pCMV-USP52(mouse)-FLAG
$99.00
In stock
SKU
PL66610
BC079841.1
100μg liquid plasmid(Instantly):$120
| name | pCMV-USP52(mouse)-FLAG | another name | apCMV-USP52(mouse)-FLAG |
| Insert Size (bp) | 3573bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Mouse | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGCTCCACC GCGGTGGCGG CCGCTCTAGC CCGGGCGGAT CCAAGCTTCT GCAGGAATTC GCCACCATGA ACTTTGAGGG TCTGGATCCT GGACTGGCCG AATTTTCCCC AGCTATGCAT TCTACCCTGG ACCCAGTCCT GGATGCCCAC CTGAATCCAA GTTTGCTACA GAATGTGGAG CTAGACCCAG AGGGAGTGGC CTTGGAGGCT CTTCCTGTCC AGGAGTCAGT GCACATAATG GAAGGTGTCT ACTCTGAGTT GCACAGCGTG GTGGCTGAAG TGGGAGTGCC TGTGTCTGTC TCCCACTTTG ATTTACATGA GGAGATGCTG TGGGTGGGGA GCCATGGGGG CCATGCCACC TCCTTCTTCG GCCCAGCTCT GGAGCGCTAC TCATCCTTTC AGGTCAATGG TGGTGACGAC ATTCGGCAAA TCCAGAGCCT GGAGAACGGT ATCCTCTTCC TCACCAAGAA CAACCTCAAG TACATGGCCC GTGGAGGGCT CATTATATTT GACTATTTGC TGGATGAAAA CGAGGATATG CACAGCGTCC TCCTGACAGA CAACAGCACT CTGCTCGTTG GGGGGCTGCA GAACCACGTA CTGGAGATTG ACCTGAACAC TGTCCAGGAG ACTCAGAAGT ATGCAGTCGA GACACCCGGA GTCACCATCA TGAGACAGAC AAATCGTTTC TTCTTCTGTG GCCACACATC TGGCAAGGTT TCCCTGCGAG ACCTCCGTAG TTTTAAAGTG GAGCATGAAT TTGATGCCTT CTCAGGGAGT CTGTCAGATT TTGATGTTCA TGGCAACCTG CTGGCTGCCT GCGGCTTCTC CAGTCGCCTT ACCGGCCTGG CCTGTGACCG TTTCCTCAAA GTGTACGATC TGCGCATGAT GCGTGCCATC ACACCTCTTC AAGTGCACGT GGATCCGGCC TTCTTACGAT TCATCCCCAC ATACACTTCC CGCCTTGCTA TCATCTCCCA GTCAGGTCAA TGCCAGTTTT GTGAACCCAC AGGCCTGGCC AACCCAGCCG ACATTTTCCA TGTGAATCCC GTGGGACCTT TGCTAATGAC GTTTGATGTG TCAGCTAGCA AGCAGGCCCT GGCCTTTGGG GATTCTGAGG GCTGCGTGCA TCTCTGGACT GATTCCCCTG AGCCGTCCTT CAACCCCTAC TCCCGAGAGA CTGAGTTTGC CCTGCCCTGT CTTGTGGACT CCTTGCCGCC TCTGGACTGG AGCCAGGATC TGCTGCCGCT TTCCCTCATT CCCGTCCCAC TTACCACTGA TGCACTGCTC TCTGACTGGC CTGCTGCCAA CTCTGCTCCC GCTCCCAGGC GAGCACCACC TGTGGATGCA GAAATTCTTC GAACCATGAA GAAAGTGGGC TTCATTGGCT ATGCTCCCAA CCCTCGCACC AGGCTGCGCA ACCAGATTCC CTATCGACTA AAGGAGTCAG ACCATGAATT TGACAACTTC AGCCAAGTCA CGGAGTCACC GACAGGGCGA GAAGAGGAGC CTCTCCACAC AGTTTCTAAG AAATACCGGA AGGTAACCAT CAAATATTCC AAGCTAGGCC TGGAAGACTT TGACTTCAAA CACTACAATA AGACTCTGTT TGCTGGGTTA GAGCCTCACA TCCCCAATGC CTACTGTAAC TGCATGATCC AGGTGCTCTA TTTCTTGGAG CCTGTTCGCT GTCTGATCCA GAACCACCTT TGCCAAAAGG AGTTCTGCCT AGCGTGCGAG CTCGGCTTCC