pBI121-CFP-End
$113.00
In stock
SKU
PL66272
核内体定位
100μg liquid plasmid(Instantly):$149
| name | pBI121-CFP-End | another name | apBI121-CFP-End |
| Insert Size (bp) | Plasmid host | Plant | |
| Plasmid uses | Subcellular localization | Fragment type | ORF |
| Fragment species | Arabidopsis | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | cyan |
| Promoter | 35s | Replicon | oriV |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | low | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
TGAGCGTCGC AAAGGCGCTC GGTCTTGCCT TGCTCGTCGG TGATGTACTT CACCAGCTCC GCGAAGTCGC TCTTCTTGAT GGAGCGCATG GGGACGTGCT TGGCAATCAC GCGCACCCCC CGGCCGTTTT AGCGGCTAAA AAAGTCATGG CTCTGCCCTC GGGCGGACCA CGCCCATCAT GACCTTGCCA AGCTCGTCCT GCTTCTCTTC GATCTTCGCC AGCAGGGCGA GGATCGTGGC ATCACCGAAC CGCGCCGTGC GCGGGTCGTC GGTGAGCCAG AGTTTCAGCA GGCCGCCCAG GCGGCCCAGG TCGCCATTGA TGCGGGCCAG CTCGCGGACG TGCTCATAGT CCACGACGCC CGTGATTTTG TAGCCCTGGC CGACGGCCAG CAGGTAGGCC GACAGGCTCA TGCCGGCCGC CGCCGCCTTT TCCTCAATCG CTCTTCGTTC GTCTGGAAGG CAGTACACCT TGATAGGTGG GCTGCCCTTC CTGGTTGGCT TGGTTTCATC AGCCATCCGC TTGCCCTCAT CTGTTACGCC GGCGGTAGCC GGCCAGCCTC GCAGAGCAGG ATTCCCGTTG AGCACCGCCA GGTGCGAATA AGGGACAGTG AAGAAGGAAC ACCCGCTCGC GGGTGGGCCT ACTTCACCTA TCCTGCCCGG CTGACGCCGT TGGATACACC AAGGAAAGTC TACACGAACC CTTTGGCAAA ATCCTGTATA TCGTGCGAAA AAGGATGGAT ATACCGAAAA AATCGCTATA ATGACCCCGA AGCAGGGTTA TGCAGCGGAA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCAGA AGGCCGCCAG AGAGGCCGAG CGCGGCCGTG AGGCTTGGAC GCTAGGGCAG GGCATGAAAA AGCCCGTAGC GGGCTGCTAC GGGCGTCTGA CGCGGTGGAA AGGGGGAGGG GATGTTGTCT ACATGGCTCT GCTGTAGTGA GTGGGTTGCG CTCCGGCAGC GGTCCTGATC AATCGTCACC CTTTCTCGGT CCTTCAACGT TCCTGACAAC GAGCCTCCTT TTCGCCAATC CATCGACAAT CACCGCGAGT CCCTGCTCGA ACGCTGCGTC CGGACCGGCT TCGTCGAAGG CGTCTATCGC GGCCCGCAAC AGCGGCGAGA GCGGAGCCTG TTCAACGGTG CCGCCGCGCT CGCCGGCATC GCTGTCGCCG GCCTGCTCCT CAAGCACGGC CCCAACAGTG AAGTAGCTGA TTGTCATCAG CGCATTGACG GCGTCCCCGG CCGAAAAACC CGCCTCGCAG AGGAAGCGAA GCTGCGCGTC GGCCGTTTCC ATCTGCGGTG