pPIC3.5K-AOX1-EGFP
$113.00
In stock
SKU
PL65157
100μg liquid plasmid(Instantly):$134
| name | pPIC3.5K-AOX1-EGFP | another name | apPIC3.5K-AOX1-EGFP |
| Insert Size (bp) | Plasmid host | Yeast bacterium | |
| Plasmid uses | Protein expression | Fragment type | ORF |
| Fragment species | Prokaryotic resistance | Amp | |
| Selective marker | HIS4 | Fluorescent labeling | Green |
| Promoter | AOX1 | Replicon | pBR322 |
| Competence | DH5a | Growth Temperature | 37°C |
| Vector backbone | 5′ sequencing primer | ||
| 3′ sequencing primer | Copy number | high | |
| Induction methodanother name |
1. All products on this platform are only for scientific research and cannot be used for clinical trials;
2. The small package of plasmids is sold at the price of the template, which only guarantees the correct sequencing of the gene part, and does not guarantee the experimental effect such as the expression of the plasmid;
3. Some plasmids have not been completely sequenced even if they are produced by the company, and if the sequence sequencing of key parts is correct, it is also considered to be the correct plasmid;
4. The after-sales period of small package plasmid products is 3 months from the date you receive the product.
5. Plasmid construction is charged at cost, all plasmids will be shared with other experimenters, please inform and pay confidential fees in advance for confidential plasmids;
6. Because scientific research is an activity to explore the unknown, there is great uncertainty, so Biogege does not bear additional joint and several liability under any circumstances;
7. Publication [Chinese papers] please mark: product name (product item number) provided by Biogege;
8. Please mark Product Name (Product Item Number) was obtained from Biogege,China.
Instructions
Plasmid dry powder (stored at minus 20 degrees, shelf life 90 days, transportation at room temperature)
1. After receiving the dry powder plasmid, please centrifuge at 5000rpm for 1min, and then add 20μl of sterile water to dissolve the plasmid;
2. Thaw the corresponding competent on an ice box, take 2 μl of plasmid and add it to 100 μl of competent for 30min;
3. 42 degrees heat shock for 60s, ice bath for 2min, add 900 of antibiotic-free LB medium, and culture at 180rpm shaking for 45min;
4. Centrifuge at 6000rpm for 3min, leave 100μl of supernatant to resuspend, and then coat it on the corresponding resistant LB plate;
5.After inverting overnight, if there are too many colonies, the plasmid will be diluted for transformation, and if there are no colonies, 10ul plasmid will be taken for transformation;
6. Pick single colonies into the corresponding resistant LB liquid medium overnight shaking culture, and extract the plasmid as needed.
Liquid plasmid (minus 20 degrees storage, shelf life 90 days, ice pack transportation).
1. After receiving the liquid plasmid, please shake the preservation tube so that the liquid plasmid does not adsorb on the tube wall;
2.Take an appropriate amount of plasmid according to the experimental needs, and store the rest in a minus 20 degree freezer.
Glycerol strains (stored at minus 80 degrees, shelf life of 90 days, ice pack transportation).
1. After receiving the glycerol strain, please shake the preservation tube so that the bacterial solution does not adsorb on the tube wall;
2. Use the inoculation loop to mark the line in four areas on the solid plate, and add the corresponding resistance if there is resistance;
3. Inverted culture in constant temperature incubator for 12~16h;
4. Be sure to pick a single colony and inoculate it in the liquid medium, and add the corresponding resistance if there is resistance;
