pMIGR1-FLAG-BCR-ABL-P210
$157.00
In stock
SKU
PL65340
100μg liquid plasmid(Instantly):$178
| name | pMIGR1-FLAG-BCR-ABL-P210 | another name | apMIGR1-FLAG-BCR-ABL-P210 |
| Insert Size (bp) | 6093bp | Plasmid host | Mammary cell,retrovirus |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Human | Prokaryotic resistance | Amp |
| Selective marker | Fluorescent labeling | Green | |
| Promoter | MESV | Replicon | pUC |
| Competence | Stbl3 | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC TAGGCGCCGG AATTAGATCT CTCGAGGCCA CCATGGATTA CAAGGATGAC GACGATAAGA TGGTGGACCC GGTGGGCTTC GCGGAGGCGT GGAAGGCGCA GTTCCCGGAC TCAGAGCCCC CGCGCATGGA GCTGCGCTCA GTGGGCGACA TCGAGCAGGA GCTGGAGCGC TGCAAGGCCT CCATTCGGCG CCTGGAGCAG GAGGTGAACC AGGAGCGCTT CCGCATGATC TACCTGCAGA CGTTGCTGGC CAAGGAAAAG AAGAGCTATG ACCGGCAGCG ATGGGGCTTC CGGCGCGCGG CGCAGGCCCC CGACGGCGCC TCCGAGCCCC GAGCGTCCGC GTCGCGCCCG CAGCCAGCGC CCGCCGACGG AGCCGACCCG CCGCCCGCCG AGGAGCCCGA GGCCCGGCCC GACGGCGAGG GTTCTCCGGG TAAGGCCAGG CCCGGGACCG CCCGCAGGCC CGGGGCAGCC GCGTCGGGGG AACGGGACGA CCGGGGACCC CCCGCCAGCG TGGCGGCGCT CAGGTCCAAC TTCGAGCGGA TACGCAAGGG CCATGGCCAG CCCGGGGCGG ACGCCGAGAA GCCCTTCTAC GTGAACGTCG AGTTTCACCA CGAGCGCGGC CTGGTGAAGG TCAACGACAA AGAGGTGTCG GACCGCATCA GCTCCCTGGG CAGCCAGGCC ATGCAGATGG AGCGCAAAAA GTCCCAGCAC GGCGCGGGCT CGAGCGTGGG GGATGCATCC AGGCCCCCTT ACCGGGGACG CTCCTCGGAG AGCAGCTGCG GCGTCGACGG CGACTACGAG GACGCCGAGT TGAACCCCCG CTTCCTGAAG GACAACCTGA TCGACGCCAA TGGCGGTAGC AGGCCCCCTT GGCCGCCCCT GGAGTACCAG CCCTACCAGA GCATCTACGT CGGGGGCATG ATGGAAGGGG AGGGCAAGGG CCCGCTCCTG CGCAGCCAGA GCACCTCTGA GCAGGAGAAG CGCCTTACCT GGCCCCGCAG GTCCTACTCC CCCCGGAGTT TTGAGGATTG CGGAGGCGGC TATACCCCGG ACTGCAGCTC CAATGAGAAC CTCACCTCCA GCGAGGAGGA CTTCTCCTCT GGCCAGTCCA GCCGCGTGTC CCCAAGCCCC ACCACCTACC GCATGTTCCG GGACAAAAGC CGCTCTCCCT CGCAGAACTC GCAACAGTCC TTCGACAGCA GCAGTCCCCC CACGCCGCAG TGCCATAAGC GGCACCGGCA CTGCCCGGTT GTCGTGTCCG AGGCCACCAT CGTGGGCGTC CGCAAGACCG GGCAGATCTG GCCCAACGAT GGCGAGGGCG CCTTCCATGG AGACGCAGAT GGCTCGTTCG GAACACCACC TGGATACGGC TGCGCTGCAG ACCGGGCAGA GGAGCAGCGC CGGCACCAAG ATGGGCTGCC CTACATTGAT GACTCGCCCT CCTCATCGCC CCACCTCAGC AGCAAGGGCA GGGGCAGCCG