CGCCCGGTCG CGTGCCGGCA TGGATGCGCG CGCCATCGCG GTAGGCGAGC AGCGCCTGCC TGAAGCTGCG GGCATTCCCG ATCAGAAATG AGCGCCAGTC GTCGTCGGCT CTCGGCACCG AATGCGTATG ATTCTCCGCC AGCATGGCTT CGGCCAGTGC GTCGAGCAGC GCCCGCTTGT TCCTGAAGTG CCAGTAAAGC GCCGGCTGCT GAACCCCCAA CCGTTCCGCC AGTTTGCGTG TCGTCAGACC GTCTACGCCG ACCTCGTTCA ACAGGTCCAG GGCGGCACGG ATCACTGTAT TCGGCTGCAA CTTTGTCATG CTTGACACTT TATCACTGAT AAACATAATA TGTCCACCAA CTTATCAGTG ATAAAGAATC CGCGCGTTCA ATCGGACCAG CGGAGGCTGG TCCGGAGGCC AGACGTGAAA CCCAACATAC CCCTGATCGT AATTCTGAGC ACTGTCGCGC TCGACGCTGT CGGCATCGGC CTGATTATGC CGGTGCTGCC GGGCCTCCTG CGCGATCTGG TTCACTCGAA CGACGTCACC GCCCACTATG GCATTCTGCT GGCGCTGTAT GCGTTGGTGC AATTTGCCTG CGCACCTGTG CTGGGCGCGC TGTCGGATCG TTTCGGGCGG CGGCCAATCT TGCTCGTCTC GCTGGCCGGC GCCAGATCTG GGGAACCCTG TGGTTGGCAT GCACATACAA ATGGACGAAC GGATAAACCT TTTCACGCCC TTTTAAATAT CCGATTATTC TAATAAACGC TCTTTTCTCT TAGGTTTACC CGCCAATATA TCCTGTCAAA CACTGATAGT TTAAACTGAA GGCGGGAAAC GACAATCTGA TCATGAGCGG AGAATTAAGG GAGTCACGTT ATGACCCCCG CCGATGACGC GGGACAAGCC GTTTTACGTT TGGAACTGAC AGAACCGCAA CGTTGAAGGA GCCACTCAGC CGCGGGTTTC TGGAGTTTAA TGAGCTAAGC ACATACGTCA GAAACCATTA TTGCGCGTTC AAAAGTCGCC TAAGGTCACT ATCAGCTAGC AAATATTTCT TGTCAAAAAT GCTCCACTGA CGTTCCATAA ATTCCCCTCG GTATCCAATT AGAGTCTCAT ATTCACTCTC AATCCAAATA ATCTGCACCG GATCTGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GATGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACGGGATCTC TGCGGAACAG GCGGTCGAAG GTGCCGATAT CATTACGACA GCAACGGCCG ACAAGCACAA CGCCACGATC CTGAGCGACA ATATGATCGG GCCCGGCGTC CACATCAACG GCGTCGGCGG CGACTGCCCA GGCAAGACCG AGATGCACCG CGATATCTTG CTGCGTTCGG ATATTTTCGT GGAGTTCCCG CCACAGACCC GGATGATCCC CGATCGTTCA AACATTTGGC AATAAAGTTT CTTAAGATTG AATCCTGTTG CCGGTCTTGC GATGATTATC ATATAATTTC TGTTGAATTA CGTTAAGCAT GTAATAATTA ACATGTAATG CATGACGTTA TTTATGAGAT GGGTTTTTAT GATTAGAGTC CCGCAATTAT ACATTTAATA CGCGATAGAA AACAAAATAT AGCGCGCAAA CTAGGATAAA TTATCGCGCG CGGTGTCATC TATGTTACTA GATCGGGCCT CCTGTCAATG CTGGCGGCGG CTCTGGTGGT GGTTCTGGTG GCGGCTCTGA GGGTGGTGGC TCTGAGGGTG