pUC-GGox(Aspergillus+niger)-GFP-AT
$70.00
In stock
SKU
PL65688
MH593586.1
100μg liquid plasmid(Instantly):$91
| name | pUC-GGox(Aspergillus+niger)-GFP-AT | another name | apUC-GGox(Aspergillus+niger)-GFP-AT |
| Insert Size (bp) | 1818bp | Plasmid host | Escherichia coli |
| Plasmid uses | Gene template | Fragment type | cDNA |
| Fragment species | Aspergillus niger | Prokaryotic resistance | Kan |
| Selective marker | Fluorescent labeling | ||
| Promoter | Replicon | pUC | |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
ATCGAATCTC GTCAGCTATC GTCAGCTTAC CTTTTTGGCA GCGATCGCGG CTCCCGACAT CTTGGACCAT TAGCTCCACA GGTATCTTCT TCCCTCTAGT GGTCATAACA GCAGCTTCAG CTACCTCTCA ATTCAAAAAA CCCCTCAAGA CCCGTTTAGA GGCCCCAAGG GGTTATGCTA TCAATCGTTG CGTTACACAC ACAAAAAACC AACACACATC CATCTTCGAT GGATAGCGAT TTTATTATCT AACTGCTGAT CGAGTGTAGC CAGATCTTTG TCGATCCTAC CATCCACTCG ACACACCCGC CAGCGGCCGC TGCCAAGCTT CCGAGCTCAT GCAGACTCTC CTTGTGAGCT CGCTTGTGGT CTCCCTCGCT GCAGCCCTCC CACACTACAT CAGGAGCAAT GGCATTGAAG CCAGCCTCCT GACTGACCCC AAGGATGTTA CCGGCCGCAC TGTCGACTAC ATCATCGCTG GTGGAGGTCT GACTGGACTC ACTACTGCTG CCCGTCTGAC GGAGAACCCC AATATCACTG TGCTTGTCAT CGAAAGTGGC TCCTACGAGT CTGACAGAGG TCCTATCATT GAGGACCTGA ACGCTTACGG TGACATTTTT GGCAGCAGTG TGGACCACGC CTACGAGACT GTCGAGCTCG CCACCAACAA TCAGACTGCG CTAATCCGCT CCGGAAATGG TCTCGGTGGC TCTACCCTCG TCAACGGTGG CACCTGGACT CGCCCCCACA AGGCACAAGT TGACTCGTGG GAGACCGTCT TCGGAAATGA GGGCTGGAAC TGGGACAGCG TGGCCGCCTA CTCCCTCCAG GCTGAGCGTG CTCGCGCACC AAATGCCAAA CAGATTGCTG CTGGCCACTA CTTTAATGCA TCCTGCCATG GTCTCAATGG TACTGTCCAC GCCGGACCCC GCGATACCGG TGATGACTAC TCCCCCATCG TCAAGGCTCT CATGAGCGTT GTCGAAGACA GGGGCGTCCC CACCAAGAAG GACTTGGGAT GCGGTGACCC CCATGGTGTG TCCATGTTCC CCAACACCTT GCACGAAGAC CAAGTGCGCT CTGATGCCGC TCGCGAATGG CTCCTCCCCA ACTACCAGCG GCCCAACCTG CAAGTCCTCA CTGGACAGTA TGTTGGAAAG GTTCTGCTCA GCCAGAACGC TACCACACCT CGTGCCGTTG GCGTGGAATT CGGCACCCAC AAGGGCAACA CCCACAACGT CTACGCTAAG CACGAGGTCC TCCTAGCCGC TGGATCCGCG GTCTCTCCCA CCATCCTCGA ATATTCCGGT ATCGGAATGA AGTCCATTCT GGAGCCTCTT GGAATTGACA CCGTCGTTGA CCTGCCCGTC GGTCTCAACC TTCAGGACCA GACCACCTCT ACCGTCCGCT CACGCATTAC CTCCGCCGGT GCCGGACAGG GACAGGCCGC TTGGTTCGCT ACCTTCAACG