pCMV-KREMEN2(human)-2-3×FLAG-Neo
$70.00
In stock
SKU
PL67925
NM_172229.3;KRM2
100μg liquid plasmid(Instantly):$91
| name | pCMV-KREMEN2(human)-2-3×FLAG-Neo | another name | apCMV-KREMEN2(human)-2-3×FLAG-Neo |
| Insert Size (bp) | 1389bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GACTAGATTG CCTTCGAGTT TTTTTTTTTT TTTTTTACCA AAGTAGATCC TTTATTCAAA ATCTGTGGGG GGTTTTTTGA CTGTTTTTGC TGACCTGTGA CCTCTCACTG AGGACGGCAG TCCAGGACCA CCCCCAGAGG GGTTCAAAGA CCTCCGAAAC CAGAGACCAC CCCCACATAC AGGTCCATGG AGAGGGCCTG GACCATCTGG ACCCCCACGG TCAGGCCAAG TCCCAGATAA TAGCACCAGA TGTTTCCTGT CCGACTTTTG GTCTCCCCAA GGCGACCAGA GGCCGAGCTG CCCCTACACA GGCGCAAGGC CGAGGCAGGG CGCAGCACAG CATCTCAGGT GTCCATCTCG CCGGCGGCGG GCCCAGCGGA CCCTCGGGGC CCAGAGTCAG AGAGCGGAGA TGAGCGAGCG CAGGGAGCTC TGGCTGGAGG CACTCAGAGG CCGGTAGCCC GCGGCAGAAC CCTCAGCCTG GGGGTCCCCG GGGGAGCAGG GCAAGGCCAC CCCTCGGGGC TGTTGGTACC ACACAGCCCA GCTTCTCCTG GGGCCCCTGG AAGCCCCCAG CGCCGGGGGC CCTTTTCCCG GAGCCAGCAG ACAGCTCCTA GAGAGGGAGC AGACATCAGC CGAGCCGGTC CAATCCCGCT GCAGCGTCCT GCTCTACCTG CGATCCCGCG CGCGATCCTG TGCGTGGACC CAAAGCCTCG CTCCCTTAAG AACCCAGGAG TTCCTTCCTA GCTCTGTTCC CTTCTGGGGC TCCAGCTCCC CACCACCGGT CCGCCCTCAG GCCCTGCCCC AGCGCCCCGC ACCGTCGGCG CAGCGGACGC AGCAGCCCCA GGAGCAGCAG CAGCAGCACC GAGACAGCCG TCACCGTCGA GAAGACCCGG GCTGCGGGCA CACGGCTGCG GTGAGGATGG GCGCGAGGGG GCGGCTCCCT GACTCACTCC CGTCCCGCGC GCCCGCCTCA CCCCCAATCG CGGCCGGCGG AGCCCCAGGC CTGGGGCTGC AGCTCACGTT GGCCCCGTCG AGGGGCGCCG CGGGGGTCTG GGCCGAGCCC TCGGGGGCCT CTGGGTCCTC AGCGGCGTCC TGCAGCCCGC GGTAGGTGAG CGCGAAGCCT TGCGCGTGGC CGCGCGCGTC GCTTCGGAAG GTGAGCAGCA GCGCGGCAGT GCCCAGGCGC AGCGGCCCGG ACGGCGGTGG GCGGGCGCCA TCGAAGGCGC GGAGCAGGCT GCCCGAAGCC GCGTCGCGCA GCTCCAGCCG GTCGCGCGGG TCGGCCAGCT CGAAGAGGCG GAAGGTGAGC TCCAGCGCGG CGCCTGGCGG GCCCAGGGCC CAGCTGCAGT TCCGGTCCGG CCCGTACTCG TCCGGGAAGT CCGGGGAGTA GATGACGCCC TGAGGCGCTG TCCAGTTCCC CTGGCAGGAG CCCACCGACA CTTCATAGAC GCCCAGCCGC CCATCGCCGC CACACAGCTG TCCAGGGTGG CCGAAACAGA TCTGGTCACA GTCGGTGGCG GGGGCCAGGC GTCCCCGGGC CAGGTCGCTT TCAGAGCCAC AGAAGCAGGC GTAACCGGCC TCCACGCCCG CCAGCTGGTA CCCCTTCATG CGGCAGAAGC GTAGGCACAC CTGGACCGTG AGCTTCGTGG AGGTGCCGCT GGGGCCGCTG AGGGCTGGGG GTGCCCCTGA GTCCACAAAG CATCCCAGGT AGCCTGGCAT GTGACAGGAG GGGATGTCGC AGTAGCGCCA GTAGATGCCC TCCTCTGTCT CAGCCACGTA GCACCACGGC TGCACGTCAC CGTCTGGGTT ACGGCAGAAG TTGTGCGCGC CCAGCCCCCA GCGGCCGTGG GGGTCGCTGG CGCTGCTGTA GCTGTGTTGC TGCGTCTGGT CCCAGAAGAG GCACGGGCGG CCCGCCCCGC GCGGGCCAGT GCGGTTCTGG TGGCCGCGGT AGTCAGCCCC ATTCACCTGG AAGCATTCGG ACAGGCCTGG ACTGTGCAGG CTCCCAGCCG AGGCCCCACG CGGCTGCAGC AGCGGGAGGA AGAGGAGAAA GAGGAAGCCC TGCAGGGCTT GTGTCCCCAT CGCTCTCAAC CAAGGATAGG TCACCCTCGC CCGGACACGG CCCGGGCTGC CCCCGGGGTC GCTTCGGGGT CTCCCAGGAG ACCTAGGGGG CGTTGTCCTC TGACAGTCTC CCCTCGTCCG ACTCTGAGCC GCGCTTTCCG TCTGGGGTGG GTAAATCTCT CCTGGGGGTC TTTGCCTCCC AGGGCCTCTG CCCCTAGAGG CGTCTCTCTG TCCCTGTCCC TACCCGGGTC TCTCCGGGGG GAATCTGGCC GGGGGCGTTC TCAGTCTCTG TCTCAGCGAG CTCTGGGCGG TGCCTCAGCC TCTACCCTCG TGCCGAATCG CC
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