pCMV-USP45(human)-FLAG
$84.00
In stock
SKU
PL66712
AJ583819.2
100μg liquid plasmid(Instantly):$105
| name | pCMV-USP45(human)-FLAG | another name | apCMV-USP45(human)-FLAG |
| Insert Size (bp) | 2442bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATGCATTAGT TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGATTACGC CAAGCTCGAA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGGCCAC CATGCGGGTG AAAGATCCAA CTAAAGCTTT ACCTGAGAAA GCCAAAAGAA GTAAAAGGCC TACTGTACCT CATGATGAAG ACTCTTCAGA TGATATTGCT GTAGGTTTAA CTTGCCAACA TGTAAGTCAT GCTATCAGCG TGAATCATGT AAAGAGAGCA ATAGCTGAGA ATCTGTGGTC AGTTTGCTCA GAATGTTTAA AAGAAAGAAG ATTCTATGAT GGGCAGCTAG TACTTACTTC TGATATTTGG TTGTGCCTCA AGTGTGGCTT CCAGGGATGT GGTAAAAACT CAGAAAGCCA ACATTCATTG AAGCACTTTA AGAGTTCCAG AACAGAGCCC CATTGTATTA TAATTAATCT GAGCACATGG ATTATATGGT GTTATGAATG TGATGAAAAA TTATCAACGC ATTGTAATAA GAAGGTTTTG GCTCAGATAG TTGATTTTCT CCAGAAACAT GCTTCTAAAA CACAAACAAG TGCATTTTCT AGAATCATGA AACTTTGTGA AGAAAAATGT GAAACAGATG AAATACAGAA GGGAGGAAAA TGCAGAAATT TATCTGTAAG AGGAATTACA AATTTAGGAA ATACTTGCTT TTTTAATGCA GTCATGCAGA ACTTGGCACA GACTTATACT CTTACTGATC TGATGAATGA GATCAAAGAA AGTAGTACAA AACTCAAGAT TTTTCCTTCC TCAGACTCTC AGCTGGACCC ATTGGTGGTG GAACTTTCAA GGCCTGGACC ACTGACCTCA GCCTTGTTCC TGTTTCTTCA CAGCATGAAG GAGACTGAAA AAGGACCACT TTCTCCTAAA GTTCTTTTTA ATCAGCTTTG TCAGAAGGCA CCTCGATTTA AAGATTTCCA GCAACAGGAC AGTCAGGAGC TTCTTCATTA TCTTCTGGAT GCAGTGAGGA CAGAAGAAAC AAAGCGAATA CAAGCTAGCA TTCTAAAAGC ATTTAACAAC CCAACTACTA AAACTGCTGA TGATGAAACT AGAAAAAAAG TCAAAGCATA TGGAAAAGAA GGTGTGAAAA TGAACTTCAT AGATCGGATC TTTATTGGTG AATTAACTAG CACGGTCATG TGTGAAGAAT GTGCAAATAT CTCCACGGTG AAAGATCCAT TCATTGATAT TTCACTTCCT ATAATAGAAG AAAGGGTTTC AAAACCTTTA CTTTGGGGAA GAATGAATAA ATATAGAAGT TTACGGGAGA CAGATCATGA TCGATACAGT GGCAATGTTA CTATAGAAAA TATTCATCAA CCTAGAGCTG CCAAGAAGCA TTCTTCATCT AAAGATAAGA GTCAACTAAT TCATGACCGA AAATGTATTA GAAAATTGTC ATCTGGAGAA ACTGTCACAT ACCAGAAAAA TGAAAACCTT GAAATGAATG GGGATTCTTT AATGTTTGCC AGCCTCATGA ATTCTGAGTC ACGTCTGAAT GAAAGCCCTA CTGATGACAG TGAAAAAGAA GCCAGCCATT CTGAAAGCAA TGTTGATGCT GACAGTGAGC CTTCAGAATC TGAAAGTGCT TCAAAGCAGA CTGGGCTGTT CAGATCCAGT AGTGGATCCG GTGTGCAGCC AGATGGACCC CTTTACCCTC TGTCAGCAGG TAAACTGCTG TACACCAAGG AGACTGACAG TGGTGATAAG GAAATGGCAG AAGCTATTTC TGAACTTCGT TTGAGCAGCA CTGTAACTGG AGATCAAGAT TTTGACAGAG AAAATCAGCC ACTAAATATT TCAAATAATT TATGTTTTTT AGAGGGGAAG CATTTGAGGT CTTATAGTCC CCAAAATGCT TTTCAGACCC TTTCTCAGAG CTATATAACT ACTTCTAAAG AATGTTCAAT TCAGTCCTGT CTCTACCAGT TTACATCTAT GGAATTACTA ATGGGGAATA ATAAGCTTCT ATGTGAGAAT TGTACTAAAA ACAAACAGAA GTACCAAGAA GAAACCAGTT TTGCAGAAAA GAAAGTAGAA GGAGTTTATA CTAATGCCAG GAAGCAATTG CTCATTTCTG CTGTTCCAGC TGTCCTAATT CTCCACCTGA AAAGATTTCA TAAGGCTGGC TTGAGTCTTC GTAAAGTAAA CAGACATGTA GATTTTCCAC TTATGCTCGA TTTAGCACCA TTCTGCTCTG CTACTTGTAA GAATGCAAGT GTGGGAGATA AAGTTCTCTA CGGTCTCTAT GGCATAGTGG AACATAGTGG CTCGATGAGA GAAGGCCACT ACACTGCTTA TGTGAAAGTG AGAACACCCT CCAGGAAATT ATCGGAACAT AACACTAAAA AGAAAAATGT GCCTGGTTTG AAAGCGGCTG ATAATGAATC AGCAGGCCAG TGGGTCCATG TTAGTGACAC TTACTTACAG GTGGTTCCAG AATCAAGAGC ACTTAGTGCA CAAGCCTACC TTCTTTTCTA TGAAAGAGTA TTACTCGAGT CTAGAGATTA CAAGGATGAC GACGATAAGT AAGGGCCCGG TACCTTAATT AATTAAGGTA CCAGGTAAGT GTACCCAATT CGCCCTATAG TGAGTCGTAT TACAATTCAC TCGATCGCCC TTCCCAACAG TTGCGCAGCC TGAATGGCGA ATGGAGATCC AATTTTTAAG TGTATAATGT GTTAAACTAC TGATTCTAAT TGTTTGTGTA TTTTAGATTC ACAGTCCCAA GGCTCATTTC AGGCCCCTCA GTCCTCACAG TCTGTTCATG ATCATAATCA GCCATACCAC ATTTGTAGAG GTTTTACTTG CTTTAAAAAA CCTCCCACAC CTCCCCCTGA ACCTGAAACA TAAAATGAAT GCAATTGTTG TTGTTAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTAACG CGTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG CCCACTACGT GAACCCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGCGCTAG GGCGCTGGCA AGTGTAGCGG TCACGCTGCG CGTAACCACC ACACCCGCCG CGCTTAATGC GCCGCTACAG GGCGCGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAATCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAACATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAACTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG GACTCTGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCTA GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGTG TTGGGTCGTT TGTTCATAAA CGCGGGGTTC GGTCCCAGGG CTGGCACTCT GTCGATACCC CACCGAGACC CCATTGGGGC CAATACGCCC GCGTTTCTTC CTTTTCCCCA CCCCACCCCC CAAGTTCGGG TGAAGGCCCA GGGCTCGCAG CCAACGTCGG GGCGGCAGGC CCTGCCATAG CCTCAGGTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC
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