pUT-mini-Tn5-Gm
$128.00
In stock
SKU
PL69599
100μg liquid plasmid(Instantly):$163
| name | pUT-mini-Tn5-Gm | another name | apUT-mini-Tn5-Gm |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene editing | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | R6K | |
| Competence | S17-1λpir | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCCTTTTT GTCCGGTGTT GGGTTGAAGG TGAAGCCGGT CGGGGCCGCA GCGGGGGCCG GCTTTTCAGC CTTGCCCCCC TGCTTCGGCC GCCGTGGCTC CGGCGTCTTG GGTGCCGGCG CGGGTTCCGC AGCCTTGGCC TGCGGTGCGG GCACATCGGC GGGCTTGGCC TTGATGTGCC GCCTGGCGTG CGAGCGGAAC GTCTCGTAGG AGAACTTGAC CTTCCCCGTT TCCCGCATGT GCTCCCAAAT GGTGACGAGC GCATAGCCGG ACGCTAACGC CGCCTCGACA TCCGCCCTCA CCGCCAGGAA CGCAACCGCA GCCTCATCAC GCCGGCGCTT CTTGGCCGCG CGGGATTCAA CCCACTCGGC CAGCTCGTCG GTGTAGCTCT TTGGCATCGT CTCTCGCCTG TCCCCTCAGT TCAGTAATTT CCTGCATTTG CCTGTTTCCA GTCGGTAGAT ATTCCACAAA ACAGCAGGGA AGCAGCGCTT TTCCGCTGCA TAACCCTGCT TCGGGGTCAT TATAGCGATT TTTTCGGTAT ATCCATCCTT TTTCGCACGA TATACAGGAT TTTGCCAAAG GGTTCGTGTA GACTTTCCTT GGTGTATCCA ACGGCGTCAG CCGGGCAGGA TAGGTGAAGT AGGCCCACCC GCGAGCGGGT GTTCCTTCTT CACTGTCCCT TATTCGCACC TGGCGGTGCT CAACGGGAAT CCTGCTCTGC GAGGCTGGCC GGCTACCGCC GGCGTAACAG ATGAGGGCAA GCGGATGGCT GATGAAACCA AGCCAACCAG GAAGGGCAGC CCACCTATCA AGGTGTACTG CCTTCCAGAC GAACGAAGAG CGATTGAGGA AAAGGCGGCG GCGGCCGGCA TGAGCCTGTC GGCCTACCTG CTGGCCGTCG GCCAGGGCTA CAAAATCACG GGCGTCGTGG ACTATGAGCA CGTCCGCGAG CTGGCCCGCA TCAATGGCGA CCTGGGCCGC CTGGGCGGCC TGCTGAAACT CTGGCTCACC GACGACCCGC GCACGGCGCG GTTCGGTGAT GCCACGATCC TCGCCCTGCT GGCGAAGATC GAAGAGAAGC AGGACGAGCT TGGCAAGGTC ATGATGGGCG TGGTCCGCCC GAGGGCAGAG CCATGACTTT TTTAGCCGCT AAAACGGCCG GGGGGTGCGC GTGATTGCCA AGCACGTCCC CATGCGCTCC ATCAAGAAGA GCGACTTCGC GGAGCTGGTG AAGTACATCA CCGACGAGCA AGGCAAGACC GAGCGCCTGG GTCACGTGCG CGTCACGAAC TGCGAGGCAA ACACCCTGCC CGCTGTCATG GCCGAGGTGA TGGCGACCCA GCACGGCAAC ACCCGTTCCG AGGCCGACAA GACCTATCAC CTGCTGGTTA GCTTCCGCGC GGGAGAGAAG CCCGACGCGG AGACGTTGCG CGCGATTGAG GACCGCATCT GCGCTGGGCT TGGCTTCGCC GAGCATCAGC GCGTCAGTGC CGTGCATCAC GACACCGACA ACCTGCACAT CCATATCGCC ATCAACAAGA TTCACCCGAC CCGAAACACC ATCCATGAGC CGTATCGGGC CTACCGCGCC CTCGCTGACC TCTGCGCGAC GCTCGAACGG GACTACGGGC TTGAGCGTGA CAATCACGAA ACGCGGCAGC GCGTTTCCGA GAACCGCGCG AACGACATGG AGCGGCACGC GGGCGTGGAA AGCCTGGTCG GCTGGATCCG GCCACGATGC GTCCGGCGTA GAGGATCTGA AGATCAGCAG TTCAACCTGT TGATAGTACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT