCTG CAGGAGATTT TCTCCAACGA GATGGCGAAG GTTGACGATT CTTTCTTCCA CAGGCTGGAG GAGTCATTCC TCGTGGAGGA GGATAAGAAG CACGAGCGGC ATCCAATCTT CGGCAACATT GTCGACGAGG TTGCCTACCA CGAGAAGTAC CCTACGATCT ACCATCTGCG GAAGAAGCTC GTGGACTCCA CAGATAAGGC GGACCTCCGC CTGATCTACC TCGCTCTGGC CCACATGATT AAGTTCAGGG GCCATTTCCT GATCGAGGGG GATCTCAACC CGGACAATAG CGATGTTGAC AAGCTGTTCA TCCAGCTCGT GCAGACGTAC AACCAGCTCT TCGAGGAGAA CCCCATTAAT GCGTCAGGCG TCGACGCGAA GGCTATCCTG TCCGCTAGGC TCTCGAAGTC TCGGCGCCTC GAGAACCTGA TCGCCCAGCT GCCGGGCGAG AAGAAGAACG GCCTGTTCGG GAATCTCATT GCGCTCAGCC TGGGGCTCAC GCCCAACTTC AAGTCGAATT TCGATCTCGC TGAGGACGCC AAGCTGCAGC TCTCCAAGGA CACATACGAC GATGACCTGG ATAACCTCCT GGCCCAGATC GGCGATCAGT ACGCGGACCT GTTCCTCGCT GCCAAGAATC TGTCGGACGC CATCCTCCTG TCTGATATTC TCAGGGTGAA CACCGAGATT ACGAAGGCTC CGCTCTCAGC CTCCATGATC AAGCGCTACG ACGAGCACCA TCAGGATCTG ACCCTCCTGA AGGCGCTGGT CAGGCAGCAG CTCCCCGAGA AGTACAAGGA GATCTTCTTC GATCAGTCGA AGAACGGCTA CGCTGGGTAC ATTGACGGCG GGGCCTCTCA GGAGGAGTTC TACAAGTTCA TCAAGCCGAT TCTGGAGAAG ATGGACGGCA CGGAGGAGCT GCTGGTGAAG CTCAATCGCG AGGACCTCCT GAGGAAGCAG CGGACATTCG ATAACGGCAG CATCCCACAC CAGATTCATC TCGGGGAGCT GCACGCTATC CTGAGGAGGC AGGAGGACTT CTACCCTTTC CTCAAGGATA ACCGCGAGAA GATCGAGAAG ATTCTGACTT TCAGGATCCC GTACTACGTC GGCCCACTCG CTAGGGGCAA CTCCCGCTTC GCTTGGATGA CCCGCAAGTC AGAGGAGACG ATCACGCCGT GGAACTTCGA GGAGGTGGTC GACAAGGGCG CTAGCGCTCA GTCGTTCATC GAGAGGATGA CGAATTTCGA CAAGAACCTG CCAAATGAGA AGGTGCTCCC TAAGCACTCG CTCCTGTACG AGTACTTCAC AGTCTACAAC GAGCTGACTA AGGTGAAGTA TGTGACCGAG GGCATGAGGA AGCCGGCTTT CCTGTCTGGG GAGCAGAAGA AGGCCATCGT GGACCTCCTG TTCAAGACCA ACCGGAAGGT CACGGTTAAG CAGCTCAAGG AGGACTACTT CAAGAAGATT GAGTGCTTCG ATTCGGTCGA GATCTCTGGC GTTGAGGACC GCTTCAACGC CTCCCTGGGG ACCTACCACG ATCTCCTGAA GATCATTAAG GATAAGGACT TCCTGGACAA CGAGGAGAAT GAGGATATCC TCGAGGACAT TGTGCTGACA CTCACTCTGT TCGAGGACCG GGAGATGATC GAGGAGCGCC TGAAGACTTA CGCCCATCTC TTCGATGACA AGGTCATGAA GCAGCTCAAG AGGAGGAGGT ACACCGGCTG GGGGAGGCTG