GGATGCGCTG GTCTCGGGAG CCCTGGAGTC CACTAAAGCG AGTGAGCTGG ACTTGGAAAA GGGCTTGGAG ATGAGAAAAT GGGTCCTGTC GGGAATCCTG GCTAGCGAGG AGACTTACCT GAGCCACCTG GAGGCACTGC TGCTGCCCAT GAAGCCTTTG AAAGCCGCTG CCACCACCTC TCAGCCGGTG CTGACGAGTC AGCAGATCGA GACCATCTTC TTCAAAGTGC CTGAGCTCTA CGAGATCCAC AAGGAGTTCT ATGATGGGCT CTTCCCCCGC GTGCAGCAGT GGAGCCACCA GCAGCGGGTG GGCGACCTCT TCCAGAAGCT GGCCAGCCAG CTGGGTGTGT ACCGGGCCTT CGTGGACAAC TACGGAGTTG CCATGGAAAT GGCTGAGAAG TGCTGTCAGG CCAATGCTCA GTTTGCAGAA ATCTCCGAGA ACCTGAGAGC CAGAAGCAAC AAAGATGCCA AGGATCCAAC GACCAAGAAC TCTCTGGAAA CTCTGCTCTA CAAGCCTGTG GACCGTGTGA CGAGGAGCAC GCTGGTCCTC CATGACTTGC TGAAGCACAC TCCTGCCAGC CACCCTGACC ACCCCTTGCT GCAGGACGCC CTCCGCATCT CACAGAACTT CCTGTCCAGC ATCAATGAGG AGATCACACC CCGACGGCAG TCCATGACGG TGAAGAAGGG AGAGCACCGG CAGCTGCTGA AGGACAGCTT CATGGTGGAG CTGGTGGAGG GGGCCCGCAA GCTGCGCCAC GTCTTCCTGT TCACCGACCT GCTTCTCTGC ACCAAGCTCA AGAAGCAGAG CGGAGGCAAA ACGCAGCAGT ATGACTGCAA ATGGTACATT CCGCTCACGG ATCTCAGCTT CCAGATGGTG GATGAACTGG AGGCAGTGCC CAACATCCCC CTGGTGCCCG ATGAGGAGCT GGACGCTTTG AAGATCAAGA TCTCCCAGAT CAAGAATGAC ATCCAGAGAG AGAAGAGGGC GAACAAGGGC AGCAAGGCTA CGGAGAGGCT GAAGAAGAAG CTGTCGGAGC AGGAGTCACT GCTGCTGCTT ATGTCTCCCA GCATGGCCTT CAGGGTGCAC AGCCGCAACG GCAAGAGTTA CACGTTCCTG ATCTCCTCTG ACTATGAGCG TGCAGAGTGG AGGGAGAACA TCCGGGAGCA GCAGAAGAAG TGTTTCAGAA GCTTCTCCCT GACATCCGTG GAGCTGCAGA TGCTGACCAA CTCGTGTGTG AAACTCCAGA CTGTCCACAG CATTCCGCTG ACCATCAATA AGGAAGATGA TGAGTCTCCG GGGCTCTATG GGTTTCTGAA TGTCATCGTC CACTCAGCCA CTGGATTTAA GCAGAGTTCA AAAGCCCTTC AGCGGCCAGT AGCATCTGAC TTTGAGCCTC AGGGTCTGAG TGAAGCCGCT CGTTGGAACT CCAAGGAAAA CCTTCTCGCT GGACCCAGTG AAAATGACCC CAACCTTTTC GTTGCACTGT ATGATTTTGT GGCCAGTGGA GATAACACTC TAAGCATAAC TAAAGGTGAA AAGCTCCGGG TCTTAGGCTA TAATCACAAT GGGGAATGGT GTGAAGCCCA AACCAAAAAT GGCCAAGGCT GGGTCCCAAG CAACTACATC ACGCCAGTCA ACAGTCTGGA GAAACACTCC TGGTACCATG GGCCTGTGTC CCGCAATGCC GCTGAGTATC TGCTGAGCAG CGGGATCAAT GGCAGCTTCT TGGTGCGTGA GAGTGAGAGC AGTCCTGGCC AGAGGTCCAT CTCGCTGAGA TACGAAGGGA GGGTGTACCA TTACAGGATC AACACTGCTT CTGATGGCAA GCTCTACGTC TCCTCCGAGA GCCGCTTCAA CACCCTGGCC GAGTTGGTTC ATCATCATTC AACGGTGGCC GACGGGCTCA TCACCACGCT CCATTATCCA GCCCCAAAGC