AACGTACTAA GCTCTCATGT TTAACGAACT AAACCCTCAT GGCTAACGTA CTAAGCTCTC ATGGCTAACG TACTAAGCTC TCATGTTTCA CGTACTAAGC TCTCATGTTT GAACAATAAA ATTAATATAA ATCAGCAACT TAAATAGCCT CTAAGGTTTT AAGTTTTATA AGAAAAAAAA GAATATATAA GGCTTTTAAA GCTTTTAAGG TTTAACGGTT GTGGACAACA AGCCAGGGAT GTAACGCACT GAGAAGCCCT TAGAGCCTCT CAAAGCAATT TTGAGTGACA CAGGAACACT TAACGGCTGA CATGGGAATT CCACATGTGG AATTCCACAT GTGGAATTGT GAGCGGATAA CAATTTGTGG AATTCCCGGG AGAGCTCCTG ACTCTTATAC ACAAGTTCGG CCACGGCCTA GGCGGCCGCA GCTTGTCGAC GAATTCCCGG GGATCCGGTG ATTGATTGAG CAAGCTTTAT GCTTGTAAAC CGTTTTGTGA AAAAATTTTT AAAATAAAAA AGGGGACCTC TAGGGTCCCC AATTAATTAG TAATATAATC TATTAAAGGT CATTCAAAAG GTCATCCACC GGATCAATTC CCCTGCTCAC ACAGACTGGG TACCAAGCTC TCGGTAACAT CAAGGCCCGA TCCTTGGAGC CCTTGCCCTC CCGACGATGA TCGTGCCGTG ATCGAAATCC AGATCCTTGA CCCACAGTTG CAAACCCTCA CTGATCCGTC CCGTCCGGGT CGACCAAAGC GGCCATCGTG CCTCCCCACT CCTGCAGTTC GGGGGCATGG ATGCGCGGAT AGCCGCTGCT GGTTTCCTGG ATGCCGACGG ATTTGCACTG CCGGTAGAAC TCCGCGAGGT CGTCCAGCCT CAGGCAGCAG CTGAACCAAC TCGCGAGGGG ATCGAGCCCG GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTTAGGTGG CGGTACTTGG GTCGATATCA AAGTGCATCA CTTCTTCCCG TATGCCCAAC TTTGTATAGA GAGCCACTGC GGGATCGTCA CCGTAATCTG CTTGCACGTA GATCACATAA GCACCAAGCG CGTTGGCCTC ATGCTTGAGG AGATTGATGA GCGCGGTGGC AATGCCCTGC CTCCGGTGCT CGCCGGAGAC TGCGAGATCA TAGATATAGA TCTCACTACG CGGCTGCTCA AACCTGGGCA GAACGTAAGC CGCGAGAGCG CCAACAACCG CTTCTTGGTC GAAGGCAGCA AGCGCGATGA ATGTCTTACT ACGGAGCAAG TTCCCGAGGT AATCGGAGTC CGGCTGATGT TGGGAGTAGG TGGCTACGTC TCCGAACTCA CGACCGAAAA GATCAAGAGC AGCCCGCATG GATTTGACTT GGTCAGGGCC GAGCCTACAT GTGCGAATGA TGCCCATACT TGAGCCACCT AACTTTGTTT TAGGGCGACT GCCCTGCTGC GTAACATCGT TGCTGCTGCG TAACATGCGA AACGATCCTC ATCCTGTCTC TTGATCAGAT CTTGATCCCC TGCGCCATCA GATCCTTGGC GGCAAGAAAG CCATCCAGTT TACTTTGCAG GGCTTCCCAA CCTTACCAGA GGGCGCCCCA GCTGGCAATT CCGGTTCGCT TGCTGTCCAT AAAACCGCCC AGTCTAGCTA TCGTCATGTA AGCCCACTGC AAGCTACCTC TTTCTCTTTG CGCTTGCGTT TTCCCTTGTC CAGATAGCCC AGTAGCTGAC ATTCATCCGG GGTAGCACCG TTTCTGCGGA CTGGCTTTCT ACGTGTTCCG CTTCCTTTAG CAGCCCTTGC GCCCTGATGC TTGCGGCAGC GTGAAGCTCG CGCAGATCAG TTGGTTGATT TTGTCCACTA CGTGAAAGGC GAATCACCAA GGTAGTCGGC AAATAATGTC TAACAATTCG TTCAAGCCGA CGCCGCTTCG CGGCGCGGTT AACTCAAGCG TTAGATGCAC TAAGCACATA ATTGCTCACA GCCAAACTAT CAGGTCAAGT CGCTTTTATT ATTTTTAAGC GTGCATAATA AGCCCTACAC AAAATTGGGA GATATATCAT GAAAGGCGCT TTTCTTGTTA TCGCAATAGT TGGGCGAAGT AATCGCAACA TCCGCATTAA CAATCTAGCG