pCAMBIA1300-35S-E.R.-CFP-HDEL
$113.00
In stock
SKU
PL69914
内质网定位
100μg liquid plasmid(Instantly):$149
| name | pCAMBIA1300-35S-E.R.-CFP-HDEL | another name | apCAMBIA1300-35S-E.R.-CFP-HDEL |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Subcellular localization | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Kan | |
| Selective marker | Hyg | Fluorescent labeling | cyan |
| Promoter | 35S | Replicon | pVS1 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATTCGGTCC CCAGATTAGC CTTTTCAATT TCAGAAAGAA TGCTAACCCA CAGATGGTTA GAGAGGCTTA CGCAGCAGGT CTCATCAAGA CGATCTACCC GAGCAATAAT CTCCAGGAAA TCAAATACCT TCCCAAGAAG GTTAAAGATG CAGTCAAAAG ATTCAGGACT AACTGCATCA AGAACACAGA GAAAGATATA TTTCTCAAGA TCAGAAGTAC TATTCCAGTA TGGACGATTC AAGGCTTGCT TCACAAACCA AGGCAAGTAA TAGAGATTGG AGTCTCTAAA AAGGTAGTTC CCACTGAATC AAAGGCCATG GAGTCAAAGA TTCAAATAGA GGACCTAACA GAACTCGCCG TAAAGACTGG CGAACAGTTC ATACAGAGTC TCTTACGACT CAATGACAAG AAGAAAATCT TCGTCAACAT GGTGGAGCAC GACACACTTG TCTACTCCAA AAATATCAAA GATACAGTCT CAGAAGACCA AAGGGCAATT GAGACTTTTC AACAAAGGGT AATATCCGGA AACCTCCTCG GATTCCATTG CCCAGCTATC TGTCACTTTA TTGTGAAGAT AGTGGAAAAG GAAGGTGGCT CCTACAAATG CCATCATTGC GATAAAGGAA AGGCCATCGT TGAAGATGCC TCTGCCGACA GTGGTCCCAA AGATGGACCC CCACCCACGA GGAGCATCGT GGAAAAAGAA GACGTTCCAA CCACGTCTTC AAAGCAAGTG GATTGATGTG ATATCTCCAC TGACGTAAGG GATGACGCAC AATCCCACTA TCCTTCGCAA GACCCTTCCT CTATATAAGG AAGTTCATTT CATTTGGAGA GAACACGGGG GACGAGCTCA TGAGTCTGTT CCATGTGCTA GGGTTTGGTG TCAAGATTGG GCATCTCTTC TGGATGCTAT GCTGCTGGTT TGTTTCTTGG TTCGTTGATA ATGGGATCGA GGACAAGTCT GGTCTTTTAG TTGGCTCTGT CGGTGATCTT GAGAAGACTA AGATGACTAC GTTGAAGAAG AAGAACAAGA TGTGGTTCTG GAATAAGATC TCTAGCAGCG GACTCAAGAT CCCGAGTTTC TCTTATCAGT TTCTTGGCTC TGTTAAATTC AACAAGGCGT GGTGGAGGAA GCTTGTGGTG GTTTGGGTTG TCTTCTGGGT CTTGGTCTCT ATTTGGACGT TTTGGTACTT TAGCTCGTCT AGAGGGCCCG GCGCGCCACT AGTGTCGACC AGATCTCGTA CGCGTATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAGGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTGGGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGTACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACATCAGCC ACAACGTCTA TATCACCGCC GACAAGCAGA AGAACGGCAT CAAGGCCCAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGCAACTCGA GCATGACGAG CTGTAGCTGC AGAGCTTTCG TTCGTATCAT CGGTTTCGAC AACGTTCGTC AAGTTCAATG CATCAGTTTC