GCAACAAGCC CACTGTCTAT GGTGTGTCCC CCAACTACGA CAAGTGGGAG ATGGAACGCA CGGACATCAC CATGAAGCAC AAGCTGGGCG GGGGCCAGTA CGGGGAGGTG TACGAGGGCG TGTGGAAGAA ATACAGCCTG ACGGTGGCCG TGAAGACCTT GAAGGAGGAC ACCATGGAGG TGGAAGAGTT CTTGAAAGAA GCTGCAGTCA TGAAAGAGAT CAAACACCCT AACCTGGTGC AGCTCCTTGG GGTCTGCACC CGGGAGCCCC CGTTCTATAT CATCACTGAG TTCATGACCT ACGGGAACCT CCTGGACTAC CTGAGGGAGT GCAACCGGCA GGAGGTGAAC GCCGTGGTGC TGCTGTACAT GGCCACTCAG ATCTCGTCAG CCATGGAGTA CCTGGAGAAG AAAAACTTCA TCCACAGAGA TCTTGCTGCC CGAAACTGCC TGGTAGGGGA GAACCACTTG GTGAAGGTAG CTGATTTTGG CCTGAGCAGG TTGATGACAG GGGACACCTA CACAGCCCAT GCTGGAGCCA AGTTCCCCAT CAAATGGACT GCACCCGAGA GCCTGGCCTA CAACAAGTTC TCCATCAAGT CCGACGTCTG GGCATTTGGA GTATTGCTTT GGGAAATTGC TACCTATGGC ATGTCCCCTT ACCCGGGAAT TGACCTGTCC CAGGTGTATG AGCTGCTAGA GAAGGACTAC CGCATGGAGC GCCCAGAAGG CTGCCCAGAG AAGGTCTATG AACTCATGCG AGCATGTTGG CAGTGGAATC CCTCTGACCG GCCCTCCTTT GCTGAAATCC ACCAAGCCTT TGAAACAATG TTCCAGGAAT CCAGTATCTC AGACGAAGTG GAAAAGGAGC TGGGGAAACA AGGCGTCCGT GGGGCTGTGA GTACCTTGCT GCAGGCCCCA GAGCTGCCCA CCAAGACGAG GACCTCCAGG AGAGCTGCAG AGCACAGAGA CACCACTGAC GTGCCTGAGA TGCCTCACTC CAAGGGCCAG GGAGAGAGCG ATCCTCTGGA CCATGAGCCT GCCGTGTCTC CATTGCTCCC TCGAAAAGAG CGAGGTCCCC CGGAGGGCGG CCTGAATGAA GATGAGCGCC TTCTCCCCAA AGACAAAAAG ACCAACTTGT TCAGCGCCTT GATCAAGAAG AAGAAGAAGA CAGCCCCAAC CCCTCCCAAA CGCAGCAGCT CCTTCCGGGA GATGGACGGC CAGCCGGAGC GCAGAGGGGC CGGCGAGGAA GAGGGCCGAG ACATCAGCAA CGGGGCACTG GCTTTCACCC CCTTGGACAC AGCTGACCCA GCCAAGTCCC CAAAGCCCAG CAATGGGGCT GGGGTCCCCA ATGGAGCCCT CCGGGAGTCT GGGGGCTCAG GCTTCCGGTC TCCCCACCTG TGGAAGAAGT CCAGCACGCT GACCAGCAGC CGCCTAGCCA CCGGCGAGGA GGAGGGCGGT GGCAGCTCCA GCAAGCGCTT CCTGCGCTCT TGCTCCGCCT CCTGCGTTCC CCATGGGGCC AAGGACACGG AGTGGAGGTC AGTCACGCTG CCTCGGGACT TGCAGTCCAC GGGAAGACAG TTTGACTCGT CCACATTTGG AGGGCACAAA AGTGAGAAGC CGGCTCTGCC TCGGAAGAGG GCAGGGGAGA ACAGGTCTGA CCAGGTGACC CGAGGCACAG TAACGCCTCC CCCCAGGCTG GTGAAAAAGA ATGAGGAAGC TGCTGATGAG GTCTTCAAAG ACATCATGGA GTCCAGCCCG GGCTCCAGCC CGCCCAACCT GACTCCAAAA CCCCTCCGGC GGCAGGTCAC CGTGGCCCCT GCCTCGGGCC TCCCCCACAA GGAAGAAGCT GGAAAGGGCA GTGCCTTAGG GACCCCTGCT GCAGCTGAGC CAGTGACCCC CACCAGCAAA GCAGGCTCAG GTGCACCAGG GGGCACCAGC