pHT43-SUMO-GFP
$142.00
In stock
SKU
PL67241
100μg liquid plasmid(Instantly):$163
| name | pHT43-SUMO-GFP | another name | apHT43-SUMO-GFP |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression,Secretion expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Chl | Fluorescent labeling | Green |
| Promoter | Pgrac | Replicon | pUC,repB |
| Competence | Stbl3 | Growth Temperature | 30°C |
| Vector backbone | 5′ sequencing primer | M13F：TGTAAAACGACGGCCAGT | |
| 3′ sequencing primer | pHT-R：agcaagtcatctccaaattctt | Copy number | high |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TTAAGTTATT GGTATGACTG GTTTTAAGCG CAAAAAAAGT TGCTTTTTCG TACCTATTAA TGTATCGTTT TAGAAAACCG ACTGTAAAAA GTACAGTCGG CATTATCTCA TATTATAAAA GCCAGTCATT AGGCCTATCT GACAATTCCT GAATAGAGTT CATAAACAAT CCTGCATGAT AACCATCACA AACAGAATGA TGTACCTGTA AAGATAGCGG TAAATATATT GAATTACCTT TATTAATGAA TTTTCCTGCT GTAATAATGG GTAGAAGGTA ATTACTATTA TTATTGATAT TTAAGTTAAA CCCAGTAAAT GAAGTCCATG GAATAATAGA AAGAGAAAAA GCATTTTCAG GTATAGGTGT TTTGGGAAAC AATTTCCCCG AACCATTATA TTTCTCTACA TCAGAAAGGT ATAAATCATA AAACTCTTTG AAGTCATTCT TTACAGGAGT CCAAATACCA GAGAATGTTT TAGATACACC ATCAAAAATT GTATAAAGTG GCTCTAACTT ATCCCAATAA CCTAACTCTC CGTCGCTATT GTAACCAGTT CTAAAAGCTG TATTTGAGTT TATCACCCTT GTCACTAAGA AAATAAATGC AGGGTAAAAT TTATATCCTT CTTGTTTTAT GTTTCGGTAT AAAACACTAA TATCAATTTC TGTGGTTATA CTAAAAGTCG TTTGTTGGTT CAAATAATGA TTAAATATCT CTTTTCTCTT CCAATTGTCT AAATCAATTT TATTAAAGTT CATTTGATAT GCCTCCTAAA TTTTTATCTA AAGTGAATTT AGGAGGCTTA CTTGTCTGCT TTCTTCATTA GAATCAATCC TTTTTTAAAA GTCAATATTA CTGTAACATA AATATATATT TTAAAAATAT CCCACTTTAT CCAATTTTCG TTTGTTGAAC TAATGGGTGC TTTAGTTGAA GAATAAAAGA CCACATTAAA AAATGTGGTC TTTTGTGTTT TTTTAAAGGA TTTGAGCGTA GCGAAAAATC CTTTTCTTTC TTATCTTGAT AATAAGGGTA ACTATTGCCG TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAATTC GAGCTCAGGC CTTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA AACCTGTCGT GCCAGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCC AGGGTGGTTT TTCTTTTCAC CAGTGAGACG GGCAACAGCT GATTGCCCTT CACCGCCTGG CCCTGAGAGA GTTGCAGCAA GCGGTCCACG CTGGTTTGCC CCAGCAGGCG AAAATCCTGT