ATTGCGCACA CACCAGAATC CTACTGAGTT CGAGTATTAT GGCATTGGGA AACATGTTTT TCTTGTACCA TTTGTTGTGC TTGTAATTTA CTGTGTTTTT TATTCGGTTT TCGCTATCGA ACTGTGAAAT GGAAATGGAT GGAGAAGAGT TAATGAATGA TATGGTCCTT TTGTTCATTC TCAAATTAAT ATTATTTGTT TTTTCTCTTA TTTGTTGTGT GTTGAATTTG AAAATATAAG AGATATGCAA ACATTTTGTT TTGAGTAAAA ATGTGTCAAA TCGTGGCCTC TAATGACCGA AGTTAATATG AGGAGTAAAA CACTTGTAGT TGTACCATTA TGCTTATTCA CTAGGCAACA AATATATTTT CAGACCTAGA AAAGCTGCAA ATGTTACTGA ATACAAGTAT GTCCTCTTGT GTTTTAGACA TTTATGAACT TTCCTTTATG TAATTTTCCA GAATCCTTGT CAGATTCTAA TCATTGCTTT ATAATTATAG TTATACTCAT GGATTTGTAG TTGAGTATGA AAATATTTTT TAATGCATTT TATGACTTGC CAATTGATTG ACAACATGCA TCAAGCTTGG CACTGGCCGT CGTTTTACAA CGTCGTGACT GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCCT TTCGCCAGCT GGCGTAATAG CGAAGAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG GCGAATGCTA GAGCAGCTTG AGCTTGGATC AGATTGTCGT TTCCCGCCTT CAGTTTAAAC TATCAGTGTT TGACAGGATA TATTGGCGGG TAAACCTAAG AGAAAAGAGC GTTTATTAGA ATAACGGATA TTTAAAAGGG CGTGAAAAGG TTTATCCGTT CGTCCATTTG TATGTGCATG CCAACCACAG GGTTCCCCTC GGGATCAAAG TACTTTGATC CAACCCCTCC GCTGCTATAG TGCAGTCGGC TTCTGACGTT CAGTGCAGCC GTCTTCTGAA AACGACATGT CGCACAAGTC CTAAGTTACG CGACAGGCTG CCGCCCTGCC CTTTTCCTGG CGTTTTCTTG TCGCGTGTTT TAGTCGCATA AAGTAGAATA CTTGCGACTA GAACCGGAGA CATTACGCCA TGAACAAGAG CGCCGCCGCT GGCCTGCTGG GCTATGCCCG CGTCAGCACC GACGACCAGG ACTTGACCAA CCAACGGGCC GAACTGCACG CGGCCGGCTG CACCAAGCTG TTTTCCGAGA AGATCACCGG CACCAGGCGC GACCGCCCGG AGCTGGCCAG GATGCTTGAC CACCTACGCC CTGGCGACGT TGTGACAGTG ACCAGGCTAG ACCGCCTGGC CCGCAGCACC CGCGACCTAC TGGACATTGC CGAGCGCATC CAGGAGGCCG GCGCGGGCCT GCGTAGCCTG GCAGAGCCGT GGGCCGACAC CACCACGCCG GCCGGCCGCA TGGTGTTGAC CGTGTTCGCC GGCATTGCCG AGTTCGAGCG TTCCCTAATC ATCGACCGCA CCCGGAGCGG GCGCGAGGCC GCCAAGGCCC GAGGCGTGAA GTTTGGCCCC CGCCCTACCC TCACCCCGGC ACAGATCGCG CACGCCCGCG AGCTGATCGA CCAGGAAGGC CGCACCGTGA AAGAGGCGGC TGCACTGCTT GGCGTGCATC GCTCGACCCT GTACCGCGCA CTTGAGCGCA GCGAGGAAGT GACGCCCACC GAGGCCAGGC GGCGCGGTGC CTTCCGTGAG GACGCATTGA CCGAGGCCGA CGCCCTGGCG GCCGCCGAGA ATGAACGCCA AGAGGAACAA GCATGAAACC GCACCAGGAC GGCCAGGACG AACCGTTTTT CATTACCGAA GAGATCGAGG CGGAGATGAT CGCGGCCGGG TACGTGTTCG AGCCGCCCGC GCACGTCTCA ACCGTGCGGC TGCATGAAAT CCTGGCCGGT TTGTCTGATG CCAAGCTGGC GGCCTGGCCG GCCAGCTTGG CCGCTGAAGA AACCGAGCGC CGCCGTCTAA AAAGGTGATG TGTATTTGAG TAAAACAGCT TGCGTCATGC