pNL4-3-Luc-R-E-
$113.00
In stock
SKU
PL67546
100μg liquid plasmid(Instantly):$134
| name | pNL4-3-Luc-R-E- | another name | apNL4-3-Luc-R-E- |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | Fluc | |
| Promoter | Replicon | pUC | |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGCTGGACTG GCCTAGATTT TGTATACACA CATTTCAGGT GAAGGTCTAA AAAAAACTGG AGCATATAAA GAAAAATAGA ATATATGTGT AATTCTGGAA TAAGGAAGGA CTGTGTTTCC AAAATAGAAA ATAAATAAAT AAATAAAGGA AAAGATTGGA ATATCTGATT GATTTATTTA TTTTATTTTT GAGACAGAGT CTTACTCTGT CGCCCAGGCT GGAGTGCAGT GCTGCGATCT CAGCTTACTG CAATCTCTGC CTCCCCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC CAATACGCAA ACCGCCTCTC CCCGCGCGTT GGCCGATTCA TTAATGCAGC GACTGGTGTC AGAAGCGGGC AGTCTTTGAA CCACCATTGC AAAATTATAA CTCAGACAGT GAAAGAGATC TGACCTAACC AATTCCATCG TGCTTCTAAC CTCCAAGCTC TCCTTGTTCA TTCCTGGGTG TAGGCCGAAC TAAGTTTAGG AGGAACTTAG TTTATAGTTT GAAACAAAGA CAGTAACAGC CCTTTCCCGA AACAAACCCC CTTTTTGCCT GGGGACTAGA CTGCCTTTGT AGGACTAACA AATTAGCCAA AAGATTAGAA ATTATGGTTT AGGGCCAGGT GCAGTGACTG GCCCCGGTTG TCCCAGCACT TTGGGAGGCT GAGGCTGGAT CACTTGAGCC CAGGAGTTCG AGACCAGCCA GGGCCACACG GCAAAACGCT GCCTCTACCA AAAATACAAA AATTAACTGG GGCGTCGCTT GTAGTCTCAG CTACCAGGCA GGCTGAGGTG GGAGGATCGC TTGAGCTTAC GACGTTGAGG CTGCAGTGAG CTGTGATTGC ACCACTGCAC TCCAGCCTAG GTGACAGAGT GAGACCCTGT CTCAAAAATA AAAAAAAAAA AACAAAAAAG AAATTATGGT TTAGGAGTCA TGCAGCTACA GGCTACAAGA TTCTGACCTT CCCCAAATTG TTCCTGGGGT AACATCACTA TTGTAAAACC TAAGATCAAT CAGTGCTTGA GATATTTTGC AGACCCTGCA CTCCATGGAT CAGCTGGCAC CCCCGGATTG ATAAACTGGC TCATCCGATC TTGTGGCCCC CACCCAGGAA CTGACTCGGC GCAAAAGGAC ACCTTCGACT CCCTACGCTT TCATCTCCAA CTCACTGGCC CCCTACACAC TAAGTTATCC TTAAAAACTC TGATCCCCGA ATGCTTGGGG AGACTGATTT GAGTCATAAT AGAACTCCGT TCTCCCGCAC AGCCGGCTCT GTGTGACTTA CTCTTTCTCT ATCCTAATTC CCGTCTTGGT GAATCGGCTC TGCCTGGGCA GCAGGCGGGG TGGACCTGTT GGGTGTTTAT GTCTTGGGTG TTTATGTCTT GGGGACTGAG CCCTCACCCT GTGGTCTGAC CTCTCTCCAG GCAGGCGGTG TTGGAACTGA ACCGAAGGAC ACCCGCCGAG TCTGCGGCAG AACTGACTGC TCATGTGCTG GTGAAAACCT CCCTCTCCCA CCACATTTGA TCACAGAAGT CTTCCTCTGT GCTGATGATT CTTGTGGTAT CAGAGTAGAG GAAAAACAGT TTCAGTTTTC CAGAAATACC TGTGAATAAA ATACACATAA AATAAAATCT ACTAAAAATA CACAGAGAAG GGGTTTCTCC ATGTTGGTCA GGCTGGTCTG GAACTCCCAA ACTCAGGTGA