GCGGTTCTGA GGGTGGCGGC TCTGAGGGAG GCGGTTCCGG TGGTGGCTCT GGTTCCGGTG ATTTTGATTA TGAAAAGATG GCAAACGCTA ATAAGGGGGC TATGACCGAA AATGCCGATG AAAACGCGCT ACAGTCTGAC GCTAAAGGCA AACTTGATTC TGTCGCTACT GATTACGGTG CTGCTATCGA TGGTTTCATT GGTGACGTTT CCGGCCTTGC TAATGGTAAT GGTGCTACTG GTGATTTTGC TGGCTCTAAT TCCCAAATGG CTCAAGTCGG TGACGGTGAT AATTCACCTT TAATGAATAA TTTCCGTCAA TATTTACCTT CCCTCCCTCA ATCGGTTGAA TGTCGCCCTT TTGTCTTTGG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA AGCTTGCATG CCTGCAGGTC CCCAGATTAG CCTTTTCAAT TTCAGAAAGA ATGCTAACCC ACAGATGGTT AGAGAGGCTT ACGCAGCAGG TCTCATCAAG ACGATCTACC CGAGCAATAA TCTCCAGGAA ATCAAATACC TTCCCAAGAA GGTTAAAGAT GCAGTCAAAA GATTCAGGAC TAACTGCATC AAGAACACAG AGAAAGATAT ATTTCTCAAG ATCAGAAGTA CTATTCCAGT ATGGACGATT CAAGGCTTGC TTCACAAACC AAGGCAAGTA ATAGAGATTG GAGTCTCTAA AAAGGTAGTT CCCACTGAAT CAAAGGCCAT GGAGTCAAAG ATTCAAATAG AGGACCTAAC AGAACTCGCC GTAAAGACTG GCGAACAGTT CATACAGAGT CTCTTACGAC TCAATGACAA GAAGAAAATC TTCGTCAACA TGGTGGAGCA CGACACACTT GTCTACTCCA AAAATATCAA AGATACAGTC TCAGAAGACC AAAGGGCAAT TGAGACTTTT CAACAAAGGG TAATATCCGG AAACCTCCTC GGATTCCATT GCCCAGCTAT CTGTCACTTT ATTGTGAAGA TAGTGGAAAA GGAAGGTGGC TCCTACAAAT GCCATCATTG CGATAAAGGA AAGGCCATCG TTGAAGATGC CTCTGCCGAC AGTGGTCCCA AAGATGGACC CCCACCCACG AGGAGCATCG TGGAAAAAGA AGACGTTCCA ACCACGTCTT CAAAGCAAGT GGATTGATGT GATATCTCCA CTGACGTAAG GGATGACGCA CAATCCCACT ATCCTTCGCA AGACCCTTCC TCTATATAAG GAAGTTCATT TCATTTGGAG AGAACACGGG GGACTCTAGA GGATCCCCGG GGGTACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAGGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTGGGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGTAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACATCAG CCACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCC ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGCAACTC GAGATGGCTA GCTCTGGAAA CAAGAACATC AACGCCAAAT TGGTATTACT AGGAGATGTT GGAGCTGGAA AATCAAGTCT TGTGCTACGG TTTGTGAAAG ATCAGTTTGT TGAATTTCAG GAATCAACCA TTGGTGCAGC TTTTTTCTCT CAAACATTGG CTGTGAATGA TGCGACTGTG