AGCAGGAAGC TCATCAACGG CATTCGGGAC AAGCAGTCCG GGAAGACGAT CCTCGACTTC CTGAAGAGCG ATGGCTTCGC GAACCGCAAT TTCATGCAGC TGATTCACGA TGACAGCCTC ACATTCAAGG AGGATATCCA GAAGGCTCAG GTGAGCGGCC AGGGGGACTC GCTGCACGAG CATATCGCGA ACCTCGCTGG CTCGCCAGCT ATCAAGAAGG GGATTCTGCA GACCGTGAAG GTTGTGGACG AGCTGGTGAA GGTCATGGGC AGGCACAAGC CTGAGAACAT CGTCATTGAG ATGGCCCGGG AGAATCAGAC CACGCAGAAG GGCCAGAAGA ACTCACGCGA GAGGATGAAG AGGATCGAGG AGGGCATTAA GGAGCTGGGG TCCCAGATCC TCAAGGAGCA CCCGGTGGAG AACACGCAGC TGCAGAATGA GAAGCTCTAC CTGTACTACC TCCAGAATGG CCGCGATATG TATGTGGACC AGGAGCTGGA TATTAACAGG CTCAGCGATT ACGACGTCGA TCATATCGTT CCACAGTCAT TCCTGAAGGA TGACTCCATT GACAACAAGG TCCTCACCAG GTCGGACAAG AACCGGGGCA AGTCTGATAA TGTTCCTTCA GAGGAGGTCG TTAAGAAGAT GAAGAACTAC TGGCGCCAGC TCCTGAATGC CAAGCTGATC ACGCAGCGGA AGTTCGATAA CCTCACAAAG GCTGAGAGGG GCGGGCTCTC TGAGCTGGAC AAGGCGGGCT TCATCAAGAG GCAGCTGGTC GAGACACGGC AGATCACTAA GCACGTTGCG CAGATTCTCG ACTCACGGAT GAACACTAAG TACGATGAGA ATGACAAGCT GATCCGCGAG GTGAAGGTCA TCACCCTGAA GTCAAAGCTC GTCTCCGACT TCAGGAAGGA TTTCCAGTTC TACAAGGTTC GGGAGATCAA CAATTACCAC CATGCCCATG ACGCGTACCT GAACGCGGTG GTCGGCACAG CTCTGATCAA GAAGTACCCA AAGCTCGAGA GCGAGTTCGT GTACGGGGAC TACAAGGTTT ACGATGTGAG GAAGATGATC GCCAAGTCGG AGCAGGAGAT TGGCAAGGCT ACCGCCAAGT ACTTCTTCTA CTCTAACATT ATGAATTTCT TCAAGACAGA GATCACTCTG GCCAATGGCG AGATCCGGAA GCGCCCCCTC ATCGAGACGA ACGGCGAGAC GGGGGAGATC GTGTGGGACA AGGGCAGGGA TTTCGCGACC GTCAGGAAGG TTCTCTCCAT GCCACAAGTG AATATCGTCA AGAAGACAGA GGTCCAGACT GGCGGGTTCT CTAAGGAGTC AATTCTGCCT AAGCGGAACA GCGACAAGCT CATCGCCCGC AAGAAGGACT GGGATCCGAA GAAGTACGGC GGGTTCGACA GCCCCACTGT GGCCTACTCG GTCCTGGTTG TGGCGAAGGT TGAGAAGGGC AAGTCCAAGA AGCTCAAGAG CGTGAAGGAG CTGCTGGGGA TCACGATTAT GGAGCGCTCC AGCTTCGAGA AGAACCCGAT CGATTTCCTG GAGGCGAAGG GCTACAAGGA GGTGAAGAAG GACCTGATCA TTAAGCTCCC CAAGTACTCA CTCTTCGAGC TGGAGAACGG CAGGAAGCGG ATGCTGGCTT CCGCTGGCGA GCTGCAGAAG GGGAACGAGC TGGCTCTGCC GTCCAAGTAT GTGAACTTCC TCTACCTGGC CTCCCACTAC