5. Shake the shaker and culture overnight, and culture the strains according to the needs of the experiment.
AGATCTAACA TCCAAAGACG AAAGGTTGAA TGAAACCTTT TTGCCATCCG ACATCCACAG GTCCATTCTC ACACATAAGT GCCAAACGCA ACAGGAGGGG ATACACTAGC AGCAGACCGT TGCAAACGCA GGACCTCCAC TCCTCTTCTC CTCAACACCC ACTTTTGCCA TCGAAAAACC AGCCCAGTTA TTGGGCTTGA TTGGAGCTCG CTCATTCCAA TTCCTTCTAT TAGGCTACTA ACACCATGAC TTTATTAGCC TGTCTATCCT GGCCCCCCTG GCGAGGTTCA TGTTTGTTTA TTTCCGAATG CAACAAGCTC CGCATTACAC CCGAACATCA CTCCAGATGA GGGCTTTCTG AGTGTGGGGT CAAATAGTTT CATGTTCCCC AAATGGCCCA AAACTGACAG TTTAAACGCT GTCTTGGAAC CTAATATGAC AAAAGCGTGA TCTCATCCAA GATGAACTAA GTTTGGTTCG TTGAAATGCT AACGGCCAGT TGGTCAAAAA GAAACTTCCA AAAGTCGCCA TACCGTTTGT CTTGTTTGGT ATTGATTGAC GAATGCTCAA AAATAATCTC ATTAATGCTT AGCGCAGTCT CTCTATCGCT TCTGAACCCC GGTGCACCTG TGCCGAAACG CAAATGGGGA AACACCCGCT TTTTGGATGA TTATGCATTG TCTCCACATT GTATGCTTCC AAGATTCTGG TGGGAATACT GCTGATAGCC TAACGTTCAT GATCAAAATT TAACTGTTCT AACCCCTACT TGACAGCAAT ATATAAACAG AAGGAAGCTG CCCTGTCTTA AACCTTTTTT TTTATCATCA TTATTAGCTT ACTTTCATAA TTGCGACTGG TTCCAATTGA CAAGCTTTTG ATTTTAACGA CTTTTAACGA CAACTTGAGA AGATCAAAAA ACAACTAATT ATTCGAAGGA TCCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAGCGGCCG CGAATTAATT CGCCTTAGAC ATGACTGTTC CTCAGTTCAA GTTGGGCACT TACGAGAAGA CCGGTCTTGC TAGATTCTAA TCAAGAGGAT GTCAGAATGC CATTTGCCTG AGAGATGCAG GCTTCATTTT TGATACTTTT TTATTTGTAA CCTATATAGT ATAGGATTTT TTTTGTCATT TTGTTTCTTC TCGTACGAGC TTGCTCCTGA TCAGCCTATC TCGCAGCTGA TGAATATCTT GTGGTAGGGG TTTGGGAAAA TCATTCGAGT TTGATGTTTT TCTTGGTATT TCCCACTCCT CTTCAGAGTA CAGAAGATTA AGTGAGAAGT TCGTTTGTGC AAGCTTATCG ATAAGCTTTA ATGCGGTAGT TTATCACAGT TAAATTGCTA ACGCAGTCAG GCACCGTGTA TGAAATCTAA CAATGCGCTC ATCGTCATCC TCGGCACCGT CACCCTGGAT GCTGTAGGCA TAGGCTTGGT TATGCCGGTA CTGCCGGGCC TCTTGCGGGA TATCGTCCAT TCCGACAGCA TCGCCAGTCA CTATGGCGTG CTGCTAGCGC TATATGCGTT GATGCAATTT CTATGCGCAC CCGTTCTCGG AGCACTGTCC GACCGCTTTG GCCGCCGCCC AGTCCTGCTC GCTTCGCTAC TTGGAGCCAC TATCGACTAC GCGATCATGG CGACCACACC CGTCCTGTGG ATCTATCGAA TCTAAATGTA AGTTAAAATC TCTAAATAAT TAAATAAGTC CCAGTTTCTC CATACGAACC TTAACAGCAT TGCGGTGAGC ATCTAGACCT TCAACAGCAG CCAGATCCAT CACTGCTTGG CCAATATGTT TCAGTCCCTC AGGAGTTACG TCTTGTGAAG TGATGAACTT CTGGAAGGTT GCAGTGTTAA CTCCGCTGTA TTGACGGGCA TATCCGTACG TTGGCAAAGT GTGGTTGGTA CCGGAGGAGT AATCTCCACA ACTCTCTGGA GAGTAGGCAC CAACAAACAC AGATCCAGCG TGTTGTACTT GATCAACATA AGAAGAAGCA TTCTCGATTT GCAGGATCAA GTGTTCAGGA GCGTACTGAT TGGACATTTC CAAAGCCTGC TCGTAGGTTG CAACCGATAG GGTTGTAGAG TGTGCAATAC ACTTGCGTAC AATTTCAACC CTTGGCAACT GCACAGCTTG GTTGTGAACA GCATCTTCAA TTCTGGCAAG CTCCTTGTCT GTCATATCGA CAGCCAACAG AATCACCTGG GAATCAATAC CATGTTCAGC TTGAGACAGA AGGTCTGAGG CAACGAAATC TGGATCAGCG TATTTATCAG