pCMV-ATP1A3(human)-3×FLAG-Neo
$99.00
In stock
SKU
PL67167
NM_152296.5;AHC2; ATP1A1; CAPOS; DEE99; DYT12; RDP
100μg liquid plasmid(Instantly):$120
| name | pCMV-ATP1A3(human)-3×FLAG-Neo | another name | apCMV-ATP1A3(human)-3×FLAG-Neo |
| Insert Size (bp) | 3042bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | CMV-F：CGCAAATGGGCGGTAGGCGTG | |
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATAGATGCCT CGAGTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTCTCTCT CCCCACTGAT ATATTTGATA ATTGTCCAGA ACCAGAGATG GCATCAGCCG GGGGGCTGAA GGGGAGTAAA AAAGAGCCCA GGGAGGTGGC TGGGGCGGGA GGAATGGATA GAGGGGTGAG GAGAGGGAGA GAAACTGACA CAGAAAAGAG AGAGGAGAGA GGTTTGGGGC AGGGGAGAGA AGCCAGCCAG AGTGGGGGCG GCAGGAGATA GTGGAGGGGG TGGGGGCCAA GGTGGGGCCA CAGGAAGAGA GGGCTCCTCC CCCCAGAATA CAAAATTGGG GGGACTGACA GGGGCGGTCC TGGGCCTGCG GGACGGGGAA GAGATGGGCG ATGTGGTGGG GCTGAGGTCA GTAGTAGGTT TCCTTCTCCA CCCAACCCCC TGGGTTCCTG CGCAGGATGA GTTTGCGGAT TTCGTCGTAG ACGAAGATGA GGAAACTGTA GGGGAAGGCA CAGAACCACC AGCTGGGCTT GAGAGGGTAC ATGCGCAGGG CCACGTCCAT GCCGGGGCAG TAGGACAGGA AGGCAGCCAG GGCCGTCTCC TCAAACAGCC CGAAGATCAG GATCTTGTTC TTCATGCCCT GCTGGAAGAC CGAGTTCCTC CGGGTCTTGC AGATGATCAG ATCGGCCCAC TGGACGACAA CGATGCTCAC AAAGAAGGCC GTGTGGCAGG TGAACTCCAC CACCTTCCTC TGCTCGTATG TCCACTGCTG CCCGTAACTG TCTTCCAGGT CATTGACGGT GCGGTCATCC CAGTTCAGCC GGATGCCCAC CAGGTTGCCG GGCAAGAAGC CATTTTCTGC CAGGATCACA AAGTAAGAGA AGAAGCCACC GAGAGCCTGG ATCATTCCAA TCTGCCCGTA GGCCATGCTG ATGAGTCTCT CATTGACCAA TTTGTCCGTC CGCGGGTTCC TGGGCTGTCT CTTCATGATG TCGCTTTCGG CAGCCTCGTA CGCCAGTGAG ATGGCAGGGA CCATGTCAGT GCCCAGATCG ATGCAGAGGA TGGTGATGGT GCCCAGGGGC AGCGGGATGT TGGCCATGAT GAACAGCAGG AAGGGCGTGA TCTCCGGGAT ATTGCTGGTC AGGGTGTAGG CAATGGACTT CTTTAGGTTG TCGAAGATCA GGCGGCCCTC CTCCACCCCT GTGACGATGG AGGCAAAGTT GTCGTCCAGC AGGATCATGT CAGCTGCCTG CTTGGAGACG TCAGAGCCAG CGATGCCCAT GGCCACCCCA ATGTCGGCCT TCTTCAGAGC GGGGGAGTCG TTCACACCAT CCCCGGTCAC AGCCACAATT GCACCCTGTC TCTGACAGCC CTCCACAATG ATGAGCTTCT GCTGGGGGGA TGTGCGGGCG AAGACGATCT CGGTGTGATT CTGCAGGATC TCGTCGATTT GCTCGGAGGT GAAGTCCTTG AGGTCGGTGC CGTGGATCAC GCAGGCCTTG GCATCCCGGG GGTTAACCTG GCTGACGGGA ATGTTGAGCC GGGCGGCGAT GTCCTCCACA GTCTCGTTGC CCTCAGAGAT GATGCCCACA CCCTTGGCAA TGGCCTTGGC CGTGATGGGG TGATCGCCGG TGACCATGAT