AAGTTTGAGA TATGGGATAC AGCTGGTCAG GAACGATACC ACAGTTTGGC TCCAATGTAC TACAGGGGTG CAGCTGCTGC TATTATTGTC TTTGACATTA CTAATCAAGC CTCATTTGAG AGGGCGAAGA AATGGGTTCA GGAACTGCAG GCACAAGGTA ACCCTAATAT GGTGATGGCT CTTGCTGGAA ACAAAGCTGA TTTATTAGAC GCAAGGAAGG TGTCTGCAGA GGAGGCAGAG ATATATGCTC AAGAGAACAG CCTTTTCTTT ATGGAAACCT CAGCGAAGAC CGCAACAAAT GTCAAAGACA TATTCTACGA AATAGCGAAA AGGCTACCAC GTGTACAGCC AGCTGAAAAC CCGACAGGAA TGGTTCTCCC AAACGGGCCA GGGGCTACGG CAGTGAGTTC ATCGTGTTGT GCTTAGGAGC TCGAATTTCC CCGATCGTTC AAACATTTGG CAATAAAGTT TCTTAAGATT GAATCCTGTT GCCGGTCTTG CGATGATTAT CATATAATTT CTGTTGAATT ACGTTAAGCA TGTAATAATT AACATGTAAT GCATGACGTT ATTTATGAGA TGGGTTTTTA TGATTAGAGT CCCGCAATTA TACATTTAAT ACGCGATAGA AAACAAAATA TAGCGCGCAA ACTAGGATAA ATTATCGCGC GCGGTGTCAT CTATGTTACT AGATCGGGAA TTCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTTG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGGCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGAACCACC ATCAAACAGG ATTTTCGCCT GCTGGGGCAA ACCAGCGTGG ACCGCTTGCT GCAACTCTCT CAGGGCCAGG CGGTGAAGGG CAATCAGCTG TTGCCCGTCT CACTGGTGAA AAGAAAAACC ACCCCAGTAC ATTAAAAACG TCCGCAATGT GTTATTAAGT TGTCTAAGCG TCAATTTGTT TACACCACAA TATATCCTGC CACCAGCCAG CCAACAGCTC CCCGACCGGC AGCTCGGCAC AAAATCACCA CTCGATACAG GCAGCCCATC AGTCCGGGAC GGCGTCAGCG GGAGAGCCGT TGTAAGGCGG CAGACTTTGC TCATGTTACC GATGCTATTC GGAAGAACGG CAACTAAGCT GCCGGGTTTG AAACACGGAT GATCTCGCGG AGGGTAGCAT GTTGATTGTA ACGATGACAG AGCGTTGCTG CCTGTGATCA AATATCATCT CCCTCGCAGA GATCCGAATT ATCAGCCTTC TTATTCATTT CTCGCTTAAC CGTGACAGGC TGTCGATCTT GAGAACTATG CCGACATAAT AGGAAATCGC TGGATAAAGC CGCTGAGGAA GCTGAGTGGC GCTATTTCTT TAGAAGTGAA CGTTGACGAT ATCAACTCCC CTATCCATTG CTCACCGAAT GGTACAGGTC GGGGACCCGA AGTTCCGACT GTCGGCCTGA TGCATCCCCG GCTGATCGAC CCCAGATCTG GGGCTGAGAA AGCCCAGTAA GGAAACAACT GTAGGTTCGA GTCGCGAGAT CCCCCGGAAC CAAAGGAAGT AGGTTAAACC CGCTCCGATC AGGCCGAGCC ACGCCAGGCC GAGAACATTG GTTCCTGTAG GCATCGGGAT TGGCGGATCA AACACTAAAG CTACTGGAAC GAGCAGAAGT CCTCCGGCCG CCAGTTGCCA GGCGGTAAAG GTGAGCAGAG GCACGGGAGG TTGCCACTTG