pCMV-USP13(human)-FLAG
$84.00
In stock
SKU
PL65374
NM_003940.3;IsoT-3; ISOT3
100μg liquid plasmid(Instantly):$105
| name | pCMV-USP13(human)-FLAG | another name | apCMV-USP13(human)-FLAG |
| Insert Size (bp) | 2592bp | Plasmid host | Mammary cell |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Kan |
| Selective marker | Neo/G418 | Fluorescent labeling | |
| Promoter | CMV | Replicon | pUC |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
GAGCTCCACC GCGGTGGCGG CCGCGCCACC ATGCAGCGCC GGGGCGCCCT GTTCGGCATG CCGGGCGGCA GCGGAGGCAG GAAGATGGCT GCAGGAGACA TCGGCGAGCT GCTAGTGCCC CACATGCCCA CGATCCGCGT GCCCAGGTCC GGCGACAGGG TCTACAAGAA CGAGTGCGCC TTCTCCTACG ACTCTCCCAA TTCTGAAGGT GGACTCTATG TATGCATGAA TACATTTTTG GCCTTTGGAA GGGAACATGT TGAAAGACAT TTTCGAAAAA CTGGACAGAG TGTATACATG CACCTGAAAA GACATGTGCG AGAGAAGGTA AGAGGGGCGT CTGGTGGAGC GTTACCAAAA AGGAGGAATT CCAAGATTTT TTTAGATCTA GATACTGATG ACGATTTAAA TAGCGACGAT TATGAATATG AAGATGAAGC CAAACTTGTT ATATTCCCAG ATCACTATGA AATAGCACTA CCAAATATTG AGGAGTTACC AGCCCTGGTA ACAATTGCTT GTGATGCAGT TCTCAGCTCA AAATCTCCAT ACAGAAAGCA GGACCCAGAC ACGTGGGAAA ATGAATTGCC AGTATCTAAA TATGCCAACA ACCTCACCCA GCTGGACAAT GGAGTCAGGA TTCCTCCAAG TGGTTGGAAG TGTGCCAGAT GCGACCTGCG AGAAAACCTC TGGTTGAATC TGACTGACGG CTCTGTCCTG TGTGGAAAGT GGTTCTTTGA CAGCTCTGGG GGCAACGGGC ATGCGCTGGA GCATTACAGA GACATGGGCT ACCCACTAGC CGTGAAACTG GGAACCATCA CTCCTGACGG GGCAGATGTT TATTCTTTTC AAGAAGAAGA ACCTGTTTTG GATCCTCATT TGGCCAAGCA CTTAGCGCAT TTTGGAATTG ATATGCTTCA TATGCATGGG ACAGAGAATG GGCTCCAGGA CAATGACATC AAGCTGAGGG TCAGTGAGTG GGAAGTGATC CAGGAGTCGG GCACGAAACT GAAGCCAATG TATGGTCCTG GCTACACGGG TCTGAAGAAC CTGGGCAACA GCTGCTATCT CAGCTCTGTC ATGCAGGCCA TCTTCAGCAT CCCAGAATTC CAGAGAGCGT ATGTAGGAAA CCTTCCCAGA ATATTTGACT ACTCGCCTTT AGATCCAACA CAAGATTTCA ACACACAGAT GACTAAGTTA GGACATGGCC TTCTCTCAGG CCAGTATTCA AAGCCTCCGG TGAAATCTGA ACTCATTGAA CAGGTGATGA AGGAGGAGCA CAAGCCACAG CAGAACGGGA TCTCTCCGCG CATGTTTAAG GCCTTTGTAA GCAAGAGCCA CCCGGAATTC TCCTCTAACA GGCAGCAAGA TGCCCAGGAA TTCTTCTTGC ACCTGGTGAA TCTAGTAGAG AGGAACCGCA TCGGCTCAGA AAACCCAAGC GATGTTTTTC GTTTTTTGGT GGAAGAACGC ATTCAGTGCT GTCAGACCCG GAAAGTCCGC TACACGGAGA GGGTGGATTA CCTGATGCAG TTACCTGTGG CCATGGAGGC GGCAACCAAC AAGGATGAAC TGATCGCTTA TGAACTAACG AGAAGGGAAG CAGAAGCAAA CAGAAGACCC CTTCCTGAGT TGGTACGTGC CAAGATACCA TTTAGTGCCT GCCTTCAGGC CTTCTCTGAA CCAGAAAATG TTGATGATTT CTGGAGCAGT GCCCTACAAG CAAAGTCTGC GGGTGTGAAA ACATCTCGCT TTGCTTCATT CCCTGAATAC TTGGTAGTGC AGATAAAGAA GTTCACTTTT GGTCTTGACT GGGTTCCCAA AAAATTTGAT GTTTCTATTG ATATGCCAGA CCTACTTGAT ATCAACCATC TCCGAGCCAG GGGGTTACAG CCAGGAGAGG AAGAACTTCC AGACATCAGC CCCCCCATAG TCATTCCTGA TGACTCAAAA GATCGCCTGA TGAACCAATT GATAGACCCA TCAGACATCG ATGAGTCATC AGTGATGCAG CTGGCCGAGA TGGGTTTCCC GCTGGAAGCA TGTCGCAAGG CTGTGTACTT CACTGGAAAT ATGGGCGCCG AGGTGGCCTT CAACTGGATC ATTGTTCACA TGGAAGAGCC AGATTTTGCT GAGCCGCTGA CCATGCCTGG TTATGGAGGG GCAGCTTCTG CTGGAGCCTC TGTTTTTGGT GCTTCTGGAC TGGATAACCA ACCTCCAGAG GAAATCGTAG CTATCATCAC CTCCATGGGA TTTCAGCGAA ATCAGGCTAT TCAGGCACTA CGAGCAACGA ATAATAACCT GGAAAGAGCA CTGGATTGGA TCTTTAGCCA CCCTGAGTTT GAAGAAGACA GTGATTTTGT GATTGAGATG GAGAATAATG CCAATGCAAA CATTATTTCT GAGGCCAAGC CCGAAGGACC TAGAGTCAAG GATGGATCTG GAACATATGA GCTATTTGCA TTCATCAGTC ACATGGGAAC ATCCACAATG AGTGGTCATT ACATTTGCCA TATCAAAAAG GAAGGAAGAT GGGTGATTTA CAATGACCAC AAAGTTTGTG CCTCAGAAAG GCCCCCTAAA GACCTGGGCT ACATGTACTT TTACCGCAGG ATACCAAGCC TCGAGTCTAG AGATTACAAG GATGACGACG ATAAGTAAGG GCCCGGTACC TTAATTAATT AAGGTACCAG G
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