TTGATGGTGG TTGACGGCGG GATATAACAT GAGCTGTCTT CGGTATCGTC GTATCCCACT ACCGAGATAT CCGCACCAAC GCGCAGCCCG GACTCGGTAA TGGCGCGCAT TGCGCCCAGC GCCATCTGAT CGTTGGCAAC CAGCATCGCA GTGGGAACGA TGCCCTCATT CAGCATTTGC ATGGTTTGTT GAAAACCGGA CATGGCACTC CAGTCGCCTT CCCGTTCCGC TATCGGCTGA ATTTGATTGC GAGTGAGATA TTTATGCCAG CCAGCCAGAC GCAGACGCGC CGAGACAGAA CTTAATGGGC CCGCTAACAG CGCGATTTGC TGGTGACCCA ATGCGACCAG ATGCTCCACG CCCAGTCGCG TACCGTCTTC ATGGGAGAAA ATAATACTGT TGATGGGTGT CTGGTCAGAG ACATCAAGAA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGCACC GCCGCTTTAC AGGCTTCGAC GCCGCTTCGT TCTACCATCG ACACCACCAC GCTGGCACCC AGTTGATCGG CGCGAGATTT AATCGCCGCG ACAATTTGCG ACGGCGCGTG CAGGGCCAGA CTGGAGGTGG CAACGCCAAT CAGCAACGAC TGTTTGCCCG CCAGTTGTTG TGCCACGCGG TTGGGAATGT AATTCAGCTC CGCCATCGCC GCTTCCACTT TTTCCCGCGT TTTCGCAGAA ACGTGGCTGG CCTGGTTCAC CACGCGGGAA ACGGTCTGAT AAGAGACACC GGCATACTCT GCGACATCGT ATAACGTTAC TGGTTTCATC AAAATCGTCT CCCTCCGTTT GAATATTTGA TTGATCGTAA CCAGATGAAG CACTCTTTCC ACTATCCCTA CAGTGTTATG GCTTGAACAA TCACGAAACA ATAATTGGTA CGTACGATCT TTCAGCCGAC TCAAACATCA AATCTTACAA ATGTAGTCTT TGAAAGTATT ACATATGTAA GATTTAAATG CAACCGTTTT TTCGGAAGGA AATGATGACC TCGTTTCCAC CGGAATTAGC TTGGTACCAG CTATTGTAAC ATAATCGGTA CGGGGGTGAA AAAGCTAACG GAAAAGGGAG CGGAAAAGAA TGATGTAAGC GTGAAAAATT TTTTATCTTA TCACTTGAAA TTGGAAGGGA GATTCTTTAT TATAAGAATT GTGGAATTGT GAGCGGATAA CAATTCCCAA TTAAAGGAGG AAGGATCAAT GATTCAAAAA CGAAAGCGGA CAGTTTCGTT CAGACTTGTG CTTATGTGCA CGCTGTTATT TGTCAGTTTG CCGATTACAA AAACATCAGC CGTACTGGAT CCGATGCATC ATCATCATCA TCATGGCTCT CTTCAAGATT CTGAAGTTAA CCAAGAAGCT AAACCTGAAG TTAAACCTGA AGTTAAACCT GAAACACATA TCAACCTTAA AGTTTCTGAT GGCTCTTCTG AAATCTTCTT CAAAATCAAA AAAACAACAC CTCTTCGTCG TCTTATGGAA GCTTTCGCTA AACGTCAAGG CAAAGAAATG GATTCTCTTC GTTTCCTTTA CGATGGCATC CGTATCCAAG CTGATCAAGC TCCTGAAGAT CTTGATATGG AAGATAACGA TCATGAAGCT CATCGTGAAC AAATCGGCGG CATGTCTAAA GGCGAAGAAC TTTTCACAGG CGTTGTTCCT ATCCTTGTTG AACTTGATGG CGATGTTAAC GGCCATAAAT TCTCTGTTTC TGGCGAAGGC GAAGGCGATG CTACATACGG CAAACTTACA CTTAAATTCA TCTGCACAAC AGGCAAACTT CCTGTTCCTT GGCCTACACT TGTTACAACA TTCACATACG GCGTTCAATG CTTCTCTCGT TACCCTGATC