CGGGTCAGCA CGGTTCCGAA CGCCATGGAA ACCGCCCCCG CCAGGCCCGC TGCGACGCCG ACAGGATCTA GCGCTGCGTT TGGTGTCAAC ACCAACAGCG CCACGCCCGC AGTTCCGCAA ATAGCCCCCA GGACCGCCAT CAATCGTATC GGGCTACCTA GCAGAGCGGC AGAGATGAAC ACGACCATCA GCGGCTGCAC AGCGCCTACC GTCGCCGCGA CCCCGCCCGG CAGGCGGTAG ACCGAAATAA ACAACAAGCT CCAGAATAGC GAAATATTAA GTGCGCCGAG GATGAAGATG CGCATCCACC AGATTCCCGT TGGAATCTGT CGGACGATCA TCACGAGCAA TAAACCCGCC GGCAACGCCC GCAGCAGCAT ACCGGCGACC CCTCGGCCTC GCTGTTCGGG CTCCACGAAA ACGCCGGACA GATGCGCCTT GTGAGCGTCC TTGGGGCCGT CCTCCTGTTT GAAGACCGAC AGCCCAATGA TCTCGCCGTC GATGTAGGCG CCGAATGCCA CGGCATCTCG CAACCGTTCA GCGAACGCCT CCATGGGCTT TTTCTCCTCG TGCTCGTAAA CGGACCCGAA CATCTCTGGA GCTTTCTTCA GGGCCGACAA TCGGATCTCG CGGAAATCCT GCACGTCGGC CGCTCCAAGC CGTCGAATCT GAGCCTTAAT CACAATTGTC AATTTTAATC CTCTGTTTAT CGGCAGTTCG TAGAGCGCGC CGTGCGTCCC GAGCGATACT GAGCGAAGCA AGTGCGTCGA GCAGTGCCCG CTTGTTCCTG AAATGCCAGT AAAGCGCTGG CTGCTGAACC CCCAGCCGGA ACTGACCCCA CAAGGCCCTA GCGTTTGCAA TGCACCAGGT CATCATTGAC CCAGGCGTGT TCCACCAGGC CGCTGCCTCG CAACTCTTCG CAGGCTTCGC CGACCTGCTC GCGCCACTTC TTCACGCGGG TGGAATCCGA TCCGCACATG AGGCGGAAGG TTTCCAGCTT GAGCGGGTAC GGCTCCCGGT GCGAGCTGAA ATAGTCGAAC ATCCGTCGGG CCGTCGGCGA CAGCTTGCGG TACTTCTCCC ATATGAATTT CGTGTAGTGG TCGCCAGCAA ACAGCACGAC GATTTCCTCG TCGATCAGGA CCTGGCAACG GGACGTTTTC TTGCCACGGT CCAGGACGCG GAAGCGGTGC AGCAGCGACA CCGATTCCAG GTGCCCAACG CGGTCGGACG TGAAGCCCAT CGCCGTCGCC TGTAGGCGCG ACAGGCATTC CTCGGCCTTC GTGTAATACC GGCCATTGAT CGACCAGCCC AGGTCCTGGC AAAGCTCGTA GAACGTGAAG GTGATCGGCT CGCCGATAGG GGTGCGCTTC GCGTACTCCA ACACCTGCTG CCACACCAGT TCGTCATCGT CGGCCCGCAG CTCGACGCCG GTGTAGGTGA TCTTCACGTC CTTGTTGACG TGGAAAATGA CCTTGTTTTG CAGCGCCTCG CGCGGGATTT TCTTGTTGCG CGTGGTGAAC AGGGCAGAGC GGGCCGTGTC GTTTGGCATC GCTCGCATCG TGTCCGGCCA CGGCGCAATA TCGAACAAGG AAAGCTGCAT TTCCTTGATC TGCTGCTTCG TGTGTTTCAG CAACGCGGCC TGCTTGGCCT CGCTGACCTG TTTTGCCAGG TCCTCGCCGG CGGTTTTTCG CTTCTTGGTC GTCATAGTTC CTCGCGTGTC GATGGTCATC GACTTCGCCA AACCTGCCGC CTCCTGTTCG AGACGACGCG AACGCTCCAC GGCGGCCGAT GGCGCGGGCA