TCCACCTGCC TCGGCCTCCC AAAGGGCCGG GCGCAGGTGT GAGCCACTGC GCCCAGCCCA AATATATATA TTTTTATGAC CTCCATGTAT AAATATATTT AATACCCTTA ACTTATTATT ATTATTTTAG ACAGTCTTGT TTTCTTGCCC AGGCTGGAAT GCAGTGGCGC GATCTTGGCT CACTGCAACC TCTACCTCCT GGGTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAAAGC AGCTGCTTAT ATGCAGCATC TGAGGGCTCG CCACTCCCCA GTCCCGCCCA GGCCACGCCT CCCTGGAAAG TCCCCAGCGG AAAGTCCCTT GTAGCAAGCT CGATGTCAGC AGTTCTTGAA GTACTCCGGA TGCAGCTCTC GGGCCACGTG ATGAAATGCT AGGCGGCTGT CAAACCTCCA CTCTAACACT TCTCTCTCAG GGTCATCCAT TCCATGCAGG CTCACAGGGT GTAACAAGCT GGTGTTCTCT CCTTTATTGG CCTCTTCTAC CTTATCTGGC TCAACTGGTA CTAGCTTGTA GCACCATCCA AAGGTCAGTG GATATCTGAC CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT GGTAGATCCA CAGATCAAGG ATATCTTGTC TTCTTTGGGA GTGAATTAGC CCTTCCAGTC CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCTTCCTC CTCTTGTGCT TCTAGCCAGG CACAAGCAGC ATTGTTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT CTCGAGCAAT TTGGACTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC TGATTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA CTCCTCCGCG CAACTTTTTC GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT TTTCCGTCAT CGTCTTTCCG TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT CATCGTCGGG AAGACCTGCC ACGCCCGCGT CGAAGATGTT GGGGTGTTGT AACAATATCG ATTCCAATTC AGCGGGGGCC ACCTGATATC CTTTGTATTT AATTAAAGAC TTCAAGCGGT CAACTATGAA GAAGTGTTCG TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC CATCCTTGTC AATCAAGGCG TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA TAGGTCCTCT GACACATAAT TCGCCTCTCT GATTAACGCC CAGCGTTTTC CCGGTATCCA GATCCACAAC CTTCGCTTCA AAAAATGGAA CAACTTTACC GACCGCGCCC GGTTTATCAT CCCCCTCGGG TGTAATCAGA ATAGCTGATG TAGTCTCAGT GAGCCCATAT CCTTGTCGTA TCCCTGGAAG ATGGAAGCGT TTTGCAACCG CTTCCCCGAC TTCTTTCGAA AGAGGTGCGC CCCCAGAAGC AATTTCGTGT AAATTAGATA AATCGTATTT GTCAATCAGA GTGCTTTTGG CGAAGAATGA AAATAGGGTT GGTACTAGCA ACGCACTTTG AATTTTGTAA TCCTGAAGGG ATCGTAAAAA CAGCTCTTCT TCAAATCTAT ACATTAAGAC GACTCGAAAT CCACATATCA AATATCCGAG TGTAGTAAAC ATTCCAAAAC CGTGATGGAA TGGAACAACA CTTAAAATCG CAGTATCCGG AATGATTTGA TTGCCAAAAA TAGGATCTCT GGCATGCGAG AATCTGACGC AGGCAGTTCT ATGCGGAAGG GCCACACCCT TAGGTAACCC AGTAGATCCA GAGGAATTCA TTATCAGTGC AATTGTTTTG TCACGATCAA AGGACTCTGG