pWH1520
$113.00
In stock
SKU
PL67166
100μg liquid plasmid(Instantly):$134
| name | pWH1520 | another name | apWH1520 |
| Insert Size (bp) | Plasmid host | Bacillus subtilis | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AATGACAAAT GGTCCAAACT AGTACTAATA AAATTAATCA TTTTGAAAGC GCAAACAAAG TTTTATACGA AGGTAAAGAT TCTAAAAATC CTTTAGCTTT TAAATACTAT AACCCTGAAG AAGTAGTAGG CGGTAAAACG ATGAAAGATC AGCTGCGTTT TTCTGTTGCT TACTGGCACC AGTTTACAGC AGATGGTACG GATCAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG ACCTGCAGGC ATGCAAGCTT TCGCGAGCTC GAGATCTAGA TATCGATGAA TTGATCCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCACTGGA CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGGCAGA ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG GCGCATCTCG GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCAATGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTCC TGTTATAAAA AAAGGATCAA TTTTGAACTC TCTCCCAAAG TTGATCCCTT AACGATTTAG AAATCCCTTT GAGAATGTTT ATATACATTC AAGGTAACCA GCCAACTAAT GACAATGATT CCTGAAAAAA GTAATAACAA ATTACTATAC AGATAAGTTG ACTGATCAAC TTCCATAGGT AACAACCTTT GATCAAGTAA GGGTATGGAT AATAAACCAC CTACAATTGC AATACCTGTT CCCTCTGATA AAAAGCTGGT AAAGTTAAGC AAACTCATTC CAGCACCAGC TTCCTGCTGT TTCAAGCTAC TTGAAACAAT TGTTGATATA ACTGTTTTGG TGAACGAAAG CCCACCTAAA ACAAATACGA TTATAATTGT CATGAACCAT GATGTTGTTT CTAAAAGAAA GGAAGCAGTT AAAAAGCTAA CAGAAAGAAA TGTAACTCCG ATGTTTAACA CGTATAAAGG ACCTCTTCTA TCAACAAGTA TCCCACCAAT GTAGCCGAAA ATAATGACAC TCATTGTTCC AGGGAAAATA ATTACACTTC CGATTTCGGC AGTACTTAGC TGGTGAACAT CTTTCATCAT ATAAGGAACC ATAGAGACAA ACCCTGCTAC TGTTCCAAAT ATAATTCCCC CACAAAGAAC TCCAATCATA AAAGGTATAT TTTTCCCTAA TCCGGGATCA ACAAAAGGAT CTGTTACTTT CCTGATATGT TTTACAAATA TCAGGAATGA CAGCACGCTA ACGATAAGAA AAGAAATGCT ATATGATGTT GTAAACAACA TAAAAAATAC AATGCCTACA GACATTAGTA TAATTCCTTT GATATCAAAA TGACCTTTTA TCCTTACTTC TTTCTTTAAT AATTTCATAA GAAACGGAAC AGTGATAATT GTTATCATAG GAATGAGTAG AAGATAGGAC CAATGAATAT AATGGGCTAT CATTCCACCA ATCGCTGGAC CGACTCCTTC TCCCATGGCT ACTATCGATC CAATAAGACC AAATGCTTTA CCCCTATTTT CCTTTGGAAT ATAGCGCGCA ACTACAACCA TTACGAGTGC TGGAAATGCA GCTGCACCAG CCCCTTGAAT AAAACGAGCC ATAATAAGTA AGGAAAAGAA AGAATGGCCA ACAAACCCAA TTACCGACCC GAAACAATTT ATTATAATTC CAAATAGGAG TAACCTTTTG ATGCCTAATT GATCAGATAG CTTTCCATAT ACAGCTGTTC CAATGGAAAA GGTTAACATA