GAGAAGCTCA AGGGCAGCCC CGAGGACAAC GAGCAGAAGC AGCTGTTCGT CGAGCAGCAC AAGCATTACC TCGACGAGAT CATTGAGCAG ATTTCCGAGT TCTCCAAGCG CGTGATCCTG GCCGACGCGA ATCTGGATAA GGTCCTCTCC GCGTACAACA AGCACCGCGA CAAGCCAATC AGGGAGCAGG CTGAGAATAT CATTCATCTC TTCACCCTGA CGAACCTCGG CGCCCCTGCT GCTTTCAAGT ACTTCGACAC AACTATCGAT CGCAAGAGGT ACACAAGCAC TAAGGAGGTC CTGGACGCGA CCCTCATCCA CCAGTCGATT ACCGGCCTCT ACGAGACGCG CATCGACCTG TCTCAGCTCG GGGGCGACAA GCGGCCAGCG GCGACGAAGA AGGCGGGGCA GGCGAAGAAG AAGAAGTGAG CTCAGGCCTC CATACAAGTA TTGGGGATCC ACTAGCGAAT TTCCCCGATC GTTCAAACAT TTGGCAATAA AGTTTCTTAA GATTGAATCC TGTTGCCGGT CTTGCGATGA TTATCATATA ATTTCTGTTG AATTACGTTA AGCATGTAAT AATTAACATG TAATGCATGA CGTTATTTAT GAGATGGGTT TTTATGATTA GAGTCCCGCA ATTATACATT TAATACGCGA TAGAAAACAA AATATAGCGC GCAAACTAGG ATAAATTATC GCGCGCGGTG TCATCTATGT TACTAGATCG GAATTCGAGG CGGTTTGCGT ATTGGCTAGA GCAGCTTGCC AACATGGTGG AGCACGACAC TCTCGTCTAC TCCAAGAATA TCAAAGATAC AGTCTCAGAA GACCAAAGGG CTATTGAGAC TTTTCAACAA AGGGTAATAT CGGGAAACCT CCTCGGATTC CATTGCCCAG CTATCTGTCA CTTCATCAAA AGGACAGTAG AAAAGGAAGG TGGCACCTAC AAATGCCATC ATTGCGATAA AGGAAAGGCT ATCGTTCAAG ATGCCTCTGC CGACAGTGGT CCCAAAGATG GACCCCCACC CACGAGGAGC ATCGTGGAAA AAGAAGACGT TCCAACCACG TCTTCAAAGC AAGTGGATTG ATGTGATAAC ATGGTGGAGC ACGACACTCT CGTCTACTCC AAGAATATCA AAGATACAGT CTCAGAAGAC CAAAGGGCTA TTGAGACTTT TCAACAAAGG GTAATATCGG GAAACCTCCT CGGATTCCAT TGCCCAGCTA TCTGTCACTT CATCAAAAGG ACAGTAGAAA AGGAAGGTGG CACCTACAAA TGCCATCATT GCGATAAAGG AAAGGCTATC GTTCAAGATG CCTCTGCCGA CAGTGGTCCC AAAGATGGAC CCCCACCCAC GAGGAGCATC GTGGAAAAAG AAGACGTTCC AACCACGTCT TCAAAGCAAG TGGATTGATG TGATATCTCC ACTGACGTAA GGGATGACGC ACAATCCCAC TATCCTTCGC AAGACCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGGACACGCT GAAATCACCA GTCTCTCTCT ACAAATCTAT CTCTCTCGAG CTTTCGCAGA TCCCGGGGGG CAATGAGATA TGAAAAAGCC TGAACTCACC GCGACGTCTG TCGAGAAGTT TCTGATCGAA AAGTTCGACA GCGTCTCCGA CCTGATGCAG CTCTCGGAGG GCGAAGAATC TCGTGCTTTC AGCTTCGATG TAGGAGGGCG TGGATATGTC