pAN8-1
$157.00
In stock
SKU
PL69287
100μg liquid plasmid(Instantly):$178
| name | pAN8-1 | another name | apAN8-1 |
| Insert Size (bp) | Plasmid host | filiformFungus | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Zeo | Fluorescent labeling | |
| Promoter | gpdA | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAATTCCCTT GTATCTCTAC ACACAGGCTC AAATCAATAA GAAGAACGGT TCGTCTTTTT CGTTTATATC TTGCATCGTC CCAAAGCTAT TGGCGGGATA TTCTGTTTGC AGTTGGCTGA CTTGAAGTAA TCTCTGCAGA TCTTTCGACA CTGAAATACG TCGAGCCTGC TCCGCTTGGA AGCGGCGAGG AGCCTCGTCC TGTCACAACT ACCAACATGG AGTACGATAA GGGCCAGTTC CGCCAGCTCA TTAAGAGCCA GTTCATGGGC GTTGGCATGA TGGCCGTCAT GCATCTGTAC TTCAAGTACA CCAACGCTCT TCTGATCCAG TCGATCATCC GCTGAAGGCG CTTTCGAATC TGGTTAAGAT CCACGTCTTC GGGAAGCCAG CGACTGGTGA CCTCCAGCGT CCCTTTAAGG CTGCCAACAG CTTTCTCAGC CAGGGCCAGC CCAAGACCGA CAAGGCCTCC CTCCAGAACG CCGAGAAGAA CTGGAGGGGT GGTGTCAAGG AGGAGTAAGC TCCTTATTGA AGTCGGAGGA CGGAGCGGTG TCAAGAGGAT ATTCTTCGAC TCTGTATTAT AGATAAGATG ATGAGGAATT GGAGGTAGCA TAGCTTCATT TGGATTTGCT TTCCAGGCTG AGACTCTAGC TTGGAGCATA GAGGGTCCTT TGGCTTTCAA TATTCTCAAG TATCTCGAGT TTGAACTTAT TCCCTGTGAA CCTTTTATTC ACCAATGAGC ATTGGAATGA ACATGAATCT GAGGACTGCA ATCGCCATGA GGTTTTCGAA ATACATCCGG ATGTCGAAGG CTTGGGGCAC CTGCGTTGGT TGAATTTAGA ACGTGGCACT ATTGATCATC CGATAGCTCT GCAAAGGGCG TTGCACAATG CAAGTCAAAC GTTGCTAGCA GTTCCAGGTG GAATGTTATG ATGAGCATTG TATTAAATCA GGAGATATAG CATGATCTCT AGTTAGCTCA CCACAAAAGT CAGACGGCGT AACCAAAAGT CACACAACAC AAGCTGTAAG GATTTCGGCA CGGCTACGGA AGACGGAGAA GCCACCTTCA GTGGACTCGA GTACCATTTA ATTCTATTTG TGTTTGATCG AGACCTAATA CAGCCCCTAC AACGACCATC AAAGTCGTAT AGCTACCAGT GAGGAAGTGG ACTCAAATCG ACTTCAGCAA CATCTCCTGG ATAAACTTTA AGCCTAAACT ATACAGAATA AGATAGGTGG AGAGCTTATA CCGAGCTCCC AAATCTGTCC AGATCATGGT TGACCGGTGC CTGGATCTTC CTATAGAATC ATCCTTATTC GTTGACCTAG CTGATTCTGG AGTGACCCAG AGGGTCATGA CTTGAGCCTA AAATCCGCCG CCTCCACCAT TTGTAGAAAA ATGTGACGAA CTCGTGAGCT CTGTACAGTG ACCGGTGACT CTTTCTGGCA TGCGGAGAGA CGGACGGACG CAGAGAGAAG GGCTGAGTAA TAAGCCACTG GCCAGACAGC TCTGGCGGCT CTGAGGTGCA GTGGATGATT ATTAATCCGG GACCGGCCGC CCCTCCGCCC CGAAGTGGAA AGGCTGGTGT GCCCCTCGTT GACCAAGAAT CTATTGCATC ATCGGAGAAT ATGGAGCTTC ATCGAATCAC CGGCAGTAAG CGAAGGAGAA TGTGAAGCCA GGGGTGTATA GCCGTCGGCG AAATAGCATG CCATTAACCT AGGTACAGAA GTCCAATTGC TTCCGATCTG GTAAAAGATT CACGAGATAG TACCTTCTCC GAAGTAGGTA GAGCGAGTAC CCGGCGCGTA AGCTCCCTAA TTGGCCCATC CGGCATCTGT AGGGCGTCCA AATATCGTGC CTCTCCTGCT TTGCCCGGTG TATGAAACCG GAAAGGCCGC TCAGGAGCTG GCCAGCGGCG CAGACCGGGA ACACAAGCTG GCAGTCGACC CATCCGGTGC TCTGCACTCG ACCTGCTGAG GTCCCTCAGT CCCTGGTAGG CAGCTTTGCC CCGTCTGTCC GCCCGGTGTG TCGGCGGGGT TGACAAGGTC GTTGCGTCAG TCCAACATTT GTTGCCATAT TTTCCTGCTC TCCCCACCAG CTGCTCTTTT CTTTTCTCTT TCTTTTCCCA TCTTCAGTAT ATTCATCTTC CCATCCAAGA ACCTTTATTT CCCCTAAGTA AGTACTTTGC TACATCCATA CTCCATCCTT CCCATCCCTT ATTCCTTTGA ACCTTTCAGT TCGAGCTTTC CCACTTCATC GCAGCTTGAC TAACAGCTAC CCCGCTTGAG CAGACATCAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACCGA CGCCGACCAA CACCGCCGGT CCGACGGCGG CCCACGGGTC CCAGGAGCTT GAGATCCACT TAACGTTACT GAAATCATCA AACAGCTTGA CGAATCTGGA TATAAGATCG TTGGTGTCGA TGTCAGCTCC GGAGTTGAGA CAAATGGTGT TCAGGATCTC GATAAGATAC GTTCATTTGT CCAAGCAGCA AAGAGTGCCT TCTAGTGATT TAATAGCTCC ATGTCAACAA GAATAAAACG CGTTTTCGGG TTTACCTCTT CCAGATACAG CTCATCTGCA ATGCATTAAT GCATTGACTG CAACCTAGTA ACGCCTTnCA GGCTCCGGCG AAGAGAAGAA TAGCTTAGCA GAGCTATTTT CATTTTCGGG AGACGAGATC AAGCAGATCA ACGGTCGTCA AGAGACCTAC GAGACTGAGG AATCCGCTCT TGGCTCCACG CGACTATATA TTTGTCTCTA ATTGTACTTT GACATGCTCC TCTTCTTTAC TCTGATAGCT TGACTATGAA AATTCCGTCA CCAGCnCCTG GGTTCGCAAA GATAATTGCA TGTTTCTTCC TTGAACTCTC AAGCCTACAG GACACACATT CATCGTAGGT ATAAACCTCG AAATCAnTTC CTACTAAGAT GGTATACAAT AGTAACCATG CATGGTTGCC TAGTGAATGC TCCGTAACAC CCAATACGCC GGCCGAAACT TTTTTACAAC TCTCCTATGA GTCGTTTACC CAGAATGCAC AGGTACACTT GTTTAGAGGT AATCCTTCTT TCTAGAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTAC
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