UCOE Mu-H
$171.00
In stock
SKU
PL69059
100μg liquid plasmid(Instantly):$192
| name | UCOE Mu-H | another name | aUCOE Mu-H |
| Insert Size (bp) | Plasmid host | Mammary cell | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Hyg | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GCGGCCGCCA GTGGAACGAA AACTCACGTT GTAAAACGAC GGCCAGTGAG CGCGCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAAGTTTAT CTAGGGATAA CAGGGTAATG ATAAACATTT CGGATAACAA TTTCACACAG GAAACAGCTA TGACCATGAT TACGCCAAGC TTGGTACCGA GCTCGGATCC ACTAGTAACG GCCGCCAGTG TGCTGGAATT CGCCCTTTAC CACTAAGCCA TCTCTCCAGC CCTGAGTCAT GGTTTTAGTG TGAGAGGCAT CATTGAATTT TCTGAGCACG GCCATCAGGG TAGCTGGCAC AGGTCTTCAG ATACAAGGAG ATAGTTATAA GAAGGCAGCC ATGGCTGTGG TGCACTAGAA ATGGAGAAAC AGCTTCATCA GGTGACAGAC CAGTCTGACT CTGTCCCATG ATTAGAAGCC ATCTTGTTAC AAGGTCAAAA TAAGTTCATT CCTGTTTTCT GTAACACTTG GGTTTGATCC TGTCGTCAAC CCATTTTCTG GAATTTGACA TGTTCCATAC TCCATTATAC CCTGACTTCC ACCCTGATAA GATGTTCTGC CAAGTTCCTG TGTAGCCAAC ATTCCCCTGG AAATCTCTCT TCCCTTGGAA ACCACCTAGT CTTAGAAATT TTGAGTTATA TAAATTCCAC TTCTATGTTT GATGCTATTC TTTAAAACTC CACTTTAGGG AGATAGCCCT GTCTGATAGA AAATAAAACT TGCTTAATTT GTCTAAAAGA TTTTAAGTAA TAGTTTTTAC TTTTGTTCCG TGGGATTAGT ACAGGGTGAA ACAGACTCCC GTGTTTCCAG TGTGAAGTGA GCCACACACT GCAGTACAAG TTATATCAGC AGGTTCTGCC TCTGCGCAAT GAACTTTTGC TTGTGTGGAC ATCAGGGTCT GTGTGAAGGG AAGGTCCTAT GGCCTAGTTT TATACTATTC AACAGTCTGT CCCCGAAGCC CTGGTGCTTT ATTATTTTGA CAAGCCCCTG CTGCTGGTAT TCCACCCTGC TGCGAGTCAA AAAAGTTCCT GTCTCGGAAA AACAAAACAA AACAAAACAA CCAAAAAATA AATTTTTTTT TCCCACAGGT TCTAGTGGAG GTGCTCACTA CCAGAAATCC TACAAATAAG CCCATCTCAT GGATCAGGGT TTACCTTTGT AATAATATTA AATCTGTGTG CATGTGCGCA CGCATGTGTT TTATGCTTGC ATATATGTAT ACGCAGCCAT GGTTTTCTAC TGTCCCACTC ACTCTGTAAC TTACTGAGCC ATCCAGCTGG TCCTCTAAAT ACATTTCAAT GAAAGTTTTC ATTAGCGTGA ACGTGAAGGT GGTAAAATCT GTTAGTGTGT GCTTATGCCT GTGGTTTGCA CCTCTAGTCT GAAGGTTGCT CTTTTCAAAT TTTTTATTTA TTTACGTTTT TACTTCTGAG TCAGAAACTC ATAAAGGCCA TGGCCTCGAA TTCGCTATGT AGTCAACGAT GACCTTAAAC TTGTGACCCT CTACTTCGTT AGTGCTGGAA CCCCAAGCTT GCTGAGTACA GAGCACTTTC AGACCGGAAC TAGATGTCTA CTTCCTGTTC CGCCTACATT