p21-promoter(human)-EGFP
$99.00
In stock
SKU
PL72731
WAF1 promoter-EGFP
100μg liquid plasmid(Instantly):$120
| name | p21-promoter(human)-EGFP | another name | ap21-promoter(human)-EGFP |
| Insert Size (bp) | 2336bp | Plasmid host | Mammary cell |
| Plasmid uses | Promoter detection | Fragment type | Promoter |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | Green |
| Promoter | p21 | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA TGGAGTTCCG CTCGAGCTCA AGCTTCGAAT TCGATATCAA GCTTGGGCAG CAGGCTGTGG CTCTGATTGG CTTTCTGGCC ATTAGGAACA TGTCCCAACA TGTTGAGCTC TGGCATAGAA GAGGCTGGTG GCTATTTTGT CCTTGGGCTG CCTGTTATCA GGTGAGGAAG GGGATGGTAG GAGACAGAAG ACCTCTAAAG GCCCCAGGTA AGCCTTAGCC TGTTACTCTG AACAGGGTAT GTGATCTGCC AGCAGATCCT TGCGACAGGG CTGGGATCTG ATGCATGTGT GCTTGTGTGA GTGTGTGCTG GGAGTCAGAT TCTGTGTGTG ACTTTTAACA GCCTGCTCCC TTGCCTTCCT CAGGGCAGAA GTCCTCCCTT AGAGTGTGTC TGGGTACACA TTCAAGTGCA TGGTTGCAAA CTTTTTTTTT TAAAGCACTG AATAGTACTA GACACTTAGT AGGTACTTAA GAAATATTGA ATGTCGTGGT GGTGGTGAGC TAGAAGTTAT AAAAAAAATT CTTTCCCAAA AACAACAACA AAAAGAATTA TTTCATTGTG AAGCTCAGTA CCACAAAAAT TTAAATAATT CATTACAAGC CTTCATTAAA AAAAAATTTC TCCCCGAAGT AAACAGACAG ACAATGTCTA GTCTATTTGA AATGCCTGAA AGCAGAGGGG CTTCAAGGCA GTGGGAGAAG GTGCCTGTCC TCTGCTGGAC ATTTGACAAC CAGCCCTTTG GATGGCTTGT ATGTATAGGA GCGAAGGTGC AGACAGCAGT GGGGCTTAGA GTGGGGTCCT GAGGCTGTGC TGTGGCCCTT CTGGGGTTTA GCCACAATCC TGGCCTGACT CCAGGGCGAG GCAGGCCAAG GGGGCCTGCT GCTGTGTCCT CCCACCCCTA CCTGGGCTCC CATCCCCACA GCAGAGGAGA AAGAAGCCTG TCCTCCCCGA GGTCAGCTGC GTTAGAGGAA GAAGACTGGG CATGTCTGGG CAGAGATTTC CAGACTCTGA GCAGCCTGAG ATGTCAGTAA TTGTAGCTGC TCCAAGCCTG GGTTCTGTTT TTCAGTGGGA TTTCTGTTCA GATGAACAAT CCATCCTCTG CAATTTTTTA AAAGCAAAAC TGCAAATGTT TCAGGCACAG AAAGGAGGCA AAGGTGAAGT CCAGGGGAGG TCAGGGGTGT GAGGTAGATG GGAGCGGATA GGCACATCAC TCATTTCTGT GTCTGTCAGA AGAACCAGTA GACACTTCCA GAATTGTCCT TTATTTACGT CATCTCCATA AACCATCTGC AAATGAGGGT TATTTGGCAT TTTTGTCATT TTGGAACCAC AGAAATAAAG GATGACAAGC AGAGAGCCCC GGGCAGGAGG CAAAAGTCCT GTGTTCCAAC TATAGTCATT TCTTTGCTGC ATGATCTGAG TTAGGTCACC AGACTTCTCT GAGCCCCAGT TTCCCCAGCA GTGTATACGG GCTATGTGGG GAGTATTCAG GAGACAGACA ACTCACTCGT CAAATCCTCC CCTTCCTGGC CAACAAAGCT GCTGCAACCA CAGGGGTTTC TTCTGTTCAG GTGAGTGTAG GGTGTAGGGA GATTGGTTCA ATGTCCAATT CTTCTGTTTC CCTGGAGATC AGGTTGCCCT TTTTTGGTAG TCTCTCCAAT TCCCTCCTTC CCGGAAGCAT GTGACAATCA ACAACTTTGT ATACTTAAGT TCAGTGGACC TCAATTTCCT CATCTGTGAA ATAAACGGGA CTGAAAAATC ATTCCGGCCT CAAGATGCTT TGTTGGGGTG TCTAGGTGCT CCAGGTGCTT CTGGGAGAGG TGACCTAGTG AGGGATCAGT GGGAATAGAG GTGATATTGT GGGGCTTTTC TGGAAATTGC AGAGAGGTGC ATCGTTTTTA TAATTTATGA ATTTTTATGT ATTAATGTCA TCCTCCTGAT CTTTTCAGCT GCATTGGGTA AATCCTTGCC TGCCAGAGTG GGTCAGCGGT GAGCCAGAAA GGGGGCTCAT TCTAACAGTG CTGTGTCCTC CTGGAGAGTG CCAACTCATT CTCCAAGTAA AAAAAGCCAG ATTTGTGGCT CACTTCGTGG GGAAATGTGT CCAGCGCACC AACGCAGGCG AGGGACTGGG GGAGGAGGGA AGTGCCCCTC TGCAGCACGC GAGGTTCCGG GACCGGCTGG CCTGCTGGAA CTCGGCCAGG CTCAGCTGGC TCCGCGCTGG GCAGCCAGGA GCCTGGGCCC CGGGGAGGGC GGTCCCGGGC GGCGCGGTGG GCCGAGCGCG GGTCCCGCCT CCTTGAGGCG GGCCCGGGCG GGGCGGTTGT ATATCAGGGC CGCGCAGAGC TGCGCCAGCT GAGGTGTGAG CAGCTGCCGA AGTCAGAAGC TTATCGATAC CGTCGACGGT ACCGCGGGCC CGGGATCCAC CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AAGCGGCCGC GACTCTAGAT CATAATCAGC CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAAGGCG TAAATTGTAA GCGTTAATAT TTTGTTAAAA TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC ATGCAT
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