pLV3-CMV-KIF2C(human)-3×HA-CopGFP-Puro
$84.00
In stock
SKU
PL71631
NM_006845.4;CT139; KNSL6; MCAK
100μg liquid plasmid(Instantly):$105
| name | pLV3-CMV-KIF2C(human)-3×HA-CopGFP-Puro | another name | apLV3-CMV-KIF2C(human)-3×HA-CopGFP-Puro |
| Insert Size (bp) | 2178bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Green |
| Promoter | CMV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F： | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGGTGGCGA TTCGGCACGA GGGCGAATTG AGGTTTCTTG GTATTGCGCG TTTCTCTTCC TTGCTGACTC TCCGAATGGC CATGGACTCG TCGCTTCAGG CCCGCCTGTT TCCCGGTCTC GCTATCAAGA TCCAACGCAG TAATGGTTTA ATTCACAGTG CCAATGTAAG GACTGTGAAC TTGGAGAAAT CCTGTGTTTC AGTGGAATGG GCAGAAGGAG GTGCCACAAA GGGCAAAGAG ATTGATTTTG ATGATGTGGC TGCAATAAAC CCAGAACTCT TACAGCTTCT TCCCTTACAT CCGAAGGACA ATCTGCCCTT GCAGGAAAAT GTAACAATCC AGAAACAAAA ACGGAGATCC GTCAACTCCA AAATTCCTGC TCCAAAAGAA AGTCTTCGAA GCCGCTCCAC TCGCATGTCC ACTGTCTCAG AGCTTCGCAT CACGGCTCAG GAGAATGACA TGGAGGTGGA GCTGCCTGCA GCTGCAAACT CCCGCAAGCA GTTTTCAGTT CCTCCTGCCC CCACTAGGCC TTCCTGCCCT GCAGTGGCTG AAATACCATT GAGGATGGTC AGCGAGGAGA TGGAAGAGCA AGTCCATTCC ATCCGAGGCA GCTCTTCTGC AAACCCTGTG AACTCAGTTC GGAGGAAATC ATGTCTTGTG AAGGAAGTGG AAAAAATGAA GAACAAGCGA GAAGAGAAGA AGGCCCAGAA CTCTGAAATG AGAATGAAGA GAGCTCAGGA GTATGACAGT AGTTTTCCAA ACTGGGAATT TGCCCGAATG ATTAAAGAAT TTCGGGCTAC TTTGGAATGT CATCCACTTA CTATGACTGA TCCTATCGAA GAGCACAGAA TATGTGTCTG TGTTAGGAAA CGCCCACTGA ATAAGCAAGA ATTGGCCAAG AAAGAAATTG ATGTGATTTC CATTCCTAGC AAGTGTCTCC TCTTGGTACA TGAACCCAAG TTGAAAGTGG ACTTAACAAA GTATCTGGAG AACCAAGCAT TCTGCTTTGA CTTTGCATTT GATGAAACAG CTTCGAATGA AGTTGTCTAC AGGTTCACAG CAAGGCCACT GGTACAGACA ATCTTTGAAG GTGGAAAAGC AACTTGTTTT GCATATGGCC AGACAGGAAG TGGCAAGACA CATACTATGG GCGGAGACCT CTCTGGGAAA GCCCAGAATG CATCCAAAGG GATCTATGCC ATGGCCTCCC GGGACGTCTT CCTCCTGAAG AATCAACCCT GCTACCGGAA GTTGGGCCTG GAAGTCTATG TGACATTCTT CGAGATCTAC AATGGGAAGC TGTTTGACCT GCTCAACAAG AAGGCCAAGC TGCGCGTGCT GGAGGACGGC AAGCAACAGG TGCAAGTGGT GGGGCTGCAG GAGCATCTGG TTAACTCTGC TGATGATGTC ATCAAGATGA TCGACATGGG CAGCGCCTGC AGAACCTCTG GGCAGACATT TGCCAACTCC AATTCCTCCC GCTCCCACGC GTGCTTCCAA ATTATTCTTC GAGCTAAAGG GAGAATGCAT GGCAAGTTCT CTTTGGTAGA TCTGGCAGGG AATGAGCGAG GCGCGGACAC TTCCAGTGCT GACCGGCAGA CCCGCATGGA GGGCGCAGAA ATCAACAAGA GTCTCTTAGC CCTGAAGGAG TGCATCAGGG CCCTGGGACA GAACAAGGCT CACACCCCGT TCCGTGAGAG CAAGCTGACA CAGGTGCTGA GGGACTCCTT CATTGGGGAG AACTCTAGGA CTTGCATGAT TGCCACGATC TCACCAGGCA TAAGCTCCTG TGAATATACT TTAAACACCC TGAGATATGC AGACAGGGTC AAGGAGCTGA GCCCCCACAG TGGGCCCAGT GGAGAGCAGT TGATTCAAAT GGAAACAGAA GAGATGGAAG CCTGCTCTAA CGGGGCGCTG ATTCCAGGCA ATTTATCCAA GGAAGAGGAG GAACTGTCTT CCCAGATGTC CAGCTTTAAC GAAGCCATGA CTCAGATCAG GGAGCTGGAG GAGAAGGCTA TGGAAGAGCT CAAGGAGATC ATACAGCAAG GACCAGACTG GCTTGAGCTC TCTGAGATGA CCGAGCAGCC AGACTATGAC CTGGAGACCT TTGTGAACAA AGCGGAATCT GCTCTGGCCC AGCAAGCCAA GCATTTCTCA GCCCTGCCAG ATGTCATCAA GGCCTTGCGC CTGGCCATGC AGCTGGAAGA GCAGGCTAGC AGACAAATAA GCAGCAAGAA ACGGCCCCAG TGACGACTGC AAATAAAAAT CTGTTTGGTT TGACACCCAG CCTCTTCCCT GGCCCTCCCC AGAGAACTTT GGGTACCTGG TGGGTCTAGG CAGGGTCTGA GCTGGGACAG GTTCTGGTAA ATGCCAAGTA TGGGGGCATC TGGGCCCAGG GCAGCTGGGG AGGGGGTCAG AGTGACATGG GACACTCCTT TTCTGTTCCT CAGTTGTCGC CCTCACGAGA GGAAGGAGCT CTTAGTTACC CTTTTGTGTT GCCCTTCTTT CCATCAAGGG GAATGTTCTC AGCATAGAGC TTTCTCCGCA GCATCCTGCC TGCGTGGACT GGCTGCTAAT GGAGAGCTCC CTGGGGTTGT CCTGGCTCTG GGGAGAGAGA CGGAGCCTTT AGTACAGCTA TCTGCTGGCT CTAAACCTTC TACGCCTTTG GGCCGAGCAC TGAATGTCTT GTACTTTAAA AAAATGTTTC TGAGACCTCT TTCTACTTTA CTGTCTCCCT AGAGATCCTA GAGGATCCCT ACTGTTTTCT GTTTTATGTG TTTATACATT GTATGTAACA ATAAAGAGAA AAAATAAAAA AAAAAAAAAA AAAAAAAAAA AAAACTCGAG GCATCTATGT CGGCGCGAGA CACATTATAT CCAAAACCCA AC
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