pCMV-FAF1(human)-3×HA-Neo
$91.00
In stock
SKU
PL70903
100μg liquid plasmid(Instantly):$91
| name | pCMV-FAF1(human)-3×HA-Neo | another name | apCMV-FAF1(human)-3×HA-Neo |
| Insert Size (bp) | Plasmid host | ||
| Plasmid uses | Fragment type | ||
| Fragment species | Prokaryotic resistance | ||
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | ||
| Competence | Growth Temperature | ||
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | ||
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCATGGC GTCCAACATG GACCGGGAGA TGATCCTGGC GGATTTTCAG GCATGTACTG GCATTGAAAA CATTGACGAA GCTATTACAT TGCTTGAACA AAATAATTGG GACTTAGTGG CAGCTATCAA TGGTGTAATA CCACAGGAAA ATGGCATTCT ACAAAGTGAA TATGGAGGTG AGACCATACC AGGACCTGCA TTTAATCCAG CAAGTCATCC AGCTTCAGCT CCTACTTCCT CTTCTTCTTC AGCGTTTCGA CCTGTAATGC CATCCAGGCA GATTGTAGAA AGGCAACCTC GGATGCTGGA CTTCAGGGTT GAATACAGAG ACAGAAATGT TGATGTGGTA CTTGAAGACA CCTGTACTGT TGGAGAGATT AAACAGATTC TAGAAAATGA ACTTCAGATA CCTGTGTCCA AAATGCTGTT AAAAGGCTGG AAGACGGGAG ATGTGGAAGA CAGTACGGTC CTAAAATCTC TACACTTGCC AAAAAACAAC AGTCTTTATG TCCTTACACC AGATTTGCCA CCACCTTCAT CATCTAGTCA TGCTGGTGCC CTGCAGGAGT CATTAAATCA AAACTTCATG CTGATCATCA CCCACCGAGA AGTCCAGCGG GAGTACAACC TGAACTTCTC AGGAAGCAGT ACTATTCAAG AGGTAAAGAG AAATGTGTAT GACCTTACAA GTATCCCCGT TCGCCACCAA TTATGGGAGG GCTGGCCAAC TTCTGCTACA GACGACTCAA TGTGTCTTGC TGAATCAGGG CTCTCTTATC CCTGCCATCG ACTTACAGTG GGAAGAAGAT CTTCACCTGC ACAGACCCGG GAACAGTCGG AAGAACAAAT CACCGATGTT CATATGGTTA GTGATAGCGA TGGAGATGAC TTTGAAGATG CTACAGAATT TGGGGTGGAT GATGGAGAAG TATTTGGCAT GGCGTCATCT GCCTTGAGAA AATCTCCAAT GATGCCAGAA AACGCAGAAA ATGAAGGAGA TGCCTTATTA CAATTTACAG CAGAGTTTTC TTCAAGATAT GGTGATTGCC ATCCTGTATT TTTTATTGGC TCATTAGAAG CTGCTTTTCA AGAGGCCTTC TATGTGAAAG CCCGAGATAG AAAGCTTCTT GCTATCTACC TCCACCATGA TGAAAGTGTG TTAACCAACG TGTTCTGCTC ACAAATGCTT TGTGCTGAAT CCATTGTTTC TTATCTGAGT CAAAATTTTA TAACCTGGGC TTGGGATCTG ACAAAGGACT CCAACAGAGC AAGATTTCTC ACTATGTGCA ATAGACACTT TGGCAGTGTT GTGGCACAAA CCATTCGGAC TCAAAAAACG GATCAGTTTC CGCTTTTCCT GATTATTATG GGAAAGCGAT CATCTAATGA AGTGTTGAAT GTGATACAAG GGAACACAAC AGTAGATGAG TTAATGATGA GACTCATGGC TGCAATGGAG ATCTTCACAG CCCAACAACA GGAAGATATA AAGGACGAGG ATGAACGTGA AGCCAGAGAA AATGTGAAGA GAGAGCAAGA TGAGGCCTAT CGCCTTTCAC TTGAGGCTGA CAGAGCAAAG AGGGAAGCTC ACGAGAGAGA GATGGCAGAA CAGTTTCGTT TGGAGCAGAT TCGCAAAGAA CAAGAAGAGG AACGTGAGGC CATCCGGCTG TCCTTAGAGC AAGCCCTGCC TCCTGAGCCA AAGGAAGAAA ATGCTGAGCC TGTGAGCAAA CTGCGGATCC GGACCCCCAG TGGCGAGTTC TTGGAGCGGC GTTTCCTGGC CAGCAACAAG CTCCAGATTG TCTTTGATTT TGTAGCTTCC AAAGGATTTC CATGGGATGA GTACAAGTTA CTGAGCACCT TTCCTAGGAG AGACGTAACT CAACTGGACC CAAATAAATC ATTATTGGAG GTAAAGTTGT TCCCTCAAGA AACCCTTTTC CTTGAAGCAA AAGAGCTCGA GTACCCATAC GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT GATGTCCCAG ACTACGCATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC
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