GGGCAGGGGG AGCCAGTTGC ACGCTGTCGC GCTCGATCTT GGCCGTAGCT TGCTGGACCA TCGAGCCGAC GGACTGGAAG GTTTCGCGGG GCGCACGCAT GACGGTGCGG CTTGCGATGG TTTCGGCATC CTCGGCGGAA AACCCCGCGT CGATCAGTTC TTGCCTGTAT GCCTTCCGGT CAAACGTCCG ATTCATTCAC CCTCCTTGCG GGATTGCCCC GACTCACGCC GGGGCAATGT GCCCTTATTC CTGATTTGAC CCGCCTGGTG CCTTGGTGTC CAGATAATCC ACCTTATCGG CAATGAAGTC GGTCCCGTAG ACCGTCTGGC CGTCCTTCTC GTACTTGGTA TTCCGAATCT TGCCCTGCAC GAATACCAGC GACCCCTTGC CCAAATACTT GCCGTGGGCC TCGGCCTGAG AGCCAAAACA CTTGATGCGG AAGAAGTCGG TGCGCTCCTG CTTGTCGCCG GCATCGTTGC GCCACATCTA GGTACTAAAA CAATTCATCC AGTAAAATAT AATATTTTAT TTTCTCCCAA TCAGGCTTGA TCCCCAGTAA GTCAAAAAAT AGCTCGACAT ACTGTTCTTC CCCGATATCC TCCCTGATCG ACCGGACGCA GAAGGCAATG TCATACCACT TGTCCGCCCT GCCGCTTCTC CCAAGATCAA TAAAGCCACT TACTTTGCCA TCTTTCACAA AGATGTTGCT GTCTCCCAGG TCGCCGTGGG AAAAGACAAG TTCCTCTTCG GGCTTTTCCG TCTTTAAAAA ATCATACAGC TCGCGCGGAT CTTTAAATGG AGTGTCTTCT TCCCAGTTTT CGCAATCCAC ATCGGCCAGA TCGTTATTCA GTAAGTAATC CAATTCGGCT AAGCGGCTGT CTAAGCTATT CGTATAGGGA CAATCCGATA TGTCGATGGA GTGAAAGAGC CTGATGCACT CCGCATACAG CTCGATAATC TTTTCAGGGC TTTGTTCATC TTCATACTCT TCCGAGCAAA GGACGCCATC GGCCTCACTC ATGAGCAGAT TGCTCCAGCC ATCATGCCGT TCAAAGTGCA GGACCTTTGG AACAGGCAGC TTTCCTTCCA GCCATAGCAT CATGTCCTTT TCCCGTTCCA CATCATAGGT GGTCCCTTTA TACCGGCTGT CCGTCATTTT TAAATATAGG TTTTCATTTT CTCCCACCAG CTTATATACC TTAGCAGGAG ACATTCCTTC CGTATCTTTT ACGCAGCGGT ATTTTTCGAT CAGTTTTTTC AATTCCGGTG ATATTCTCAT TTTAGCCATT TATTATTTCC TTCCTCTTTT CTACAGTATT TAAAGATACC CCAAGAAGCT AATTATAACA AGACGAACTC CAATTCACTG TTCCTTGCAT TCTAAAACCT TAAATACCAG AAAACAGCTT TTTCAAAGTT GTTTTCAAAG TTGGCGTATA ACATAGTATC GACGGAGCCG ATTTTGAAAC CACAATTATG GGTGATGCTG CCAACTTACT GATTTAGTGT ATGATGGTGT TTTTGAGGTG CTCCAGTGGC TTCTGTGTCT ATCAGCTGTC CCTCCTGTTC AGCTACTGAC GGGGTGGTGC GTAACGGCAA AAGCACCGCC GGACATCAGC GCTATCTCTG CTCTCACTGC CGTAAAACAT GGCAACTGCA GTTCACTTAC ACCGCTTCTC AACCCGGTAC GCACCAGAAA ATCATTGATA TGGCCATGAA TGGCGTTGGA TGCCGGGCAA CAGCCCGCAT TATGGGCGTT GGCCTCAACA CGATTTTACG TCACTTAAAA AACTCAGGCC GCAGTCGGTA