NM_016834.5;DDPAC; FTDP-17; MAPTL; MSTD
100μg liquid plasmid(Instantly):$192
| name | pLVX-TetOne-Puro-TRE3GS-MAPT(human)-3-P243S-Linker-mCherry | another name | apLVX-TetOne-Puro-TRE3GS-MAPT(human)-3-P243S-Linker-mCherry |
| Insert Size (bp) | 1149bp | Plasmid host | Mammary cell,Lentiviral |
| Plasmid uses | Protein expression,Tetracycline induction | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Puro | Fluorescent labeling | Red |
| Promoter | TRE3GS | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ACTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCAGTTTAT CGACTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAGGCAATAG CATCACAAAT TTCACAAATA AGGCATTTTT TTCACTGCAT TCTAGTTTTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGGAT CTCAAATCCC TCGGAAGCTG CGCCTGTCTT AGGTTGGAGT GATACATTTT TATCACTTTT ACCCGTCTTT GGATTAGGCA GTAGCTCTGA CGGCCCTCCT GTCTTAGGTT AGTGAAAAAT GTCACTCTCT TACCCGTCAT TGGCTGTCCA GCTTAGCTCG CAGGGGAGGT GGTCTGGATC CTCATCTGTG CCCCAGTTTG CTAGGGAGGT CGCAGTACTT GGCCACAGCC ATCTCGTGCT GCTCGACGTA GGTCTCTTTG TCGGCCTCCT TGATTCTTTC CAGTCTGTGG TCCACGAAGT GGAAGCCGGG CATCTTGAGG TTCTTAGCGG GTTTCTTGGA TCTGTATGTG GTCTTGAAGG AGCAGTGCAG GTAGCCCCCG CCCACGAGCT TCAGGGCCAT CTGGCTGTGG CCTCTCAGGC CGCCGTCAGC GGGGTACAGC ATCTCGGTGT TGGCCTCCCA GCCGCGTGTT TTCTTCTGCA TCACAGGGCC GTTGGATGGG AAGTTCACCC CGTTGATCTT GACGTTGTAG ATGATGCAGC CGTTCTGGAA GCTGGTGTCC TGGGTAGCGG TCAGCACGCC CCCGTCTTCG TATGTGGTGA TTCTCTCCCA TGTGAAGCCC TCAGGGAAGG ACTGCTTAAA GAAGTCGGGG ATGCCCTGGG TGTGGTTGAT GAAGGCTTTG CTGCCGTACA TGAAGCTGGT AGCCAGGATG TCGAAGGCGA AGGGGAGAGG GCCGCCCTCG ACCACCTTGA TCTTCATGGT CTGGGTGCCC TCGTAGGGCT TGCCTTCGCC CTCGGATGTG CACTTGAAGT GGTGGTTGTT CACGGTGCCC TCCATGTACA GCTTCATGTG CATGTTCTCC TTGATCAGCT CGCTCATACT CCCTCCGCCG CCCAAACCCT GCTTGGCCAG GGAGGCAGAC ACCTCGTCAG CTAGCGTGGC GAGCTGGGGC GAGTCTACCA TGTCGATGCT GCCGGTGGAG GAGACATTGC TGAGATGCCG TGGAGACGTG TCCCCAGACA CCACTGGCGA CTTGTACACG ATCTCCGCCC CGTGGTCTGT CTTGGCTTTG GCGTTCTCGC GGAAGGTCAG CTTGTGGGTT TCAATCTTTT TATTTCCTCC GCCAGGGACG TGGGTGATAT TGTCCAGGGA CCCAATCTTC GACTGGACTC TGTCCTTGAA GTCAAGCTTC TCAGATTTTA CTTCCACCTG GCCACCTCCT GGTTTATGAT GGATGTTGCC TAATGAGCCA CACTTGGAGG TCACCTTGCT CAGGTCAACT GGTTTGTAGA CTATTTGCAC ACTGCCGCCT CCCGAGACGT GTTTGATATT ATCCTTTGAG CCACACTTGG ACTGGACGTT GCTAAGATCC AGCTTCTTAT TAATTATCTG CACCTTCCCG CCTCCCGGCT GGTGCTTCAG GTTCTCAGTG GAGCCGATCT TGGACTTGAC ATTCTTCAGG TCTGGCATGG GCACGGGGGC TGTCTGCAGG CGGCTCTTGG CGGAAGACGG CGACTTGGGT GGAGTACGGA CCACTGCCAC CTTCTTGGGC TCCCGGGTGG GTGGGGTTGG AAGGGACGGG GTGCGGGAGC GGCTGCCGGG AGTGCCTGGG GAGCCGGGGC TGCTGTAGCC GCTGCGATCC CCTGATTTTG GAGGTTCACC AGAGCTGGGT GGTGTCTTTG GAGCGGGCGG GGTTTTTGCT GGAATCCTGG TGGCGTTGGC CTGGCCCTTC TGGCCTGGAG GGGCTGCTCC CCGCGGTGTG GCGATCTTCG TTTTACCATC AGCCCCCTTG GCTTTTTTGT CATCGCTTCC AGTCCCGTCT TTGCTTTTAC TGACCATGCG AGCTTGGGTC ACGTGACCAG CAGCTTCGTC TTCCAGGCTG GGGGTGTCTC CAATGCCTGC