ACAGGTTGCT AGGTTACACC CCCCCTACGC CGTTTTAGAC GCAAAACTTC ATTTCCCATG CAAAACTTCA TTTCCCATGA ACACTTGCAA GGGTCGCCGC GCTGCGCGGC GTCATTGCTC CCGCCCTATA TACCTACTTC CGCCCGCGAG CCACTTCCTT TCCTTTCAGC GGCGCGCGGC TGCAAGATGG CGGTGCAGAT TTCCAAGAAG AGGAAGGTAA GCGTCTGGGC CCGGTTCGGG AGTCCGCCGC GGGTTCTACA AGTGCCAGGG AGGCCTGTGG CTCCGTAATC AGTCCTGTGG AGCGTCTGGG GCCGCCTGCC GTCTCTTCGA GCCTCGGATG GCCGTAGATT GTGTATTGGG CCGGAGCCGG GCGAGTGCTG TGTGCCTGGG CAAGGGAGGG ACAAACTCCT CGAGTTCTGG ACCGACTCGA ACACCGGGCG CCTCCAGTTC CGGACTAGAC ACCTTTGAGC GTTTCTTGGT CTCCATAATA GTAATCCTGT GGCACAGTTA GAGGGCGTGT GCCATCAGAT CTAGTCCAGT CTCTTTAGTA AGTGAAGTTT AGCAGTCCCT TCTCTTAGTC GCGTGATCCT GCAAGTGGCC ATAGTTGAAA GCCTACTTAC TGACTGCTGC CGTGTTCACT CGGGACCCGG AGCTGCAGCG TCCCTGTGGT TATCATTTCA TGGGGGAAAA GTGTGCAGGT TGCCAGGTTT AGAAATAGAT GGTCTGTCGT TTGTGCTTAT GCACACAGAT GATAAACCTG TTTTGAGTCA GGATTCCTCT CCTATCCGAG GTACAACTTA CAGTCCCAGC TGTACATGTG CTACTTGGAG ACAGATTTTT CTTTGTCTCT TGGGTGTAGA TTATGCCGTA GAGCCCTTCG ATGAAGAGGT GATGACGAGT CTGAGTAGGA AGTGTTGTCT TTGTCCAAGA TGCCTCACTA TGCTGCGTTC TGTGGCACAG CTGAAAGCAC TGTGGTCAAA AGAAACTTCC TAAAGATGAC CAAGAGGCAT TTGTCTGAGA AGGGTTGCTG CTTTTCTGTA GGGCCATTGG GCTTGCTCTG ACTAACCCTG TCTTCACCTC AGAGGTAACT TGTTTCCTTT GGTTCAGTTT GTAGCTGATG GCATCTTCAA AGCTGAGCTG AATGAATTTC TCACTCGGGA GCTGGCTGAA GATGGCTACT CTGGAGTTGA AGTCCGAGTT ACACCAACCA GGACAGAAAT CATTATTTTA GCCACCAGGT AGAAATACCA TTGATTGTCA CCTGTAAATA TTGTGTGTAC TGAGATGCTG TGTAAACTTG GGCCAACCAA GCAGTAAATC TGGCCTCAGT GGGTGTAACT GCTTTGTTAG AACTGCATTT GGGAAGAACT TACCTTCCAT TTAACCTGTG TGCTGGCGTT GTGGTGGGCG GCAGGTGGGA TCTTGAGTAA ATGGTTGCGC TTCCCCTCTA CAGGACACAG AATGTTCTTG GGGAGAAGGG TCGTCGGATC AGAGAGTTGA CCGCAGTTGT CCAGAAGCGC TTTGGCTTCC CTGAAGGCAG CGTAGAGGTG AGTTCCTCTG CTTTATCTCC CGGGGGTTTT AGACTGAGTT GGGATGTGGC TTCTGCTATA GAATTGTACT TCTGAAAACC TGACATGGCC AGTGACAGTC ACAGGTACTT GATGCTAAGG GCGAATTCTG CAGATGGGCC GTACGAATTT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGAAT TCGAGCTCCC TGCAGGTTAG TTAAGTTAAC GGCCGGCCGC TAGCGCGATC GCGAGCTCGG TACCCGGGGA TCCTCTAGAG TCGACCTGCA GGCATGCAAG CTGGCCGCGA CTCTAGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAAAGCTAT CCATCACACT GGCGGCCGAA ATCTAGAAGC TTATCGATAT GGCGCGCCAA TTGTTAGGCC GCATTCGCCA ATCAAGTAAG CTAAGATCTG GCCCAATGGG CCGTACGAAT TCTACCGGGT AGGGGAGGCG CTTTTCCCAA GGCAGTCTGG AGCATGCGCT TTAGCAGCCC CGCTGGGCAC TTGGCGCTAC ACAAGTGGCC TCTGGCCTCG CACACATTCC ACATCCACCG GCCGGTAGGC GCCAACCGGC TCCGTTCTTT GGTGGCCCCT TCGCGCCACC TTCTACTCCT CCCCTAGTCA GGAAGTTCCC CCCCGCCCCG CAGCTCGCGT CGTGCAGGAC GTGACAAATG GAAGTAGCAC GTCTCACTAG TCTCGTGCAG ATGGACAGCA CCGCTGAGCA ATGGAAGCGG GTAGGCCTTT GGGGCAGCGG CCAATAGCAG CTTTGCTCCT TCGCTTTCTG GGCTCAGAGG CTGGGAAGGG GTGGGTCCGG GGGCGGGCTC AGGGGCGGGC TCAGGGGCGG GGCGGGCGCC CGAAGGTCCT CCGGAGGCCC GGCATTCTGC ACGCTTCAAA AGCGCACGTC TGCCGCGCTG TTCTCCTCTT CCTCATCTCC GGGCCTTTCG ACGGTACCGC GGGCCCGGGG GATCCACTAG TACTATGAAA AAGCCTGAAC TCACCGCGAC GTCTGTCGAG AAGTTTCTGA TCGAAAAGTT CGACAGCGTC TCCGACCTGA TGCAGCTCTC GGAGGGCGAA GAATCTCGTG CTTTCAGCTT CGATGTAGGA GGGCGTGGAT ATGTCCTGCG GGTAAATAGC TGCGCCGATG GTTTCTACAA AGATCGTTAT GTTTATCGGC ACTTTGCATC GGCCGCGCTC CCGATTCCGG AAGTGCTTGA CATTGGGGAG TTCAGCGAGA GCCTGACCTA TTGCATCTCC CGCCGTGCAC AGGGTGTCAC GTTGCAAGAC CTGCCTGAAA CCGAACTGCC CGCTGTTCTC CAGCCGGTCG CGGAGGCCAT GGATGCGATC GCTGCGGCCG ATCTTAGCCA GACGAGCGGG TTCGGCCCAT TCGGACCGCA AGGAATCGGT CAATACACTA CATGGCGTGA TTTCATATGC GCGATTGCTG ATCCCCATGT GTATCACTGG CAAACTGTGA TGGACGACAC CGTCAGTGCG TCCGTCGCGC AGGCTCTCGA TGAGCTGATG CTTTGGGCCG AGGACTGCCC CGAAGTCCGG CACCTCGTGC ATGCGGATTT CGGCTCCAAC AATGTCCTGA CGGACAATGG CCGCATAACA GCGGTCATTG ACTGGAGCGA GGCGATGTTC GGGGATTCCC AATACGAGGT CGCCAACATC CTCTTCTGGA GGCCGTGGTT GGCTTGTATG GAGCAGCAGA CGCGCTACTT CGAGCGGAGG CATCCGGAGC TTGCAGGATC GCCGCGCCTC CGGGCGTATA TGCTCCGCAT TGGTCTTGAC CAACTCTATC AGAGCTTGGT TGACGGCAAT TTCGATGATG CAGCTTGGGC GCAGGGTCGA TGCGACGCAA TCGTCCGATC CGGAGCCGGG ACTGTCGGGC GTACACAAAT CGCCCGCAGA AGCGCGGCCG TCTGGACCGA TGGCTGTGTA GAAGTACTCG CCGATAGTGG AAACCGACGC CCCAGCACTC GTCCGAGGGC AAAGGAATAG AGTAGATGCC GACCGGGAAC CAGTTAACGT CGACCTCGAG GGGGGGCCCG GTACCAGCTT TTGTTCCCTT TAGTGAGGGT TAATTTCGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CG
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