TACAAAATCG TATTCATTAA AACCGGGAGG TAGATGAGAT GTGACGAACG TGTACATCGA CTGAAATCCC TGGTAATCCG TTTTAGAATC CATGATAATA ATTTTCTGGA TTATTGGTAA TTTTTTTTGC ACGTTCAAAA TTTTTTGCAA CCCCTTTTTG GAAACAAACA CTACGGTAGG CTGCGAAATG TTCATACTGT TGAGCAATTC ACGTTCATTA TAAATGTCGT TCGCGGGCGC AACTGCAACT CCGATAAATA ACGCGCCCAA CACCGGCATA AAGAATTGAA GAGAGTTTTC ACTGCATACG ACGATTCTGT GATTTGTATT CAGCCCATAT CGTTTCATAG CTTCTGCCAA CCGAACGGAC ATTTCGAAGT ATTCCGCGTA CGTGATGTTC ACCTCGATAT GTGCATCTGT AAAAGCAATT GTTCCAGGAA CCAGGGCGTA TCTCTTCATA GCCTTATGCA GTTGCTCTCC AGCGGTTCCA TCCTCTAGAG GATAGAATGG CGCCGGGCCT TTCTTTATGT TTTTGGCGTC TTCAGCGGCC GCGCCACCCA TCTTATAGCA AAATCCTTTC CAAGCCCTGT CTTATTCTTC TAGGTATGTG GCGAATAGCT CTACAAGCTC CTTGTACTAC TTCTATAACC CTATCTGTCC CCTCAGCTAC TGCTATGGCT GTGGCATTGA GCAAGCTAAC AGCACTATTC TTTAGCTCCT GACTCCAATA TTGTAGGAGA TTCCACCAAT ATTTGAGGGC TTCCCACCCC CTGCGTCCCA GAAGTTCCAC AATCCTCGTT ACAATCAAGA GTAAGTCTCT CAAGCGGTGG TAGCTGAAGA GGCACAGGCT CCGCAGATCG TCCCAGATAA GTGCTAAGGA TCCGTTCACT AATCGAATGG ATCTGTCTCT GTCTCTCTCT CCACCTTCTT CTTCTATTCC TTCGGGCCTG TCGGGTCCCC TCGGGATTGG GAGGTGGGTC TGAAACGATA ATGGTGAATA TCCCTGCCTA ACTCTATTCA CTATAGAAAG TACAGCAAAA ACTATTCTTA AACCTACCAA GCCTCCTACT ATCATTATGA ATAATTTTAT ATACCACAGC CAATTTGTTA TGTTAAACCA ATTCCACAAA CTTGCCCATT TATCTAATTC CAATAATTCT TGTTCATTCT TTTCTTGCTG GTTTTGCGAT TCTTCAATTA AGGAGTGTAT TAAGCTTGTG TAATTGTTAA TTTCTCTGTC CCACTCCATC CAGGTCATGT TATTCCAAAT CTGTTCCAGA GATTTATTAC TCCAACTAGC ATTCCAAGGC ACAGCAGTGG TGCAAATGAG TTTTCCAGAG CAACCCCAAA TCCCCAGGAG CTGTTGATCC TTTAGGTATC TTTCCACAGC CAGGATTCTT GCCTGGAGCT GTTTGATGCC CCAGACTGTG AGTTGCAACA GATGCTGTTG CGCCTCAATA GCCCTCAGCA AATTGTTCTG CTGCTGCACT ATATCAGACA ATAATTGTCT GGCCTGTACC GTCAGCGTCA TTGACGCTGC GCCCATAGTG CTTCCTGCTG CTCCCAAGAA CCCAAGGAAC AAAGCTCCTA TTCCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA TTGTCCCTCA TATCGCCTCC TCCAGGTCTG AAGATCTCGG ACCCATTGTT GTTATTACCA CCATCTCTTG TTAATAGCAG CCCAGTAATA TTTGATGAAC ATCTAATTTG TCCACTGATG GGAGGGGCAT ACATTGCTTT TCCTACTTCC TGCCACATGT TTATAAATTG TTTTATTCTG CATGGGAGTG TGATTGTGTC ACTTCCTTCA GTGTTATTTG ACCCTTCAGT ACTCCAAGTA CTATTAAACC AAGTACTATT AAACAGTTGT GTTGAATTAC AGTAGAAAAA TTCCCCTCCA CAATTAAAAC TGTGCGTTAC AATTTCTGGG TCCCCTCCTG AGGATTGCTT