AGACCTTTGG CGACTACACC GAAAAGGCCC ACGAGCTGCT CAACACCAAG CTGGAGCAGT GGGCCGAAGA GGCCGTCGCC CGTGGCGGAT TCCACAACAC CACCGCTTTG CTCATCCAGT ACGAGAACTA CCGCGACTGG ATCGTCAAGG ACAATGTCGC ATACTCGGAA CTCTTCCTCG ACACGGCCGG AGTGGCCAGT TTCGATGTGT GGGATCTTCT GCCCTTCACT AGAGGATACG TCCACATCCT CGACAAGGAC CCCTACCTCC GCCATTTCGC CTACGACCCT CAGTACTTCC TCAACGAGCT TGACCTGCTC GGCCAGGCTG CCGCCACTCA GCTGGCCCGC AACATCTCCA ACTCCGGTGC CATGCAAACT TATTTCGCTG GAGAGACTAT TCCCGGTGAC AACCTCGCGT ATGATGCCGA CTTGAGCGCC TGGGTTGAGT ATATCCCGTA CAACTTCCGC CCTAACTACC ATGGTGTGGG TACTTGCTCC ATGATGCCGA AGGAGATGGG CGGTGTTGTC GACAATGCTG CCCGTGTGTA TGGTGTGCAG GGACTGCGAG TCATCGATGG TTCTATTCCC CCTACGCAAA TGTCGTCCCA TGTTATGACG GTCTTTTATG CCATGGCCTT GAAGATTGCG GATGCCGTCT TGGCGGATTA TGCTTCCATG CAGTGACTCG AGGTTTCTAG AAGTTGTCTC CTCCTGCACT GACTGACTGA TACAATCGAT TTCTGGATCC GCAGGCCTCT GCTAGCTTGA CTGACTGAGA TACAGCGTAC CTTCAGCTCA CAGACATGAT AAGATACATT GATGAGTTTG GACAAACCAC AACTAGAATG CAGTGAAAAA AATGCTTTAT TTGTGAAATT TGTGATGCTA TTGCTTTATT TGTAACCATT ATAAGCTGCA ATAAACAAGT TAACAACAAC AATTGCATTC ATTTTATGTT TCAGGTTCAG GGGGAGGTGT GGGAGGTTTT TTAAAGCAAG TAAAACCTCT ACAAATGTGG TATTGGCCCA TCTCTATCGG TATCGTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGTGCCCCTC GGGCCGGATT GCTATCTACC GGCATTGGCG CAGAAAAAAA TGCCTGATGC GACGCTGCGC GTCTTATACT CCCACATATG CCAGATTCAG CAACGGATAC GGCTTCCCCA ACTTGCCCAC TTCCATACGT GTCCTCCTTA CCAGAAATTT ATCCTTAAGG TCGTCAGCTA TCCTGCAGGC GATCTCTCGA TTTCGATCAA GACATTCCTT TAATGGTCTT TTCTGGACAC CACTAGGGGT CAGAAGTAGT TCATCAAACT TTCTTCCCTC CCTAATCTCA TTGGTTACCT TGGGCTATCG AAACTTAATT AACCAGTCAA GTCAGCTACT TGGCGAGATC GACTTGTCTG GGTTTCGACT ACGCTCAGAA TTGCGTCAGT CAAGTTCGAT CTGGTCCTTG CTATTGCACC CGTTCTCCGA TTACGAGTTT CATTTAAATC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCATTTAA ATTTCCGAAC TCTCCAAGGC CCTCGTCGGA AAATCTTCAA ACCTTTCGTC CGATCCATCT TGCAGGCTAC CTCTCGAACG AACTATCGCA AGTCTCTTGG CCGGCCTTGC GCCTTGGCTA TTGCTTGGCA GCGCCTATCG CCAGGTATTA CTCCAATCCC GAATATCCGA GATCGGGATC ACCCGAGAGA AGTTCAACCT ACATCCTCAA TCCCGATCTA TCCGAGATCC GAGGAATATC GAAATCGGGG CGCGCCAAGC TGTTGTGACC GCTTGCTCTA GCCAGCTATC GAGTTGTGAA CCGATCCATC TAGCAATTGG TCTCGATCTA GCGATAGGCT TCGATCTAGC