GACCTTGATG CCTGCGCTGC GACACTTGCC CACCGCGTCA GGGACGGCTG CCCGGGGTGG GTCGATCATG GACATGAGGC CCACAAAGCA GAGGTTGTCC GTGGTGAAGT TCACGTCATC ACAGTCGAAG GCAAAGCCCT TGGGGAACTG CTCCTCGGGC AGGTAATAAT GGCAGAAACC AAGCACGCGC TCGCCCAGGC CACCGAGCTC AAGGTAGGCA TTCTGGAAGG CCTCCTTCAT TTCCTCGTCC AGAGGCTGCT CCTTGCCCTG TAGCAGGATG GTGGAGCAGC GGTCCAGGAT GCGCTCGGGG GCACCCTTCA TCACCAGCAG GTATCGGTTG TCGTTGGGGT CCTCGGTCTC ATGGATGGAG AGCTGGTATT TGTTGGTGGA ATTGAAGGGA ATCTCAGCCA CTTTCTTGTT GCGTTCACGC ATCAGCTTCA CGGAGCCAGA GGACAGCTCG ATGCACTTGA GCAGGGCAGA CTCAGACGCA TCCCCAGCCA CATCCCTCTT GAGCACAGGG ATGTTGTCCT GACCACCCTT GAAGACAGCG CGATTGCAGA GCCCAGCGAT GTGAGACAGG GCCACCCAGG TGTGCGAACT CTTGTCAAAT GAGGTCCCTG ACTGGTCCTC AGTGGTGTCA GCCTCGTGGA TCTGGTTGTC AAACCACATG TGGGCGACTG TCATGCGGTT CTGAGTGAGG GTCCCTGTCT TATCTGAGCA GATGGTGGAC GTGGAGCCCA GGGTTTCTAC AGCCTCCAGG TTCTTCACCA GGCAGTTCTT CCGGGCCATG CGCTTGGCGG TCAGCGTCAG ACACACAGTG ACAGTGGCCA GCAGACCCTC TGGGACATTG GCCACGATGA TGCCGATGAG GAAGATGACA GCCTCAAGCC AGGTGTATCC GAGAATGAGG GAGAGGATGA AGAAGGAGAC ACCCAGGAAG ACAGCCACGC CGGTGATGAG CTGGATGAAG TGCTCAATCT CGATGGCGAT GGGCGTCTTG CCCACCTCCA GCCCTGATGC CAGGGTGGCG ATACGGCCCA TGACAGTGCG GTCGCCCGTG GCCACCACCA CGCCCCGAGC CGTGCCTTCC ACACAGTTGG TGGAAAAGAA GGTGATGTTC CGAGTCTCCA AGGGGTTGTC GTGCGTGCAG TCGGGAGAGC GAGTCTGGGG CTCGGATTCG CCAGTCAGGG AGGAGTTGTC CACCTTGCAG CCGTGGGCTG AGATGATCCG CAGGTCAGCT GGCACTCGGT CTCCACCCTT GATCTCCACC AGGTCCCCGA CCACCACCTC CTCAGCGTTC ACCTGCATCT TCTCACCTTC CCGGATCACC AGGGCTTGCT GGGGCACCAT GTTCTTGAAG GACTCCATGA TCTTGGAGCT CTTGGCCTCC TGGTAGTAGG AGAAGCAGCC AGTGATGATC ACCACGGCCG CCAGCACGAT GCCCAGGTAC AGGTTGTCAC CAGAGGGGTC GTCCTCGGTG CCCGCCTGGA TACCGTAGGC CAGGAAGCAG AGGATAGCCC CGATCCACAG CAGGATGGAG AAGCCCCCGA AGAGCTGCCG GCAAAACTTG ACCCACTCTG GGGTGGTAGG CGGTGGCGTG AGTGCGTTAG GCCCATCCCG GGCCAGGATC TCCTGGGCTT TGCTGTGGGT CAAACCCTGC ACACAGTCTG TGTTGTATTT CCGGCAGACC TCTTCCACTG ACATCTTGTG CTCTGTCATA GCCACCTCCT TCTTGAGGTC ATCCAGGTCC CGGCGCTCCT TGCCCTTGTT CTTCTTGGGT GAGTCCTTGT CATCTTTCTT GTCCCCCATC TTGGCGGCTC CGCGGCGAGA GAGCCAGGCG GGCGGGGCGG GGACCTCGGG GCGGGCTCAG GCTCAGGCTT GGGCTGGGAG CCTCTGCAGC GCCCGCGCCT CGGTAGGTGC GCGCGCCTCG TGCCGAATTC GCCACCCAGG CCTCGCACGT GTTTT
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