CAATAACTAG AACTTCAGAA GGCCCAGCAG GCATGTCAAT ACTACACAGG GCTGATGTGT CATTTTGAAC CATCATCTTG GCAGCAGTAA CGAACTGGTT TCCTGGACCA AATATTTTGT CACACTTAGG AACAGTTTCT GTTCCGTAAG CCATAGCAGC TACTGCCTGG GCGCCTCCTG CTAGCACGAT ACACTTAGCA CCAACCTTGT GGGCAACGTA GATGACTTCT GGGGTAAGGG TACCATCCTT CTTAGGTGGA GATGCAAAAA CAATTTCTTT GCAACCAGCA ACTTTGGCAG GAACACCCAG CATCAGGGAA GTGGAAGGCA GAATTGCGGT TCCACCAGGA ATATAGAGGC CAACTTTCTC AATAGGTCTT GCAAAACGAG AGCAGACTAC ACCAGGGCAA GTCTCAACTT GCAACGTCTC CGTTAGTTGA GCTTCATGGA ATTTCCTGAC GTTATCTATA GAGAGATCAA TGGCTCTCTT AACGTTATCT GGCAATTGCA TAAGTTCCTC TGGGAAAGGA GCTTCTAACA CAGGTGTCTT CAAAGCGACT CCATCAAACT TGGCAGTTAG TTCTAAAAGG GCTTTGTCAC CATTTTGACG AACATTGTCG ACAATTGGTT TGACTAATTC CATAATCTGT TCCGTTTTCT GGATAGGACG ACGAAGGGCA TCTTCAATTT CTTGTGAGGA GGCCTTAGAA ACGTCAATTT TGCACAATTC AATACGACCT TCAGAAGGGA CTTCTTTAGG TTTGGATTCT TCTTTAGGTT GTTCCTTGGT GTATCCTGGC TTGGCATCTC CTTTCCTTCT AGTGACCTTT AGGGACTTCA TATCCAGGTT TCTCTCCACC TCGTCCAACG TCACACCGTA CTTGGCACAT CTAACTAATG CAAAATAAAA TAAGTCAGCA CATTCCCAGG CTATATCTTC CTTGGATTTA GCTTCTGCAA GTTCATCAGC TTCCTCCCTA ATTTTAGCGT TCAACAAAAC TTCGTCGTCA AATAACCGTT TGGTATAAGA ACCTTCTGGA GCATTGCTCT TACGATCCCA CAAGGTGGCT TCCATGGCTC TAAGACCCTT TGATTGGCCA AAACAGGAAG TGCGTTCCAA GTGACAGAAA CCAACACCTG TTTGTTCAAC CACAAATTTC AAGCAGTCTC CATCACAATC CAATTCGATA CCCAGCAACT TTTGAGTTGC TCCAGATGTA GCACCTTTAT ACCACAAACC GTGACGACGA GATTGGTAGA CTCCAGTTTG TGTCCTTATA GCCTCCGGAA TAGACTTTTT GGACGAGTAC ACCAGGCCCA ACGAGTAATT AGAAGAGTCA GCCACCAAAG TAGTGAATAG ACCATCGGGG CGGTCAGTAG TCAAAGACGC CAACAAAATT TCACTGACAG GGAACTTTTT GACATCTTCA GAAAGTTCGT ATTCAGTAGT CAATTGCCGA GCATCAATAA TGGGGATTAT ACCAGAAGCA ACAGTGGAAG TCACATCTAC CAACTTTGCG GTCTCAGAAA AAGCATAAAC AGTTCTACTA CCGCCATTAG TGAAACTTTT CAAATCGCCC AGTGGAGAAG AAAAAGGCAC AGCGATACTA GCATTAGCGG GCAAGGATGC AACTTTATCA ACCAGGGTCC TATAGATAAC CCTAGCGCCT GGGATCATCC TTTGGACAAC TCTTTCTGCC AAATCTAGGT CCAAAATCAC TTCATTGATA CCATTATTGT ACAACTTGAG CAAGTTGTCG ATCAGCTCCT CAAATTGGTC CTCTGTAACG GATGACTCAA CTTGCACATT AACTTGAAGC TCAGTCGATT GAGTGAACTT GATCAGGTTG TGCAGCTGGT CAGCAGCATA GGGAAACACG GCTTTTCCTA CCAAACTCAA GGAATTATCA AACTCTGCAA CACTTGCGTA TGCAGGTAGC AAGGGAAATG TCATACTTGA AGTCGGACAG TGAGTGTAGT CTTGAGAAAT TCTGAAGCCG TATTTTTATT ATCAGTGAGT CAGTCATCAG GAGATCCTCT ACGCCGGACG CATCGTGGCC GACCTGCAGG GGGGGGGGGG GCGCTGAGGT CTGCCTCGTG AAGAAGGTGT TGCTGACTCA TACCAGGCCT GAATCGCCCC ATCATCCAGC CAGAAAGTGA GGGAGCCACG GTTGATGAGA GCTTTGTTGT AGGTGGACCA GTTGGTGATT TTGAACTTTT GCTTTGCCAC GGAACGGTCT GCGTTGTCGG GAAGATGCGT GATCTGATCC TTCAACTCAG CAAAAGTTCG ATTTATTCAA CAAAGCCGCC GTCCCGTCAA GTCAGCGTAA TGCTCTGCCA GTGTTACAAC CAATTAACCA ATTCTGATTA TTCCTTTGCC CTCGGACGAG TGCTGGGGCG TCGGTTTCCA CTATCGGCGA