AAAGATTATT GTTTTATTAT TTCCAAATTG TTCTCTTAAT TTGCTAGCTA TCTGTTTTAA AGTGGCATTC CATTTTGCTC TACTAATGTT ACAATGTGCT TGTCTCATAT TTCCTATTTT TCCTATTGTA ACAAATGCTC TCCCTGGTCC CCTCTGGATA CGGATACTTT TTCTTGTATT GTTGTTGGGT CTTGTACAAT TAATTTCTAC AGATGTGTTC AGCTGTACTA TTATGGTTTT AGCATTGTCT GTGAAATTGG CAGATCTAAT TACTACATCT TCTTCTGCTA GACTGCCATT TAACAGCAGT TGAGTTGATA CTACTGGCCT GATTCCATGT GTACATTGTA CTGTGCTGAC ATTTGTACAT GGTCCTGTTC CATTGAACGT CTTATTATTA CATTTTAGAA TCGCAAAACC AGCCGGGGCA CAATAATGTA TGGGAATTGG CTCAAAGGAT ACCTTTGGAC AGGCCTGTGT AATGACTGAG GTGTTACAAC TTATCAACCT ATAGCTGGTA TTATCTATTG GTACTATATC AAGTTTATAA AAGAATGCAT ATTCTTTCTG CACCTTATCT CTTATGCTTG TGCTGATATT GAAAGAGCAG TTTTTTATCT CTCCTTTCTC CATTATCATT CTCCCGCTAC TACTATTGGT ATTAGTATCA TTCTTCAAAT CAGTGCACTT TAAACTAACA CAGAGTGGGG TTAATTTTAC ACATGGCTTT AGGCTTTGAT CCCATAAACT GATTATATCC TCATGCATCT GTTCTACCAT GTCATTTTTC CACATGTTAA AATTTTCTGT CACATTTACC AATACTACTT CTTGTGGGTT GGGGTCTGTG GGTACACAGG CATGTGTGGC CCAAACATTA TGTACCTCTG TATCATATGC TTTAGCATCT GATGCACAAA ATAGAGTGGT GGTTGCTTCC TTCCACACAG GTACCCCATA ATAGACTGTG ACCCACAATT TTTCTGTAGC ACTACAGATC ATCAATATCC CAAGGAGCAT GGTGCCCCAT TTCCACCCCC ATCTCCACAA GTGCTGATAC TTCTCCTTCA CTCTCATTGC CACTGTCTTC TGCTCTTTCT ATTAGTCTAT CAATTAACCT GTCTATTTTT CTTTGTCTTA ATATTTTCCT ATATTCTATG ATTACTATGG ACCACACAAC TATTGCTATT ATTATTGCTA CTACTAATGC TACTATTGCT ACTATTATAG GTTGCATTAC ATGTACTACT TACTGCTTTG ATAGAGAAGC TTGATGAGTC TGACTGTTCT GATGAGCTCT TCGTCGCTGT CTCCGCTTCT TCCTGCCATA GGAGATGCCT AAGGCTTTTG TCATGAAACA AACTTGGCAA TGAAAGCAAC ACTTTTTACA ATAGCAATTG GTACAAGCAG TTTTAGGCTG ACTTCCTGGA TGCTTCCAGG GCTCTAGTCT AGGATCTACT GGCTCCATTT CTTGCTCTCC TCTGTCGAGT AACGCCTATT CTGCTATGTC GACACCCAAT TCTGAAATGG ATAAACAGCA GTTGTTGCAG AATTCTTATT ATGGCTTCCA CTCCTGCCCA AGTATCCCCG TAAGTTTCAT AGATATGTTG TCCTAAGTTA TGGAGCCATA TCCTAGGAAA ATGTCTAACA GCTTCACTCT TAAGTTCCTC TAAAAGCTCT AGTGTCCATT CATTGTATGG CTCCCTCTGT GGCCCTTGGT CTTCTGGGGC TTGTTCCATC TGTCCTCTGT CAGTTTCCTA ACACTAGGCA AAGGTGGCTT TATCTGTTTT GGTTTTATTA ATGCTGCTAG TGCCAAGTAC TGTAGAGATC CTACCTTGTT ATGTCCTGCT TGATATTCAC ACCTAGGACT AACTATACGT CCTAATATGG TATTTCTTAT AGCAGATTCT GAAAAACAAT CAAAATAGTG CAGATGAATT AGTTGGTCTG CTAGGTCAGG GTCTACTTGT GTGCTATATC TCTTTTTCCT CCATTCTATG