ACCTCGCGCA TACAGCCGGG CAGTGACGTC ATCGTCTGCG CGGAAATGGA CGAACAGTGG GGCTATGTCG GGGCTAAATC GCGCCAGCGC TGGCTGTTTT ACGCGTATGA CAGTCTCCGG AAGACGGTTG TTGCGCACGT ATTCGGTGAA CGCACTATGG CGACGCTGGG GCGTCTTATG AGCCTGCTGT CACCCTTTGA CGTGGTGATA TGGATGACGG ATGGCTGGCC GCTGTATGAA TCCCGCCTGA AGGGAAAGCT GCACGTAATC AGCAAGCGAT ATACGCAGCG AATTGAGCGG CATAACCTGA ATCTGAGGCA GCACCTGGCA CGGCTGGGAC GGAAGTCGCT GTCGTTCTCA AAATCGGTGG AGCTGCATGA CAAAGTCATC GGGCATTATC TGAACATAAA ACACTATCAA TAAGTTGGAG TCATTACCCA ATTATGATAG AATTTACAAG CTATAAGGTT ATTGTCCTGG GTTTCAAGCA TTAGTCCATG CAAGTTTTTA TGCTTTGCCC ATTCTATAGA TATATTGATA AGCGCGCTGC CTATGCCTTG CCCCCTGAAA TCCTTACATA CGGCGATATC TTCTATATAA AAGATATATT ATCTTATCAG TATTGTCAAT ATATTCAAGG CAATCTGCCT CCTCATCCTC TTCATCCTCT TCGTCTTGGT AGCTTTTTAA ATATGGCGCT TCATAGAGTA ATTCTGTAAA GGTCCAATTC TCGTTTTCAT ACCTCGGTAT AATCTTACCT ATCACCTCAA ATGGTTCGCT GGGTTTATCG CACCCCCGAA CACGAGCACG GCACCCGCGA CCACTATGCC AAGAATGCCC AAGGTAAAAA TTGCCGGCCC CGCCATGAAG TCCGTGAATG CCCCGACGGC CGAAGTGAAG GGCAGGCCGC CACCCAGGCC GCCGCCCTCA CTGCCCGGCA CCTGGTCGCT GAATGTCGAT GCCAGCACCT GCGGCACGTC AATGCTTCCG GGCGTCGCGC TCGGGCTGAT CGCCCATCCC GTTACTGCCC CGATCCCGGC AATGGCAAGG ACTGCCAGCG CTGCCATTTT TGGGGTGAGG CCGTTCGCGG CCGAGGGGCG CAGCCCCTGG GGGGATGGGA GGCCCGCGTT AGCGGGCCGG GAGGGTTCGA GAAGGGGGGG CACCCCCCTT CGGCGTGCGC GGTCACGCGC ACAGGGCGCA GCCCTGGTTA AAAACAAGGT TTATAAATAT TGGTTTAAAA GCAGGTTAAA AGACAGGTTA GCGGTGGCCG AAAAACGGGC GGAAACCCTT GCAAATGCTG GATTTTCTGC CTGTGGACAG CCCCTCAAAT GTCAATAGGT GCGCCCCTCA TCTGTCAGCA CTCTGCCCCT CAAGTGTCAA GGATCGCGCC CCTCATCTGT CAGTAGTCGC GCCCCTCAAG TGTCAATACC GCAGGGCACT TATCCCCAGG CTTGTCCACA TCATCTGTGG GAAACTCGCG TAAAATCAGG CGTTTTCGCC GATTTGCGAG GCTGGCCAGC TCCACGTCGC CGGCCGAAAT CGAGCCTGCC CCTCATCTGT CAACGCCGCG CCGGGTGAGT CGGCCCCTCA AGTGTCAACG TCCGCCCCTC ATCTGTCAGT GAGGGCCAAG TTTTCCGCGA GGTATCCACA ACGCCGGCGG CCGCGGTGTC TCGCACACGG CTTCGACGGC GTTTCTGGCG CGTTTGCAGG GCCATAGACG GCCGCCAGCC CAGCGGCGAG GGCAACCAGC CCGG
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