pSTV28
$84.00
In stock
SKU
PL68213
100μg liquid plasmid(Instantly):$120
| name | pSTV28 | another name | apSTV28 |
| Insert Size (bp) | Plasmid host | Escherichia coli | |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Empty vector | Prokaryotic resistance | Chl |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | p15A | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | lac-P | Copy number | low |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
aaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgtttgacag cttatcatcg ataagctcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggaa cgccatgagc ggcctcattt cttattctga gttacaacag tccgcaccgc tgtccggtag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct aaccgttttt atcaggctct gggaggcaga ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacg
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