ATATGAAACA ACATGATTTC TTCAAATCTG CTATGCCTGA AGGCTACGTT CAAGAACGTA CAATCTTCTT CAAAGATGAT GGCAACTACA AAACACGTGC TGAAGTTAAA TTCGAAGGCG ATACACTTGT TAACCGTATC GAACTTAAAG GCATCGATTT CAAAGAAGAT GGCAACATCC TTGGCCATAA ACTTGAATAC AACTACAACT CTCATAACGT TTACATCATG GCTGATAAAC AAAAAAACGG CATCAAAGTT AACTTCAAAA TCCGTCATAA CATCGAAGAT GGCTCTGTTC AACTTGCTGA TCATTACCAA CAAAACACAC CTATCGGCGA TGGCCCTGTT CTTCTTCCTG ATAACCATTA CCTTTCTACA CAATCTGCTC TTTCTAAAGA TCCTAACGAA AAACGTGATC ATATGGTTCT TCTTGAATTC GTTACAGCTG CTGGCATCAC ACATGGCATG GATGAACTTT ACAAATAATC TAGAGTCGAC GTCCCCGGGG CAGCCCGCCT AATGAGCGGG CTTTTTTCAC GTCACGCGTC CATGGAGATC TTTGTCTGCA ACTGAAAAGT TTATACCTTA CCTGGAACAA ATGGTTGAAA CATACGAGGC TAATATCGGC TTATTAGGAA TAGTCCCTGT ACTAATAAAA TCAGGTGGAT CAGTTGATCA GTATATTTTG GACGAAGCTC GGAAAGAATT TGGAGATGAC TTGCTTAATT CCACAATTAA ATTAAGGGAA AGAATAAAGC GATTTGATGT TCAAGGAATC ACGGAAGAAG ATACTCATGA TAAAGAAGCT CTAAAACTAT TCAATAACCT TACAATGGAA TTGATCGAAA GGGTGGAAGG TTAATGGTAC GAAAATTAGG GGATCTACCT AGAAAGCCAC AAGGCGATAG GTCAAGCTTA AAGAACCCTT ACATGGATCT TACAGATTCT GAAAGTAAAG AAACAACAGA GGTTAAACAA ACAGAACCAA AAAGAAAAAA AGCATTGTTG AAAACAATGA AAGTTGATGT TTCAATCCAT AATAAGATTA AATCGCTGCA CGAAATTCTG GCAGCATCCG AAGGGAATTC ATATTACTTA GAGGATACTA TTGAGAGAGC TATTGATAAG ATGGTTGAGA CATTACCTGA GAGCCAAAAA ACTTTTTATG AATATGAATT AAAAAAAAGA ACCAACAAAG GCTGAGACAG ACTCCAAACG AGTCTGTTTT TTTAAAAAAA ATATTAGGAG CATTGAATAT ATATTAGAGA ATTAAGAAAG ACATGGGAAT AAAAATATTT TAAATCCAGT AAAAATATGA TAAGATTATT TCAGAATATG AAGAACTCTG TTTGTTTTTG ATGAAAAAAC AAACAAAAAA AATCCACCTA ACGGAATCTC AATTTAACTA ACAGCGGCCA AACTGAGAAG TTAAATTTGA GAAGGGGAAA AGGCGGATTT ATACTTGTAT TTAACTATCT CCATTTTAAC ATTTTATTAA ACCCCATACA AGTGAAAATC CTCTTTTACA CTGTTCCTTT AGGTGATCGC GGAGGGACAT TATGAGTGAA GTAAACCTAA AAGGAAATAC AGATGAATTA GTGTATTATC GACAGCAAAC CACTGGAAAT AAAATCGCCA GGAAGAGAAT CAAAAAAGGG AAAGAAGAAG TTTATTATGT TGCTGAAACG GAAGAGAAGA TATGGACAGA AGAGCAAATA AAAAACTTTT CTTTAGACAA ATTTGGTACG CATATACCTT ACATAGAAGG TCATTATACA ATCTTAAATA ATTACTTCTT TGATTTTTGG GGCTATTTTT TAGGTGCTGA AGGAATTGCG CTCTATGCTC ACCTAACTCG TTATGCATAC GGCAGCAAAG ACTTTTGCTT