AAGGCTGTGT TCACCCAGTT TGTACTCGCA GGTGGTTTAT TAAAATCATT TGCAATATCA GGTAATGAGA CGTTCAAAAC CATTTCATTT AATACGCTAA AAAAAGATAA AATGCAAAGC CAAATTAAAA TTTGGTTGTG TCGTAAATTC GATTGTGAAT AGGATGTATT CACATTTCAC CCTCCAATAA TGAGGGCAGA CGTAGTTTAT AGGGTTAATG ATACGCTTCC CTCTTTTAAT TGAACCCTGT TACATTCATT ACACTTCATA ATTAATTCCT CCTAAACTTG ATTAAAACAT TTTACCACAT ATAAACTAAG TTTTAAATTC AGTATTTCAT CACTTATACA ACAATATGGC CCGTTTGTTG AACTACTCTT TAATAAAATA ATTTTTCCGT TCCCAATTCC ACATTGCAAT AATAGAAAAT CCATCTTCAT CGGCTTTTTC GTCATCATCT GTATGAATCA AATCGCCTTC TTCTGTGTCA TCAAGGTTTA ATTTTTTATG TATTTCTTTT AACAAACCAC CATAGGAGAT TAACCTTTTA CGGTGTAAAC CTTCCTCCAA ATCAGACAAA CGTTTCAAAT TCTTTTCTTC ATCATCGGTC ATAAAATCCG TATCCTTTAC AGGATATTTT GCAGTTTCGT CAATTGCCGA TTGTATATCC GATTTATATT TATTTTTCGG TCGAATCATT TGAACTTTTA CATTTGGATC ATAGTCTAAT TTCATTGCCT TTTTCCAAAA TTGAATCCAT TGTTTTTGAT TCACGTAGTT TTCTGTATTC TTAAAATAAG TTGGTTCCAC ACATACCAAT ACATGCATGT GCTGATTATA AGAATTATCT TTATTATTTA TTGTCACTTC CGTTGCACGC ATAAAACCAA CAAGATTTTT ATTAATTTTT TTATATTGCA TCATTCGGCG AAATCCTTGA GCCATATCTG ACAAACTCTT ATTTAATTCT TCGCCATCAT AAACATTTTT AACTGTTAAT GTGAGAAACA ACCAACGAAC TGTTGGCTTT TGTTTAATAA CTTCAGCAAC AACCTTTTGT GACTGAATGC CATGTTTCAT TGCTCTCCTC CAGTTGCACA TTGGACAAAG CCTGGATTTA CAAAACCACA CTCGATACAA CTTTCTTTCG CCTGTTTCAC GATTTTGTTT ATACTCTAAT ATTTCAGCAC AATCTTTTAC TCTTTCAGCC TTTTTAAATT CAAGAATATG CAGAAGTTCA AAGTAATCAA CATTAGCGAT TTTCTTTTCT CTCCATGGTC TCACTTTTCC ACTTTTTGTC TTGTCCACTA AAACCCTTGA TTTTTCATCT GAATAAATGC TACTATTAGG ACACATAATA TTAAAAGAAA CCCCCATCTA TTTAGTTATT TGTTTGGTCA CTTATAACTT TAACAGATGG GGTTTTTCTG TGCAACCAAT TTTAAGGGTT TTCAATACTT TAAAACACAT ACATACCAAC ACTTCAACGC ACCTTTCAGC AACTAAAATA AAAATGACGT TATTTCTATA TGTATCAAGA TAAGAAAGAA CAAGTTCAAA ACCATCAAAA AAAGACACCT TTTCAGGTGC TTTTTTTATT TTATAAACTC ATTCCCTGAT CTCGACTTCG TTCTTTTTTT ACCTCTCGGT TATGAGTTAG TTCAAATTCG TTCTTTTTAG GTTCTAAATC GTGTTTTTCT TGGAATTGTG CTGTTTTATC CTTTACCTTG TCTACAAACC CCTTAAAAAC GTTTTTAAAG GCTTTTAAGC CGTCTGTACG TTCCTTAAGG AATTCTCATG TTTGACAGCT TATCATCGAT AAGCTTTAAT GCGGTAGTTT ATCACAGTTA AATTGCTAAC GCAGTCAGGC ACCGTGTATG AAATCTAACA ATGCGCTCAT CGTCATCCTC GGCACCGTCA CCCTGGATGC TGTAGGCATA GGCTTGGTTA TGCCGGTACT GCCGGGCCTC TTGCGGGATA TCGTCCATTC CGACAGCATC GCCAGTCACT ATGGCGTGCT GCTAGCGCTA TATGCGTTGA TGCAATTTCT ATGCGCACCC