CTGCGGGTAA ATAGCTGCGC CGATGGTTTC TACAAAGATC GTTATGTTTA TCGGCACTTT GCATCGGCCG CGCTCCCGAT TCCGGAAGTG CTTGACATTG GGGAGTTTAG CGAGAGCCTG ACCTATTGCA TCTCCCGCCG TGCACAGGGT GTCACGTTGC AAGACCTGCC TGAAACCGAA CTGCCCGCTG TTCTACAACC GGTCGCGGAG GCTATGGATG CGATCGCTGC GGCCGATCTT AGCCAGACGA GCGGGTTCGG CCCATTCGGA CCGCAAGGAA TCGGTCAATA CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCAC GACTCCGGGC GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGA AATAGAGTAG ATGCCGACCG GATCTGTCGA TCGACAAGCT CGAGTTTCTC CATAATAATG TGTGAGTAGT TCCCAGATAA GGGAATTAGG GTTCCTATAG GGTTTCGCTC ATGTGTTGAG CATATAAGAA ACCCTTAGTA TGTATTTGTA TTTGTAAAAT ACTTCTATCA ATAAAATTTC TAATTCCTAA AACCAAAATC CAGTACTAAA ATCCAGATCC CCCGAATTAA TTCGGCGTTA ATTCAGTACA TTAAAAACGT CCGCAATGTG TTATTAAGTT GTCACTAGTC AGGTTAACTC AATTCGGCGT TAATTCAGTA CATTAAAAAC GTCCGCAATG TGTTATTAAG TTGTCTAAGC GTCAATTTGT TTACACCACA ATATATCCTG CCACCAGCCA GCCAACAGCT CCCCGACCGG CAGCTCGGCA CAAAATCACC ACTCGATACA GGCAGCCCAT CAGTCCGGGA CGGCGTCAGC GGGAGAGCCG TTGTAAGGCG GCAGACTTTG CTCATGTTAC CGATGCTATT CGGAAGAACG GCAACTAAGC TGCCGGGTTT GAAACACGGA TGATCTCGCG GAGGGTAGCA TGTTGATTGT AACGATGACA GAGCGTTGCT GCCTGTGATC ACTTAAGTAA CTAACTAACA GGAAGAGTTT GTAGAAACGC AAAAAGGCCA TCCGTCAGGA TGGCCTTCTG CTTAGTTTGA TGCCTGGCAG TTTATGGCGG GCGTCCTGCC CGCCACCCTC CGGGCCGTTG CTTCACAACG TTCAAATCCG CTCCCGGCGG ATTTGTCCTA CTCAGGAGAG CGTTCACCGA CAAACAACAG ATAAAACGAA AGGCCCAGTC TTCCGACTGA GCCTTTCGTT TTATTTGATG CCTGGCAGTT CCCTACTCTC GCTTAGTAGT TAGACGTCCC CGAGATCCAT GCTAGACCAT GAATCCAGAA GCCCGAGAGG TTGCCGCCTT TCGGGCTTTT TCTTTTTCAA AAAAAAAAAT TTATAAAACG ATCTGTTGCG GCCGGCCGCC GGGTTGTGGG CAAAGATGAA GGTTATCGCT GTACTTAACC AGAAAGGCGG GTCAGGCAAG ACGACCATCG CAACCCATCT AGCCCGCGCC CTGCAACTCG CCGGGGCCGA TGTTCTGTTA GTCGATTCCG ATCCCCAGGG CAGTGCCCGC GATTGGGCGG CCGTGCGGGA AGATCAACCG CTAACCGTTG TCGGCATCGA CCGCCCGACG ATTGACCGCG ACGTGAAGGC CATCGGCCGG CGCGACTTCG TAGTGATCGA CGGAGCGCCC CAGGCGGCGG ACTTGGCTGT GTCCGCGATC AAGGCAGCCG