GTACTTCTAC ACAGCCATCG GTCCAGACGG CCGCGCTTCT GCGGGCGATT TGTGTACGCC CGACAGTCCC GGCTCCGGAT CGGACGATTG CGTCGCATCG ACCCTGCGCC CAAGCTGCAT CATCGAAATT GCCGTCAACC AAGCTCTGAT AGAGTTGGTC AAGACCAATG CGGAGCATAT ACGCCCGGAG CCGCGGCGAT CCTGCAAGCT CCGGATGCCT CCGCTCGAAG TAGCGCGTCT GCTGCTCCAT ACAAGCCAAC CACGGCCTCC AGAAGAAGAT GTTGGCGACC TCGTATTGGG AATCCCCGAA CATCGCCTCG CTCCAGTCAA TGACCGCTGT TATGCGGCCA TTGTCCGTCA GGACATTGTT GGAGCCGAAA TCCGCGTGCA CGAGGTGCCG GACTTCGGGG CAGTCCTCGG CCCAAAGCAT CAGCTCATCG AGAGCCTGCG CGACGGACGC ACTGACGGTG TCGTCCATCA CAGTTTGCCA GTGATACACA TGGGGATCAG CAATCGCGCA TATGAAATCA CGCCATGTAG TGTATTGACC GATTCCTTGC GGTCCGAATG GGCCGAACCC GCTCGTCTGG CTAAGATCGG CCGCAGCGAT CGCATCCATG GCCTCCGCGA CCGGCTGCAG AACAGCGGGC AGTTCGGTTT CAGGCAGGTC TTGCAACGTG ACACCCTGTG CACGGCGGGA GATGCAATAG GTCAGGCTCT CGCTGAATTC CCCAATGTCA AGCACTTCCG GAATCGGGAG CGCGGCCGAT GCAAAGTGCC GATAAACATA ACGATCTTTG TAGAAACCAT CGGCGCAGCT ATTTACCCGC AGGACATATC CACGCCCTCC TACATCGAAG CTGAAAGCAC GAGATTCTTC GCCCTCCGAG AGCTGCATCA GGTCGGAGAC GCTGTCGAAC TTTTCGATCA GAAACTTCTC GACAGACGTC GCGGTGAGTT CAGGCTTTTT ACCCATAACA CCCCTTGTAT TACTGTTTAT GTAAGCAGAC AGTTTTATTG TTCATGATGA TATATTTTTA TCTTGTGCAA TGTAACATCA GAGATTTTGA GACACAACGT GGCTTTCCCC CCCCCCCCTG CAGGTCGGCA TCACCGGCGC CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG GGGACTGTTG GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGAGT ATCTATGATT GGAAGTATGG GAATGGTGAT ACCCGCATTC TTCAGTGTCT TGAGGTCTCC TATCAGATTA TGCCCAACTA AAGCAACCGG AGGAGGAGAT TTCATGGTAA ATTTCTCTGA CTTTTGGTCA TCAGTAGACT CGAACTGTGA GACTATCTCG GTTATGACAG CAGAAATGTC CTTCTTGGAG ACAGTAAATG AAGTCCCACC AATAAAGAAA TCCTTGTTAT CAGGAACAAA CTTCTTGTTT CGAACTTTTT CGGTGCCTTG AACTATAAAA TGTAGAGTGG ATATGTCGGG TAGGAATGGA GCGGGCAAAT GCTTACCTTC TGGACCTTCA AGAGGTATGT AGGGTTTGTA GATACTGATG CCAACTTCAG TGACAACGTT GCTATTTCGT TCAAACCATT CCGAATCCAG AGAAATCAAA GTTGTTTGTC TACTATTGAT CCAAGCCAGT GCGGTCTTGA AACTGACAAT AGTGTGCTCG TGTTTTGAGG TCATCTTTGT ATGAATAAAT CTAGTCTTTG ATCTAAATAA TCTTGACGAG CCAAGGCGAT AAATACCCAA ATCTAAAACT CTTTTAAAAC GTTAAAAGGA CAAGTATGTC TGCCTGTATT AAACCCCAAA TCAGCTCGTA GTCTGATCCT CATCAACTTG AGGGGCACTA TCTTGTTTTA GAGAAATTTG CGGAGATGCG ATATCGAGAA AAAGGTACGC TGATTTTAAA CGTGAAATTT ATCTCAAGAT CTCTGCCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA TAGCGGAGTG TATACTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT CAGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTGCAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAACACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT TCAAGAATTA ATTCTCATGT TTGACAGCTT ATCATCGATA AGCTGACTCA TGTTGGTATT GTGAAATAGA CGCAGATCGG GAACACTGAA AAATAACAGT TATTATTCG
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