GAGACTCCCT GACCCAAATG CCAGTCTCTT TCTCCTGTAT GCAGACCCCA ATATGTTGTT ATTACTAATT TAGCATCCCC TAGTGGGATG TGTACTTCTG AACTTATTTT TGGATTAGTA CTTTCATAGT GATGTCTATA AAACCAGTCC TTAGCTTTCC TTGAAATATA CATATGGTGT TTTACTAATC TTTTCCATGT GTTAATCCTC ATCCTGTCTA CTTGCCACAC AATCATCACC TGCCATCTGT TTTCCATAAT CCCTGATGAT CTTTGCTTTT CTTCTTGGCA CTACTTTTAT GTCACTATTA TCTTGTATTA CTACTGCCCC TTCACCTTTC CAGAGGAGCT TTGCTGGTCC TTTCCAAACT GGATCTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TACTGCTGTC TTAAGATGTT CAGCCTGATC TCTTACCTGT CCTATAATTT TCTTTAATTC TTTATTCATA GATTCTATTA CTCCTTGACT TTGGGGATTG TAGGGAATGC CAAATTCCTG CTTGATCCCC GCCCACCAAC AGGCGGCCTT AACTGTAGTA CTGGTGAAAT TGCTGCCATT GTCTGTATGT ACTGTTTTTA CTGGCCATCT TCCTGCTAAT TTTAAGAGGA AGTATGCTGT TTCTTGCCCT GTCTCTGCTG GAATTACTTC TGCTTCTATA TATCCACTGG CTACATGAAC TGCTACCAAG ATAACTTTTC CTTCTAAATG TGTACAATCT AGCTGCCATA TTCCTGGGCT ACAGTCTACT TGTCCATGCA TGGCTTCCCC TTTTAGCTGA CATTTATCAC AGCTGGCTAC TATTTCTTTT GCTACTACAG GTGGTAGGTT AAAATCACTA GCCATTGCTC TCCAATTACT GTGATATTTC TCATGTTCTT CTTGGGCCTT ATCTATTCCA TCTAAAAATA GTACTTTCCT GATTCCAGCA CTGACCAATT TATCTACTTG TTCATTTCCT CCAATTCCTT TGTGTGCTGG TACCCATGCC AGGTAGACTT TTTCCTTTTT TATTAACTGC TCTATTATTT GACTGACTAA CTCTGATTCA CTCTTATCTG GTTGTGCTTG AATGATTCCC AATGCATATT GTGAGTCTGT CACTATGTTT ACTTCTAATC CCGAATCCTG CAAAGCTAGA TGAATTGCTT GTAACTCAGT CTTCTGATTT GTTGTGTCCG TTAGGGGGAC AACTTTTTGT CTTCCTCTGT CAGTTACATA TCCTGCTTTT CCTAATTTAG TTTCCCTATT GGCTGCCCCA TCTACATAGA AAGTTTCTGC TCCTATTATG GGTTCTTTCT CTAACTGGTA CCATAACTTC ACTAAGGGAG GGGTATTGAC AAACTCCCAC TCAGGAATCC AGGTGGCTTG CCAATACTCT GTCCACCATG CTTCCCATGT TTCCTTTTGT ATGGGTAATT TAAATTTAGG AGTCTTTCCC CATATTACTA TGCTTTCTGT GGCTATTTTT TGTACTGCCT CTGTTAATTG TTTCACATCA TTAGTGTGGG CACCCTTCAT TCTTGCATAC TTTCCTGTTT TCAGATTTTT AAATGGCTCT TGATAAATTT GATATGTCCA TTGGCCTTGC CCCTGCTTCT GTATTTCTGC TATTAAGTCT TTTGATGGGT CATAATACAC TCCATGTACC GGTTCTTTTA GAATCTCCCT GTTTTCTGCC AGTTCTAGCT CTGCTTCTTC TGTTAGTGGT ACTACTTCTG TTAGTGCTTT GGTTCCCCTA AGAAGTTTAC ATAATTGCCT TACTTTAATC CCTGCATAAA TCTGACTTGC CCAATTCAAT TTTCCCACTA ATTTCTGTAT GTCATTGACA GTCCAGCTGT CCTTTTCTGG CAGCACTATA GGCTGTACTG TCCATTTATC AGGATGGAGT TCATAACCCA TCCAAAGGAA TGGAGGTTCT TTCTGATGTT TTTTGTCTGG TGTGGTAAAT