TATGTAGAAA CGCCGTGTGC TCGATCGCTT GATAAGGTCC ACGTAGCTGC TATAATTGCT TCAACAGAAC ATATTGACTA TCCGGTATTA CCCGGCAAAG GAGGTAGCCA ACATGACAGC TCTGACAGAG GGAGCGAAAC TGTTCGAGAA GGAGATCCCG TACATCACAG AGTTGGAAGG AGATGTCGAA GGGATGAAAT TCATCATCAA GGGAGAAGGA ACGGGTGACG CAACGACCGG AACGATCAAG GCCAAATACA TTTGTACCAC GGGCGATTTG CCTGTCCCCT GGGCGACGCT TGTAAGCACG CTCTCGTATG GTGTCCAGTG CTTCGCGAAA TATCCATCGC ACATTAAGGA CTTTTTCAAG TCGGCCATGC CAGAAGGTTA CACACAAGAA CGAACCATCT CCTTTGAGGG GGACGGAGTG TATAAGACAC GCGCGATGGT AACGTACGAG CGCGGGTCCA TCTACAACAG GGTAACTCTT ACTGGGGAGA ACTTTAAGAA GGACGGGCAT ATCTTGCGGA AAAACGTGGC ATTTCAATGT CCGCCCTCGA TTCTGTATAT TCTCCCGGAC ACGGTGAATA ATGGGATCAG AGTGGAGTTC AACCAGGCAT ACGATATTGA GGGTGTGACT GAAAAGCTCG TCACCAAATG CAGCCAGATG AATCGCCCCC TTGCGGGATC AGCAGCCGTC CATATCCCCC GGTATCACCA TATTACCTAC CACACAAAAC TCTCAAAAGA CAGAGATGAG AGAAGGGACC ATATGTGCCT GGTCGAAGTA GTGAAGGCGG TGGATCTTGA TACATACCAG TAGGCTAGCC ATTGGCGCAG AAAAAAATGC CTGATGCGAC GCTGCGCGTC TTATACTCCC ACATATGCCA GATTCAGCAA CGGATACGGC TTCCCCAACT TGCCCACTTC CATACGTGTC CTCCTTACCA GAAATTTATC CTTAAGGGCG CGCCTGGTGT ACCGAGAACG ATCCTCTCAG TGCGAGTCTC GACGATCCAT ATCGTTGCTT GGCAGTCAGC CAGTCGGAAT CCAGCTTGGG ACCCAGGAAG TCCAATCGTC AGATATTGTA CTCAAGCCTG GTCACGGCAG CGTACCGATC TGTTTAAACC TAGATATTGA TAGTCTGATC GGTCAACGTA TAATCGAGTC CTAGCTTTTG CAAACATCTA TCAAGAGACA GGATCAGCAG GAGGCTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCG GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGTCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCGACGACG GGCGTTCCTT GCGCGGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGT CTATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCACGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGTCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTTGTGCTT TACGGTATCG CCGCGCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGACCGA TTCTAGGTGC ATTGGCGCAG AAAAAAATGC CTGATGCGAC GCTGCGCGTC TTATACTCCC ACATATGCCA GATTCAGCAA CGGATACGGC TTCCCCAACT TGCCCACTTC CATACGTGTC CTCCTTACCA GAAATTTATC CTTAAGGTCG TTTAAACTCG ACTCTGGCTC TATCGAATCT CCGTCGTTTC GAGCTTACGC GAACAGCCGT GGCGCTCATT TGCTCGTCGG GC
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