AGGGCTTGGT AAGGTGATCC GGTGGATGAC CTTTTGAATG ACCTTTAATA GATTATATTA CTAATTAATT GGGGACCCTA GAGGTCCCCT TTTTTATTTT AAAAATTTTT TCACAAAACG GTTTACAAGC ATAAAGCTTG CTCAATCAAT CACCGGATCC CCGGGAATTC GGCCTAGGTG GCCAGATCTG ATCAAGAGAC AGAAACGCAA AAGAAAATGC CGATATTAAT ACGACTCACT ATAGGGGAAT TGTGAGCGGA TAACAATTCC CCTCTAGAAA TAATTTTGTT AACTTTAAGA AGGAGATATA CATATGATTA CCAGTGCACT GCATCGTGCG GCGGATTGGG CGAAAAGCGT GTTTTCTAGT GCTGCGCTGG GTGATCCGCG TCGTACCGCG CGTCTGGTGA ATGTTGCGGC GCAACTGGCC AAATATAGCG GCAAAAGCAT TACCATTAGC AGCGAAGGCA GCAAAGCCAT GCAGGAAGGC GCGTATCGTT TTATTCGTAA TCCGAACGTG AGCGCGGAAG CGATTCGTAA AGCGGGTGCC ATGCAGACCG TGAAACTGGC CCAGGAATTT CCGGAACTGC TGGCAATTGA AGATACCACC TCTCTGAGCT ATCGTCATCA GGTGGCGGAA GAACTGGGCA AACTGGGTAG CATTCAGGAT AAAAGCCGTG GTTGGTGGGT GCATAGCGTG CTGCTGCTGG AAGCGACCAC CTTTCGTACC GTGGGCCTGC TGCATCAAGA ATGGTGGATG CGTCCGGATG ATCCGGCGGA TGCGGATGAA AAAGAAAGCG GCAAATGGCT GGCCGCTGCT GCAACTTCGC GTCTGAGAAT GGGCAGCATG ATGAGCAACG TGATTGCGGT GTGCGATCGT GAAGCGGATA TTCATGCGTA TCTGCAAGAT AAACTGGCCC ATAACGAACG TTTTGTGGTG CGTAGCAAAC ATCCGCGTAA AGATGTGGAA AGCGGCCTGT ATCTGTATGA TCACCTGAAA AACCAGCCGG AACTGGGCGG CTATCAGATT AGCATTCCGC AGAAAGGCGT GGTGGATAAA CGTGGCAAAC GTAAAAACCG TCCGGCGCGT AAAGCGAGCC TGAGCCTGCG TAGCGGCCGT ATTACCCTGA AACAGGGCAA CATTACCCTG AACGCGGTGC TGGCCGAAGA AATTAATCCG CCGAAAGGCG AAACCCCGCT GAAATGGCTG CTGCTGACCA GCGAGCCGGT GGAAAGTCTG GCCCAAGCGC TGCGTGTGAT TGATATTTAT ACCCATCGTT GGCGCATTGA AGAATTTCAC AAAGCGTGGA AAACGGGTGC GGGTGCGGAA CGTCAGCGTA TGGAAGAACC GGATAACCTG GAACGTATGG TGAGCATTCT GAGCTTTGTG GCGGTGCGTC TGCTGCAACT GCGTGAATCT TTTACTCCGC CGCAAGCACT GCGTGCGCAG GGCCTGCTGA AAGAAGCGGA ACACGTTGAA AGCCAGAGCG CGGAAACCGT GCTGACCCCG GATGAATGCC AACTGCTGGG CTATCTGGAT AAAGGCAAAC GCAAACGCAA AGAAAAAGCG GGCAGCCTGC AATGGGCGTA TATGGCGATT GCGCGTCTGG GCGGCTTTAT GGATAGCAAA CGTACCGGCA TTGCGAGCTG GGGTGCGCTG TGGGAAGGTT GGGAAGCGCT GCAAAGCAAA CTGGATGGCT TTCTGGCCGC GAAAGACCTG ATGGCGCAGG GCATTAAAAT CTAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGACGCGTAA AACAGCCAGC GCTGGCGCGA TTTAGCCCCG ACATAGCCCC ACTGTTCGTC CATTTCCGCG CAGACGATGA CGTCACTGCC CGGCTGTATG CGCGAGGTTA CCGACTGCGG CCTGAGTTTT TTAAGTGACG TAAAATCGTG TTGAGGCCAA CGCCCATAAT GCGGGCTGTT GCCCGGCATC CAACGCCATT CATGGCCATA TCAATGATTT TCTGGTGCGT ACCGGGTTGA GAAGCGGTGT AAGTGAACTG CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCA
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