TCTTCCACAT GCTAGACCTC TCTCGTGGCG ATCCTTGTCA GGGCAGTAAT TTCCTCCGAG CTTTCCGGAC CATCCCTGAG GCCTCAGCTC TGGGTCTCAT CCTTGCTGAC TCAGATGAGG CTTCAGGCAA GGGCAGTCTG GCCCGGCTCA TCCAGAGGTG GAACCGCTTC ATCCTCACTC AGCTACATCA GGATATGCAG GAGCTGGAAG TGCCCCAGGC TTATCGAGGT GCTGGAGGCA GTTTCTGCTC ATCAGGAGAC TCCATCATTG GGCAGCTGTT CAGTTGTGAG ATGGAGAACT GCAGTCTTTG CCGCTGCGGC AGCGAGACTG TGCGGGCCTC GTCCACCCTA CTCTTCACAC TCTCCTACCC TGAGGATAAA ACCGGGAAGA ACTATGACTT TGCTCAGGTG CTGAAGCGAA GTATCTGCCT GGAGCAGAAC ACACAGGCCT GGTGTGACAA CTGTGAGAAG TACCAGCCCA CAATTCAGAC CAGAAACATC CGACATCTGC CAGATATTCT TGTCATTAAC TGTGAAGTGA ATAGCTCAAA AGAGGCTGAT TTCTGGAGAC TGCAAGCTGA GGTTGCCTTC AAAATAGCGG TGAAAAAGTA CGGCGGGGAA ATGAAGAGCA AAGAGTTTGC TTTAGCTGAT CGGAAGGAAC TGAGGAGTCC AGAGGGCTTT CTGTGTTCCT CCATCGAGGA GCTGAAGAAT GTCTGGCTTC CATTTTCCAT CCGCATGAAG ATGACCAAGA ACAAAGGACT GGATGTTTGC AACTGGGCTG ACGAGCATGA GTGGGGTCCG GCCAGGGCAG AGGAGGAGCT TGGTGTCTAT GTATATGACC TGATGGCTAC TGTGGTACAC ATCCTGGACT CACGAACAGG CGGCAGCTTG GTGGCTCACA TCAAAGTCGG TGAGACCTAC CACCAGCGCA AGGAGGGCGT GACTCATCAG CAGTGGTATC TCTTCAATGA CTTCCTTATT GAACCTATTG ATAAGTATGA AGCCGTGCAG TTTGACATGA ATTGGAAAGT ACCTGCTATC CTGTATTATG TCAAAAGGAA TCTCAATTCC AGATACAATC TGAATATCAA GAACCCCATT GAGGCCAGTG TGCTGCTGGC AGAAGCCTCG CTGGCACGGA AGCAACGGAA AACACACACG ACCTTTATTC CATTGATGCT GAATGAGATG CCACAGGTCG GGGACCTGGT GGGCCTGGAT GCCGAGTTTG TCACTCTTAA TGAGGAAGAA GCAGAGTTAC GCAGTGATGG TACCAAGTCC ACCATTAAGC CAAGCCAGAT GTCAGTAGCA AGGATCACCT GTGTTCGGGG CCAAGGGCCT AACGAGGGCA TCCCCTTCAT TGATGACTAC ATCTCCACGC AGGAGCAGGT AGTAGATTAC TTGACTCAGT ACTCGGGGAT AAAGCCAGGA GACCTTGATG CCAAAATCTC CTCAAAGCAC CTCACAACCC TCAAGTCTAC CTACTTAAAG CTTCGCTTTC TCATTGATAT TGGAGTCAAG TTTGTGGGTC ATGGTCTGCA GAAGGACTTC CGGGTCATCA ACCTCATGGT GCCCAAGGAC CAAGTTCTTG ACACAGTCTA CCTGTTCCAC ATGCCCCGAA AACGAATGAT TTCCCTACGA TTCCTGGCCT GGTATTTTCT AGACCTGAAG ATTCAAGGTG AGACCCATGA CAGCATTGAG GATGCCCGCA CAGCCCTTCA ACTCTACCGG AAGTATCTGG AGCTGAGCAA GAACGGCACC GAGCCCGAGT CCTTCCACAA GGTGCTCAAG GGTCTCTATG AGAAGGGCCG AAAGATGGAC TGGAAGGTGC CTGAGCCTGA AAGCCAGACA AGTCCCAAGA ATGCAGCTGT CTTCTCAGTG CTGGCACTGC TCGAGTCTAG AGATTACAAG GATGACGACG ATAAGTAAGG GCCCGGTACC TTAATTAATT AAGGTACCAG G
Reviews
Write Your Own Review
Related Products
Check items to add to the cart or