CCCCACCTCA ACAGATGTTG TCTCAGTTCC TCTATTTTTG TTCTATGCTG CCCTATTTCT AAGTCAGATC CTACATACAA ATCATCCATG TATTGATAGA TGACTATGTC TGGATTTTGT TTTCTAAAAG GCTCTAAGAT TTTTGTCATG CTACACTGGA ATATTGCTGG TGATCCTTTC CATCCCTGTG GAAGCACATT GTACTGATAT CTAATCCCTG GTGTCTCATT GTTTATACTA GGTATGGTAA ATGCAGTATA CTTCCTGAAG TCTTTATCTA AGGGAACTGA AAAATATGCA TCGCCCACAT CCAGTACTGT TACTGATTTT TTCTGTTTTA ACCCTGCAGG ATGTGGTATT CCTAATTGAA CTTCCCAGAA ATCTTGAGTT CTCTTATTAA GTTCTCTGAA ATCTACTAAT TTTCTCCATT TAGTACTGTC TTTTTTCTTT ATGGCAAATA CTGGAGTATT GTATGGATTT TCAGGCCCAA TTTTTGAAAT TTTTCCTTCC TTTTCCATTT CTGTACAAAT TTCTACTAAT GCTTTTATTT TTTCTTCTGT CAATGGCCAT TGTTTAACTT TTGGGCCATC CATTCCTGGC TTTAATTTTA CTGGTACAGT CTCAATAGGA CTAATGGGAA AATTTAAAGT GCAGCCAATC TGAGTCAACA GATTTCTTCC AATTATGTTG ACAGGTGTAG GTCCTACTAA TACTGTACCT ATAGCTTTAT GTCCGCAGAT TTCTATGAGT ATCTGATCAT ACTGTCTTAC TTTGATAAAA CCTCCAATTC CCCCTATCAT TTTTGGTTTC CATCTTCCTG GCAAATTCAT TTCTTCTAAT ACTGTATCAT CTGCTCCTGT ATCTAATAGA GCTTCCTTTA ATTGCCCCCC TATCTTTATT GTGACGAGGG GTCGCTGCCA AAGAGTGATC TGAGGGAAGC TAAAGGATAC AGTTCCTTGT CTATCGGCTC CTGCTTCTGA GAGGGAGTTG TTGTCTCTTC CCCAAACCTG AAGCTCTCTT CTGGTGGGGC TGTTGGCTCT GGTCTGCTCT GAAGAAAATT CCCTGGCCTT CCCTTGTGGG AAGGCCAGAT CTTCCCTAAA AAATTAGCCT GTCTCTCAGT ACAATCTTTC ATTTGGTGTC CTTCCTTTCC ACATTTCCAA CAGCCCTTTT TCCTAGGGGC CCTGCAATTT TTGGCTATGT GCCCTTCTTT GCCACAATTG AAACACTTAA CAGTCTTTCT TTGGTTCCTA AAATTGCCTT TCTGTATCAT TATGGTAGCT GGATTTGTTA CTTGGCTCAT TGCTTCAGCC AAAACTCTTG CTTTATGGCC GGGTCCCCCC ACTCCCTGAC ATGCTGTCAT CATTTCTTCT AGTGTCGCTC CTGGTCCCAA TGCTTTTAAA ATAGTCTTAC AATCTGGGTT CGCATTTTGG ACCAACAAGG TTTCTGTCAT CCAATTTTTT ACCTCTTGTG AAGCTTGCTC GGCTCTTAGA GTTTTATAGA ATCGGTCTAC ATAGTCTCTA AAGGGTTCCT TTGGTCCTTG TCTTATGTCC AGAATGCTGG TAGGGCTATA CATTCTTACT ATTTTATTTA ATCCCAGGAT TATCCATCTT TTATAGATTT CTCCTACTGG GATAGGTGGA TTATGTGTCA TCCATCCTAT TTGTTCCTGA AGGGTACTAG TAGTTCCTGC TATGTCACTT CCCCTTGGTT CTCTCATCTG GCCTGGTGCA ATAGGCCCTG CATGCACTGG ATGCAATCTA TCCCATTCTG CAGCTTCCTC ATTGATGGTC TCTTTTAACA TTTGCATGGC TGCTTGATGT CCCCCCACTG TGTTTAGCAT GGTATTTAAA TCTTGTGGGG TGGCTCCTTC TGATAATGCT GAAAACATGG GTATTACTTC TGGGCTGAAA GCCTTCTCTT CTACTACTTT TACCCATGCA TTTAAAGTTC TAGGTGATAT GGCCTGATGT ACCATTTGCC CCTGGAGGTT CTGCACTATA