ACTTCGTGCT GATTCCGGTG CAGCCAAGCC CTTACGACAT ATGGGCCACC GCCGACCTGG TGGAGCTGGT TAAGCAGCGC ATTGAGGTCA CGGATGGAAG GCTACAAGCG GCCTTTGTCG TGTCGCGGGC GATCAAAGGC ACGCGCATCG GCGGTGAGGT TGCCGAGGCG CTGGCCGGGT ACGAGCTGCC CATTCTTGAG TCCCGTATCA CGCAGCGCGT GAGCTACCCA GGCACTGCCG CCGCCGGCAC AACCGTTCTT GAATCAGAAC CCGAGGGCGA CGCTGCCCGC GAGGTCCAGG CGCTGGCCGC TGAAATTAAA TCAAAACTCA TTTGAGTTAA TGAGGTAAAG AGAAAATGAG CAAAAGCACA AACACGCTAA GTGCCGGCCG TCCGAGCGCA CGCAGCAGCA AGGCTGCAAC GTTGGCCAGC CTGGCAGACA CGCCAGCCAT GAAGCGGGTC AACTTTCAGT TGCCGGCGGA GGATCACACC AAGCTGAAGA TGTACGCGGT ACGCCAAGGC AAGACCATTA CCGAGCTGCT ATCTGAATAC ATCGCGCAGC TACCAGAGTA AATGAGCAAA TGAATAAATG AGTAGATGAA TTTTAGCGGC TAAAGGAGGC GGCATGGAAA ATCAAGAACA ACCAGGCACC GACGCCGTGG AATGCCCCAT GTGTGGAGGA ACGGGCGGTT GGCCAGGCGT AAGCGGCTGG GTTGTCTGCC GGCCCTGCAA TGGCACTGGA ACCCCCAAGC CCGAGGAATC GGCGTGACGG TCGCAAACCA TCCGGCCCGG TACAAATCGG CGCGGCGCTG GGTGATGACC TGGTGGAGAA GTTGAAGGCC GCGCAGGCCG CCCAGCGGCA ACGCATCGAG GCAGAAGCAC GCCCCGGTGA ATCGTGGCAA GCGGCCGCTG ATCGAATCCG CAAAGAATCC CGGCAACCGC CGGCAGCCGG TGCGCCGTCG ATTAGGAAGC CGCCCAAGGG CGACGAGCAA CCAGATTTTT TCGTTCCGAT GCTCTATGAC GTGGGCACCC GCGATAGTCG CAGCATCATG GACGTGGCCG TTTTCCGTCT GTCGAAGCGT GACCGACGAG CTGGCGAGGT GATCCGCTAC GAGCTTCCAG ACGGGCACGT AGAGGTTTCC GCAGGGCCGG CCGGCATGGC CAGTGTGTGG GATTACGACC TGGTACTGAT GGCGGTTTCC CATCTAACCG AATCCATGAA CCGATACCGG GAAGGGAAGG GAGACAAGCC CGGCCGCGTG TTCCGTCCAC ACGTTGCGGA CGTACTCAAG TTCTGCCGGC GAGCCGATGG CGGAAAGCAG AAAGACGACC TGGTAGAAAC CTGCATTCGG TTAAACACCA CGCACGTTGC CATGCAGCGT ACGAAGAAGG CCAAGAACGG CCGCCTGGTG ACGGTATCCG AGGGTGAAGC CTTGATTAGC CGCTACAAGA TCGTAAAGAG CGAAACCGGG CGGCCGGAGT ACATCGAGAT CGAGCTAGCT GATTGGATGT ACCGCGAGAT CACAGAAGGC AAGAACCCGG ACGTGCTGAC GGTTCACCCC GATTACTTTT TGATCGATCC CGGCATCGGC CGTTTTCTCT ACCGCCTGGC ACGCCGCGCC GCAGGCAAGG CAGAAGCCAG ATGGTTGTTC AAGACGATCT ACGAACGCAG TGGCAGCGCC GGAGAGTTCA AGAAGTTCTG TTTCACCGTG CGCAAGCTGA TCGGGTCAAA TGACCTGCCG