GGTCGCTGCG TATATGATGC GATGAGTAAA TAAACAAATA CGCAAGGGGA ACGCATGAAG GTTATCGCTG TACTTAACCA GAAAGGCGGG TCAGGCAAGA CGACCATCGC AACCCATCTA GCCCGCGCCC TGCAACTCGC CGGGGCCGAT GTTCTGTTAG TCGATTCCGA TCCCCAGGGC AGTGCCCGCG ATTGGGCGGC CGTGCGGGAA GATCAACCGC TAACCGTTGT CGGCATCGAC CGCCCGACGA TTGACCGCGA CGTGAAGGCC ATCGGCCGGC GCGACTTCGT AGTGATCGAC GGAGCGCCCC AGGCGGCGGA CTTGGCTGTG TCCGCGATCA AGGCAGCCGA CTTCGTGCTG ATTCCGGTGC AGCCAAGCCC TTACGACATA TGGGCCACCG CCGACCTGGT GGAGCTGGTT AAGCAGCGCA TTGAGGTCAC GGATGGAAGG CTACAAGCGG CCTTTGTCGT GTCGCGGGCG ATCAAAGGCA CGCGCATCGG CGGTGAGGTT GCCGAGGCGC TGGCCGGGTA CGAGCTGCCC ATTCTTGAGT CCCGTATCAC GCAGCGCGTG AGCTACCCAG GCACTGCCGC CGCCGGCACA ACCGTTCTTG AATCAGAACC CGAGGGCGAC GCTGCCCGCG AGGTCCAGGC GCTGGCCGCT GAAATTAAAT CAAAACTCAT TTGAGTTAAT GAGGTAAAGA GAAAATGAGC AAAAGCACAA ACACGCTAAG TGCCGGCCGT CCGAGCGCAC GCAGCAGCAA GGCTGCAACG TTGGCCAGCC TGGCAGACAC GCCAGCCATG AAGCGGGTCA ACTTTCAGTT GCCGGCGGAG GATCACACCA AGCTGAAGAT GTACGCGGTA CGCCAAGGCA AGACCATTAC CGAGCTGCTA TCTGAATACA TCGCGCAGCT ACCAGAGTAA ATGAGCAAAT GAATAAATGA GTAGATGAAT TTTAGCGGCT AAAGGAGGCG GCATGGAAAA TCAAGAACAA CCAGGCACCG ACGCCGTGGA ATGCCCCATG TGTGGAGGAA CGGGCGGTTG GCCAGGCGTA AGCGGCTGGG TTGTCTGCCG GCCCTGCAAT GGCACTGGAA CCCCCAAGCC CGAGGAATCG GCGTGACGGT CGCAAACCAT CCGGCCCGGT ACAAATCGGC GCGGCGCTGG GTGATGACCT GGTGGAGAAG TTGAAGGCCG CGCAGGCCGC CCAGCGGCAA CGCATCGAGG CAGAAGCACG CCCCGGTGAA TCGTGGCAAG CGGCCGCTGA TCGAATCCGC AAAGAATCCC GGCAACCGCC GGCAGCCGGT GCGCCGTCGA TTAGGAAGCC GCCCAAGGGC GACGAGCAAC CAGATTTTTT CGTTCCGATG CTCTATGACG TGGGCACCCG CGATAGTCGC AGCATCATGG ACGTGGCCGT TTTCCGTCTG TCGAAGCGTG ACCGACGAGC TGGCGAGGTG ATCCGCTACG AGCTTCCAGA CGGGCACGTA GAGGTTTCCG CAGGGCCGGC CGGCATGGCC AGTGTGTGGG ATTACGACCT GGTACTGATG GCGGTTTCCC ATCTAACCGA ATCCATGAAC CGATACCGGG AAGGGAAGGG AGACAAGCCC GGCCGCGTGT TCCGTCCACA CGTTGCGGAC GTACTCAAGT TCTGCCGGCG AGCCGATGGC GGAAAGCAGA AAGACGACCT GGTAGAAACC TGCATTCGGT TAAACACCAC GCACGTTGCC ATGCAGCGTA CGAAGAAGGC CAAGAACGGC CGCCTGGTGA CGGTATCCGA GGGTGAAGCC TTGATTAGCC GCTACAAGAT CGTAAAGAGC GAAACCGGGC GGCCGGAGTA CATCGAGATC GAGCTAGCTG ATTGGATGTA CCGCGAGATC ACAGAAGGCA AGAACCCGGA CGTGCTGACG GTTCACCCCG ATTACTTTTT GATCGATCCC GGCATCGGCC GTTTTCTCTA CCGCCTGGCA CGCCGCGCCG CAGGCAAGGC AGAAGCCAGA TGGTTGTTCA AGACGATCTA CGAACGCAGT GGCAGCGCCG GAGAGTTCAA GAAGTTCTGT TTCACCGTGC GCAAGCTGAT CGGGTCAAAT GACCTGCCGG