TCCTAGTCTA CAAACAATCG CTAAAAAAAT GGACAAGACT CCTGTTACAG TTAGAGGCTA CTTGAAACTG CTTGAAAGGT ACGGTTTTAT TTGGAAGGTA AACGTCCGTA ATAAAACCAA GGATAACACA GAGGAATCCC CGATTTTTAA GATTAGACGT AAGGTTCCTT TGCTTTCAGA AGAACTTTTA AATGGAAACC CTAATATTGA AATTCCAGAT GACGAGGAAG CACATGTAAA GAAGGCTTTA AAAAAGGAAA AAGAGGGTCT TCCAAAGGTT TTGAAAAAAG AGCACGATGA ATTTGTTAAA AAAATGATGG ATGAGTCAGA AACAATTAAT ATTCCAGAGG CCTTACAATA TGACACAATG TATGAAGATA TACTCAGTAA AGGAGAAATT CGAAAAGAAA TCAAAAAACA AATACCTAAT CCTACAACAT CTTTTGAGAG TATATCAATG ACAACTGAAG AGGAAAAAGT CGACAGTACT TTAAAAAGCG AAATGCAAAA TCGTGTCTCT AAGCCTTCTT TTGATACCTG GTTTAAAAAC ACTAAGATCA AAATTGAAAA TAAAAATTGT TTATTACTTG TACCGAGTGA ATTTGCATTT GAATGGATTA AGAAAAGATA TTTAGAAACA ATTAAAACAG TCCTTGAAGA AGCTGGATAT GTTTTCGAAA AAATCGAACT AAGAAAAGTG CAATAAACTG CTGAAGTATT TCAGCAGTTT TTTTTATTTA GAAATAGTGA AAAAAATATA ATCAGGGAGG TATCAATATT TAATGAGTAC TGATTTAAAT TTATTTAGAC TGGAATTAAT AATTAACACG TAGACTAATT AAAATTTAAT GAGGGATAAA GAGGATACAA AAATATTAAT TTCAATCCCT ATTAAATTTT AACAAGGGGG GGATTAAAAT TTAATTAGAG GTTTATCCAC AAGAAAAGAC CCTAATAAAA TTTTTACTAG GGTTATAACA CTGATTAATT TCTTAATGGG GGAGGGATTA AAATTTAATG ACAAAGAAAA CAATCTTTTA AGAAAAGCTT TTAAAAGATA ATAATAAAAA GAGCTTTGCG ATTAAGCAAA ACTCTTTACT TTTTCATTGA CATTATCAAA TTCATCGATT TCAAATTGTT GTTGTATCAT AAAGTTAATT CTGTTTTGCA CAACCTTTTC AGGAATATAA AACACATCTG AGGCTTGTTT TATAAACTCA GGGTCGCTAA AGTCAATGTA ACGTAGCATA TGATATGGTA TAGCTTCCAC CCAAGTTAGC CTTTCTGCTT CTTCTGAATG TTTTTCATAT ACTTCCATGG GTATCTCTAA ATGATTTTCC TCATGTAGCA AGGTATGAGC AAAAAGTTTA TGGAATTGAT AGTTCCTCTC TTTTTCTTCA ACTTTTTTAT CTAAAACAAA CACTTTAACA TCTGAGTCAA TGTAAGCATA AGATGTTTTT CCAGTCATAA TTTCAATCCC AAATCTTTTA GACAGAAATT CTGGACGTAA ATCTTTTGGT GAAAGAATTT TTTTATGTAG CAATATATCC GATACAGCAC CTTCTAAAAG CGTTGGTGAA TAGGGCATTT TACCTATCTC CTCTCATTTT GTGGAATAAA AATAGTCATA TTCGTCCATC TACCTATCCT ATTATCGAAC AGTTGAACTT TTTAATCAAG GATCAGTCCT TTTTTTCATT ATTCTTAAAC TGTGCTCTTA ACTTTAACAA CTCGATTTGT TTTTCCAGAT CTCGAGGGTA ACTAGCCTCG CCGATCCCGC AAGAGGCCCG GCAGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CATGC
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