pMD2.G-Kan
$99.00
In stock
SKU
PL67073
100μg liquid plasmid(Instantly):$120
| name | pMD2.G-Kan | another name | apMD2.G-Kan |
| Insert Size (bp) | Plasmid host | Mammary cell,Lentiviral | |
| Plasmid uses | packaging aid | Fragment type | ORF |
| Fragment species | Virus | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GGATCCCCTG AGGGGGCCCC CATGGGCTAG AGGATCCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGA GCTTGGCCCA TTGCATACGT TGTATCCATA TCATAATATG TACATTTATA TTGGCTCATG TCCAACATTA CCGCCATGTT GACATTGATT ATTGACTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCC CTCGAAGCTT ACATGTGGTA CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG ACCCTTGATG TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA GAAGTAACAG GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA AATGCTTTCT TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC TCTTTCTTTC AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA TAACAGTGAT AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC ATATAAATTG TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA CCATTCTGCT TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC CCTTTTGCTA ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT GGTCTGTGTG CTGGCCCATC ACTTTGGCAA AGCACGTGAG ATCTGAATTC TGACACTATG AAGTGCCTTT TGTACTTAGC CTTTTTATTC ATTGGGGTGA ATTGCAAGTT CACCATAGTT TTTCCACACA ACCAAAAAGG AAACTGGAAA AATGTTCCTT CTAATTACCA TTATTGCCCG TCAAGCTCAG ATTTAAATTG GCATAATGAC TTAATAGGCA CAGCCATACA AGTCAAAATG CCCAAGAGTC ACAAGGCTAT TCAAGCAGAC GGTTGGATGT GTCATGCTTC CAAATGGGTC ACTACTTGTG ATTTCCGCTG GTATGGACCG AAGTATATAA CACAGTCCAT CCGATCCTTC ACTCCATCTG TAGAACAATG CAAGGAAAGC ATTGAACAAA CGAAACAAGG AACTTGGCTG AATCCAGGCT TCCCTCCTCA AAGTTGTGGA TATGCAACTG TGACGGATGC CGAAGCAGTG ATTGTCCAGG TGACTCCTCA CCATGTGCTG GTTGATGAAT ACACAGGAGA ATGGGTTGAT TCACAGTTCA TCAACGGAAA ATGCAGCAAT TACATATGCC CCACTGTCCA TAACTCTACA ACCTGGCATT CTGACTATAA GGTCAAAGGG CTATGTGATT CTAACCTCAT TTCCATGGAC ATCACCTTCT TCTCAGAGGA CGGAGAGCTA TCATCCCTGG GAAAGGAGGG CACAGGGTTC AGAAGTAACT ACTTTGCTTA TGAAACTGGA GGCAAGGCCT GCAAAATGCA ATACTGCAAG CATTGGGGAG TCAGACTCCC ATCAGGTGTC TGGTTCGAGA TGGCTGATAA GGATCTCTTT GCTGCAGCCA GATTCCCTGA ATGCCCAGAA GGGTCAAGTA TCTCTGCTCC ATCTCAGACC TCAGTGGATG TAAGTCTAAT TCAGGACGTT GAGAGGATCT TGGATTATTC CCTCTGCCAA GAAACCTGGA GCAAAATCAG AGCGGGTCTT CCAATCTCTC CAGTGGATCT CAGCTATCTT GCTCCTAAAA ACCCAGGAAC CGGTCCTGCT TTCACCATAA TCAATGGTAC CCTAAAATAC TTTGAGACCA GATACATCAG AGTCGATATT GCTGCTCCAA TCCTCTCAAG AATGGTCGGA ATGATCAGTG GAACTACCAC AGAAAGGGAA CTGTGGGATG ACTGGGCACC ATATGAAGAC GTGGAAATTG GACCCAATGG AGTTCTGAGG ACCAGTTCAG GATATAAGTT TCCTTTATAC ATGATTGGAC ATGGTATGTT GGACTCCGAT CTTCATCTTA GCTCAAAGGC TCAGGTGTTC GAACATCCTC ACATTCAAGA CGCTGCTTCG CAACTTCCTG ATGATGAGAG TTTATTTTTT GGTGATACTG GGCTATCCAA AAATCCAATC GAGCTTGTAG AAGGTTGGTT CAGTAGTTGG AAAAGCTCTA TTGCCTCTTT TTTCTTTATC ATAGGGTTAA TCATTGGACT ATTCTTGGTT CTCCGAGTTG GTATCCATCT TTGCATTAAA TTAAAGCACA CCAAGAAAAG ACAGATTTAT ACAGACATAG AGATGAACCG ACTTGGAAAG TAACTCAAAT CCTGCACAAC AGATTCTTCA TGTTTGGACC AAATCAACTT GTGATACCAT GCTCAAAGAG GCCTCAATTA TATTTGAGTT TTTAATTTTT ATGGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT GCCCTGGCCC ACAAGTTTCA CTAAGCTCGC TTCCTTGCTG TCCAATTTCT ATTAAAGGTT CCTTGGTTCC CTAAGTCCAA CTACTAAACT GGGGGATATT ATGAAGGGCC TTGAGCATCT GGATTCTGCC TAATAAAAAA CATTTATTTT CATTGCAATG ATGTATTTAA ATTATTTCTG AATATTTTAC TAAAAAGGGA ATGTGGGAGG TCAGTGCATT TAAAACATAA AGAAATGAAG AGCTAGTTCA AACCTTGGGA AAATACACTA TATCTTAAAC TCCATGAAAG AAGGTGAGGC TGCAAACAGC TAATGCACAT TGGCAACAGC CCTGATGCCT ATGCCTTATT CATCCCTCAG AAAAGGATTC AAGTAGAGGC TTGATTTGGA GGTTAAAGTT TGGCTATGCT GTATTTTACA TTACTTATTG TTTTAGCTGT CCTCATGAAT GTCTTTTCAC TACCCATTTG CTTATCCTGC ATCTCTCAGC CTTGACTCCA CTCAGTTCTC TTGCTTAGAG ATACCACCTT TCCCCTGAAG TGTTCCTTCC ATGTTTTACG GCGAGATGGT TTCTCCTCGC CTGGCCACTC AGCCTTAGTT GTCTCTGTTG TCTTATAGAG GTCTACTTGA AGAAGGAAAA ACAGGGGGCA TGGTTTGACT GTCCTGTGAG CCCTTCTTCC CTGCCTCCCC CACTCACAGT GACCCGGAAT CCCTCGACAT GGCAGTCTAG CACTAGTGCG GCCGCAGATC TGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGGTCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGT
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