AAGGGCCCCG CCGAGGAGTC CAGAGTGAGG AGGCACAAGC ACTCCTCTGA GTCGCCAGGG AGGGACAAGG GGAAATTGTC CAGGCTCAAA CCTGCCCCGC CGCCCCCACC AGCAGCCTCT GCAGGGAAGG CTGGAGGAAA GCCCTCGCAG AGCCCGAGCC AGGAGGCGGC CGGGGAGGCA GTCCTGGGCG CAAAGACAAA AGCCACGAGT CTGGTTGATG CTGTGAACAG TGACGCTGCC AAGCCCAGCC AGCCGGGAGA GGGCCTCAAA AAGCCCGTGC TCCCGGCCAC TCCAAAGCCA CAGTCCGCCA AGCCGTCGGG GACCCCCATC AGCCCAGCCC CCGTTCCCTC CACGTTGCCA TCAGCATCCT CGGCCCTGGC AGGGGACCAG CCGTCTTCCA CCGCCTTCAT CCCTCTCATA TCAACCCGAG TGTCTCTTCG GAAAACCCGC CAGCCTCCAG AGCGGATCGC CAGCGGCGCC ATCACCAAGG GCGTGGTCCT GGACAGCACC GAGGCGCTGT GCCTCGCCAT CTCTAGGAAC TCCGAGCAGA TGGCCAGCCA CAGCGCAGTG CTGGAGGCCG GCAAAAACCT CTACACGTTC TGCGTGAGCT ATGTGGATTC CATCCAGCAA ATGAGGAACA AGTTTGCCTT CCGAGAGGCC ATCAACAAAC TGGAGAATAA TCTCCGGGAG CTTCAGATCT GCCCGGCGAC AGCAGGCAGT GGTCCAGCGG CCACTCAGGA CTTCAGCAAG CTCCTCAGTT CGGTGAAGGA AATCAGTGAC ATAGTGCAGA GGTAGGAATT CCGCCCCCCC CCCCTAACGT TACTGGCCGA AGCCGCTTGG AATAAGGCCG GTGTGCGTTT GTCTATATGT TATTTTCCAC CATATTGCCG TCTTTTGGCA ATGTGAGGGC CCGGAAACCT GGCCCTGTCT TCTTGACGAG CATTCCTAGG GGTCTTTCCC CTCTCGCCAA AGGAATGCAA GGTCTGTTGA ATGTCGTGAA GGAAGCAGTT CCTCTGGAAG CTTCTTGAAG ACAAACAACG TCTGTAGCGA CCCTTTGCAG GCAGCGGAAC CCCCCACCTG GCGACAGGTG CCTCTGCGGC CAAAAGCCAC GTGTATAAGA TACACCTGCA AAGGCGGCAC AACCCCAGTG CCACGTTGTG AGTTGGATAG TTGTGGAAAG AGTCAAATGG CTCTCCTCAA GCGTATTCAA CAAGGGGCTG AAGGATGCCC AGAAGGTACC CCATTGTATG GGATCTGATC TGGGGCCTCG GTGCACATGC TTTACATGTG TTTAGTCGAG GTTAAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCTGGCGAGG GCGAGGGCGA TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA GCAGAAGAAC GGCATCAAGG CGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT GTACAAGTAA TGAATTAATT AAGAATTATC AAGCTTATCG ATACCGTCGA CCTGCAGCCA AGCTTATCGA TAAAATAAAA GATTTTATTT AGTCTCCAGA AAAAGGGGGG AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT TTTGCAAGGC ATGGAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA GGTTAGGAAC AGAGAGACAG CAGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC TCAGGGCCAA GAACAGATGG TCCCCAGATG CGGTCCCGCC CTCAGCAGTT TCTAGAGAAC CATCAGATGT TTCCAGGGTG CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG GCGCGCCAGT CCTCCGATAG ACTGCGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG ACTACCCGTC AGCGGGGGTC TTTCATGGGT AACAGTTTCT TGAAGTTGGA GAACAACATT CTGAGGGTAG GAGTCGAATA TTAAGTAATC CTGACTCAAT TAGCCACTGT TTTGAATCCA CATACTCCAA TACTCCTGAA ATAGTTCATT ATGGACAGCG CAGAAAGAGC TGGGGAGAAT TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CTAAGAAACC ATTATTATCA TGACATTAAC CTATAAAAAT AGGCGTATCA CGAGGCCCTT TCGTCTCGCG CGTTTCGGTG ATGACGGTGA AAACCTCTGA CACATGCAGC TCCCGGAGAC GGTCACAGCT TGTCTGTAAG CGGATGCCGG GAGCAGACAA GCCCGTCAGG GCGCGTCAGC GGGTGTTGGC GGGTGTCGGG GCTGGCTTAA CTATGCGGCA TCAGAGCAGA TTGTACTGAG AGTGCACCAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT ACCGCATCAG GCGCCATTCG CCATTCAGGC TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC GTTGTAAAAC GACGGCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGCGA TTAGTCCAAT TTGTTAAAGA CAGGATATCA GTGGTCCAGG CTCTAGTTTT GACTCAACAA TATCACCAGC TGAAGCCTAT AGAGTACGAG CCATAGATAA AATAAAAGAT TTTATTTAGT CTCCAGAAAA AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCCGCCCTC AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATAGACT GCGTCGCCCG GGTACCCGTA TTCCCAATAA AGCCTCTTGC TGTTTGCATC CGAATCGTGG ACTCGCTGAT CCTTGGGAGG GTCTCCTCAG ATTGATTGAC TGCCCACCTC GGGGGTCTTT CATTTGGAGG TTCCACCGAG ATTTGGAGAC CCCTGCCCAG GGACCACCGA CCCCCCCGCC GGGAGGTAAG CTGGCCAGCG GTCGTTTCGT GTCTGTCTCT GTCTTTGTGC GTGTTTGTGC CGGCATCTAA TGTTTGCGCC TGCGTCTGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT GGAACTGACG AGTTCTGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTTGGG GGCCGTTTTT GTGGCCCGAC CTGAGGAAGG GAGTCGATGT GGAATCCGAC CCCGTCAGGA TATGTGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCTCCGTC TGAATTTTTG CTTTCGGTTT GGAACCGAAG CCGCGCGTCT TGTCTGCTGC AGCGCTGCAG CATCGTTCTG TGTTGTCTCT GTCTGACTGT GTTTCTGTAT TTGTCTGAAA ATTAGGGCCA GACTGTTACC ACTCCCTTAA GTTTGACCTT AGGTCACTGG AAAGATGTCG AGCGGATCGC TCACAACCAG TCGGTAGATG TCAAGAAGAG ACGTTGGGTT ACCTTCTGCT CTGCAGAATG GCCAACCTTT AACGTCGGAT GGCCGCGAGA CGGCACCTTT AACCGAGACC TCATCACCCA GGTTAAGATC AAGGTCTTTT CACCTGGCCC GCATGGACAC CCAGACCAGG TCCCCTACAT CGTGACCTGG GAAGCCTTGG CTTTTGACCC CCCTCCCTGG GTCA
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