AGTACGATTT GAAGGAGGAG GCGGGGCAGG CTGGCCCGAT CCTAGTCATG CGCTACCGCA ACCTGATCGA GGGCGAAGCA TCCGCCGGTT CCTAATGTAC GGAGCAGATG CTAGGGCAAA TTGCCCTAGC AGGGGAAAAA GGTCGAAAAG GTCTCTTTCC TGTGGATAGC ACGTACATTG GGAACCCAAA GCCGTACATT GGGAACCGGA ACCCGTACAT TGGGAACCCA AAGCCGTACA TTGGGAACCG GTCACACATG TAAGTGACTG ATATAAAAGA GAAAAAAGGC GATTTTTCCG CCTAAAACTC TTTAAAACTT ATTAAAACTC TTAAAACCCG CCTGGCCTGT GCATAACTGT CTGGCCAGCG CACAGCCGAA GAGCTGCAAA AAGCGCCTAC CCTTCGGTCG CTGCGCTCCC TACGCCCCGC CGCTTCGCGT CGGCCTATCG CGGCCGCTGG CCGCTCAAAA ATGGCTGGCC TACGGCCAGG CAATCTACCA GGGCGCGGAC AAGCCGCGCC GTCGCCACTC GACCGCCGGC GCCCACATCA AGGCACCCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT GTACTGAGAG TGCACCATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGCA TTCTAGGTAC TAAAACAATT CATCCAGTAA AATATAATAT TTTATTTTCT CCCAATCAGG CTTGATCCCC AGTAAGTCAA AAAATAGCTC GACATACTGT TCTTCCCCGA TATCCTCCCT GATCGACCGG ACGCAGAAGG CAATGTCATA CCACTTGTCC GCCCTGCCGC TTCTCCCAAG ATCAATAAAG CCACTTACTT TGCCATCTTT CACAAAGATG TTGCTGTCTC CCAGGTCGCC GTGGGAAAAG ACAAGTTCCT CTTCGGGCTT TTCCGTCTTT AAAAAATCAT ACAGCTCGCG CGGATCTTTA AATGGAGTGT CTTCTTCCCA GTTTTCGCAA TCCACATCGG CCAGATCGTT ATTCAGTAAG TAATCCAATT CGGCTAAGCG GCTGTCTAAG CTATTCGTAT AGGGACAATC CGATATGTCG ATGGAGTGAA AGAGCCTGAT GCACTCCGCA TACAGCTCGA TAATCTTTTC AGGGCTTTGT TCATCTTCAT ACTCTTCCGA GCAAAGGACG CCATCGGCCT CACTCATGAG CAGATTGCTC CAGCCATCAT GCCGTTCAAA GTGCAGGACC TTTGGAACAG GCAGCTTTCC TTCCAGCCAT AGCATCATGT CCTTTTCCCG TTCCACATCA TAGGTGGTCC CTTTATACCG GCTGTCCGTC ATTTTTAAAT ATAGGTTTTC ATTTTCTCCC ACCAGCTTAT ATACCTTAGC AGGAGACATT CCTTCCGTAT CTTTTACGCA GCGGTATTTT TCGATCAGTT TTTTCAATTC CGGTGATATT CTCATTTTAG CCATTTATTA TTTCCTTCCT CTTTTCTACA GTATTTAAAG ATACCCCAAG AAGCTAATTA TAACAAGACG AACTCCAATT CACTGTTCCT TGCATTCTAA AACCTTAAAT ACCAGAAAAC AGCTTTTTCA AAGTTGTTTT CAAAGTTGGC GTATAACATA GTATCGACGG AGCCGATTTT GAAACCGCGG TGATCACAGG CAGCAACGCT CTGTCATCGT TACAATCAAC ATGCTACCCT CCGCGAGATC ATCCGTGTTT CAAACCCGGC AGCTTAGTTG CCGTTCTTCC GAATAGCATC GGTAACATGA GCAAAGTCTG CCGCCTTACA ACGGCTCTCC CGCTGACGCC GTCCCGGACT GATGGGCTGC CTGTATCGAG TGGTGATTTT GTGCCGAGCT GCCGGTCGGG GAGCTGTTGG CTGGCTGGTG GCAGGATATA TTGTGGTGTA AACAAATTGA CGCTTAGACA ACTTAATAAC ACATTGCGGA CGTTTTTAAT GTACTGAATT AACGCCGAAT TAATTCGGGG GATCTGGATT TTAGTACTGG ATTTTGGTTT TAGGAATTAG AAATTTTATT GATAGAAGTA TTTTACAAAT ACAAATACAT