GAGTACGATT TGAAGGAGGA GGCGGGGCAG GCTGGCCCGA TCCTAGTCAT GCGCTACCGC AACCTGATCG AGGGCGAAGC ATCCGCCGGT TCCTAATGTA CGGAGCAGAT GCTAGGGCAA ATTGCCCTAG CAGGGGAAAA AGGTCGAAAA TTTCCTGTGG ATAGCACGTA CATTGGGAAC CCAAAGCCGT ACATTGGGAA CCGGAACCCG TACATTGGGA ACCCAAAGCC GTACATTGGG AACCGGTCAC ACATGTAAGT GACTGATATA AAAGAGAAAA AAGGCGATTT TTCCGCCTAA AACTCTTTAA AACTTATTAA AACTCTTAAA ACCCGCCTGG CCTGTGCATA ACTGTCTGGC CAGCGCACAG CCGAAGAGCT GCAAAAAGCG CCTACCCTTC GGTCGCTGCG CTCCCTACGC CCCGCCGCTT CGCGTCGGCC TATCGCGGCC GCTGGCCGCT CAAAAATGGC TGGCCTACGG CCAGGCAATC TACCAGGGCG CGGACAAGCC GCGCCGTCGC CACTCGACCG CCGGCGCCCA CATCAAGGCA CCCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGG GCCCAATCTG AATAATGTTA CAACCAATTA ACCAATTCTG ATTAGAAAAA CTCATCGAGC ATCAAATGAA ACTGCAATTT ATTCATATCA GGATTATCAA TACCATATTT TTGAAAAAGC CGTTTCTGTA ATGAAGGAGA AAACTCACCG AGGCAGTTCC ATAGGATGGC AAGATCCTGG TATCGGTCTG CGATTCCGAC TCGTCCAACA TCAATACAAC CTATTAATTT CCCCTCGTCA AAAATAAGGT TATCAAGTGA GAAATCACCA TGAGTGACGA CTGAATCCGG TGAGAATGGC AAAAGTTTAT GCATTTCTTT CCAGACTTGT TCAACAGGCC AGCCATTACG CTCGTCATCA AAATCACTCG CATCAACCAA ACCGTTATTC ATTCGTGATT GCGCCTGAGC GAGACGAAAT ACGCGATCGC TGTTAAAAGG ACAATTACAA ACAGGAATCG AATGCAACCG GCGCAGGGAC ACTGCCAGCG CATCAACAAT ATTTTCACCT GAATCAGGAT ATTCTTCTAA TACCTGGAAT GCTGTTTTTC CGGGGATCGC AGTGGTGAGT AACCATGCAT CATCAGGAGT ACGGATAAAA TGCTTGATGG TCGGAAGAGG CATAAATTCC GTCAGCCAGT TTAGTCTGAC CATCTCATCT GTAACATCAT TGGCAACGCT ACCTTTGCCA TGTTTCAGAA ACAACTCTGG CGCATCGGGC TTCCCATACA AGCGATAGAT TGTCGCACCT GATTGCCCGA CATTATCGCG AGCCCATTTA TACCCATATA AATCAGCATC CATGTTGGAA TTTAATCGCG GCCTCGACGT TTCCCGTTGA ATATGGCTCA TAACACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT TATTGTTCAT GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC GGGCCAGAGC TGCAGTTTGA TCCCGAGGGG AACCCTGTGG TTGACATGCA CATACAAATG GACGAACGGA TAAACCTTTT CACGCCCTTT TAAATATCCG TTATTCTAAT AAACGCTCTT TTCTCTTAG
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