TTCTTCAGCT TTCAGGCCAG CGTCCGTGTC ACCCTCTTGG TCTTGGTGCA TGGTGTAGCC CCCCTGATCT TTCCTGTCCC CCAACCCGTA CGTCCCAGCG TGATCTTCCA TCACTTCGAA CTCCTGGCGG GGCTCAGCCA TGGTGGCGAA TTCTTTACGA GGGTAGGAAG TGGTACGGAA AGTTGGTATA AGACAAAAGT GTTGTGGAAT TGAAGTTTAC TCAAAAAATC AGCACTCTTT TATAGGCGCC CTGGTTTACA TAAGCAAAGC TTATACGTTC TCTATCACTG ATAGGGAGTA AACTGGATAT ACGTTCTCTA TCACTGATAG GGAGTAAACT GTAGATACGT TCTCTATCAC TGATAGGGAG TAAACTGGTC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCCTTATAC GTTCTCTATC ACTGATAGGG AGTAAAGTCT GCATACGTTC TCTATCACTG ATAGGGAGTA AACTCTTCAT ACGTTCTCTA TCACTGATAG GGAGTAAACT CGAGGTGATA ATTCCACGGG GTTGGGGTTG CGCCTTTTCC AAGGCAGCCC TGGGTTTGCG CAGGGACGCG GCTGCTCTGG GCGTGGTTCC GGGAAACGCA GCGGCGCCGA CCCTGGGTCT CGCACATTCT TCACGTCCGT TCGCAGCGTC ACCCGGATCT TCGCCGCTAC CCTTGTGGGC CCCCCGGCGA CGCTTCCTGC TCCGCCCCTA AGTCGGGAAG GTTCCTTGCG GTTCGCGGCG TGCCGGACGT GACAAACGGA AGCCGCACGT CTCACTAGTA CCCTCGCAGA CGGACAGCGC CAGGGAGCAA TGGCAGCGCG CCGACCGCGA TGGGCTGTGG CCAATAGCGG CTGCTCAGCA GGGCGCGCCG AGAGCAGCGG CCGGGAAGGG GCGGTGCGGG AGGCGGGGTG TGGGGCGGTA GTGTGGGCCC TGTTCCTGCC CGCGCGGTGT TCCGCATTCT GCAAGCCTCC GGAGCGCACG TCGGCAGTCG GCTCCCTCGT TGACCGAATC ACCGACCTCT CTCCCCAGGG GGATCATCGA ATTACCATGT CTAGACTGGA CAAGAGCAAA GTCATAAACT CTGCTCTGGA ATTACTCAAT GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC AAAAGCTGGG AGTTGAGCAG CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC TCGATGCCCT GCCAATCGAG ATGCTGGACA GGCATCATAC CCACTCCTGC CCCCTGGAAG GCGAGTCATG GCAAGACTTT CTGCGGAACA ACGCCAAGTC ATACCGCTGT GCTCTCCTCT CACATCGCGA CGGGGCTAAA GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA CCCTGGAAAA TCAGCTCGCG TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT ACGCTCTGTC CGCCGTGGGC CACTTTACAC TGGGCTGCGT ATTGGAGGAA CAGGAGCATC AAGTAGCAAA AGAGGAAAGA GAGACACCTA CCACCGATTC TATGCCCCCA CTTCTGAAAC AAGCAATTGA GCTGTTCGAC CGGCAGGGAG CCGAACCTGC CTTCCTTTTC GGCCTGGAAC TAATCATATG TGGCCTGGAG AAACAGCTAA AGTGCGAAAG CGGCGGGCCG ACCGACGCCC TTGACGATTT TGACTTAGAC ATGCTCCCAG CCGATGCCCT TGACGACTTT GACCTTGATA TGCTGCCTGC TGACGCTCTT GACGATTTTG ACCTTGACAT GCTCCCCGGG TAAACGCGCG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAACGCGAC CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AACGCGTCTG GAACAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAG CTGACGTCCT TTCCATGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG GCCGCCTCCC CGCCTGGAAT TAATTCTGCA GTCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG TTTTTCCAGT CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA AAGAGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGCCTTG ACATTGCTAG CGTTTTACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCAA CTGGATAACT CAAGCTAACC AAAATCATCC CAAACTTCCC ACCCCATACC CTATTACCAC TGCCAATTAC CTAGTGGTTT CATTTACTCT AAACCTGTGA TTCCTCTGAA TTATTTTCAT TTTAAAGAAA TTGTATTTGT TAAATATGTA CTACAAACTT AGTAGTTTTT AAAGAAATTG TATTTGTTAA ATATGTACTA CAAACTTAGT AGT