ACTAAGGGTT TCTTATATGC TCAACACATG AGCGAAACCC TATAGGAACC CTAATTCCCT TATCTGGGAA CTACTCACAC ATTATTATGG AGAAACTCGA GCTTGTCGAT CGACAGATCC GGTCGGCATC TACTCTATTT CTTTGCCCTC GGACGAGTGC TGGGGCGTCG GTTTCCACTA TCGGCGAGTA CTTCTACACA GCCATCGGTC CAGACGGCCG CGCTTCTGCG GGCGATTTGT GTACGCCCGA CAGTCCCGGC TCCGGATCGG ACGATTGCGT CGCATCGACC CTGCGCCCAA GCTGCATCAT CGAAATTGCC GTCAACCAAG CTCTGATAGA GTTGGTCAAG ACCAATGCGG AGCATATACG CCCGGAGTCG TGGCGATCCT GCAAGCTCCG GATGCCTCCG CTCGAAGTAG CGCGTCTGCT GCTCCATACA AGCCAACCAC GGCCTCCAGA AGAAGATGTT GGCGACCTCG TATTGGGAAT CCCCGAACAT CGCCTCGCTC CAGTCAATGA CCGCTGTTAT GCGGCCATTG TCCGTCAGGA CATTGTTGGA GCCGAAATCC GCGTGCACGA GGTGCCGGAC TTCGGGGCAG TCCTCGGCCC AAAGCATCAG CTCATCGAGA GCCTGCGCGA CGGACGCACT GACGGTGTCG TCCATCACAG TTTGCCAGTG ATACACATGG GGATCAGCAA TCGCGCATAT GAAATCACGC CATGTAGTGT ATTGACCGAT TCCTTGCGGT CCGAATGGGC CGAACCCGCT CGTCTGGCTA AGATCGGCCG CAGCGATCGC ATCCATAGCC TCCGCGACCG GTTGTAGAAC AGCGGGCAGT TCGGTTTCAG GCAGGTCTTG CAACGTGACA CCCTGTGCAC GGCGGGAGAT GCAATAGGTC AGGCTCTCGC TAAACTCCCC AATGTCAAGC ACTTCCGGAA TCGGGAGCGC GGCCGATGCA AAGTGCCGAT AAACATAACG ATCTTTGTAG AAACCATCGG CGCAGCTATT TACCCGCAGG ACATATCCAC GCCCTCCTAC ATCGAAGCTG AAAGCACGAG ATTCTTCGCC CTCCGAGAGC TGCATCAGGT CGGAGACGCT GTCGAACTTT TCGATCAGAA ACTTCTCGAC AGACGTCGCG GTGAGTTCAG GCTTTTTCAT ATCTCATTGC CCCCCGGGAT CTGCGAAAGC TCGAGAGAGA TAGATTTGTA GAGAGAGACT GGTGATTTCA GCGTGTCCTC TCCAAATGAA ATGAACTTCC TTATATAGAG GAAGGTCTTG CGAAGGATAG TGGGATTGTG CGTCATCCCT TACGTCAGTG GAGATATCAC ATCAATCCAC TTGCTTTGAA GACGTGGTTG GAACGTCTTC TTTTTCCACG ATGCTCCTCG TGGGTGGGGG TCCATCTTTG GGACCACTGT CGGCAGAGGC ATCTTGAACG ATAGCCTTTC CTTTATCGCA ATGATGGCAT TTGTAGGTGC CACCTTCCTT TTCTACTGTC CTTTTGATGA AGTGACAGAT AGCTGGGCAA TGGAATCCGA GGAGGTTTCC CGATATTACC CTTTGTTGAA AAGTCTCAAT AGCCCTTTGG TCTTCTGAGA CTGTATCTTT GATATTCTTG GAGTAGACGA GAGTGTCGTG CTCCACCATG TTATCACATC AATCCACTTG CTTTGAAGAC GTGGTTGGAA CGTCTTCTTT TTCCACGATG CTCCTCGTGG GTGGGGGTCC ATCTTTGGGA CCACTGTCGG CAGAGGCATC TTGAACGATA GCCTTTCCTT TATCGCAATG ATGGCATTTG TAGGTGCCAC CTTCCTTTTC TACTGTCCTT TTGATGAAGT GACAGATAGC TGGGCAATGG AATCCGAGGA GGTTTCCCGA TATTACCCTT TGTTGAAAAG TCTCAATAGC CCTTTGGTCT TCTGAGACTG TATCTTTGAT ATTCTTGGAG TAGACGAGAG TGTCGTGCTC CACCATGTTG GCAAGCTGCT CTAGCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG
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