GTTCTCGGAG CACTGTCCGA CCGCTTTGGC CGCCGCCCAG TCCTGCTCGC TTCGCTACTT GGAGCCACTA TCGACTACGC GATCATGGCG ACCACACCCG TCCTGTGGAT CTGACGCGTG TAACTGCGAA GGTAAAACGG GTGATTGAAA ATAAACTAAC AAATGAAGAG GAACGAAAGA GAAGTCTAGA ATTTGTTACG TTTATATCGG ATGAATTACT GCAAAATGAT ACGGAGCCTC GCACATTTAT TATTCAGAAC TCTCTTTTAT CGCTAGAGAA AATCCATACT CTCGCACAGA CCAAAGAGGT AGAGGAATAT AAAGAAGTCA TAACTACTCT GACAAGACAT GATTCATAAT TTGAAAAGCA GGTAAACTAA CCCAAAAGGC AAGACTCTCC GTAGTTTAGA AAAGAGCAGG TTGCTAATAA CATATAAACA GCCAGTTGCC GTTATGATAG GTGACTGGCT GCATGGGATG AAAAGGTGAG GGTGGAGACA GACATAACAC TCTTAATAGA AGAGGGTAAT TCTTTCTCTT TTATAGAAAA TCAATTAATT GAAAGTAGCT CCTTCATTCT TAAGATCAAC GTGATATAGG TTTGCTAACC TTTGCGTTCA CTTAACTAAC TTATAGGGGT AACACTTAAA AAAGAATCAA TAACGATAGA AACCGCTCCT AAAGCAGGTG CATTTTTTCC TAACGAAGAA GGCAATAGTT CACATTTATT GTCTAAATGA GAATGGACTC TAGAAGAAAC TTCGTTTTTA ATCGTATTTA AAACAATGGG ATGAGATTCA ATTATATGAT TTCTCAAGAT AACAGCTTCT ATATCAAATG TATTAAGGAT ATTGGTTAAT CCAATTCCGA TATAAAAGCC AAAGTTTTGA AGTGCATTTA ACATTTCTAC ATCATTTTTA TTTGCGCGTT CCACAATCTC TTTTCGAGAA ATATTCTTTT CTTCTTTAGA GAGCGAAGCC AGTAACGCTT TTTCAGAAGC ATATAATTCC CAACAGCCTC GATTTCCACA GCTGCATTTG GGTCCATTAA AATCTATCGT CATATGACCC ATTTCCCCAG AAAAACCCTG AACACCTTTA TACAATTCGT TGTTAATAAC AAGTCCAGTT CCAATTCCGA TATTAATACT GATGTAAACG ATGTTTTCAT AGTTTTTTGT CATACCAAAT ACTTTTTCAC CGTATGCTCC TGCATTAGCT TCATTTTCAA CAAAAACCGG AACATTAAAC TCACTCTCAA TTAAAAACTG CAAATCTTTG ATATTCCAAT TTAAGTTAGG CATGAAAATA ATTTGCTGAT GACGATCTAC AAGGCCTGGA ACACAAATTC CTATTCCGAC TAGACCATAA GGGGACTCAG GCATATGGGT TACAAAACCA TGAATAAGTG CAAATAAAAT CTCTTTTACT TCACTAGCGG AAGAACTAGA CAAGTCAGAA GTCTTCTCGA GAATAATATT TCCTTCTAAG TCGGTTAGAA TTCCGTTAAG ATAGTCGACT CCTATATCAA TACCAATCGA GTAGCCTGCA TTCTTATTAA AAACAAGCAT TACAGGTCTT CTGCCGCCTC TAGATTGCCC TGCCCCAATT TCAAAAATAA AATCTTTTTC AAGCAGTGTA TTTACTTGAG AGGAGACAGT AGACTTGTTT AATCCTGTAA TCTCAGAGAG AGTTGCCCTG GAGACAGGGG AGTTCTTCAA AATTTCATCT AATATTAATT TTTGATTCAT TTTTTTTACT AAAGCTTGAT CTGCAATTTG AATAATAACC ACTCCTTTGT TTATCCACCG AACTAAGTTG GTGTTTTTTG AAGCTTGAAT TAGATATTTA AAAGTATCAT ATCTAATATT ATAACTAAAT TTTCTAAAAA AAACATTGAA ATAAACATTT ATTTTGTATA TGATGAGATA AAGTTAGTTT ATTGGATAAA CAAACTAACT CAATTAAGAT AGTTGATGGA TAAACTTGTT CACTTAAATC AAAGGGGGA
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