GGGTAATTTT GGCTGACCTG GCTGTTGTTT CCTGTGTCAG CTGCTGCTTG CTGTGCCTTT TTCTTACTTT TGTTTTGCTC TTCCTCTATC TTATCTAAGG CTTCCTTGGT GTCTTTTACA TCTATCCTTT GATGCACACA ATAGAGGACT GCTATTGTAT TATATAATGA TCTAAGTTCT TCTGATCCTG TCTGAAGGGA TGGTTGTAGC TGTCCCAGTA TTTGTCTACA GCCTTCTGAT GTCTCTAAAA GGCCAGGATT AACTGCGAAT CGTTCTAGCT CCCTGCTTGC CCATACTATA TGTTTTAGTT TATATTGTTT CTTTCCCCCT GGCCTTAACC GAATTTTTTC CCATTTATCT AATTCTCCCC CGCTTAATAC CGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC AGCAAGCCGA GTCCTGCGTC GAGAGATCTC CTCTGGCTTT ACTTTCGCTT TCAAGTCCCT GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTTGA GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GGCAAAAAGC AGCTGCTTAT ATGTAGCATC TGAGGGCTCG CCACTCCCCA GTCCCGCCCA GGCCACACCT CCCTGGAAAG TCCCCAGCGG AAAGTCCCTT GTAGAAAGCT CGATGTCAGC AGTCTTTGTA GTACTCCGGA TGCAGCTCTC GGGCCATGTG ACGAAATGCT AGGAGGCTGT CAAACTTCCA CACTAATACT TCTCCCTCCG GGTCCTCCAT CCCATGCTGG CTCATAGGGT GTAACAAGCT GTTGTTCTCT CCTTCATTGG CCTCTTCTAC TTGCTCTGGT TCAACTGGTA CTAACTTGAA GCACCATCCA AAGGTCAGTG GATATCTGAT CCCTGGCCCT GGTGTGTAGT TCTGCCAATC AGGGAAGTAG CCTTGTGTGT GGTAGATCCA CAGATCAAGG ATCTCTTGTC TTTTTTGGGA CCAAATTAGC CCTTCCAGCC TTTCCTGGTG TAAGTAGAAC TGGGGCTTTC CCTAGGTTCT GTTCATCAGG TGGATGGCTG GAAGCTGCGC TGGTCCCTCC CTCCACACCA AGGACTCTGA TGCGCAGGTG TGCTTGCACC TCCGACATGG CAGTTCCTCC GGCCATGTGC ACGTGGAGGG CGGCCCATTG TGGTAGCCTT GGGAGGTGTC TTGGTTATCT TGTGTCACTG TGACCTGTGG AGATCCAACT GAGGTAGTCA TCACCCCTCG GGGGACACAT TCCAAGACCC AGGTGGAGGC CTGAAATGCG ATAGTACTGA ACCCTGTATA TACTGTTTTC CCCTCTGGGA ACACACCTCT GATCAAATTT AACTTACAAG TTAGGCACAG TAGAGACTAA AAAAATTGTG AATCAAATGG AATGATTGTA AGGGTGCACT GTGATAAACC CCCTGTGAGG CTGACGACTG TCAGGGTGCA ATGTGATAAA CCCCCTGTGA GGCTGACGAC TGTCAGGGTG CACTGTGATA AACCCCCTGT AAAGCTGGTC TCTTTCTCTC GCAGTCTTAC CGCTCTGTGC GCACCTAGTT TCAGACCTTG GCTGAACGCA AGTAACTGAA ACTAGGGAAA GCAAAGCCGA GTGGGGGCAG CCGCTAGAAG GAAGGGGCAG CCTCAGGGCA CACTGTGGGG CTTGGCAGTT AGGGACCCTG CAGAGTCCAC ATGCCAGTTG GTTGGCAGCA CAGCAGAGCG CCCGGAAGGG GCACATGCCT TTGACAGGAG TTAGATGTGG TGGAGAGAGG CGCTTAAAAT TATTGAGGAC AAGATGGGTT ATTTGGTATT TAATGCTAAC AGCTGTATCT CCATTTTGAT GTGAAGGAGT GAGCTAGATT TTATTTATTT TTTATTATTT TATTTTTTAC CTTTAGGCTC
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